Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,266,047 T→C 5.2% intergenic (+25/‑198) yeiP → / → yeiQ elongation factor P‑like protein/putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,266,0470TC 5.2% 480.4 / 10.5 156intergenic (+25/‑198)yeiP/yeiQelongation factor P‑like protein/putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (72/75);  new base C (5/3);  total (77/79)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 158 total aligned reads displayed.

CCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATAT  >  NC_000913/2265901‑2266196
                                                                                                                                                  |                                                                                                                                                     
cccGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGc                                                                                                                                                    >  1:470548/1‑150 (MQ=255)
 ccGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGTGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                                                                   >  2:1059892/1‑150 (MQ=255)
   gTAACAAACCGGCGACATTGAGCACTGGGCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCa                                                                                                                                                 <  1:1138434/150‑1 (MQ=255)
      aCAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGc                                                                                                                                              <  2:429767/150‑1 (MQ=255)
            cGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCGTGCAGAAAAGGGATAGCTCAGGCTGTccc                                                                                                                                        >  1:8710/1‑150 (MQ=255)
              gCGACATTGAGCACTGGTCTGGTGATTCAGGTACCATAATACTTAAGCCCGGGCGAAAAAAATCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCTTGCAGAAAAGGGATAGCTCAGGCTGTCCCtt                                                                                                                                      <  2:1605618/150‑1 (MQ=255)
               cGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCttt                                                                                                                                     <  2:1984844/150‑1 (MQ=255)
               cGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGACAGCCTGAGCTATCCCttt                                                                                                                                     <  2:1027239/150‑1 (MQ=255)
                gACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttt                                                                                                                                    >  2:1456375/1‑150 (MQ=255)
                gACATTGAGCACTGGTCTGGTGATTCAGGTACAAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCcttt                                                                                                                                    <  2:120555/150‑1 (MQ=255)
                  cATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttttt                                                                                                                                  >  2:977033/1‑150 (MQ=255)
                  cATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttttt                                                                                                                                  <  2:22660/150‑1 (MQ=255)
                   aTTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAATAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGACAGCCTGAGCTATCCCttttctg                                                                                                                                 <  1:1178669/150‑4 (MQ=255)
                   aTTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                                                                 <  1:230025/150‑1 (MQ=255)
                     tGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                                                                               <  2:1431103/150‑1 (MQ=255)
                     tGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAACCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                                                                               <  1:1675091/150‑1 (MQ=255)
                      gtgCACTGGTCTGGTGTTTCAGGTACCAGAATACTTATGCCGGGGCGAAATAATTCGTATGCATATGGAAGAACGCCGTAATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAtt                                                                                                                              <  1:178736/148‑1 (MQ=255)
                             ggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                                                                       >  1:1099100/1‑150 (MQ=255)
                             ggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATAGAAGATCGCCGTTATATGGGGCGTGCTGACTAACTTCAGACGCATGCAGAAAAGGGATAGCTCAGGATGTCCCTTTTTTAATTTATtata                                                                                                                       >  1:601780/1‑150 (MQ=255)
                              gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                                                                      >  1:642404/1‑150 (MQ=255)
                               tctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAcc                                                                                                                     <  1:1944955/150‑1 (MQ=255)
                               gctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAcc                                                                                                                     <  2:1083986/149‑1 (MQ=255)
                                ctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                                                                                    >  1:1074311/1‑150 (MQ=255)
                                ctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGAGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCATTTTTTAATatattaaagca                                                                                                                    >  2:1214833/1‑145 (MQ=255)
                                 tggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCaa                                                                                                                   <  1:853126/150‑1 (MQ=255)
                                 tggtGATTCAGGTACCAGAAAACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCACTTTTTTAATTTATTATACCaa                                                                                                                   >  2:1738391/1‑150 (MQ=255)
                                  ggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAt                                                                                                                  >  1:897787/1‑150 (MQ=255)
                                   gtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCCTTTTTAATTTATTATACCAAtc                                                                                                                 <  1:300168/150‑1 (MQ=255)
                                    tGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCAGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACAAAtct                                                                                                                >  1:1243509/1‑150 (MQ=255)
                                     gATTCAGGTACCAGAATACTTCAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtctt                                                                                                               <  2:1236758/150‑1 (MQ=255)
                                     gATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtctt                                                                                                               >  1:1007901/1‑150 (MQ=255)
                                       ttCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAACAAtcttct                                                                                                             >  1:1548869/1‑150 (MQ=255)
                                       ttCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAACAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtcttct                                                                                                             >  1:1733443/1‑150 (MQ=255)
                                        tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTa                                                                                                            <  1:555621/150‑1 (MQ=255)
                                          aGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTAtt                                                                                                          >  2:1890585/1‑150 (MQ=255)
                                                 cgAATACTTAAGCCCGCGAGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGCGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCt                                                                                                   <  2:144276/149‑1 (MQ=255)
                                                 aGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCt                                                                                                   <  1:1362287/150‑1 (MQ=255)
                                                    aTACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCCGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTcc                                                                                                <  2:704229/150‑1 (MQ=255)
                                                       cTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                             <  1:704544/150‑1 (MQ=255)
                                                       cTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                             <  1:1197387/150‑1 (MQ=255)
                                                       cTTAAGCCCGGGCGAAAAAATTCGTATCCAAATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                             <  2:1823233/150‑1 (MQ=255)
                                                         tAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAac                                                                                           <  2:214423/150‑1 (MQ=255)
                                                            gCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACAcc                                                                                        >  2:1662821/1‑150 (MQ=255)
                                                               cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTa                                                                                     <  2:787181/150‑1 (MQ=255)
                                                                  gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGc                                                                                  >  1:1861384/1‑150 (MQ=255)
                                                                   cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGcc                                                                                 <  1:1787533/150‑1 (MQ=255)
                                                                    gAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGTCTGTCCCTTTTTTAATTTATTATACCCATATTCTATTTTGCGCTTCATGCACACCTTACGccc                                                                                >  1:1994890/1‑150 (MQ=255)
                                                                    gAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                                                                                <  1:1059892/150‑1 (MQ=255)
                                                                     aaaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGACCACCTTACGCCCt                                                                               >  1:1440545/1‑150 (MQ=255)
                                                                      aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                             >  2:1565506/1‑135 (MQ=255)
                                                                      aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                             <  1:1565506/135‑1 (MQ=255)
                                                                           ttCGTATCCATATCGAAGAACTCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAttt                                                                         <  2:620642/150‑1 (MQ=255)
                                                                             cGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTc                                                                       <  2:1160747/150‑1 (MQ=255)
                                                                             cGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTc                                                                       <  1:439968/150‑1 (MQ=255)
                                                                              gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa                                                                      <  1:646257/150‑1 (MQ=255)
                                                                              gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa                                                                      <  1:954632/150‑1 (MQ=255)
                                                                              gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCATGAACACCTTACGCCCTCAATTTTCa                                                                      <  1:1083800/150‑1 (MQ=255)
                                                                                 tCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTc                                                                   >  2:1305781/1‑150 (MQ=255)
                                                                                  ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACTCCCTCAATTTTCACTCg                                                                  >  2:230465/1‑150 (MQ=255)
                                                                                  ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCg                                                                  <  1:676116/150‑1 (MQ=255)
                                                                                  ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAGCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTGACTCg                                                                  >  2:1078299/1‑150 (MQ=255)
                                                                                     tatCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttg                                                               <  2:585152/150‑1 (MQ=255)
                                                                                       tCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGAATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgat                                                             <  1:216287/150‑1 (MQ=255)
                                                                                        cGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgatt                                                            >  1:728184/1‑150 (MQ=255)
                                                                                         gaagaaCGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgattg                                                           <  2:2591/150‑1 (MQ=255)
                                                                                               cGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATATTCACTCGTTGATTGATCGcc                                                     >  1:840550/1‑150 (MQ=255)
                                                                                               cGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCATTGTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCCCTCGTTGATTGATCGcc                                                     >  1:1314477/1‑150 (MQ=255)
                                                                                                  cGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTc                                                  <  2:455165/150‑1 (MQ=255)
                                                                                                     tataTGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTcaca                                               <  2:1879591/150‑1 (MQ=255)
                                                                                                       tatGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGACCACCTTACGCCCTCAATTTTCACTGGTTGAGTGATCGCCCTCACACt                                             >  2:1364430/1‑150 (MQ=255)
                                                                                                           gggCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCAt                                         <  2:1934483/150‑1 (MQ=255)
                                                                                                              cGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGc                                      <  1:1456375/150‑1 (MQ=255)
                                                                                                              cGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGc                                      >  2:1090248/1‑150 (MQ=255)
                                                                                                               gTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCa                                     <  1:2007047/150‑1 (MQ=255)
                                                                                                                    gACTAACTTCAGCCGCATGCAGAAAATGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTAGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAAc                                <  1:805555/150‑1 (MQ=255)
                                                                                                                        aaCTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACCCTTCATCGCATTAACAATc                            >  1:1158976/1‑150 (MQ=255)
                                                                                                                         aCTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATcc                           <  2:1333574/150‑1 (MQ=255)
                                                                                                                               gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                     >  1:823427/1‑150 (MQ=255)
                                                                                                                               gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                     <  2:1007901/150‑1 (MQ=255)
                                                                                                                               gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                     >  1:52782/1‑150 (MQ=255)
                                                                                                                               gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                     >  1:46627/1‑150 (MQ=255)
                                                                                                                                   aaattaaaaaaGGGACAGCCTGAGCTATCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAAttt                 >  2:1897745/8‑150 (MQ=255)
                                                                                                                                    aattaaaaaaGGGACAGCCTGAGCTATCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTc                >  2:752587/7‑150 (MQ=255)
                                                                                                                                    aTGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTc                <  2:1927217/150‑1 (MQ=255)
                                                                                                                                     tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGCACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCTGCCCAATTTCa               >  2:315103/1‑150 (MQ=255)
                                                                                                                                     tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCa               >  2:1852893/1‑150 (MQ=255)
                                                                                                                                     tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCa               >  2:444415/1‑150 (MQ=255)
                                                                                                                                     tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCa               >  1:1620585/1‑150 (MQ=255)
                                                                                                                                      gCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCaa              >  1:999478/1‑150 (MQ=255)
                                                                                                                                       taaaaaaGGGACAGCCTGAGCTATCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCTATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt             >  2:161490/4‑150 (MQ=255)
                                                                                                                                       cAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt             >  2:206274/1‑150 (MQ=255)
                                                                                                                                       cAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt             <  2:1243509/150‑1 (MQ=255)
                                                                                                                                        aGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAtt            <  2:868027/150‑1 (MQ=255)
                                                                                                                                        aGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATACAGACCAATTTCAAtt            >  1:1705863/1‑150 (MQ=255)
                                                                                                                                         gAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTAGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGAATGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAttt           >  2:1560065/1‑149 (MQ=255)
                                                                                                                                              gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc      <  2:1164946/150‑1 (MQ=255)
                                                                                                                                              gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc      >  2:731660/1‑150 (MQ=255)
                                                                                                                                              gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc      <  1:1081718/150‑1 (MQ=255)
                                                                                                                                                  tAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCTCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCatat  <  1:1018752/150‑1 (MQ=255)
                                                                                                                                                  tAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCatat  <  1:1738391/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATAT  >  NC_000913/2265901‑2266196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: