Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,996,068 T→A 7.0% intergenic (+26/+42) yecF → / ← sdiA DUF2594 family protein/quorum‑sensing transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,0680TA 7.0% 328.6 / 6.0 111intergenic (+26/+42)yecF/sdiADUF2594 family protein/quorum‑sensing transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (39/62);  new base A (5/4);  total (45/66)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGA  >  NC_000913/1995920‑1996216
                                                                                                                                                    |                                                                                                                                                    
cAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAAGCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCTTCAGTAATATAAAACCGGCTGATAGCGTGCCttt                                                                                                                                                     <  2:1482701/150‑1 (MQ=255)
cAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCttt                                                                                                                                                     >  2:305937/1‑150 (MQ=255)
cAAGCTGACGCGGGCCGCGGGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAACACCGGCTGATAGCGTGCCttt                                                                                                                                                     <  2:1325725/150‑1 (MQ=255)
  aGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGCCGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCa                                                                                                                                                   >  1:1416179/1‑150 (MQ=255)
   gCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAg                                                                                                                                                  <  1:1963773/150‑1 (MQ=255)
    cTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGc                                                                                                                                                 <  1:1992651/150‑1 (MQ=255)
    cTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGc                                                                                                                                                 <  2:330125/150‑1 (MQ=255)
      gACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCg                                                                                                                                               <  2:662526/150‑1 (MQ=255)
        cgcgGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGAGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCAACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTGTCAGCCggg                                                                                                                                             >  1:1654267/1‑149 (MQ=255)
          cgGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGttt                                                                                                                                           <  1:1048744/150‑1 (MQ=255)
           gggCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGtttt                                                                                                                                          <  1:625671/150‑1 (MQ=255)
           gggCCGAGTGATGTTAAAGATTGATAAAGAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCTGTATTTTCCAAAACGGTGAAGCAAATTAACCAAGCCTACCGTCTGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGtttt                                                                                                                                          <  1:1211153/150‑1 (MQ=255)
            ggCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCGGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGAAAGGCACGCTATCAGCCGGtttta                                                                                                                                         <  2:1671391/150‑2 (MQ=38)
             gCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTg                                                                                                                                        <  1:1405690/150‑1 (MQ=255)
              ccGCGGGTTGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCCGGCTGCAGTATTTTCCAAAACGGTTAACCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGc                                                                                                                                       <  1:724247/150‑1 (MQ=255)
                gcgTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCAt                                                                                                                                     <  1:654562/150‑1 (MQ=255)
                gcgTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGAAAGGCACGCTATCAGCCGGttttatatt                                                                                                                                     <  1:1762572/150‑6 (MQ=38)
                  gTGATGTTAAAGAGGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGAAAGGCACGCTATCAGCCGGttttatattac                                                                                                                                   <  2:1589134/150‑8 (MQ=37)
                   tGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTg                                                                                                                                  <  1:1872643/150‑1 (MQ=255)
                    gATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTgg                                                                                                                                 >  2:1410266/1‑150 (MQ=255)
                      tGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCa                                                                                                                               >  1:2008311/1‑150 (MQ=255)
                        ttAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACg                                                                                                                             >  1:482440/1‑150 (MQ=255)
                         tAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAACCGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCCGACGGTTGTTGCATCTGGCACGc                                                                                                                            >  2:1224506/1‑150 (MQ=255)
                         tAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGc                                                                                                                            >  1:6466/1‑150 (MQ=255)
                           aaGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAg                                                                                                                          <  1:313053/150‑1 (MQ=255)
                           aaGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCGGCAGTATTTTCCAAATCGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTATCTGCCGGTGTTTGCATCTgggccgaag                                                                                                                          >  2:104720/1‑143 (MQ=255)
                            aGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAgg                                                                                                                         <  1:301734/150‑1 (MQ=255)
                               tGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACa                                                                                                                      <  1:1942965/150‑1 (MQ=255)
                                ggAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTGTTGCATCTGGCACGCAGGACAg                                                                                                                     >  1:368856/1‑150 (MQ=255)
                                 gAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTAGCATCTGGCACGCAGGACAGa                                                                                                                    >  2:1668626/1‑150 (MQ=255)
                                 gAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCATGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGa                                                                                                                    >  1:1133206/1‑150 (MQ=255)
                                     aaCAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGTCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAag                                                                                                                >  1:1828116/1‑150 (MQ=255)
                                     aaCAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAag                                                                                                                <  1:1656130/150‑1 (MQ=255)
                                     aaCAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAag                                                                                                                <  2:1504685/150‑1 (MQ=255)
                                     aaCAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAag                                                                                                                <  2:1636814/150‑1 (MQ=255)
                                     aaCAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAag                                                                                                                <  2:1879310/150‑1 (MQ=255)
                                       cAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAACCAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAagag                                                                                                              >  1:1782820/1‑150 (MQ=255)
                                          cTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATc                                                                                                           <  1:817796/150‑1 (MQ=255)
                                            tGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCaa                                                                                                         >  2:1100846/1‑150 (MQ=255)
                                            ggcgcTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCaa                                                                                                         <  2:1320871/149‑1 (MQ=255)
                                              cgcTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAAt                                                                                                       <  2:324197/150‑1 (MQ=255)
                                              cgcTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAAt                                                                                                       <  1:1475981/150‑1 (MQ=255)
                                                    tCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGcc                                                                                                 <  1:1544648/150‑1 (MQ=255)
                                                     cGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCa                                                                                                >  1:67147/1‑150 (MQ=255)
                                                      gAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAg                                                                                               >  2:1779660/1‑150 (MQ=255)
                                                       aaGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGt                                                                                              <  2:427371/150‑1 (MQ=255)
                                                       aaGACGAAACACAGGCTGCAGTATTTTACAAAACGGTTAAGCATATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGAGTGCATATCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAACCCAGt                                                                                              <  1:109593/150‑1 (MQ=255)
                                                         gACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAg                                                                                            >  1:1696867/1‑150 (MQ=255)
                                                           cGAACCCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTATCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCg                                                                                          >  1:134337/1‑150 (MQ=255)
                                                           cGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCg                                                                                          <  2:414440/150‑1 (MQ=255)
                                                              aaCCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTAAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGcc                                                                                       >  2:1517663/1‑150 (MQ=255)
                                                                 ccAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCcgcg                                                                                    <  1:775127/150‑1 (MQ=255)
                                                                       tGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAg                                                                              >  2:1604240/1‑150 (MQ=255)
                                                                             aTTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAAcc                                                                        >  1:1772175/1‑150 (MQ=255)
                                                                               tttCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTg                                                                      <  2:1632500/150‑1 (MQ=255)
                                                                                 tCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTggg                                                                    <  1:1100846/150‑1 (MQ=255)
                                                                                 tCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTggg                                                                    <  2:1229153/150‑1 (MQ=255)
                                                                                  ccAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGCTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGt                                                                   <  1:1143825/150‑1 (MQ=255)
                                                                                   cAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTc                                                                  <  1:965030/150‑1 (MQ=255)
                                                                                      aaCGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTCATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTa                                                               <  1:166319/150‑1 (MQ=255)
                                                                                        cGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTAtt                                                             >  1:689309/1‑150 (MQ=255)
                                                                                           ttAAGCAAATTAAACAAGCCTACAGTCAGTAATATAAAACCGGCTGATAGCTTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTTAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTgg                                                          <  1:1991135/150‑1 (MQ=255)
                                                                                            tAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGt                                                         <  1:1684561/150‑1 (MQ=255)
                                                                                             aaGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTg                                                        >  1:1861684/1‑150 (MQ=255)
                                                                                             aaGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTg                                                        <  2:674498/150‑1 (MQ=255)
                                                                                              aGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCATTCAGCCGGTTTTTGCATCTGGCACGCAGCACAGAAAAGAGATCAAATAAAGCCATTACCGGCCGCCTAACACGCAACCTGGTTCTTATTAGGtta                                                       >  1:334673/1‑148 (MQ=255)
                                                                                                cAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCAt                                                     >  1:378791/1‑150 (MQ=255)
                                                                                                cAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCAt                                                     >  1:1353159/1‑150 (MQ=255)
                                                                                                 aaaTTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTCAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCAtt                                                    <  1:159640/150‑1 (MQ=255)
                                                                                                 aaaTTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCAtt                                                    >  1:1065077/1‑150 (MQ=255)
                                                                                                  aaTTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTa                                                   >  2:1819767/1‑150 (MQ=255)
                                                                                                         cAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAAtttttt                                            <  1:779296/150‑1 (MQ=255)
                                                                                                           aGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTc                                          <  1:1277036/150‑1 (MQ=255)
                                                                                                           aGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACACGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTc                                          <  1:1135614/150‑1 (MQ=255)
                                                                                                           aGCCTACAGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTc                                          <  1:1300750/150‑1 (MQ=255)
                                                                                                                 ccGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCACCCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATg                                    >  1:1952592/1‑150 (MQ=255)
                                                                                                                 ccGTCAGTAATATAAAACCGGCTGATAGCGGGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACCGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATg                                    <  2:1696128/150‑1 (MQ=255)
                                                                                                                   gTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGtt                                  <  2:1416179/150‑1 (MQ=255)
                                                                                                                   gTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGtt                                  <  1:647350/150‑1 (MQ=255)
                                                                                                                     cAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGtttt                                <  2:433015/150‑1 (MQ=255)
                                                                                                                      aGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCAGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTATCGTCCGCGTAACAGGCAACCTGGTTCGTATTTGGTGCATTAATTTTTTTCTGCATGttttt                               >  2:200700/1‑150 (MQ=255)
                                                                                                                        tAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCt                             <  1:1692864/150‑1 (MQ=255)
                                                                                                                         aaTATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGGAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGGATGTTTTTCtg                            >  1:1723084/1‑150 (MQ=255)
                                                                                                                          atatAACACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtgg                           <  1:1862975/150‑1 (MQ=255)
                                                                                                                           tataAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggt                          >  1:837281/1‑150 (MQ=255)
                                                                                                                            caaaaaCCGGCTGAAAGGCACGCTATCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtg                         >  2:1815712/3‑150 (MQ=38)
                                                                                                                            caaaaaCCGGCTGAAAGGCACGCTATCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtg                         >  1:1288532/3‑150 (MQ=38)
                                                                                                                            ataAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtg                         >  2:1940806/1‑150 (MQ=255)
                                                                                                                            ataAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtg                         <  1:1410266/150‑1 (MQ=255)
                                                                                                                             taAAACCGGCTGTTATCGTGCCTTTCAGCCGGTTTTTGCATCTGGCAAGCAGGACAGAATAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtgg                        <  1:1224506/150‑1 (MQ=255)
                                                                                                                             taAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTCGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCtggtgg                        >  2:1776946/1‑150 (MQ=255)
                                                                                                                                      cTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTTGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGAGCATTAAATTTTTTCTGCATGTTTTTCTGGTGGATATTGAcc               >  1:55011/1‑150 (MQ=255)
                                                                                                                                       tGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCg              >  2:913119/1‑150 (MQ=255)
                                                                                                                                            gCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATtc         <  2:1145376/150‑1 (MQ=255)
                                                                                                                                            gCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATAAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGGCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATtc         <  2:106434/150‑1 (MQ=255)
                                                                                                                                             cGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATtct        <  2:1195977/150‑1 (MQ=255)
                                                                                                                                              gTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGACATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGAGGAAATTGACCGTATtctc       <  2:289215/150‑1 (MQ=255)
                                                                                                                                                gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAATTTGACCGTATTCTCag     >  2:1719577/1‑150 (MQ=255)
                                                                                                                                                gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCag     <  2:1993146/150‑1 (MQ=255)
                                                                                                                                                   tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGGCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAgaga  >  2:1001420/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGA  >  NC_000913/1995920‑1996216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: