Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,553 A→T 6.0% intergenic (‑257/+166) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,5530AT 6.0% 335.1 / 9.2 117intergenic (‑257/+166)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base A (47/63);  new base T (2/5);  total (49/68)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

Only 100 of 122 total aligned reads displayed.

GCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCC  >  NC_000913/2259404‑2259702
                                                                                                                                                     |                                                                                                                                                     
gCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaata                                                                                                                                                       <  1:1747/150‑1 (MQ=255)
    gTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCt                                                                                                                                                   >  2:431259/1‑150 (MQ=255)
    gTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCt                                                                                                                                                   >  2:570406/1‑150 (MQ=255)
      tttACGTAAAGTAAATTACCACCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGc                                                                                                                                                 <  2:305615/150‑1 (MQ=255)
       ttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGcc                                                                                                                                                <  2:1932568/150‑1 (MQ=255)
         aCGTAATGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATTCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCt                                                                                                                                              <  1:1227922/150‑1 (MQ=255)
          cGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCtt                                                                                                                                             >  1:625207/1‑150 (MQ=255)
           gtaaagtaatTTACCGCAAGAGAGGCAAAATGGGCAGATTTGAGCCGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGTAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTg                                                                                                                                            <  2:1660192/150‑1 (MQ=255)
             aaagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCttttt                                                                                                                                          <  2:553649/150‑4 (MQ=255)
              aagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAACTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAgg                                                                                                                                         <  1:1328524/150‑1 (MQ=255)
              aagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAgg                                                                                                                                         >  1:347784/1‑150 (MQ=255)
                gtaaaTTACCGCCCGAGAGGCAAAATCGGCAGATTTGAGCAGGCTCACAAAAAGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCttttttat                                                                                                                                       <  2:1998952/150‑7 (MQ=255)
                gtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCttttttat                                                                                                                                       <  2:803548/150‑7 (MQ=255)
                 taaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTACCCttttttatt                                                                                                                                      <  1:994370/150‑8 (MQ=255)
                       aCCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCt                                                                                                                                <  2:1311939/150‑1 (MQ=255)
                        ccGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATTCGATTAAATGCCTGAATTACATAAGCGAGGAATTTGTGACGGGATTCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                               <  1:1058918/150‑1 (MQ=255)
                        ccGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                               <  1:357841/150‑1 (MQ=255)
                        ccGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                               >  2:1915761/1‑150 (MQ=255)
                        ccGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCAGACCTTGAGGAAATAACCTg                                                                                                                               >  1:272447/1‑150 (MQ=255)
                              gagagGCAAAATGGGCAGATTTGAGCAGGCGCACAAACTGCGAATAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGtt                                                                                                                         <  2:1507381/150‑1 (MQ=255)
                                  ggCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgat                                                                                                                     >  2:239412/1‑150 (MQ=255)
                                  ggCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgat                                                                                                                     >  1:1091067/1‑150 (MQ=255)
                                   gCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgatg                                                                                                                    <  1:1498248/150‑1 (MQ=255)
                                    cAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgatga                                                                                                                   >  1:2001273/1‑150 (MQ=255)
                                     aaaaTGTGCAGATTTGAGCAGGCTCACAAAATGCGAATAAATGCCTGAATAACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATATAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGaa                                                                                                                  <  2:1786058/150‑1 (MQ=255)
                                     aaaaTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGaa                                                                                                                  >  2:1334286/1‑150 (MQ=255)
                                       aaTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACg                                                                                                                <  2:729747/150‑1 (MQ=255)
                                       aaTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACg                                                                                                                <  1:630530/150‑1 (MQ=255)
                                       aaTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCAGAATTACAAAAGCGAGGAATTTGTGACGGGCTGCACATAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACg                                                                                                                <  2:1259877/150‑1 (MQ=255)
                                           ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTTTGACGGGATGCACAGAACAGAACAATAAAAAAGGTCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTg                                                                                                            <  2:66235/150‑1 (MQ=255)
                                           ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTg                                                                                                            <  1:404937/150‑1 (MQ=255)
                                               gATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGt                                                                                                        >  1:1049172/1‑150 (MQ=255)
                                                   tGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCt                                                                                                    <  1:693365/150‑1 (MQ=255)
                                                   tGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGCAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCt                                                                                                    >  2:1623375/1‑150 (MQ=255)
                                                        aGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGa                                                                                               <  2:1759335/150‑1 (MQ=255)
                                                          gCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCATAAAATAATCTGCCCTTGAGGAATTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGaaa                                                                                             <  1:99363/150‑1 (MQ=255)
                                                          gCTCACAAAATGCGATTAAATGCCTGAATTAAAAAAGCGAGGAATTTGTGACGTGATGCACATAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCATTGAGGAAATAACCAGAGTGTTTGATGAACGTTTGAAGTTTCTACTGaaa                                                                                             <  1:235694/150‑1 (MQ=255)
                                                                 aaaTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATaaaa                                                                                      <  2:652371/150‑1 (MQ=255)
                                                                  aaTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAc                                                                                     <  2:347784/150‑1 (MQ=255)
                                                                   aTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAcc                                                                                    <  2:1156712/150‑1 (MQ=255)
                                                                    tGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCa                                                                                   >  1:1207975/1‑150 (MQ=255)
                                                                        aTTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTa                                                                               >  1:1221562/1‑150 (MQ=255)
                                                                         ttAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTaa                                                                              >  1:534912/1‑150 (MQ=255)
                                                                           aaaTTCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGTCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt                                                                            <  1:454384/150‑1 (MQ=255)
                                                                           aaaTGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt                                                                            <  1:461927/150‑1 (MQ=255)
                                                                           aaaTGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt                                                                            >  2:718439/1‑150 (MQ=255)
                                                                            aaTGCCTGTATTACAAAAGCGAGGAATTTGTGACTGGATGCACATAAAATAAAAATAAAAAAGGTCAGAAAATAATCTTCCCTTGAGGAAATAAACTGAGTGTTTGATGAACTTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAtt                                                                           <  1:1799944/150‑1 (MQ=255)
                                                                             aTGCCTGAATTACAAAAGCGAGTAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTc                                                                          <  2:698149/150‑1 (MQ=255)
                                                                              tGCCTGAATTACAAAAGCGAGGAATTTGTGAAGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGGCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCa                                                                         <  2:1679870/150‑1 (MQ=255)
                                                                               gCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCAt                                                                        >  2:1673517/1‑150 (MQ=255)
                                                                                ccTGTATTACAATAGCGATGAATTTGTGACTGGATGAACAGAACAGAACAATTAATAAGGGCTGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                       <  2:1315138/150‑1 (MQ=255)
                                                                                ccTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                       <  2:1315135/150‑1 (MQ=255)
                                                                                ccTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                       <  2:1884746/150‑1 (MQ=255)
                                                                                ccTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTATGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                       >  1:226178/1‑150 (MQ=255)
                                                                                   gAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa                                                                    >  2:902508/1‑150 (MQ=255)
                                                                                   gAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATATAAATTCATaaaa                                                                    >  2:36110/1‑150 (MQ=255)
                                                                                      ttACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTa                                                                 <  1:1344761/150‑1 (MQ=255)
                                                                                              gCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTcaca                                                         >  1:1121523/1‑150 (MQ=255)
                                                                                                gaagAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACaaa                                                       >  1:1301974/4‑150 (MQ=255)
                                                                                                gAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACaaa                                                       >  1:1611938/1‑150 (MQ=255)
                                                                                                 aGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAc                                                      >  2:1431477/1‑150 (MQ=255)
                                                                                                  ggAATTTGTGAGGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAcc                                                     <  2:1000306/150‑1 (MQ=255)
                                                                                                  ggAATTTGTGACGGGATTCACAGAACAGAACAATAAAAAAGGTCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTAGATGAACGTTTGAAGTTTCAACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAcc                                                     <  1:1236668/150‑1 (MQ=255)
                                                                                                   gAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGTGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAcag                                                    >  2:1246598/1‑148 (MQ=255)
                                                                                                     aTTTGTGACGGCATGCACAGAAAAGAACAATAAAAAAGGCCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGACGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAACCCGGt                                                  <  2:1156764/150‑1 (MQ=255)
                                                                                                        tgtgACGGGATGCACAGTACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCAt                                               <  1:1795424/150‑1 (MQ=255)
                                                                                                        tgtgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCAt                                               <  2:1128879/150‑1 (MQ=255)
                                                                                                        tgtgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGGCAt                                               <  1:1128921/150‑1 (MQ=255)
                                                                                                          tgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtc                                             <  2:1271234/150‑1 (MQ=255)
                                                                                                           gACGTGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtct                                            <  2:753798/150‑1 (MQ=255)
                                                                                                           gACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtct                                            >  1:1750694/1‑150 (MQ=255)
                                                                                                              gggATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCtt                                         >  2:1062893/1‑150 (MQ=255)
                                                                                                                 aTGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgc                                      <  2:426243/150‑1 (MQ=255)
                                                                                                                  tGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgcg                                     <  2:610225/150‑1 (MQ=255)
                                                                                                                    cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                                   >  1:1466672/1‑150 (MQ=255)
                                                                                                                    cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                                   >  2:1228864/1‑150 (MQ=255)
                                                                                                                    cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                                   >  1:1097095/1‑150 (MQ=255)
                                                                                                                     acataacataACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtc                                  <  2:1847761/150‑1 (MQ=255)
                                                                                                                      aagaacagaacAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtca                                 <  1:1716385/149‑1 (MQ=255)
                                                                                                                        gaacagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcatc                               >  1:1586976/1‑150 (MQ=255)
                                                                                                                        gaacagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcatc                               >  1:749270/1‑150 (MQ=255)
                                                                                                                         aacagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcatca                              <  1:897786/150‑1 (MQ=255)
                                                                                                                          acagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAACCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCGCATCaa                             >  2:1431180/1‑150 (MQ=255)
                                                                                                                            agaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAAt                           <  1:1372105/150‑1 (MQ=255)
                                                                                                                            agaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAAt                           >  1:155074/1‑150 (MQ=255)
                                                                                                                             gaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAATCCGGTCATTCTCTTTGCGCTCATCAAAtt                          >  1:87716/1‑149 (MQ=255)
                                                                                                                             gaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATg                          <  2:186528/150‑1 (MQ=255)
                                                                                                                                 aaTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTac                      <  2:913759/150‑1 (MQ=255)
                                                                                                                                 aaTAAAAAAGGGCAGAAAATAATCTGACCTTGAGGAATTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTac                      <  2:1208190/150‑1 (MQ=255)
                                                                                                                                  aTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCTCTCATCAAATGTTaca                     >  2:1058120/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacag                    <  2:62184/150‑1 (MQ=255)
                                                                                                                                       caaGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggaca                >  1:1948620/2‑150 (MQ=255)
                                                                                                                                         aGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAAAGTTacaggacagg              >  2:1704369/1‑150 (MQ=255)
                                                                                                                                            gCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGaaa           >  1:227488/1‑150 (MQ=255)
                                                                                                                                             cAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAt          >  2:1435760/1‑150 (MQ=255)
                                                                                                                                                aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc       <  1:2002562/150‑1 (MQ=255)
                                                                                                                                                aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc       <  2:1121523/150‑1 (MQ=255)
                                                                                                                                                  aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTg     <  1:172606/150‑1 (MQ=255)
                                                                                                                                                    taatCTGCACTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCAATTAAATGAATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATCTTACAGGACAGGAAATTTCTGcc   <  1:853957/150‑1 (MQ=255)
                                                                                                                                                     aatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGccc  <  1:431259/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCC  >  NC_000913/2259404‑2259702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: