Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,122 A→G 5.1% intergenic (+76/‑70) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1220AG 5.1% 415.3 / 8.2 136intergenic (+76/‑70)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (60/68);  new base G (5/2);  total (66/70)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 139 total aligned reads displayed.

TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCG  >  NC_000913/2089975‑2090271
                                                                                                                                                   |                                                                                                                                                     
tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATACAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                                                                     <  2:1786220/150‑1 (MQ=255)
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                    >  1:263215/1‑150 (MQ=255)
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                    <  1:1675717/150‑1 (MQ=255)
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCAACATCACCATCATCCTGACTAGTATTTCAGGCGATTTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                    >  1:1762663/1‑150 (MQ=255)
  aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATTATCCTGACTAGTCTTTCAGGCGATGTGTGCGGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                                                                                                                   <  1:1090600/150‑1 (MQ=255)
  aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                                                                                                                   >  1:370845/1‑150 (MQ=255)
  aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACAATCATAACCATCATACTGACTAGTCTTTCAGACTATGTGTGCTGGAAGACATTCAGATCTTCCAGTGTTGCATGAACGCATTAGAAAGCCCCAGGAAGATCAc                                                                                                                                                   >  1:1461995/1‑150 (MQ=255)
    cATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                                                                                                                 >  1:516652/1‑150 (MQ=255)
    cATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                                                                                                                 <  1:1508882/150‑1 (MQ=255)
    cATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCAACATCATCCTGACTAGTCTTTAAGGCGATGTGTGCTGGAAGACATTCAGATCTTCAAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                                                                                                                 >  2:1164570/1‑150 (MQ=255)
        cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGCTCACCTTCCg                                                                                                                                             >  1:1520346/1‑150 (MQ=255)
        cacaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                                                                             >  1:956119/1‑150 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                           <  1:1550571/150‑1 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                           <  1:1720332/150‑1 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                           <  2:1698246/150‑1 (MQ=255)
           agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                          <  1:1493826/150‑1 (MQ=255)
           agagACTTATATGACACGCGTTAAATTTAAACACCACCATCATCACCATCAACCTGACTAGTCTTTCATGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACCCATGAGAAAGCCCCCGGAAGATAACCTTCCgcgg                                                                                                                                          >  1:601070/1‑150 (MQ=255)
              gaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                                                                       <  1:661644/150‑1 (MQ=255)
                 ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt                                                                                                                                    <  1:1946890/150‑1 (MQ=255)
                 ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttt                                                                                                                                    <  1:1939142/150‑1 (MQ=255)
                  tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                                                                   <  2:755228/150‑1 (MQ=255)
                  tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGCCttttt                                                                                                                                   >  2:1258409/1‑150 (MQ=255)
                   ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtttttt                                                                                                                                  <  2:1809744/150‑1 (MQ=255)
                       gACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTg                                                                                                                              >  1:634770/1‑150 (MQ=255)
                       gACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCAGACTAGTCTTTCAGGCGATGTGTGCTGGACGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTg                                                                                                                              <  1:1227657/150‑1 (MQ=255)
                           cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGGGGGCGGGAAGACATTCAGATCTTCCAGTGGTGCAGGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGcgcg                                                                                                                          <  1:140659/150‑1 (MQ=255)
                             cgTTCAATATAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGAGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTATTTATTgcgagat                                                                                                                        >  1:223802/1‑146 (MQ=255)
                                    tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGATTTTTTTTTGGCCGGTTGATAAc                                                                                                                 >  1:392215/1‑150 (MQ=255)
                                      tAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGTGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                                                               >  2:761686/1‑150 (MQ=255)
                                        aaCACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                             >  1:1690528/1‑150 (MQ=255)
                                          caccacCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                                                                           <  1:1197423/150‑1 (MQ=255)
                                              accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                       <  2:605675/150‑1 (MQ=255)
                                                   catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                                                                  <  1:1627503/150‑1 (MQ=255)
                                                   catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                                                                  >  1:692872/1‑150 (MQ=255)
                                                        ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGATTAAaga                                                                                             >  1:1753939/1‑150 (MQ=255)
                                                         catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                                                                            <  2:701850/150‑1 (MQ=255)
                                                          atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                                                           <  1:1053452/150‑1 (MQ=255)
                                                          atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                                                           <  2:160467/150‑1 (MQ=255)
                                                          atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                                                           <  2:1992610/150‑1 (MQ=255)
                                                            catcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                                                                         >  2:86443/1‑150 (MQ=255)
                                                             atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAAt                                                                                        <  2:956119/150‑1 (MQ=255)
                                                              tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATa                                                                                       <  2:1961250/150‑1 (MQ=255)
                                                               ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCAAGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGCGGGCTTTTTTATTGCGCGGTTGATAACCGTTCAGACAGGTTGAAAGAGGaagac                                                                                      >  2:1329037/1‑147 (MQ=255)
                                                                 tGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACTTTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACa                                                                                    <  1:1147966/150‑1 (MQ=255)
                                                                  gACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaa                                                                                   <  2:370845/150‑1 (MQ=255)
                                                                    cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaaa                                                                                 <  2:884762/150‑1 (MQ=255)
                                                                     tAGTCTTTCAGGCGATGTGGGCTGGAAGACATTCAGATCTTCCAGTGGTGGATGAACGCATGAGAAAGCCACCGGAAGATCAACTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAt                                                                                <  1:1358913/150‑1 (MQ=255)
                                                                        tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgac                                                                             >  2:837320/1‑150 (MQ=255)
                                                                         cTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                                                                            >  1:65983/1‑150 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                           >  1:1729726/1‑150 (MQ=255)
                                                                            tCAGGCGATGTGTGCTTGAAGACATTCAGATCTTCCAGTGGTGCATTAACGCATGAGAAAGCCCCCGGAAGATCACCTTACGGTGGCTTTTTTATTGCGCGGTTTATAACGGTTCAGACAGGTTTAAAGAGGAATTACAAAATgacagac                                                                         <  2:803850/150‑1 (MQ=255)
                                                                            tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                                                                         <  2:2017788/150‑1 (MQ=255)
                                                                             cAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                                                                        >  1:861709/1‑150 (MQ=255)
                                                                              aGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTCATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaa                                                                       >  1:1358311/1‑150 (MQ=255)
                                                                                gCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                                                                     >  1:1194705/1‑150 (MQ=255)
                                                                                gCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                                                                     >  1:334093/1‑150 (MQ=255)
                                                                                                 aCATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt                                                    <  2:589272/150‑1 (MQ=255)
                                                                                                  cATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGTGGCTTTTTTATTGCGCGGTTGATACCGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                   <  1:1718891/150‑1 (MQ=255)
                                                                                                  cATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa                                                   >  2:1258553/1‑150 (MQ=255)
                                                                                                     tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc                                                <  1:772770/150‑1 (MQ=255)
                                                                                                     tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc                                                <  1:510879/150‑1 (MQ=255)
                                                                                                       aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAg                                              >  2:1642453/1‑150 (MQ=255)
                                                                                                        gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGCCACCACTCGTTTACGCATAGCTATGCAGa                                             >  2:543936/1‑150 (MQ=255)
                                                                                                        gATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGa                                             <  2:263215/150‑1 (MQ=255)
                                                                                                         aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaa                                            >  2:174234/1‑150 (MQ=255)
                                                                                                          tCTTCCAGTGGTGCATGAACGAATGAGAAAGCCCCCGGAAGATGACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATATCTATGCAGaaa                                           <  2:1461995/150‑1 (MQ=255)
                                                                                                           cTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAAt                                          <  2:1287030/150‑1 (MQ=255)
                                                                                                            ttCGAGTGGTGCATGAACTCATGAGAAAGCCACCGGAAGATCACCTTCCGGTGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGcgtaaacg                                         <  1:1164570/150‑8 (MQ=255)
                                                                                                             tCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATcc                                        >  2:604552/1‑150 (MQ=255)
                                                                                                              ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCg                                       >  2:574408/1‑150 (MQ=255)
                                                                                                                aGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGc                                     <  1:1098287/150‑1 (MQ=255)
                                                                                                                  ttgtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTACGGGGGCTTTTTTATTGCGCTGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCg                                   <  1:1261141/148‑1 (MQ=255)
                                                                                                                  tggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGATTACGCATAGCTATGCAGAAATCCGGCCg                                   >  2:1286757/1‑150 (MQ=255)
                                                                                                                   ggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGt                                  >  2:1082909/1‑150 (MQ=255)
                                                                                                                    gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTACCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGtt                                 <  2:392871/150‑1 (MQ=255)
                                                                                                                      gCATGAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTa                               >  1:853120/1‑150 (MQ=255)
                                                                                                                         tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGt                            <  1:1258409/150‑1 (MQ=255)
                                                                                                                         tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGt                            <  1:1355816/150‑1 (MQ=255)
                                                                                                                          gAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtg                           <  2:722701/150‑1 (MQ=255)
                                                                                                                            aCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgat                         <  2:692872/150‑1 (MQ=255)
                                                                                                                             ctcATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAAGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgatg                        <  1:287254/148‑1 (MQ=255)
                                                                                                                               cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGGTTAAAGAGGCATAACAAAATGACAGACAACCGTCGTTTACGCATAGCTATGCAGAACTCCGGCCGTTTAAGTGATGAc                      >  1:1590017/1‑150 (MQ=255)
                                                                                                                                  tagaAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAATATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCa                   <  2:1462170/149‑1 (MQ=255)
                                                                                                                                  aaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCa                   >  2:1950648/4‑150 (MQ=255)
                                                                                                                                   tgaAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAc                  <  2:1650342/149‑1 (MQ=255)
                                                                                                                                      aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAcgcg               <  2:892493/150‑1 (MQ=255)
                                                                                                                                        gCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGaa             >  2:523860/1‑150 (MQ=255)
                                                                                                                                        gCCCCCCGAAGGTGATCTTCCGGGGGCTTTTTTATAGCGCGGTTTATACCGGTTAAGACAGGTTTAAAGAGGAATATCAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGat             >  2:523861/1‑149 (MQ=255)
                                                                                                                                         cccccGTAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTTATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAt            <  1:21489/150‑1 (MQ=255)
                                                                                                                                         cccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAt            >  1:1167427/1‑150 (MQ=255)
                                                                                                                                         cccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAt            >  1:1222426/1‑150 (MQ=255)
                                                                                                                                          ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt           >  1:610826/1‑150 (MQ=255)
                                                                                                                                           cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCTGCCGTTTAAGTGATGACTCACGCGACttt          >  1:264584/1‑149 (MQ=255)
                                                                                                                                            ccGGAAGATCACCTTCCGGGGGCGTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACGCGTTTACGCCTAGCTATGCAGAAATCCGGCCGTTTAAGTGCTGACTCACGCGAATTGc         >  2:1485827/1‑150 (MQ=255)
                                                                                                                                             cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt        <  2:1520346/150‑1 (MQ=255)
                                                                                                                                             cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt        <  2:793913/150‑1 (MQ=255)
                                                                                                                                               gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTATACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTgg      <  2:62268/150‑1 (MQ=255)
                                                                                                                                                cagagCACCTTCCGGGGGCTGTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTGTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTTCGCATTTCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgc     <  1:1233592/149‑1 (MQ=255)
                                                                                                                                                 aGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcg    >  1:2016331/1‑150 (MQ=255)
                                                                                                                                                   aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTGCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcg  <  1:727489/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCG  >  NC_000913/2089975‑2090271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: