Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,937 A→G 8.8% intergenic (+45/+17) tyrP → / ← yecA tyrosine transporter/UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,9370AG 8.8% 252.9 / 12.7 91intergenic (+45/+17)tyrP/yecAtyrosine transporter/UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base A (39/44);  new base G (4/4);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

TCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATG  >  NC_000913/1990789‑1991085
                                                                                                                                                    |                                                                                                                                                    
gcAAAGGTGGTCGGCCGGCGCGGGTGGTGGTGTTTCTCTGGGGTATTGCGGGGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGCAGTGGGGGGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGac                                                                                                                                                     <  2:1755950/149‑1 (MQ=255)
 cAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGaca                                                                                                                                                    >  1:1051616/1‑150 (MQ=255)
  aaaGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGAATTTTTTGATTGCGGCAGGGTTTTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGacac                                                                                                                                                   <  1:1200560/150‑1 (MQ=255)
    aGGTGGTCGTCCGGCGCTGGTGGTGGTGGTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAccc                                                                                                                                                 <  2:1334183/150‑1 (MQ=255)
         gtcgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaa                                                                                                                                            <  1:2017171/150‑3 (MQ=255)
          tcgtcCTGCGCTGGATGTTGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTTCAATTTTTGATTGCTTCAGTGTTGTTACCAGAAGTGGAGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                           <  1:34250/150‑4 (MQ=255)
          tcgtcCGGCGCTGGTGGGGGGGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCTGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaag                                                                                                                                           <  1:1473123/150‑4 (MQ=255)
           cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTgg                                                                                                                                          <  2:615146/150‑1 (MQ=255)
           cgtcCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCCGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGTCAGCGTTAATTCTGGCACCCAAtaagg                                                                                                                                          <  1:205128/150‑5 (MQ=38)
             tcCGGCGCTGGTGGGTGTGTTTCTCTGTGGTATTGCTGGGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGcc                                                                                                                                        <  2:1316411/150‑1 (MQ=255)
             gccGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGcc                                                                                                                                        <  1:15995/149‑1 (MQ=255)
                   gcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTaa                                                                                                                                  <  1:1965718/150‑1 (MQ=255)
                   gcTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTaa                                                                                                                                  <  1:1076629/150‑1 (MQ=255)
                    cTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAAt                                                                                                                                 <  1:37747/150‑1 (MQ=255)
                     tggtggtggTGTTTCTATGTGGTATTGCTGTGCTGGGCGTTCAATTTTTGATTGCGGGAGGGGTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGTGAGACAGAATTAACGCTGACACCCAATTTGGCCTCTTAATg                                                                                                                                <  1:920130/150‑1 (MQ=255)
                      ggtggtggtGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgc                                                                                                                               >  2:1407563/1‑150 (MQ=255)
                       gtggttgtgTTTCTCTGTGGTATTGCGGTGATTGGCGTGGAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGTTGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                              <  2:1438734/150‑1 (MQ=255)
                       gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATATGGCCTCTAAATgca                                                                                                                              >  2:436062/1‑150 (MQ=255)
                       gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATATTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgca                                                                                                                              >  1:1528805/1‑150 (MQ=255)
                         ggtggtgTTTCTCTGTGGTATTGCTGTGCTTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGCTATCCTCAAATTCCTTATTGGTTGCCAGAATTAACGATGACACCAATTTTGGCCTCTTAATgcagg                                                                                                                            >  2:1083081/1‑150 (MQ=255)
                               gtTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGCGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAAATTGGCCTCTTAATGCAGGCAGCAc                                                                                                                      >  2:239645/1‑150 (MQ=255)
                                 ttCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGAGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTg                                                                                                                    <  1:391917/150‑1 (MQ=255)
                                  tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGTGTGATCAGATAGCCTCAAATTCCTTATTTGGTGCCAGAATTAAAGCTGACACACAATTTGGCCTCTTAATGCAGGCAGCACTga                                                                                                                   >  1:227168/1‑149 (MQ=255)
                                  tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGc                                                                                                                   <  1:1906931/150‑1 (MQ=255)
                                  tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGc                                                                                                                   <  1:1573508/150‑1 (MQ=255)
                                   ctctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCt                                                                                                                  >  2:620183/1‑150 (MQ=255)
                                   ctctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACAAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGACAGATTTAACGCTGACACCAAATTTGGCCTCTTAATGCAGGCAGCAATgat                                                                                                                  >  1:1283055/1‑148 (MQ=255)
                                     ctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTa                                                                                                                <  2:5330/150‑1 (MQ=255)
                                      gggggTATGGCGGTGATTGGCGTGCAATGTTTGATTGCGGCAGGGGGGGTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTaa                                                                                                               <  1:1285102/147‑1 (MQ=255)
                                         ggTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAtt                                                                                                            <  2:405673/150‑1 (MQ=255)
                                          gTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAAttt                                                                                                           >  1:1170099/1‑150 (MQ=255)
                                           tctagCTGTGATTGGCGTGCAATTTTTGATAGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATACCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTc                                                                                                          <  2:1022368/146‑1 (MQ=255)
                                           tATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCATGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTc                                                                                                          <  1:1789674/150‑1 (MQ=255)
                                           gaTTGCTCTGATTGGCGTGCAATGTTTGATTGCGGCAGGGGGGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTc                                                                                                          <  1:388602/149‑1 (MQ=255)
                                            aTTGCTGTGATTGGCGTGCAATTTTTGTTTGCGGCAGGGGTGTTACCAGAAGTGGGGCGAGCCTATACCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCt                                                                                                         <  2:284070/150‑1 (MQ=255)
                                            aTTGCTGGGATTGGCGTGCAATTTTTGATTGCGGCAGGGGTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGGGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCt                                                                                                         <  1:1270690/150‑1 (MQ=255)
                                             ttGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCtt                                                                                                        <  2:1051616/150‑1 (MQ=255)
                                              tGCTGTGATGGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTa                                                                                                       <  1:1591970/150‑1 (MQ=255)
                                               gCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCATGCAGCACTGCTTAAATTTCTTAc                                                                                                      >  1:1646140/1‑150 (MQ=255)
                                                cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                                     >  1:616744/1‑150 (MQ=255)
                                                cTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAcc                                                                                                     <  2:1704549/150‑1 (MQ=255)
                                                   tgATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACt                                                                                                  >  1:690460/1‑150 (MQ=255)
                                                      ttGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTAcc                                                                                               <  2:32389/150‑1 (MQ=255)
                                                       tGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCg                                                                                              <  1:20755/150‑1 (MQ=255)
                                                           gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACg                                                                                          >  1:579675/1‑150 (MQ=255)
                                                           gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACg                                                                                          >  1:2016886/1‑150 (MQ=255)
                                                              cAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGc                                                                                       >  2:1614463/1‑150 (MQ=255)
                                                               aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCATTTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCa                                                                                      >  2:975296/1‑150 (MQ=255)
                                                                aTTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCaa                                                                                     >  2:184987/1‑150 (MQ=255)
                                                                    ttGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt                                                                                 >  2:541985/1‑150 (MQ=255)
                                                                           cGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCATGGATCGTTACg                                                                          >  1:499519/1‑150 (MQ=255)
                                                                             gCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGcc                                                                        >  1:905956/1‑150 (MQ=255)
                                                                                   ttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGtt                                                                  <  1:460117/150‑1 (MQ=255)
                                                                                   ttgttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGtt                                                                  <  1:279884/150‑1 (MQ=255)
                                                                                     gttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc                                                                <  2:1709846/150‑1 (MQ=255)
                                                                                     gttACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACACAATTTGGCCTCTTAATGCAGTCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc                                                                <  2:812595/150‑1 (MQ=255)
                                                                                        aCCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct                                                             >  2:723204/1‑150 (MQ=255)
                                                                                         ccAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctt                                                            >  1:827642/1‑150 (MQ=255)
                                                                                          cAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCtctta                                                           >  1:116581/1‑149 (MQ=255)
                                                                                            gtaGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCAGTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTg                                                         <  2:271496/148‑1 (MQ=255)
                                                                                               gTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCTGCtt                                                      <  2:692156/150‑1 (MQ=255)
                                                                                                tGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCttt                                                     >  1:779340/1‑150 (MQ=255)
                                                                                                   ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt                                                  >  2:1615818/1‑150 (MQ=255)
                                                                                                   ggTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAAGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTAACACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTATCTTCTGCTTTGAt                                                  >  1:1499889/1‑150 (MQ=255)
                                                                                                      gATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCgg                                               >  1:640368/1‑150 (MQ=255)
                                                                                                         cAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTg                                            <  1:788877/150‑1 (MQ=255)
                                                                                                         cAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTg                                            <  1:1771797/150‑1 (MQ=255)
                                                                                                          aGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGc                                           <  1:1963930/150‑1 (MQ=255)
                                                                                                           gATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCt                                          >  2:497159/1‑150 (MQ=255)
                                                                                                            aTAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCTCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg                                         <  2:609475/150‑1 (MQ=255)
                                                                                                            aTAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg                                         <  2:1139098/150‑1 (MQ=255)
                                                                                                               gCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAAc                                      <  2:1078567/150‑1 (MQ=255)
                                                                                                                ccTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACa                                     >  2:578094/1‑150 (MQ=255)
                                                                                                                ccTCAAATTCCTTATTGGGAGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACa                                     <  1:194294/150‑1 (MQ=255)
                                                                                                                  tCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGc                                   >  1:456961/1‑150 (MQ=255)
                                                                                                                      aTTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTACTGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt                               <  1:406261/150‑1 (MQ=255)
                                                                                                                       ggccaaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGTCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTAGATCGGTTGCTTAACAGCttttt                              >  1:304034/7‑150 (MQ=37)
                                                                                                                        tCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTc                             >  1:1786768/1‑150 (MQ=255)
                                                                                                                           aaaTTGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTg                          >  2:459465/3‑150 (MQ=255)
                                                                                                                            tcttGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGc                         <  1:1407563/148‑1 (MQ=255)
                                                                                                                             aTTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCg                        <  1:1836558/150‑1 (MQ=255)
                                                                                                                              ttGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCgg                       <  1:483230/150‑1 (MQ=255)
                                                                                                                               tGGGTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGa                      >  2:1611795/1‑150 (MQ=255)
                                                                                                                               tGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTTAATTTCTTACCACTTCCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGCACAGCTTTTTCCTGCGGa                      >  1:251283/1‑150 (MQ=255)
                                                                                                                               tGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGAggt                      >  2:149886/1‑149 (MQ=255)
                                                                                                                                 ggTGTCAGCGTTAATTCTGGCACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATg                    >  2:535784/1‑150 (MQ=255)
                                                                                                                                   tGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcg                  >  1:1975968/1‑150 (MQ=255)
                                                                                                                                    gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATgcgc                 >  2:786019/1‑150 (MQ=255)
                                                                                                                                     ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGTGcc                >  2:583783/1‑150 (MQ=255)
                                                                                                                                     ccAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGcc                >  2:1943484/1‑150 (MQ=255)
                                                                                                                                     ccAGAAATAACGCCGACGCCCAATAGGGCCTCCTAATGCACGCAGCACTGCTTAACTTTCATAACACTACCGCACGGGCAAGGAGCGTTACGCCCCGGTTTCTCTTCAGCTTTGATCGGTAGCTGAACAGCTTTTTCCTGCGGATGCGcc                <  1:294665/150‑1 (MQ=255)
                                                                                                                                                 cTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCa    >  2:1822670/1‑150 (MQ=255)
                                                                                                                                                   gACACCCAATTTGGCCTCTTAATGCATGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATg  <  2:1646140/150‑1 (MQ=255)
                                                                                                                                                   gACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTCCCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATg  >  1:1875326/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTGCGGATGCGCCATCCAGTACGCATG  >  NC_000913/1990789‑1991085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: