Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,032,336 G→C 6.7% intergenic (‑19/+48) dcm ← / ← yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,032,3360GC 6.7% 380.8 / 15.4 120intergenic (‑19/+48)dcm/yedJDNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (59/52);  new base C (2/6);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

Only 100 of 122 total aligned reads displayed.

GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATG  >  NC_000913/2032188‑2032485
                                                                                                                                                    |                                                                                                                                                     
gATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTga                                                                                                                                                      >  2:1419370/1‑150 (MQ=255)
    ctcACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATg                                                                                                                                                  <  2:1141326/150‑1 (MQ=255)
    ctcACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATg                                                                                                                                                  >  2:1710118/1‑150 (MQ=255)
    ctcACCTACACCATTAAGCTGCACCACCAACGTTTTAACATCCTAAATTAGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATg                                                                                                                                                  <  2:273429/150‑1 (MQ=255)
      cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATTTTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                                >  2:319561/1‑150 (MQ=255)
      cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                                >  2:184025/1‑150 (MQ=255)
       aCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGcc                                                                                                                                               <  1:1578268/150‑1 (MQ=255)
       aCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGAcc                                                                                                                                               <  1:758993/150‑1 (MQ=255)
        ccTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGAATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCg                                                                                                                                              >  2:1978274/1‑150 (MQ=255)
         cTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGTCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCgg                                                                                                                                             <  1:1023443/150‑2 (MQ=255)
         cTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCgg                                                                                                                                             <  1:154160/150‑2 (MQ=255)
         cTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCgg                                                                                                                                             <  1:1055302/150‑2 (MQ=255)
          tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggt                                                                                                                                            <  2:447046/150‑3 (MQ=255)
          tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggt                                                                                                                                            <  2:454582/150‑3 (MQ=255)
          tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCAGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGaa                                                                                                                                            >  1:244891/1‑149 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATTTATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                        >  2:1753350/1‑150 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                        <  2:1928998/150‑1 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                        >  1:1203167/1‑150 (MQ=255)
               cATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGtt                                                                                                                                       >  2:201269/1‑150 (MQ=255)
                    aGCTGCGCCACCCACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGGATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGATAACAt                                                                                                                                  <  2:1689154/150‑1 (MQ=255)
                       tGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAAATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAAt                                                                                                                               >  2:468251/1‑150 (MQ=255)
                           ccaccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa                                                                                                                           <  1:1067932/150‑1 (MQ=255)
                           ccaccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACa                                                                                                                           <  1:684276/150‑1 (MQ=255)
                            caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAg                                                                                                                          <  1:1681455/150‑1 (MQ=255)
                               caACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTTCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCa                                                                                                                       >  2:1841069/1‑150 (MQ=255)
                                   gTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTa                                                                                                                   <  1:225183/150‑1 (MQ=255)
                                     tttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAgg                                                                                                                 <  2:1368156/150‑1 (MQ=255)
                                           atcatAAATTTTCAGCAGTTTCTCCATCATTGCCTGTGCGTCATTCCCGGTGCTGTATTAATAGGTTACTTATATATTTTCATTAATAGATTTCAACGGCCATCTTAGATGGCCTACAGGTTAACATAATTACAGCCATTTAGGGCCAcg                                                                                                           <  2:1058280/150‑1 (MQ=255)
                                           atcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCAcg                                                                                                           <  2:38725/150‑1 (MQ=255)
                                               aaaaTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCt                                                                                                       <  2:1555994/149‑1 (MQ=255)
                                                 aaTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTca                                                                                                     <  1:1972258/150‑1 (MQ=255)
                                                    ttGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagcc                                                                                                  <  1:525664/150‑1 (MQ=255)
                                                      gcagcaGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccag                                                                                                <  2:1951957/150‑1 (MQ=255)
                                                       cagcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGAGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGGCAGGTTATCATAATTACAGCCATTTTGGGCCACGCGCTcagccagc                                                                                               >  1:167677/1‑150 (MQ=255)
                                                         gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGTCCACGCGCTCAGCCAGCgg                                                                                             >  2:1705925/1‑150 (MQ=255)
                                                         gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCgg                                                                                             <  2:45093/150‑1 (MQ=255)
                                                           agTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAt                                                                                           >  2:1321832/1‑150 (MQ=255)
                                                             tttCTCCAGCATTGCCTGTGAGTCAATCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGTGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGa                                                                                         <  1:70200/150‑1 (MQ=255)
                                                              ttCTCCAGCATTGCCTTTGCGGCATTCCCGGTGATGTATTAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGTGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGaa                                                                                        <  2:1164957/150‑1 (MQ=255)
                                                               tctcCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGaaa                                                                                       >  1:1904143/1‑150 (MQ=255)
                                                                ctcCAGCATTGCCTGTGCGGCATTCACGGTGCTGTATGAATCGGTTACTGATATATTTTCCAGCATAGATTTCACCGGCCAACTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGTGCCACGCGATCAGCCAGCGGATGaaaa                                                                                      >  1:221817/1‑150 (MQ=255)
                                                                 tcCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAAc                                                                                     >  1:438952/1‑150 (MQ=255)
                                                                   cAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGc                                                                                   >  1:1000192/1‑150 (MQ=255)
                                                                    aGCATTGCCTGTGCGGCATTCCCGGTGCTGTAGGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCa                                                                                  <  1:463957/150‑1 (MQ=255)
                                                                        ttGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTa                                                                              <  2:618989/150‑1 (MQ=255)
                                                                        ttGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTa                                                                              >  2:1382048/1‑150 (MQ=255)
                                                                         tGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAAAATAATTACAGCCATATAGGGCCACGCGCTCAGCCAGAGGATGAAAACGCATCTAt                                                                             >  1:25496/1‑150 (MQ=255)
                                                                             tgtgCGTCATTCCCGGTGCTGTATGAATCGTTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCAcc                                                                         <  1:1802074/150‑1 (MQ=255)
                                                                              gtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCt                                                                        >  1:898929/1‑150 (MQ=255)
                                                                              gtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCt                                                                        <  2:507423/150‑1 (MQ=255)
                                                                              gtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCGCAGCCAGCGGATGAAAACGCATCTATCACCt                                                                        <  2:689655/150‑1 (MQ=255)
                                                                               tgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCtt                                                                       >  2:393110/1‑150 (MQ=255)
                                                                                  ggCATTCCCGGTGCTGTATGAATCTGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                                    <  2:1507709/150‑1 (MQ=255)
                                                                                  ggCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                                    <  2:838332/150‑1 (MQ=255)
                                                                                  ggCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                                    <  1:1670535/150‑1 (MQ=255)
                                                                                    cATTCCCGGTGCTGTATGAATCGGATACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGAt                                                                  <  2:324110/150‑1 (MQ=255)
                                                                                     aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATc                                                                 <  2:2012096/150‑1 (MQ=255)
                                                                                     aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATc                                                                 >  1:71302/1‑150 (MQ=255)
                                                                                      ttCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCg                                                                <  1:1304291/150‑1 (MQ=255)
                                                                                       tCCCGGTGCTGTATTAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCGCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGa                                                               <  1:1201809/150‑1 (MQ=255)
                                                                                              gCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGACGACAGGTTAAAATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGAATCTATCACCTAGTGATCGACACCTTc                                                        >  1:187404/1‑150 (MQ=255)
                                                                                                tGTATGAATCGGTTACTGATATATTTTCCTGCATAGAATTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCAt                                                      <  2:1570029/150‑1 (MQ=255)
                                                                                                     gAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcc                                                 >  1:1684268/1‑150 (MQ=255)
                                                                                                       aTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcccc                                               <  1:1419370/150‑1 (MQ=255)
                                                                                                       aTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcccc                                               <  1:1553649/150‑1 (MQ=255)
                                                                                                       aTCGGTATCTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGACGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcccc                                               <  1:319561/150‑1 (MQ=255)
                                                                                                          ggTTACTGATATATTTTCCTTCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGACACGCGCTCAGCCAGCGGATGATAACGCATCTATAACCTTGTGATCGACACCTTCATTCTCCCCAGc                                            >  2:570725/1‑150 (MQ=255)
                                                                                                                 gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCg                                     <  2:117817/150‑1 (MQ=255)
                                                                                                                 gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCg                                     >  2:32450/1‑150 (MQ=255)
                                                                                                                   tatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGc                                   <  2:837493/150‑1 (MQ=255)
                                                                                                                   tatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGc                                   >  1:1578361/1‑150 (MQ=255)
                                                                                                                     tatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCAc                                 <  1:1919557/150‑1 (MQ=255)
                                                                                                                       ttttCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTg                               <  1:410908/150‑1 (MQ=255)
                                                                                                                         ttCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACGTGCATTCTCCCCCGCCAGTTCGGAACTGAg                             >  2:545332/1‑150 (MQ=255)
                                                                                                                         ttCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAg                             >  1:1366295/1‑150 (MQ=255)
                                                                                                                           ccTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCt                           <  1:1395672/150‑1 (MQ=255)
                                                                                                                            cTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCtt                          >  1:439802/1‑150 (MQ=255)
                                                                                                                             tGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTc                         >  2:1608905/1‑150 (MQ=255)
                                                                                                                              gCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                        <  2:1203167/150‑1 (MQ=255)
                                                                                                                              gCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                        >  1:201702/1‑150 (MQ=255)
                                                                                                                              gCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                        >  2:1701654/1‑150 (MQ=255)
                                                                                                                              gCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                        <  1:803297/150‑1 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGc                       >  1:1242105/1‑150 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGc                       >  2:1416266/1‑150 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGACATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACCACTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGc                       >  1:1435849/1‑150 (MQ=255)
                                                                                                                                aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGcc                      <  1:184025/150‑1 (MQ=255)
                                                                                                                                 tAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACCCCTTCATTCGCCCCCGCCAGTTCGGCACTGCGCTTCGCCa                     >  1:1791898/1‑150 (MQ=255)
                                                                                                                                  aGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt                    >  2:1372725/1‑150 (MQ=255)
                                                                                                                                   gATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATa                   >  1:1614323/1‑150 (MQ=255)
                                                                                                                                    aTTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATaa                  >  2:862917/1‑150 (MQ=255)
                                                                                                                                        cACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTc              >  2:1771169/1‑150 (MQ=255)
                                                                                                                                        cACCGGCCATCTGAGATCGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGCCACCTACATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTc              >  2:1384423/1‑150 (MQ=255)
                                                                                                                                         gtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCGCCACCTTCATTCTCCCCCGCCAGTTCGGCAGTGAGCTTCGCCATAAACTCg             >  2:1742349/3‑150 (MQ=255)
                                                                                                                                          ccGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCGa            >  2:811246/1‑150 (MQ=255)
                                                                                                                                           cGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGAc           <  1:201269/150‑1 (MQ=255)
                                                                                                                                           cGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGAc           >  1:1111386/1‑150 (MQ=255)
                                                                                                                                             gCCAACTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGAGATCGACACCTACAGTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTa         >  1:1252268/1‑150 (MQ=255)
                                                                                                                                              ccATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaa        >  1:39160/1‑150 (MQ=255)
                                                                                                                                                aTCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaa      >  2:1960024/1‑150 (MQ=255)
                                                                                                                                                    gagaTGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGATAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATg  <  1:86586/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATG  >  NC_000913/2032188‑2032485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: