BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,926,894	T→G	5.1%	D649A (GAC→GCC)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,894	0	T	G	5.1%	450.9 / 11.7	157	D649A (GAC→GCC)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base T (67/82); new base G (6/2); total (73/84)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

Only 100 of 157 total aligned reads displayed.

GCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTGACG  >  NC_000913/1926746‑1927040

gCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                                                                                                                                                   <  1:1027509/150‑1 (MQ=255)

 cGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                                                                  >  1:743032/1‑150 (MQ=255)

 cGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCAACGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                                                                  >  2:1813385/1‑150 (MQ=255)

  gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                                 <  1:286393/150‑1 (MQ=255)

    ttAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATg                                                                                                                                               <  1:703115/150‑1 (MQ=255)

    ttAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATg                                                                                                                                               >  2:1417411/1‑150 (MQ=255)

      aaCACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTc                                                                                                                                             <  1:591114/150‑1 (MQ=255)

      aaCACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTc                                                                                                                                             <  1:1690550/150‑1 (MQ=255)

       acacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGACTGAGTCCATGTCg                                                                                                                                            >  1:52708/1‑150 (MQ=255)

       acacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGAGCCAGCGCGACCAGAAAAGAATATTCCATCGCTACGACTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGACTTAGTCCAAGTCg                                                                                                                                            >  2:629322/1‑150 (MQ=255)

       acacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTACCCTGCGCCAGCGCGACCAGAAAAGAATATTACATCGCTACGACTTCGTACGATTTAAAGCGACAAGATTTACCGCCATGTCCTGAGTCCAAGTct                                                                                                                                            >  2:637754/1‑149 (MQ=255)

        cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATGGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCTTGGCCTGAGTCCATGTCgg                                                                                                                                           <  1:1103817/150‑1 (MQ=255)

        cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                           <  1:76789/150‑1 (MQ=255)

        cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                           <  2:1147651/150‑1 (MQ=255)

        cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTAAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                           <  2:131989/150‑1 (MQ=255)

         acTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGt                                                                                                                                          <  1:1755001/150‑1 (MQ=255)

           tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                                                                                        >  2:1605534/1‑150 (MQ=255)

            gAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                                                                       >  1:428744/1‑150 (MQ=255)

             aaaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag                                                                                                                                      <  2:454656/150‑1 (MQ=255)

              aaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                                                                                     <  1:1983261/150‑1 (MQ=255)

              aaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatc                                                                                                                                     <  2:343743/150‑5 (MQ=255)

                cATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTTAGTCCATGTCGGTACAGAGc                                                                                                                                   <  1:380833/150‑1 (MQ=255)

                 aTTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCa                                                                                                                                  <  2:1345801/150‑1 (MQ=255)

                   tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCAGATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGCTACAGAGCatt                                                                                                                                >  1:99904/1‑148 (MQ=255)

                      cTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaa                                                                                                                             >  1:741907/1‑150 (MQ=255)

                       tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                                                                            <  2:1961258/150‑1 (MQ=255)

                       tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                                                                            >  1:1872680/1‑150 (MQ=255)

                           aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                        <  2:1004916/150‑1 (MQ=255)

                            aaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                                                                                       <  1:705833/150‑1 (MQ=255)

                             aTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGt                                                                                                                      >  2:1791390/1‑150 (MQ=255)

                                cTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATATAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGGAATAAAAGAAGGTCAt                                                                                                                   >  2:482847/1‑150 (MQ=255)

                                    gTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGt                                                                                                               >  2:29143/1‑150 (MQ=255)

                                     tCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATAATCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGtt                                                                                                              <  1:877552/150‑1 (MQ=255)

                                      ccGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGttt                                                                                                             >  1:1362774/1‑150 (MQ=255)

                                      ccGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGttt                                                                                                             <  1:1093692/150‑1 (MQ=255)

                                       cGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGtttt                                                                                                            >  2:1471250/1‑150 (MQ=255)

                                         cAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                                                                                          <  1:900996/150‑1 (MQ=255)

                                         aaGGGGTTGCTGGTAATGTTCCCTGCGCAATAGCGACCAGAAAAGCATATTCCATCGCTTGGCCTTCGTACGATTTAAAGCGACCAGTTTTAGCGCCATGGCCTGAGTCCATGTCGGTACAGAGCTATATAAGATGGTCATCGGTTTTCa                                                                                                          <  2:1780077/149‑1 (MQ=255)

                                           ggCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGAATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGc                                                                                                        <  1:1752374/150‑1 (MQ=255)

                                              gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcg                                                                                                     >  1:426411/1‑150 (MQ=255)

                                              gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAGAAAAGATGGTCATCGGTTTTCAGCTcg                                                                                                     >  1:1790709/1‑150 (MQ=255)

                                                tgCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCATATTTACCGCCTTGGCCTGAGTCCTTGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                                   <  1:185891/149‑1 (MQ=255)

                                                aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                                   >  1:1807339/1‑150 (MQ=255)

                                                       aaTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAATATGGTCATCGGTTTTCAGCTCGCGCAATTTa                                                                                            >  1:1883816/1‑150 (MQ=255)

                                                        aTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTGCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAg                                                                                           <  1:602028/150‑1 (MQ=255)

                                                         tGTTCCCTTCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                                                          <  2:946450/150‑1 (MQ=255)

                                                            tCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                                                                                       <  1:698071/150‑1 (MQ=255)

                                                                   gcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAtttt                                                                                <  2:797072/150‑1 (MQ=255)

                                                                     cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGc                                                                              <  2:1169394/150‑1 (MQ=255)

                                                                     cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGc                                                                              <  1:1201749/150‑1 (MQ=255)

                                                                        cgcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCgg                                                                           <  2:1871140/150‑1 (MQ=255)

                                                                           gACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGAATTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc                                                                        >  2:575365/1‑150 (MQ=255)

                                                                            aCCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTcc                                                                       <  1:294542/150‑1 (MQ=255)

                                                                             ccAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                      <  1:2015064/150‑1 (MQ=255)

                                                                              cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGCCCCATTTTGCCGGTTCCCa                                                                     >  1:1320825/1‑150 (MQ=255)

                                                                                gAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat                                                                   <  2:1578963/150‑1 (MQ=255)

                                                                                     gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc                                                              <  1:1605534/150‑1 (MQ=255)

                                                                                     gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc                                                              <  1:1032516/150‑1 (MQ=255)

                                                                                      cATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCa                                                             >  1:500839/1‑150 (MQ=255)

                                                                                       atatTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCAc                                                            >  1:181551/1‑150 (MQ=255)

                                                                                          ttCCATCGCTACGCCTTCGTACTATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGATCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTg                                                         <  2:1090522/150‑1 (MQ=255)

                                                                                          ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTg                                                         <  2:1747780/150‑1 (MQ=255)

                                                                                            ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgag                                                       >  1:1317695/1‑150 (MQ=255)

                                                                                            ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgag                                                       <  1:575802/150‑1 (MQ=255)

                                                                                               tCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAAt                                                    >  1:1560100/1‑150 (MQ=255)

                                                                                                 gCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGACTCCATGTAGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCACGCAATTTAGAGACCCAATTTGACGGTTCCCAATATTGCACCTGAGAATCg                                                  >  1:1263770/1‑150 (MQ=255)

                                                                                                  cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCTTGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGt                                                 <  1:1572343/150‑1 (MQ=255)

                                                                                                  cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGt                                                 >  2:130874/1‑150 (MQ=255)

                                                                                                   tACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTg                                                <  2:3686/150‑1 (MQ=255)

                                                                                                    aCGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGc                                               <  2:1900117/150‑1 (MQ=255)

                                                                                                    aCGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGc                                               <  1:1366390/150‑1 (MQ=255)

                                                                                                    aCGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGCCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTATCAGCTCGCGCAATCTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGc                                               <  2:1400383/150‑1 (MQ=255)

                                                                                                     cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCTATAAAAGATGGTCATCTGTTTTCAGCTCGAGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCa                                              <  1:154174/150‑1 (MQ=255)

                                                                                                     cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCa                                              >  1:1488879/1‑150 (MQ=255)

                                                                                                           cGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCgg                                        <  2:448405/150‑1 (MQ=255)

                                                                                                           cGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCgg                                        >  1:1781197/1‑150 (MQ=255)

                                                                                                                 aTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTa                                  <  2:1814119/150‑1 (MQ=255)

                                                                                                                  tttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGACGTTaa                                 >  1:1664528/1‑149 (MQ=255)

                                                                                                                   ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  1:1299752/150‑1 (MQ=255)

                                                                                                                   ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  1:1471250/150‑1 (MQ=255)

                                                                                                                   ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  1:520304/150‑1 (MQ=255)

                                                                                                                       aGCGACCACATTTACCGCCAAGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAGCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTa                            <  1:1231576/150‑1 (MQ=255)

                                                                                                                        gCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTaa                           >  1:485222/1‑150 (MQ=255)

                                                                                                                         cGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTaaa                          >  1:881041/1‑150 (MQ=255)

                                                                                                                           aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGTTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATg                        <  1:637754/150‑1 (MQ=255)

                                                                                                                           aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGCCCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATg                        >  2:1923600/1‑150 (MQ=255)

                                                                                                                           aCCAGATTTACAGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCATCTCGCGCATTTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATg                        <  1:629322/150‑1 (MQ=255)

                                                                                                                            ccAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGc                       >  1:1583602/1‑150 (MQ=255)

                                                                                                                             cacaTTTACCCCCATGGCCTGAGTCCATGTAGGAACAGAGCCATAAAACATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTGGCCGGTTCCCAATATTGCACCAGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCg                      <  2:1254959/147‑1 (MQ=255)

                                                                                                                               gATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGa                    <  1:752646/150‑1 (MQ=255)

                                                                                                                                 tttACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATa                  <  2:741907/150‑1 (MQ=255)

                                                                                                                                 tgtACCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATa                  >  1:1697264/3‑150 (MQ=255)

                                                                                                                                    aCCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc               >  2:168505/1‑150 (MQ=255)

                                                                                                                                    aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGCCCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc               >  1:1261080/1‑150 (MQ=255)

                                                                                                                                    aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc               >  2:1674362/1‑150 (MQ=255)

                                                                                                                                     ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGGTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc              >  2:144767/1‑150 (MQ=255)

                                                                                                                                       gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg            >  2:1093604/1‑150 (MQ=255)

                                                                                                                                            ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCgg       <  2:570769/150‑1 (MQ=255)

                                                                                                                                            ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCgg       >  2:1397207/1‑150 (MQ=255)

                                                                                                                                            ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCgg       >  2:1389372/1‑150 (MQ=255)

GCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTGACG  >  NC_000913/1926746‑1927040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`