Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,849 C→G 5.1% S16S (TCG→TCC) ‡ wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8490CG 5.1% 419.3 / 10.2 132S16S (TCG→TCC) ‡wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (60/64);  new base G (4/3);  total (65/67)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

Only 100 of 134 total aligned reads displayed.

ACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115700‑2115997
                                                                                                                                                     |                                                                                                                                                    
aCCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGcc                                                                                                                                                      <  1:422025/150‑1 (MQ=255)
 cccaTCCGGCAGGCACGACGACTGTTTGCCCGTCGCTGAAGGCTTGTTTAAATGCGGCGATCCCGGCGTGGGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                     <  2:471208/147‑1 (MQ=255)
 ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTTACGCTTTGTCGGGGGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                     >  1:556212/1‑150 (MQ=255)
 ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                     >  1:1898704/1‑150 (MQ=255)
     tCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                 <  2:1388855/150‑1 (MQ=255)
     accGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                 <  2:605799/149‑1 (MQ=255)
        ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              <  1:415453/150‑1 (MQ=255)
        ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGGTGTTGTCGTTAATGTTAACGCGTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              >  1:431741/1‑150 (MQ=255)
        ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              <  2:453316/150‑1 (MQ=255)
        ggcaggcaAGCCGACTGTTTTCCCTTCGCGGAAGGGTTGGTTAAATGATGGGATCCTGTCGTGTGGGTTGTATTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              <  2:1852238/150‑1 (MQ=255)
         gcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcc                                                                                                                                             <  1:1424465/150‑1 (MQ=255)
            ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                          <  1:167393/150‑1 (MQ=255)
             gcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCTGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAACGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcc                                                                                                                                         <  2:667640/150‑2 (MQ=255)
               acgacgACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCGTGCCGTCAgg                                                                                                                                       >  2:551243/1‑150 (MQ=255)
                    gacTGATTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTTGTAGTCGTTACTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGt                                                                                                                                  <  1:1659873/150‑1 (MQ=255)
                     acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGTCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTCACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGTTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 <  1:1779272/150‑1 (MQ=255)
                     acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 >  1:1304485/1‑150 (MQ=255)
                     acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 <  2:990636/150‑1 (MQ=255)
                        gTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGt                                                                                                                              >  2:10743/1‑150 (MQ=255)
                           tGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTGGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCgg                                                                                                                           <  2:1052734/150‑1 (MQ=255)
                              ccTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGgag                                                                                                                        >  2:49712/1‑150 (MQ=255)
                                    cTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGTCGGCAAGCGCCGAGCTTGCCGTCAGGCACGTGCGTCGGGAGAGtatt                                                                                                                  >  2:1495305/1‑150 (MQ=255)
                                    cTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttt                                                                                                                  >  2:1045648/1‑150 (MQ=255)
                                     ggcaGGCTTGTTTAAATGAGGCGATCCAGCCGTGTGGGTTGTCGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                 <  2:1907141/147‑1 (MQ=255)
                                      gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                                >  1:813174/1‑150 (MQ=255)
                                      gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                                <  2:1704149/150‑1 (MQ=255)
                                       aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGGTAACGCTTTTTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCGTGCCGTCAGGCACGTGCGGCGGGATAGTTTTTTa                                                                                                               >  2:296213/1‑150 (MQ=255)
                                         ggCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATTTTAAAGCTTTTTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATTGAGGAAGGCAAGCGCCCAGCTTAACCGCAGGAACCTGCGGCGGGAGAGTTTTTTaaa                                                                                                             >  2:936135/1‑150 (MQ=255)
                                          gcgtGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAt                                                                                                            <  2:723105/147‑1 (MQ=255)
                                           cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                           <  2:550619/150‑1 (MQ=255)
                                              gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                        >  1:1366750/1‑150 (MQ=255)
                                               tttAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATTTTTACGGTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGCCCGTGCGTCGGGAGAGTTTTTTAAATGGGAt                                                                                                       >  2:1267291/1‑150 (MQ=255)
                                                 tAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCCTGc                                                                                                     >  1:149529/1‑150 (MQ=255)
                                                      gAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTATGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCCAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTAAAATGGCATGCGTtcg                                                                                                >  1:26681/1‑149 (MQ=255)
                                                        ggCGTTCCAGTCGTGTGGGTTGTAGTCGGTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                              <  2:750435/150‑1 (MQ=255)
                                                          cGATCCAGTCGTGGGGGGTGTAGTCGTTAATGTTAACGCTTGGTCGGGCGGGAAGCGCGCGGGCGAAAGTGGTATGGAGGAAGTCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                            <  1:186508/150‑1 (MQ=255)
                                                           gATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCctct                                                                                           >  1:34064/1‑150 (MQ=255)
                                                            aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCGTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCCAGCTTGCCGTCAGGCACGTGCGTGGGGAGAGTTTTTTAAATTGCATGCGTTCTCCTCta                                                                                          >  2:934608/1‑150 (MQ=255)
                                                             tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                         <  1:1173629/150‑1 (MQ=255)
                                                             tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTGGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAGGGCAAGCGCCGCGCTTGCCGTCAGGAAAGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCcat                                                                                         >  1:996836/1‑150 (MQ=255)
                                                               cAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATaa                                                                                       >  2:792265/1‑150 (MQ=255)
                                                                aGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCAGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATaaa                                                                                      <  1:828122/150‑1 (MQ=255)
                                                                     tgtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTg                                                                                 <  2:813174/150‑1 (MQ=255)
                                                                       tgGGGTGGAGTCGTTAATGTTACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGACAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1553876/150‑1 (MQ=255)
                                                                       ggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1816330/149‑1 (MQ=255)
                                                                        gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                              <  1:1883010/150‑1 (MQ=255)
                                                                        gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                              >  1:520512/1‑150 (MQ=255)
                                                                            tGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAg                                                                          >  1:1365058/1‑150 (MQ=255)
                                                                              tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGATCACCTCTATAAAGCCTGCAGCAAGCt                                                                        >  2:477506/1‑150 (MQ=255)
                                                                                 tCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGc                                                                     >  2:1449417/1‑150 (MQ=255)
                                                                                  cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    >  2:454623/1‑150 (MQ=255)
                                                                                  cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    <  1:1521077/150‑1 (MQ=255)
                                                                                   gTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGa                                                                   >  2:1126885/1‑150 (MQ=255)
                                                                                   gTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGa                                                                   >  1:566355/1‑150 (MQ=255)
                                                                                    ttAATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                  <  1:723598/150‑1 (MQ=255)
                                                                                         gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                             <  2:111550/150‑1 (MQ=255)
                                                                                         gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAAGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGCGTtctc                                                             >  1:1840217/1‑150 (MQ=255)
                                                                                          ttACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCg                                                            <  1:775703/150‑1 (MQ=255)
                                                                                            aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCACGCTGGCGAGTTCTCGAt                                                          >  1:1465401/1‑150 (MQ=255)
                                                                                              cGCTTTGTCGGGCGGGACGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                        <  2:1264414/150‑1 (MQ=255)
                                                                                               gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                       >  1:1820000/1‑150 (MQ=255)
                                                                                               gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                       <  1:1035005/150‑1 (MQ=255)
                                                                                                 tttGTCGGGCGGGAAGCGCGCGGGCGATAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGTGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                     <  2:1712029/150‑1 (MQ=255)
                                                                                                      cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTGGATTGATCACctg                                                >  2:293063/1‑150 (MQ=255)
                                                                                                      cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCCGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                >  2:1895923/1‑150 (MQ=255)
                                                                                                       gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                               >  1:1879966/1‑150 (MQ=255)
                                                                                                          cgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTAGCCGGCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCAATAACGCCTGCAGCACGCTGGCGAGTTCTCGTTTGAACACCTGCTgg                                            >  1:1278775/1‑150 (MQ=255)
                                                                                                           gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTACCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                           <  1:936135/150‑1 (MQ=255)
                                                                                                            ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                          >  2:1681068/1‑150 (MQ=255)
                                                                                                             gtaGCGCGCGGGCGAAAGTGGTATGGAGGAAGGCAAGCGCCGAGGTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                         <  2:972238/148‑1 (MQ=255)
                                                                                                             gAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                         <  1:691443/150‑1 (MQ=255)
                                                                                                              aaGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaa                                        <  2:1818206/150‑1 (MQ=255)
                                                                                                                  gcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATc                                    <  1:1083322/150‑1 (MQ=255)
                                                                                                                   cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                   >  2:145079/1‑150 (MQ=255)
                                                                                                                   cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                   >  1:1576167/1‑150 (MQ=255)
                                                                                                                     ctggCGAAAGGCGGATGGAGTAAGGAAAGCTCCGAGCATGCCGTCATGAACGTGCGTCGGGAGTGTTTTTTAAATGGCATGCGTTCTCCTCTATACAGCCTGCAGCAAGCTGTCGAGTTCTCGATTGAACACCTGCTGGTTAAAATCgtg                                 <  2:947984/148‑1 (MQ=255)
                                                                                                                        gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                              <  1:627393/150‑1 (MQ=255)
                                                                                                                         cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             <  2:1274257/150‑1 (MQ=255)
                                                                                                                         cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  1:1378782/1‑150 (MQ=255)
                                                                                                                         cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  2:1252814/1‑150 (MQ=255)
                                                                                                                         cGAAAGGGGAATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             <  1:1134943/150‑1 (MQ=255)
                                                                                                                             aGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtt                         >  2:1607093/1‑150 (MQ=255)
                                                                                                                              ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGAATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        <  2:431741/150‑1 (MQ=255)
                                                                                                                               gggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                       <  1:10743/150‑1 (MQ=255)
                                                                                                                                ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCATCttttt                      <  1:1900770/150‑1 (MQ=255)
                                                                                                                                 gTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTc                     <  2:1344553/150‑1 (MQ=255)
                                                                                                                                 gTATGGAGGAAGGCAAGCGCAGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAAGGCATGCGTTCTCCTCTATAAAGCCAGCAGCAAGCTGGCGAGTTCTCGATTCATCACCTCCTGGTTGAAATCGTGTTCAACTTTTTc                     >  1:75242/1‑150 (MQ=255)
                                                                                                                                  tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                    <  2:1377499/150‑1 (MQ=255)
                                                                                                                                   atcgcggaCGGCAAGCGCCGAGCTTGCCGTCAGGTACGTGCGTCGGGAGAGGTTATAAACTGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcac                   <  2:298366/145‑3 (MQ=255)
                                                                                                                                      cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg                >  2:1418251/7‑150 (MQ=255)
                                                                                                                                       ctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  1:2002071/6‑150 (MQ=255)
                                                                                                                                       aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  1:1312875/1‑150 (MQ=255)
                                                                                                                                       aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  2:1619035/1‑150 (MQ=255)
                                                                                                                                            ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCGGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGtt          >  1:143399/1‑150 (MQ=255)
                                                                                                                                             gCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCGCCTGCTGGTTAAAATCTTGTTCAACTTTTTCGCGCGCGCGttt         >  2:1981137/1‑150 (MQ=255)
                                                                                                                                              cacgcgaCGAGCTTGGCGTCAGGCCCGTGCGGCGGGAGAGTTGTGTAACTGGCATGCGTTCTCCTCAATACCGCCTGCCGCACGCGGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGGTTg        <  1:296213/147‑1 (MQ=255)
                                                                                                                                              cAAGCGCCGAGCTTGCCGTCATGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg        <  1:1495305/150‑1 (MQ=255)
                                                                                                                                              cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg        <  2:1405287/150‑1 (MQ=255)
                                                                                                                                                 gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacg     <  1:1126885/150‑1 (MQ=255)
                                                                                                                                                    ccGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAcc  >  2:874463/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115700‑2115997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: