Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,121,586 G→C 5.9% intergenic (‑32/+23) wcaJ ← / ← cpsG colanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,121,5860GC 5.9% 408.6 / 12.6 132intergenic (‑32/+23)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (64/60);  new base C (5/3);  total (69/63)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 133 total aligned reads displayed.

TGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTC  >  NC_000913/2121437‑2121734
                                                                                                                                                     |                                                                                                                                                     
 gACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTGATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGt                                                                                                                                                      >  1:1915702/1‑150 (MQ=255)
    ttCGCAAACCAGCCATAGTCCGTCAAAAATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCTCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                                  <  1:890013/150‑1 (MQ=255)
    ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                                  <  2:199286/150‑1 (MQ=255)
    ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                                  >  2:1453982/1‑150 (MQ=255)
     tCGCATACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTTTTAGCCCCTTACCCGCAGTgggg                                                                                                                                                 <  2:1307861/150‑1 (MQ=255)
     tCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgggg                                                                                                                                                 <  1:1064365/150‑1 (MQ=255)
      cGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCTCAGTGGGGt                                                                                                                                                >  1:1452543/1‑150 (MQ=255)
      cGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGt                                                                                                                                                >  2:1399755/1‑150 (MQ=255)
       gCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTa                                                                                                                                               <  2:1055395/150‑1 (MQ=255)
       gCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTACTGTTATTAGCCCCTTACCCGCAGTGGGGTa                                                                                                                                               >  1:1527800/1‑150 (MQ=255)
        cAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTTGCCCCTTACCCGCAGTGGGGTaa                                                                                                                                              >  2:1660407/1‑150 (MQ=255)
        cAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTaa                                                                                                                                              >  2:708473/1‑150 (MQ=255)
         aaaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCAGAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTGTTTAGATTTGTGATCGTTGTTCCTGTTTTTAGCCCCTTACCCGCAGTGGGGTAAg                                                                                                                                             >  1:981966/1‑150 (MQ=255)
          aaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAgg                                                                                                                                            >  2:1337008/1‑150 (MQ=255)
           aCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAggg                                                                                                                                           <  1:708473/150‑1 (MQ=255)
             cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggggc                                                                                                                                         <  1:725253/150‑3 (MQ=255)
             cAGCCATAGTCCGGCAAACAAGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTGCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggggc                                                                                                                                         <  1:517809/150‑3 (MQ=255)
                  aTAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTACCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATcc                                                                                                                                    <  1:1109888/150‑1 (MQ=255)
                     gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGAc                                                                                                                                 <  1:1315747/150‑1 (MQ=255)
                       ccGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACAt                                                                                                                               >  2:1571298/1‑150 (MQ=255)
                        cGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACAtt                                                                                                                              <  1:1426051/150‑1 (MQ=255)
                         ggCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTa                                                                                                                             >  1:811411/1‑150 (MQ=255)
                           cAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATTCATTGGTTTTCGCTCGCTAGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACt                                                                                                                           <  1:58965/150‑1 (MQ=255)
                            aaaCATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTc                                                                                                                          <  2:149903/150‑1 (MQ=255)
                             aaCATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCg                                                                                                                         <  2:1727214/150‑1 (MQ=255)
                                 tgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                     <  1:1578452/150‑1 (MQ=255)
                                 tgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                     <  2:1960671/150‑1 (MQ=255)
                                 tgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                     >  1:690851/1‑150 (MQ=255)
                                  gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAg                                                                                                                    <  1:1517506/150‑1 (MQ=255)
                                   atgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGc                                                                                                                   <  1:1999709/150‑1 (MQ=255)
                                   atgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGc                                                                                                                   >  1:395430/1‑150 (MQ=255)
                                       tGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACg                                                                                                               >  1:666523/1‑150 (MQ=255)
                                         atatCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTc                                                                                                             <  2:154536/150‑1 (MQ=255)
                                         atatCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTAATAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTc                                                                                                             <  1:1071485/150‑1 (MQ=255)
                                              tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                        <  1:1804263/150‑1 (MQ=255)
                                              tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                        >  1:1972008/1‑150 (MQ=255)
                                              tGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcagcag                                                                                                        <  1:1438361/150‑1 (MQ=255)
                                              tGAAAAGCGTTGCAACATAGAGATTAACGATGCATTGGTTTTCGCTCGCTAGCGCTTTTTTAGATTTGTCATCGTTGTTGCTGTTAATAGCCCCTTACCCGCAGTGGGGTAAGGGAACCTCCGTCATTACTCGTTCAGCTACGTcagcag                                                                                                        >  1:1403660/1‑150 (MQ=255)
                                               gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCaga                                                                                                       >  2:893335/1‑150 (MQ=255)
                                               gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCaga                                                                                                       >  1:997553/1‑150 (MQ=255)
                                               gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGAGCCGACATTACTCGTTCAGCAACGTCAGCaga                                                                                                       >  1:343891/1‑150 (MQ=255)
                                               gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCATTACCCGCAGTGGGGTAAGGGAAGCTCCGACATTACTCGTTCAGCAACGTCAGCaga                                                                                                       >  2:123730/1‑150 (MQ=255)
                                                 aaaGCGTTGCACCATAGAGATTAACGATGCATTGTCTATCGCTCTCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTAATTAGCACCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTAAGCAACGTCAGCAGAgt                                                                                                     <  1:1784602/150‑1 (MQ=255)
                                                  aaGCGTTGCACCATAGATATTAACGATGCATTGGTTATCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgtt                                                                                                    <  1:195023/150‑1 (MQ=255)
                                                    gCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcg                                                                                                  <  1:938619/150‑1 (MQ=255)
                                                    gCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcg                                                                                                  >  2:240199/1‑150 (MQ=255)
                                                     cGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgc                                                                                                 >  1:1225395/1‑150 (MQ=255)
                                                      gTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcg                                                                                                >  1:1311321/1‑150 (MQ=255)
                                                       ttGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGGAACGTCAGCAGAgttcgcgt                                                                                               <  2:1111199/150‑1 (MQ=255)
                                                           aCCCTAGCGATTCACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGAGTTGTCCTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcggtcgc                                                                                           <  2:911360/150‑1 (MQ=255)
                                                           aCCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                           <  2:620120/150‑1 (MQ=255)
                                                              aTAGAGATTAACGATGCATTGGTTTTCGCTCGCGCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCt                                                                                        <  1:1832017/150‑1 (MQ=255)
                                                               tAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCtt                                                                                       >  1:102931/1‑150 (MQ=255)
                                                                agagATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTc                                                                                      <  1:156648/150‑1 (MQ=255)
                                                                      tAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAg                                                                                >  2:1983706/1‑150 (MQ=255)
                                                                          gATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGc                                                                            >  1:1579949/1‑150 (MQ=255)
                                                                              cATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCCGCAGAGTTCGCGTTCGCGCTTCCATACGCGGCACAt                                                                        >  2:888008/1‑150 (MQ=255)
                                                                              cATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAt                                                                        >  2:560646/1‑150 (MQ=255)
                                                                              cATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAt                                                                        >  1:256293/1‑150 (MQ=255)
                                                                               aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                       >  1:321598/1‑150 (MQ=255)
                                                                                 tGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCAc                                                                     <  2:1145606/150‑1 (MQ=255)
                                                                                       tcgctcgctcgcGCTTTTTTAGATTTGGCATCGTTGTTCCTTTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcgcg                                                               <  1:1644270/150‑1 (MQ=255)
                                                                                       tcgcgcgcgcgcGCTTTTTTAGCTTTGTCATCGTTGTTCCTGTTCTTAGCCCCTTCCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCGTCCATCAGCGGCACATCACcgcgcg                                                               <  1:292677/150‑1 (MQ=255)
                                                                                         gctcgctcgcGCTTTTTTAGATTTGTCATCGTGGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTAGCGCTTCCATCAGCGGCCCATCCCcgcgcgct                                                             >  2:300648/1‑148 (MQ=255)
                                                                                            cgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTcc                                                          >  2:1892503/1‑150 (MQ=255)
                                                                                             gctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCca                                                         <  2:690851/150‑1 (MQ=255)
                                                                                              ctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCcac                                                        <  1:1880300/150‑1 (MQ=255)
                                                                                               tcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCcaca                                                       >  2:1122504/1‑150 (MQ=255)
                                                                                               gcgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTGCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCcaca                                                       <  1:639663/149‑1 (MQ=255)
                                                                                                cgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACAt                                                      <  2:665337/150‑1 (MQ=255)
                                                                                                  cgcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTc                                                    >  2:821004/1‑150 (MQ=255)
                                                                                                       ttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGgcg                                               >  2:1643462/1‑150 (MQ=255)
                                                                                                        tttAGATTTGTCATCGTTGTTCCTGTTCTTCGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGgcgc                                              <  2:1008094/150‑1 (MQ=255)
                                                                                                              tttGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCaccacc                                        >  1:144809/1‑150 (MQ=255)
                                                                                                                 gTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGt                                     <  1:1898541/150‑1 (MQ=255)
                                                                                                                   cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCCCCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                   >  2:1656979/1‑150 (MQ=255)
                                                                                                                   cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                   >  1:1028081/1‑150 (MQ=255)
                                                                                                                   cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                   >  1:1202341/1‑150 (MQ=255)
                                                                                                                      cGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGt                                >  2:1126968/1‑150 (MQ=255)
                                                                                                                          gttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGCTATTg                            >  2:757765/1‑150 (MQ=255)
                                                                                                                            tCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGa                          <  1:1337008/150‑1 (MQ=255)
                                                                                                                            tCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGa                          >  2:1028283/1‑150 (MQ=255)
                                                                                                                             ccTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAg                         >  2:1791865/1‑150 (MQ=255)
                                                                                                                              cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                        >  1:1191498/1‑150 (MQ=255)
                                                                                                                              cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                        <  2:811411/150‑1 (MQ=255)
                                                                                                                              cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                        <  2:156128/150‑1 (MQ=255)
                                                                                                                              cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATAACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTGCGGTATTGGAgg                        >  1:1387157/1‑150 (MQ=255)
                                                                                                                               tGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGt                       >  2:714105/1‑150 (MQ=255)
                                                                                                                                    atcttcccTTACCCCACTGCGGGTAAGGGAAGATCCGACTTTACTCGGTCATCAACGTAAGAAGAGTTCGCGTTCGCGCTTACATCAGCGGCACATCAACGCGCGATTCCACATTCAGGCGAACCACCGGTTCGGTAGTGGAGTTGCGCa                  >  2:186383/6‑150 (MQ=255)
                                                                                                                                      cttcccTTACCCCACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg                >  1:510753/4‑150 (MQ=255)
                                                                                                                                       gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt               <  2:144809/150‑1 (MQ=255)
                                                                                                                                       gCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGt               <  1:1098657/150‑1 (MQ=255)
                                                                                                                                        tcccTTACCCCACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt              >  2:1711657/2‑150 (MQ=255)
                                                                                                                                        tcccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt              >  2:1882975/2‑150 (MQ=255)
                                                                                                                                               cccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGTGGTGCGCAGGTTAAAgcgc       >  2:383250/1‑150 (MQ=255)
                                                                                                                                               cccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc       >  1:1048741/1‑150 (MQ=255)
                                                                                                                                               cccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc       <  2:1506728/150‑1 (MQ=255)
                                                                                                                                                ccGCAGTGGTGTAAGGGAAGATCCGACATTACTCGTGCAGCACCGGCAGCAGAGCTCGCGTTCGCGCTTCCATCAGCGTCACATCACCGATCGATTCCACATTCAGGCGCACCACCGGTTCGGTATAGGAGGGGCGCAGGTTAAAGCGcc      <  1:304788/150‑1 (MQ=255)
                                                                                                                                                ccGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGcc      >  1:728606/1‑150 (MQ=255)
                                                                                                                                                    aGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTc  >  2:1050635/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGACTTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTC  >  NC_000913/2121437‑2121734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: