BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,992,083	G→C	5.9%	noncoding (35/76 nt)	glyW ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,992,083	0	G	C	5.9%	449.0 / 15.9	141	noncoding (35/76 nt)	glyW	tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base G (56/75); new base C (6/2); total (63/78)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 149 total aligned reads displayed.

GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCACC  >  NC_000913/1991934‑1992232

gAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGATACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                       >  2:789903/1‑150 (MQ=255)

gAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                       >  1:940851/1‑150 (MQ=255)

     ggACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAgg                                                                                                                                                  <  2:1565895/150‑1 (MQ=255)

        cTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTGTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCg                                                                                                                                               <  1:50722/150‑1 (MQ=255)

          aGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                             >  2:1808557/1‑150 (MQ=255)

           gACGGATTTGCGATCCGCAACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                                                                                            <  1:443079/150‑1 (MQ=255)

           gACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                                                                                            >  2:1481320/1‑150 (MQ=255)

             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTTAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                          >  1:1096990/1‑150 (MQ=255)

             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                          <  2:1274287/150‑1 (MQ=255)

             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                          >  1:1813714/1‑150 (MQ=255)

             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCATTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCACGACCTTGCCAAGGTCggggtc                                                                                                                                          <  2:68443/150‑6 (MQ=255)

             cGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGACAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACATTGGCAAGGTCGTGctc                                                                                                                                          >  1:1848215/1‑150 (MQ=255)

              ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                                                                                         <  1:1379706/150‑1 (MQ=255)

                 tttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAcc                                                                                                                                      <  1:1439059/150‑1 (MQ=255)

                     cAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGATACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                                  >  1:157646/1‑150 (MQ=255)

                       aTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGACAAGGTCGTGCTCTACCAACTGa                                                                                                                                >  1:1800543/1‑150 (MQ=255)

                         ccGCTACATACCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                                                                                              <  2:1423246/150‑1 (MQ=255)

                         ccGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                                                                                              <  2:720584/150‑1 (MQ=255)

                           gCTACATAACCGCTTTGTTAACGCTCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                            <  2:1048758/150‑1 (MQ=255)

                                aTAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                       >  2:1719374/1‑150 (MQ=255)

                                  aaCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCACCTGAGCTATTCCCGc                                                                                                                     >  2:1801495/1‑150 (MQ=255)

                                  aaCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGc                                                                                                                     >  2:110295/1‑150 (MQ=255)

                                    ccGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAt                                                                                                                   <  2:581366/150‑1 (MQ=255)

                                    ccGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAt                                                                                                                   <  2:1676625/150‑1 (MQ=255)

                                    ccGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTCCTCTCCCAACAGAGCTATTCCCGCAt                                                                                                                   >  1:337497/1‑150 (MQ=255)

                                     cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAtt                                                                                                                  >  1:390314/1‑150 (MQ=255)

                                     cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAtt                                                                                                                  >  1:1128796/1‑150 (MQ=255)

                                        tttGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                               <  1:1480109/150‑1 (MQ=255)

                                          tGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcatca                                                                                                             >  2:157985/1‑150 (MQ=255)

                                           tttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaa                                                                                                            <  2:550119/149‑1 (MQ=255)

                                            ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                           <  2:1652978/150‑1 (MQ=255)

                                             tAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGCCTCGACCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGc                                                                                                          >  1:1047376/1‑150 (MQ=255)

                                                cgcgCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAAt                                                                                                       >  1:1567716/1‑150 (MQ=255)

                                                 gcgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAAta                                                                                                      >  2:1936068/1‑149 (MQ=255)

                                                 gcgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATc                                                                                                      >  2:978485/1‑150 (MQ=255)

                                                  cgcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCCTTCATCAAGCAATCa                                                                                                     >  2:1627082/1‑150 (MQ=255)

                                                   gcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAg                                                                                                    >  2:793706/1‑150 (MQ=255)

                                                    ccAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGt                                                                                                   <  2:661588/150‑1 (MQ=255)

                                                     cAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGCTGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGtt                                                                                                  >  1:1200381/1‑150 (MQ=255)

                                                     cAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGtt                                                                                                  <  2:1938168/150‑1 (MQ=255)

                                                           cttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                            >  2:460984/1‑150 (MQ=255)

                                                              cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTg                                                                                         >  2:992845/1‑150 (MQ=255)

                                                                  ccTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAttt                                                                                     >  1:324409/1‑150 (MQ=255)

                                                                    tttCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTtta                                                                                   >  1:1033069/1‑150 (MQ=255)

                                                                    tttCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTtta                                                                                   <  1:1232797/150‑1 (MQ=255)

                                                                     gtttaaaaGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttat                                                                                  >  1:1224666/8‑150 (MQ=21)

                                                                        aGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTtattat                                                                               <  1:917748/150‑1 (MQ=255)

                                                                         gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                              >  1:780519/1‑150 (MQ=255)

                                                                         gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                              >  2:8776/1‑150 (MQ=255)

                                                                         gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                              >  1:1362828/1‑150 (MQ=255)

                                                                          aaaGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGCACTCGCGCCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAAGCACTTGAGTTTATTATCg                                                                             >  2:1258923/3‑150 (MQ=21)

                                                                           cAGACATCCGCTTGACGCCTATGTCTTTTAAACTGGATCGGGAAACGAGACTCGAACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTACCTACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCTCTTGATTTTATTATCGt                                                                            <  2:1305964/150‑1 (MQ=25)

                                                                           cAGACATCCGCGTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGt                                                                            >  1:530067/1‑150 (MQ=255)

                                                                            aGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTc                                                                           <  2:868205/150‑1 (MQ=255)

                                                                            aGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTc                                                                           <  1:1035967/150‑1 (MQ=255)

                                                                             gACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCt                                                                          <  2:38949/150‑1 (MQ=255)

                                                                               cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTgg                                                                        >  1:1387175/1‑150 (MQ=255)

                                                                                ctCCGCTTGACGCCGACGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGCTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGc                                                                       <  2:537133/149‑1 (MQ=255)

                                                                                aTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGc                                                                       <  1:1053033/150‑1 (MQ=255)

                                                                                aTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGc                                                                       <  1:821370/150‑1 (MQ=255)

                                                                                 tCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCa                                                                      <  2:549239/150‑1 (MQ=255)

                                                                                  ccGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATAACTTGATTTTATTATCGTCTGGCaa                                                                     >  2:279315/1‑150 (MQ=255)

                                                                                        gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTg                                                               <  1:373863/150‑1 (MQ=255)

                                                                                          cGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgcc                                                             >  2:2003148/1‑150 (MQ=255)

                                                                                            ccGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccgc                                                           <  2:1128796/150‑1 (MQ=255)

                                                                                              gATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGccgccg                                                         >  1:626431/1‑150 (MQ=255)

                                                                                                tGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGGCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGtt                                                       >  1:1270772/1‑150 (MQ=255)

                                                                                                  tCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCTCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCg                                                     <  2:706794/150‑1 (MQ=255)

                                                                                                  tCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATATTATTATCGTCTGGCAATCAGTGACGCCGTTCg                                                     >  1:1348400/1‑150 (MQ=255)

                                                                                                   cTTTTAAACTGGATCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGa                                                    <  1:558843/150‑1 (MQ=255)

                                                                                                    ttttAAACTGGAGCGGGAAACGAGACTCGATCTCGCGACCCCGACCTTGGCAAGGTCGTGCTGTACCATCTTAGCTATTGCCGCATTCATCATGCAATCAGTTAATCACTTGTTTTTATTATCGTCTGGCAATCAGTTCCGGCTTTCGAt                                                   <  2:1711128/150‑1 (MQ=25)

                                                                                                     tttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATg                                                  >  2:72696/1‑150 (MQ=255)

                                                                                                     tttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGACGTTCGATg                                                  >  1:1485663/1‑150 (MQ=255)

                                                                                                         aaCTGGAGCGGGAAACGAGACGCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGGCTGGCAATCAGTGCCGCCGTTCGCTGCGtt                                              <  2:228793/150‑1 (MQ=255)

                                                                                                            tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCTTTGCa                                           <  1:1035501/150‑1 (MQ=255)

                                                                                                            tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCa                                           <  2:1998010/150‑1 (MQ=255)

                                                                                                                gcgcGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCt                                       <  2:534952/150‑1 (MQ=255)

                                                                                                                  gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                     <  1:110295/150‑1 (MQ=255)

                                                                                                                   ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTAGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAcg                                    >  2:576377/1‑149 (MQ=255)

                                                                                                                    gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACtt                                   >  2:1973171/1‑150 (MQ=255)

                                                                                                                        cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCt                               >  1:1626351/1‑150 (MQ=255)

                                                                                                                         gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTg                              <  2:1203731/150‑1 (MQ=255)

                                                                                                                             cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcgc                          >  1:1946435/1‑150 (MQ=255)

                                                                                                                             cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcgc                          >  1:1925182/1‑150 (MQ=255)

                                                                                                                                 aaCTCGCGACCACGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATg                      <  2:118870/150‑1 (MQ=255)

                                                                                                                                   cTCGCGACCCCGTCCTTTGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAg                    <  2:1019537/150‑1 (MQ=255)

                                                                                                                                    tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGt                   <  2:1567716/150‑1 (MQ=255)

                                                                                                                                     cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCACATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTATGGCAATCAGTGCCGCCGTTCGATGCGTTGAATTCTACTTACATGGCGCGATGatta                  >  2:1979748/1‑147 (MQ=38)

                                                                                                                                      ggtaCCCCTTCCTTGGCTTTGTCGGGCTCTACCTACTGACCTATTCCCGCATTCATCAAGCAATCAGTTTATCACTTGATTTTATTATCGCCTGGCATTCAGTGCCGCCGTTCGATGCGTTGCATTCTTCTTACCTGGCGCGATGAGTCa                 <  1:714420/147‑1 (MQ=255)

                                                                                                                                         agcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTAGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGAATTCTACTTACCTGGCGCGATGAGTCAACg              >  1:1780138/5‑150 (MQ=255)

                                                                                                                                          gcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa             >  1:776969/4‑150 (MQ=255)

                                                                                                                                           cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGATAGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGat            >  1:795131/3‑150 (MQ=255)

                                                                                                                                             cGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGatat          <  1:460984/150‑1 (MQ=255)

                                                                                                                                                  ttGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTc     <  1:72696/150‑1 (MQ=255)

                                                                                                                                                   tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTcc    >  1:1140460/1‑149 (MQ=255)

                                                                                                                                                   tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca    <  1:992845/150‑1 (MQ=255)

                                                                                                                                                    ggCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTcac   >  2:76354/1‑150 (MQ=255)

                                                                                                                                                    ggCAAGGTCGGGCTCTACCCACTGCCCTATTCCCGCATTCATCAAGCAATCAGTTAATCCCTTGCTTTTATTATCGTCTGGCACTCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTcac   <  2:1206257/150‑1 (MQ=255)

                                                                                                                                                    ggCAAGGGCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCATCGATATTTTTcac   <  2:150176/150‑1 (MQ=255)

                                                                                                                                                     gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTcacc  >  2:1388763/1‑150 (MQ=255)

GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCACC  >  NC_000913/1991934‑1992232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`