Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,851 A→C 6.8% S16A (TCG→GCG) ‡ wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8510AC 6.8% 371.3 / 9.8 130S16A (TCG→GCG) ‡wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (57/63);  new base C (7/3);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115705‑2115997
                                                                                                                                                  |                                                                                                                                                  
tCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                 <  2:1388855/150‑1 (MQ=255)
tCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                 <  2:613886/150‑1 (MQ=255)
accGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                 <  2:605799/149‑1 (MQ=255)
   ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGGTGTTGTCGTTAATGTTAACGCGTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              >  1:431741/1‑150 (MQ=255)
   ggcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              <  2:453316/150‑1 (MQ=255)
   ggcaggcaAGCCGACTGTTTTCCCTTCGCGGAAGGGTTGGTTAAATGATGGGATCCTGTCGTGTGGGTTGTATTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGc                                                                                                                                              <  2:1852238/150‑1 (MQ=255)
    gcaggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcc                                                                                                                                             <  1:1424465/150‑1 (MQ=255)
       ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                          <  1:167393/150‑1 (MQ=255)
       ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGGAGTCGTTAATGGTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTc                                                                                                                                          <  1:1897979/150‑1 (MQ=255)
        gcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCTGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAACGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcc                                                                                                                                         <  2:667640/150‑2 (MQ=255)
         caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAATTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATTTTAACGCTTTGACGGGAGGGAAGTGCGCGGGAGAAAGGGTTATGGAGGAAAGCAAGCGCCGAGCATGACGTCAg                                                                                                                                        >  1:1241106/1‑150 (MQ=255)
         caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGGTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                        <  1:1484748/150‑1 (MQ=255)
              cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGGAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                   >  1:1405287/1‑150 (MQ=255)
                ccTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 <  2:770233/149‑1 (MQ=255)
                acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGTCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTCACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGTTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 <  1:1779272/150‑1 (MQ=255)
                acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 <  2:990636/150‑1 (MQ=255)
                acTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTg                                                                                                                                 >  1:1304485/1‑150 (MQ=255)
                      tGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTGGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCgg                                                                                                                           <  2:1052734/150‑1 (MQ=255)
                        cccTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGga                                                                                                                         >  2:470785/1‑150 (MQ=255)
                         ccTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGgag                                                                                                                        >  2:49712/1‑150 (MQ=255)
                          cTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCCTAAGGAACGTGCGTCGGgaga                                                                                                                       >  1:406579/1‑150 (MQ=255)
                              gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCTTGCCGGCAGGTACGTGGGTCGGGAGAGttt                                                                                                                   >  2:1056843/1‑150 (MQ=255)
                              gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                   >  1:1333522/1‑150 (MQ=255)
                               cTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttt                                                                                                                  >  1:441072/1‑150 (MQ=255)
                               cTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttt                                                                                                                  >  2:1045648/1‑150 (MQ=255)
                                ggcaGGCTTGTTTAAATGAGGCGATCCAGCCGTGTGGGTTGTCGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                 <  2:1907141/147‑1 (MQ=255)
                                 gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                                <  2:1704149/150‑1 (MQ=255)
                                 gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                                >  1:813174/1‑150 (MQ=255)
                                  aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGGTAACGCTTTTTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCGTGCCGTCAGGCACGTGCGGCGGGATAGTTTTTTa                                                                                                               >  2:296213/1‑150 (MQ=255)
                                  aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                               <  2:1651655/150‑1 (MQ=255)
                                    ggCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATTTTAAAGCTTTTTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATTGAGGAAGGCAAGCGCCCAGCTTAACCGCAGGAACCTGCGGCGGGAGAGTTTTTTaaa                                                                                                             >  2:936135/1‑150 (MQ=255)
                                    ggCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaaa                                                                                                             <  1:1857633/150‑1 (MQ=255)
                                      cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                           <  2:550619/150‑1 (MQ=255)
                                         gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                        >  1:1366750/1‑150 (MQ=255)
                                          tttAAATGAGGCGATCCAGTCGTGTTGGTTGTAGTCCTTAATGTTAACGAGTTGGCGGGCGGGAAGAGCCAGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCCGGAACGTGGGTCGGGGGAGATTTTTATATTGCAt                                                                                                       >  2:1274144/1‑150 (MQ=255)
                                          tttAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATTTTTACGGTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGCCCGTGCGTCGGGAGAGTTTTTTAAATGGGAt                                                                                                       >  2:1267291/1‑150 (MQ=255)
                                          tttAAATGAGGCGATCCAGTCGTGTGGGTTGTAATCGTTAATGTTAAAGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCATGCCGTCAGGAACGGTCGTCGGGAGAGTTTTTTAAATGGcct                                                                                                       >  1:576526/1‑148 (MQ=255)
                                            tAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCCTGc                                                                                                     >  1:149529/1‑150 (MQ=255)
                                                 gAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTATGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCCAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTAAAATGGCATGCGTtcg                                                                                                >  1:26681/1‑149 (MQ=255)
                                                   ggCGTTCCAGTCGTGTGGGTTGTAGTCGGTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                              <  2:750435/150‑1 (MQ=255)
                                                     cGATCCAGTCGTGGGGGGTGTAGTCGTTAATGTTAACGCTTGGTCGGGCGGGAAGCGCGCGGGCGAAAGTGGTATGGAGGAAGTCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                            <  1:186508/150‑1 (MQ=255)
                                                      gATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCctct                                                                                           >  1:34064/1‑150 (MQ=255)
                                                       aTCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCGTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCCAGCTTGCCGTCAGGCACGTGCGTGGGGAGAGTTTTTTAAATTGCATGCGTTCTCCTCta                                                                                          >  2:934608/1‑150 (MQ=255)
                                                        tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtat                                                                                         <  1:1173629/150‑1 (MQ=255)
                                                        tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTGGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAGGGCAAGCGCCGCGCTTGCCGTCAGGAAAGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCcat                                                                                         >  1:996836/1‑150 (MQ=255)
                                                          cAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATaa                                                                                       >  2:792265/1‑150 (MQ=255)
                                                           aGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCAGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATaaa                                                                                      <  1:828122/150‑1 (MQ=255)
                                                                tgtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTg                                                                                 <  2:813174/150‑1 (MQ=255)
                                                                  tgGGGTGGAGTCGTTAATGTTACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGACAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1553876/150‑1 (MQ=255)
                                                                  ggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                               <  2:1816330/149‑1 (MQ=255)
                                                                   gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                              >  1:520512/1‑150 (MQ=255)
                                                                    ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                             >  1:1088745/1‑150 (MQ=255)
                                                                       tGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAg                                                                          >  1:154079/1‑150 (MQ=255)
                                                                            tCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGc                                                                     >  2:1449417/1‑150 (MQ=255)
                                                                             cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    >  2:454623/1‑150 (MQ=255)
                                                                             cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                    <  1:1521077/150‑1 (MQ=255)
                                                                              gTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAATGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGATCGTGCGTCGGGAGAGTTTTTTAAATGTCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGa                                                                   >  1:1690250/1‑150 (MQ=255)
                                                                              gTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGa                                                                   >  2:1126885/1‑150 (MQ=255)
                                                                                    gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                             <  2:111550/150‑1 (MQ=255)
                                                                                     ttACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCg                                                            <  1:775703/150‑1 (MQ=255)
                                                                                       aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCACGCTGGCGAGTTCTCGAt                                                          >  1:1465401/1‑150 (MQ=255)
                                                                                          gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                       >  1:1820000/1‑150 (MQ=255)
                                                                                          gCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGa                                                       <  1:1035005/150‑1 (MQ=255)
                                                                                            tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                     <  2:1502137/150‑1 (MQ=255)
                                                                                                 cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTGGATTGATCACctg                                                >  2:293063/1‑150 (MQ=255)
                                                                                                 cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCCGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                >  2:1895923/1‑150 (MQ=255)
                                                                                                 cgggagggAAGCGCGAGGGCGAAAGTGTTATGGAGGAAGGCAAGCGCCGAGCTTGAAGTCATGAACGTGATTCGGTAGACTTTTTTAAATGTCATTAGTTCTCATCTATAAAGCCTGCAGCAAGCTGGATAGTTCTCGATTTATCACctt                                                >  2:1448341/1‑149 (MQ=255)
                                                                                                  gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                               >  1:1879966/1‑150 (MQ=255)
                                                                                                      gggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTACCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGt                                           <  1:936135/150‑1 (MQ=255)
                                                                                                       ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                          >  2:1681068/1‑150 (MQ=255)
                                                                                                        gtaGCGCGCGGGCGAAAGTGGTATGGAGGAAGGCAAGCGCCGAGGTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                         <  2:972238/148‑1 (MQ=255)
                                                                                                        gAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                         <  1:691443/150‑1 (MQ=255)
                                                                                                         aaGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaa                                        <  2:1818206/150‑1 (MQ=255)
                                                                                                             gcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATc                                    <  1:1083322/150‑1 (MQ=255)
                                                                                                              cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                   >  2:145079/1‑150 (MQ=255)
                                                                                                              cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                   >  1:1576167/1‑150 (MQ=255)
                                                                                                                cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGCTACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                 <  2:954486/150‑1 (MQ=255)
                                                                                                                   gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                              <  2:1383607/150‑1 (MQ=255)
                                                                                                                   gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                              <  1:627393/150‑1 (MQ=255)
                                                                                                                    cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  2:1246424/1‑150 (MQ=255)
                                                                                                                    cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             <  2:1274257/150‑1 (MQ=255)
                                                                                                                    cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  1:1378782/1‑150 (MQ=255)
                                                                                                                    cGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             >  2:1252814/1‑150 (MQ=255)
                                                                                                                    cGAAAGGGGAATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCa                             <  1:1134943/150‑1 (MQ=255)
                                                                                                                        aGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtt                         >  2:1607093/1‑150 (MQ=255)
                                                                                                                         ggggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGAATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttt                        <  2:431741/150‑1 (MQ=255)
                                                                                                                          gggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                       <  1:10743/150‑1 (MQ=255)
                                                                                                                           ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCATCttttt                      <  1:1900770/150‑1 (MQ=255)
                                                                                                                            gTATGGAGGAAGGCAAGCGCAGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAAGGCATGCGTTCTCCTCTATAAAGCCAGCAGCAAGCTGGCGAGTTCTCGATTCATCACCTCCTGGTTGAAATCGTGTTCAACTTTTTc                     >  1:75242/1‑150 (MQ=255)
                                                                                                                             tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                    <  2:1377499/150‑1 (MQ=255)
                                                                                                                             tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                    <  1:740093/150‑1 (MQ=255)
                                                                                                                              atcgcggaCGGCAAGCGCCGAGCTTGCCGTCAGGTACGTGCGTCGGGAGAGGTTATAAACTGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcac                   <  2:298366/145‑3 (MQ=255)
                                                                                                                                 cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg                >  2:1418251/7‑150 (MQ=255)
                                                                                                                                  ctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  1:2002071/6‑150 (MQ=255)
                                                                                                                                  ctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  2:174976/6‑150 (MQ=255)
                                                                                                                                  aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  1:1312875/1‑150 (MQ=255)
                                                                                                                                  aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc               >  2:908284/1‑150 (MQ=255)
                                                                                                                                        gCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCGCCTGCTGGTTAAAATCTTGTTCAACTTTTTCGCGCGCGCGttt         >  2:1981137/1‑150 (MQ=255)
                                                                                                                                         cacgcgaCGAGCTTGGCGTCAGGCCCGTGCGGCGGGAGAGTTGTGTAACTGGCATGCGTTCTCCTCAATACCGCCTGCCGCACGCGGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGGTTg        <  1:296213/147‑1 (MQ=255)
                                                                                                                                              gcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgac   <  2:503453/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACC  >  NC_000913/2115705‑2115997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: