Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,199,607 A→G 8.6% pseudogene (286/948 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,199,6070AG 8.6% 253.0 / 13.1 93pseudogene (286/948 nt)yehHDUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
Reads supporting (aligned to +/- strand):  ref base A (42/42);  new base G (2/6);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

Only 100 of 101 total aligned reads displayed.

CCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACGATGATCTTTTC  >  NC_000913/2199458‑2199756
                                                                                                                                                     |                                                                                                                                                     
ccAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCTATGTGCCTGCGACGCCGCTGGTCGCTGGaaa                                                                                                                                                       >  1:1617267/1‑150 (MQ=255)
ccAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGaaa                                                                                                                                                       <  2:1141363/150‑1 (MQ=255)
ccAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGaaa                                                                                                                                                       <  2:1699649/150‑1 (MQ=255)
  aaaCCGAACAAAAGCGATGATTAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGGTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCTCTGGAAAAc                                                                                                                                                     <  2:361667/150‑1 (MQ=255)
  aaaCCGAACAAAAGCGATGATGAAACGCGGGCGAACCATGCGGTTAACCGCTACAAATTGCTGCAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAAc                                                                                                                                                     <  2:799159/150‑1 (MQ=255)
  aaaCCGAACAAAAGCGATGAGGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAAc                                                                                                                                                     <  1:769912/150‑1 (MQ=255)
     ccGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTc                                                                                                                                                  <  2:1118689/150‑1 (MQ=255)
       gAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCa                                                                                                                                                >  1:794984/1‑150 (MQ=255)
         aCAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGc                                                                                                                                              >  1:1875986/1‑150 (MQ=255)
          cAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAGTTTTCCAGcg                                                                                                                                             <  1:842206/150‑2 (MQ=255)
          cAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGct                                                                                                                                             >  2:1853119/1‑150 (MQ=255)
           aacaGCGATGATGAAACGCGGGCGAACGATGCAGTTAACCGCTACAAATTGCTGAAACAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGGCGCTGGAAGTTTTCCAGcga                                                                                                                                            <  2:1244581/147‑3 (MQ=255)
           aaaaGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGctc                                                                                                                                            >  2:787142/1‑150 (MQ=255)
            aacgCGATGAGGAAACGCGGGCGAACGATGCGGGTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAGTTTTCCAGcgac                                                                                                                                           <  1:546962/147‑4 (MQ=255)
            aaaGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAGTTTTCCAGcgac                                                                                                                                           <  1:1473872/150‑4 (MQ=255)
             aaGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCtt                                                                                                                                          >  1:433638/1‑150 (MQ=255)
               gCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGGACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGGGCCTGCGCCGCCGCTGGTCGCTGGAAGTTTTCCAGcgaccag                                                                                                                                        <  2:1441440/150‑7 (MQ=255)
                cGATGATGAAACGCGGGCGAACGATGCGGGTAACCGCTACAAATTGCTGAAAAAAGATGCGCGGACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCGGCGCCGCCGCTGGTCGCTGGAAGTTTTCCAGcgaccagc                                                                                                                                       <  1:1529171/150‑8 (MQ=255)
                    gatgaAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCGAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGtt                                                                                                                                   <  1:289572/150‑1 (MQ=255)
                     atgaAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTg                                                                                                                                  <  1:1233437/150‑1 (MQ=255)
                       gaAACGCGGGCGAACGATGCTGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAg                                                                                                                                <  1:1006513/150‑1 (MQ=255)
                       gaAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAg                                                                                                                                <  2:1990165/150‑1 (MQ=255)
                          aCGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCAt                                                                                                                             >  2:1321665/1‑150 (MQ=255)
                               ggCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCt                                                                                                                        <  2:1613980/150‑1 (MQ=255)
                                gCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTAGCTGGAAAACTTCCAGCTCGTCCTGGTTGAGCATCCGCTg                                                                                                                       >  2:1563892/1‑150 (MQ=255)
                                 cGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCTGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTgg                                                                                                                      >  2:1858687/1‑150 (MQ=255)
                                 cGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTgg                                                                                                                      <  2:1950630/150‑1 (MQ=255)
                                 cGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTgg                                                                                                                      >  2:1787612/1‑150 (MQ=255)
                                       aTGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTc                                                                                                                >  2:1647234/1‑150 (MQ=255)
                                       aTGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTc                                                                                                                <  2:490162/150‑1 (MQ=255)
                                           ggTTAACCGCTTCAAATTGCTGAAAAAAGAAGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCGATGTGCCTGCGCCGCCGCTGGGCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACtt                                                                                                            <  2:1248507/150‑1 (MQ=255)
                                               aaCCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAAcc                                                                                                        >  1:973736/1‑150 (MQ=255)
                                               aaCCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAAcc                                                                                                        <  1:478837/150‑1 (MQ=255)
                                                 ccGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCACGTCGCTGGAAAACTTCCAGCTCGTCCTGGTTGCGCATCCGCTGGTTCGTCACTTAAccgg                                                                                                      >  1:134113/1‑148 (MQ=255)
                                                     tACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGt                                                                                                  >  1:483015/1‑150 (MQ=255)
                                                             gCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCTCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTACCCCGCCGTCTGATTTg                                                                                          >  2:1511073/1‑150 (MQ=255)
                                                             gCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTg                                                                                          >  1:1815991/1‑150 (MQ=255)
                                                             gCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTg                                                                                          >  1:1152384/1‑150 (MQ=255)
                                                                gAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGGCCCTTAACCCGCCGTCGGCTTTgggg                                                                                       >  1:886398/1‑150 (MQ=255)
                                                                gAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCATGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTgggg                                                                                       <  2:1300337/150‑1 (MQ=255)
                                                                 aaaaaaGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGc                                                                                      >  2:1818622/1‑150 (MQ=255)
                                                                    aaaGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCAGCCATGTGCCTGCTCCGCCGCTGGTCGCTGGAACACTTCCAGCTCTTGCTGGTTGAGCATCCGCTGGTGCGTCACTTAACCCGCCGGCGGAGTTgggggggt                                                                                   >  1:283950/1‑146 (MQ=255)
                                                                      aGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTta                                                                                 <  1:244374/150‑1 (MQ=255)
                                                                         tGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAg                                                                              <  2:1160727/150‑1 (MQ=255)
                                                                              gTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCg                                                                         >  1:1617128/1‑150 (MQ=255)
                                                                                aCCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGaa                                                                       >  1:1570549/1‑150 (MQ=255)
                                                                                      gccgccCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAACCTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACACGCCGTCTGATATGGGGCGTTTATAGCGCCGAAAACCAg                                                                 >  1:99895/1‑150 (MQ=255)
                                                                                      gccgccCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAg                                                                 >  1:592191/1‑150 (MQ=255)
                                                                                      gccgccCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAg                                                                 >  2:1806606/1‑150 (MQ=255)
                                                                                          cccAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTAc                                                             >  1:1856082/1‑150 (MQ=255)
                                                                                          cccAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTAc                                                             >  1:1046079/1‑150 (MQ=255)
                                                                                           ccAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCCCCTCTTCCTGGTTCAGCATCCGCTGGATCGTCACTTAACCCGCCGTCTGAATTGGGGCGTTTATAGCGCCGAAAACCAGCTACt                                                            >  1:1958951/1‑150 (MQ=255)
                                                                                                 ggTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAACACCAGCTACTGgcttg                                                      >  2:599723/1‑150 (MQ=255)
                                                                                                 ggTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGgcttg                                                      <  2:1186133/150‑1 (MQ=255)
                                                                                                  gTAGCAAGTCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGgcttgc                                                     <  2:370847/150‑1 (MQ=255)
                                                                                                    tgCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCATGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGgcttgctt                                                   <  2:415328/149‑1 (MQ=255)
                                                                                                      cAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTc                                                 <  1:1983994/150‑1 (MQ=255)
                                                                                                      cAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTc                                                 >  2:17377/1‑150 (MQ=255)
                                                                                                      cAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTc                                                 >  1:759795/1‑150 (MQ=255)
                                                                                                      cAAGGCGGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCTCTGGAAAACTTCAAGCTCTTGCGGGGTGAGCATCCGCTGGTTAGTCACTTCACCCGACGGCTGAGTTGGGGCTGTTATAGCGAAGCAAACCAGCTACGGGCTTGCTTTc                                                 >  2:119698/1‑150 (MQ=255)
                                                                                                       aaGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcg                                                <  2:1454523/150‑1 (MQ=255)
                                                                                                       aaGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcg                                                <  1:1647385/150‑1 (MQ=255)
                                                                                                       aaGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcg                                                <  1:906099/150‑1 (MQ=255)
                                                                                                       aaGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCGGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGTCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcg                                                <  2:1771433/150‑1 (MQ=255)
                                                                                                         ggCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTTGTTGAGCATCCGCTGGTTCGTCACTTAACGCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcgcg                                              <  2:243320/150‑1 (MQ=255)
                                                                                                         ggCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTcgcg                                              <  1:46208/150‑1 (MQ=255)
                                                                                                           cTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGACAACTTCCAGCTCTTCCTGGTTGAGCCTCCGCTGGTTCGTCACTTAACCCCCCGACTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTa                                            >  1:1043286/1‑150 (MQ=255)
                                                                                                             ggAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGc                                          <  1:1633050/150‑1 (MQ=255)
                                                                                                             ggAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGc                                          >  2:1901222/1‑150 (MQ=255)
                                                                                                             ggAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGGAGc                                          >  1:1846336/1‑150 (MQ=255)
                                                                                                              gAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCa                                         <  1:899515/150‑1 (MQ=255)
                                                                                                                 tCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaa                                      <  1:137356/150‑1 (MQ=255)
                                                                                                                  ccGGCATGTGCCTGCGCCGCCGCTGGGCGCTGGAAAACTTCCAGCTCTGCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaag                                     <  2:835473/150‑1 (MQ=255)
                                                                                                                  ccGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaag                                     <  2:836769/150‑1 (MQ=255)
                                                                                                                  ccGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaag                                     <  2:418112/150‑1 (MQ=255)
                                                                                                                  ccGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaag                                     >  1:2001527/1‑150 (MQ=255)
                                                                                                                   cGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCagaaga                                    >  2:214491/1‑150 (MQ=255)
                                                                                                                    gCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGAt                                   <  2:1555413/150‑1 (MQ=255)
                                                                                                                        tgtgCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACa                               <  2:861027/150‑1 (MQ=255)
                                                                                                                        tgtgCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACa                               <  1:1827169/150‑1 (MQ=255)
                                                                                                                         gtgCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAg                              >  2:1188699/1‑150 (MQ=255)
                                                                                                                         gtgCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAg                              <  1:452592/150‑1 (MQ=255)
                                                                                                                           gCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCt                            >  1:1066582/1‑150 (MQ=255)
                                                                                                                           gCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCt                            <  2:1617267/150‑1 (MQ=255)
                                                                                                                             cTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTcc                          >  1:235027/1‑150 (MQ=255)
                                                                                                                              tGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCa                         <  1:1858687/150‑1 (MQ=255)
                                                                                                                                  ccgccgcTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCAc                     >  1:563864/1‑150 (MQ=255)
                                                                                                                                   cgccgcTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCAcc                    >  2:1161188/1‑150 (MQ=255)
                                                                                                                                      cgcTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCt                 >  2:626354/1‑150 (MQ=255)
                                                                                                                                       aggaagagCTGGAAGTTTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTg                >  2:649832/8‑150 (MQ=255)
                                                                                                                                         tGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGAc              <  1:658897/150‑1 (MQ=255)
                                                                                                                                          ggTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCAAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACg             <  1:139549/150‑1 (MQ=255)
                                                                                                                                           agagCTGGAAGTTTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACga            >  2:1680220/4‑150 (MQ=255)
                                                                                                                                             cGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACgatg          <  1:707493/150‑1 (MQ=255)
                                                                                                                                               cTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGCCgatgat        >  2:386562/1‑150 (MQ=255)
                                                                                                                                                   aaaaCTTCCCGCGCTGCCTGGTTGAGCATCCGCTGGTTAGTCACTTCACCCGCCGTCTGATTGTGGGAGTTTATAGCGCCGAAAACCAGCTACTGTCTTGCTTTCGCGTAGCAGAAGTTAACAGCTCCAGCACCGCTGACGATGATCttt    <  1:329307/150‑1 (MQ=255)
                                                                                                                                                   aaaaCTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCAGAAAACCACCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACGATGATCttt    >  1:809694/1‑150 (MQ=255)
                                                                                                                                                   aaaaCTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGGCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACGATGATCttt    <  1:214491/150‑1 (MQ=255)
                                                                                                                                                    aaaCTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTCGGGCGTTTATAGCGCCGAAAACCAGCAACTGGCTTGCTTTCGCGTAGCAGAAGATAACCGCTACAGCACTGCTGACGATCATCtatt   >  1:1771830/1‑150 (MQ=255)
                                                                                                                                                     aaCTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACGATGATCTTTTc  <  2:1535844/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGCCGCCCAGCAGGTAGCAAGGCTGGAATCCGCCATGTGCCTGCGCCGCCGCTGGTCGCTGGAAAACTTCCAGCTCTTCCTGGTTGAGCATCCGCTGGTTCGTCACTTAACCCGCCGTCTGATTTGGGGCGTTTATAGCGCCGAAAACCAGCTACTGGCTTGCTTTCGCGTAGCAGAAGATAACAGCTCCAGCACCGCTGACGATGATCTTTTC  >  NC_000913/2199458‑2199756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: