Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,266,058 G→A 5.0% intergenic (+36/‑187) yeiP → / → yeiQ elongation factor P‑like protein/putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,266,0580GA 5.0% 521.9 / 9.1 159intergenic (+36/‑187)yeiP/yeiQelongation factor P‑like protein/putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (71/80);  new base A (5/3);  total (76/83)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATATAACTTTACAC  >  NC_000913/2265913‑2266206
                                                                                                                                                 |                                                                                                                                                    
cGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCGTGCAGAAAAGGGATAGCTCAGGCTGTccc                                                                                                                                                  >  1:8710/1‑150 (MQ=255)
  gCGACATTGAGCACTGGTCTGGTGATTCAGGTACCATAATACTTAAGCCCGGGCGAAAAAAATCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCTTGCAGAAAAGGGATAGCTCAGGCTGTCCCtt                                                                                                                                                <  2:1605618/150‑1 (MQ=255)
   cGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGACAGCCTGAGCTATCCCttt                                                                                                                                               <  2:1027239/150‑1 (MQ=255)
    gACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttt                                                                                                                                              >  2:1456375/1‑150 (MQ=255)
    gACATTGAGCACTGGTCTGGTGATTCAGGTACAAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCcttt                                                                                                                                              <  2:120555/150‑1 (MQ=255)
     aCATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGACAGCCTGAGCTATCCCttttc                                                                                                                                             <  2:1902729/150‑2 (MQ=255)
      cATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttttt                                                                                                                                            >  2:977033/1‑150 (MQ=255)
       aTTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAATAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGACAGCCTGAGCTATCCCttttctg                                                                                                                                           <  1:1178669/150‑4 (MQ=255)
         tGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                                                                                         <  2:1431103/150‑1 (MQ=255)
         tGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAACCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                                                                                         <  1:1675091/150‑1 (MQ=255)
           aGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAttt                                                                                                                                       <  1:926783/150‑1 (MQ=255)
              aCTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATttatt                                                                                                                                    <  2:5807/150‑1 (MQ=255)
                 ggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATAGAAGATCGCCGTTATATGGGGCGTGCTGACTAACTTCAGACGCATGCAGAAAAGGGATAGCTCAGGATGTCCCTTTTTTAATTTATtata                                                                                                                                 >  1:601780/1‑150 (MQ=255)
                  gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                                                                                >  1:642404/1‑150 (MQ=255)
                   tctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAcc                                                                                                                               <  1:1944955/150‑1 (MQ=255)
                   gctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAcc                                                                                                                               <  2:1083986/149‑1 (MQ=255)
                    ctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                                                                                              >  1:1074311/1‑150 (MQ=255)
                    ctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGAGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCATTTTTTAATatattaaagca                                                                                                                              >  2:1214833/1‑145 (MQ=255)
                      ggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAt                                                                                                                            >  1:1333574/1‑150 (MQ=255)
                       gtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCCTTTTTAATTTATTATACCAAtc                                                                                                                           <  1:300168/150‑1 (MQ=255)
                        tGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCAGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACAAAtct                                                                                                                          >  1:1243509/1‑150 (MQ=255)
                         gATTCAGGTACCAGAATACTTCAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtctt                                                                                                                         <  2:1236758/150‑1 (MQ=255)
                         gATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtctt                                                                                                                         >  1:868027/1‑150 (MQ=255)
                         gATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtctt                                                                                                                         >  1:1007901/1‑150 (MQ=255)
                          aTTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtcttc                                                                                                                        <  1:1602194/150‑1 (MQ=255)
                           ttCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtcttct                                                                                                                       >  1:1096753/1‑150 (MQ=255)
                           ttCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAACAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtcttct                                                                                                                       >  1:1733443/1‑150 (MQ=255)
                                 tACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTg                                                                                                                 >  1:1121726/1‑150 (MQ=255)
                                     cgAATACTTAAGCCCGCGAGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGCGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCt                                                                                                             <  2:144276/149‑1 (MQ=255)
                                     aGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCt                                                                                                             <  1:1362287/150‑1 (MQ=255)
                                        aTACTTAAGCCCGTGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGTTAGCTCAGGCTGTGCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTcc                                                                                                          <  2:691830/150‑1 (MQ=255)
                                        aTACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCCGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTcc                                                                                                          <  2:704229/150‑1 (MQ=255)
                                          aCTTAAGCCCGGGCGAAAAAATTCATATCCAAAACGAAGAACGACGTTATATTGGGCGTCCTCACTAACTTCAGCCGCATGCAGAAAAGGGATACCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCAATTTTGCGCTTACTg                                                                                                        >  2:200636/1‑150 (MQ=255)
                                           cTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                                       <  1:1197387/150‑1 (MQ=255)
                                           cTTAAGCCCGGGCGAAAAAATTCGTATCCAAATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                                       <  2:1823233/150‑1 (MQ=255)
                                                gCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACAcc                                                                                                  >  2:1662821/1‑150 (MQ=255)
                                                   cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTa                                                                                               <  2:787181/150‑1 (MQ=255)
                                                    gggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTAc                                                                                              <  1:599693/150‑1 (MQ=255)
                                                       cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGcc                                                                                           >  2:281263/1‑150 (MQ=255)
                                                       cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGcc                                                                                           >  2:1747852/1‑150 (MQ=255)
                                                       cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGcc                                                                                           <  1:1787533/150‑1 (MQ=255)
                                                        gAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                                                                                          <  1:1059892/150‑1 (MQ=255)
                                                        gAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                                                                                          >  1:1802189/1‑150 (MQ=255)
                                                         aaaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGACCACCTTACGCCCt                                                                                         >  1:1440545/1‑150 (MQ=255)
                                                          aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                                       <  1:1565506/135‑1 (MQ=255)
                                                          aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                                                                                       >  2:1565506/1‑135 (MQ=255)
                                                              aTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtt                                                                                    <  1:1662821/150‑1 (MQ=255)
                                                                tCGTAGCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTAATATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                                                                                  <  2:1982480/150‑1 (MQ=255)
                                                                 cGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTc                                                                                 <  2:1160747/150‑1 (MQ=255)
                                                                  gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa                                                                                <  1:954632/150‑1 (MQ=255)
                                                                  gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa                                                                                <  1:71604/150‑1 (MQ=255)
                                                                  gTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCATGAACACCTTACGCCCTCAATTTTCa                                                                                <  1:1083800/150‑1 (MQ=255)
                                                                     tCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTc                                                                             >  2:1305781/1‑150 (MQ=255)
                                                                     tCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTc                                                                             >  1:220757/1‑150 (MQ=255)
                                                                      ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACTCCCTCAATTTTCACTCg                                                                            >  2:230465/1‑150 (MQ=255)
                                                                      ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCg                                                                            <  1:676116/150‑1 (MQ=255)
                                                                      ccATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAGCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTGACTCg                                                                            >  2:1078299/1‑150 (MQ=255)
                                                                         tatCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttg                                                                         <  2:585152/150‑1 (MQ=255)
                                                                           tCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGAATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgat                                                                       <  1:216287/150‑1 (MQ=255)
                                                                            cGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgatt                                                                      >  1:1891990/1‑150 (MQ=255)
                                                                            cGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgatt                                                                      >  1:728184/1‑150 (MQ=255)
                                                                             gaagaaCGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgattg                                                                     >  1:1710458/1‑150 (MQ=255)
                                                                                   cGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGcc                                                               >  1:41054/1‑150 (MQ=255)
                                                                                   cGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATATTCACTCGTTGATTGATCGcc                                                               >  1:840550/1‑150 (MQ=255)
                                                                                        ttATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTcac                                                          >  2:88050/1‑150 (MQ=255)
                                                                                         tataTGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTcaca                                                         <  2:1879591/150‑1 (MQ=255)
                                                                                           tatGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGACCACCTTACGCCCTCAATTTTCACTGGTTGAGTGATCGCCCTCACACt                                                       >  2:1364430/1‑150 (MQ=255)
                                                                                           tatGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACt                                                       <  1:1742171/150‑1 (MQ=255)
                                                                                           tatGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACt                                                       <  1:460601/150‑1 (MQ=255)
                                                                                              ggggCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCa                                                    <  1:669709/150‑1 (MQ=255)
                                                                                               gggCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCAt                                                   <  2:1934483/150‑1 (MQ=255)
                                                                                                 gCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCg                                                 <  2:765145/150‑1 (MQ=255)
                                                                                                  cGTGCTGACTAACTTCAGCCGCATGCATAAAAGGGATAGCTTATGCTGTCCCTTTTTTAATTTATTATTCCAATCTTCTATTTTGCGCTTCCTGAAAACCTTTCGCCCTCAATTTTCCCTCGTTGATTGATCGCCCTCACACATCATCGc                                                <  1:200636/150‑1 (MQ=255)
                                                                                                  cGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGc                                                >  2:1090248/1‑150 (MQ=255)
                                                                                                  cGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGc                                                <  1:1456375/150‑1 (MQ=255)
                                                                                                        gACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTaaa                                          >  1:814838/1‑149 (MQ=255)
                                                                                                            aaCTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACCCTTCATCGCATTAACAATc                                      >  1:1158976/1‑150 (MQ=255)
                                                                                                            aaCATCAGCCGCATGCAGAAAAGGGAAAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATc                                      <  2:222755/150‑1 (MQ=255)
                                                                                                             aCTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATcc                                     <  2:1333574/150‑1 (MQ=255)
                                                                                                             aCTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATGTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCGCACACTTCATCGCATTACCAATcc                                     >  2:1018263/1‑150 (MQ=255)
                                                                                                                   gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                               >  1:52782/1‑150 (MQ=255)
                                                                                                                   gCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCa                               >  1:823427/1‑150 (MQ=255)
                                                                                                                     cGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCCGACCAAt                             <  2:251024/150‑1 (MQ=255)
                                                                                                                       aaattaaaaaaGGGACAGCCTGAGCTATCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAAttt                           >  2:1897745/8‑150 (MQ=255)
                                                                                                                         tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGCACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCTGCCCAATTTCa                         >  2:315103/1‑150 (MQ=255)
                                                                                                                         tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCa                         >  2:444415/1‑150 (MQ=255)
                                                                                                                         tGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCa                         >  2:1852893/1‑150 (MQ=255)
                                                                                                                          gCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCaa                        >  1:999478/1‑150 (MQ=255)
                                                                                                                           taaaaaaGGGACAGCCTGAGCTATCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCTATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt                       >  2:161490/4‑150 (MQ=255)
                                                                                                                           cAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt                       >  2:206274/1‑150 (MQ=255)
                                                                                                                           cAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAt                       <  2:1243509/150‑1 (MQ=255)
                                                                                                                             gAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTAGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGAATGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAAttt                     >  2:1560065/1‑149 (MQ=255)
                                                                                                                                  gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc                <  2:1164946/150‑1 (MQ=255)
                                                                                                                                  gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc                >  2:731660/1‑150 (MQ=255)
                                                                                                                                  gggATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTc                <  1:1081718/150‑1 (MQ=255)
                                                                                                                                     aTAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCata             >  1:502269/1‑150 (MQ=255)
                                                                                                                                      tAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCTCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCatat            <  1:1018752/150‑1 (MQ=255)
                                                                                                                                        gCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCGTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATATaa          >  2:891933/1‑150 (MQ=255)
                                                                                                                                            aGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATATAACttt      <  2:1887396/150‑1 (MQ=255)
                                                                                                                                                tGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATATAACTTTacac  <  1:1534204/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTCACACTTCATCGCATTAACAATCCAGACCAATTTCAATTGCTGTCATATAACTTTACAC  >  NC_000913/2265913‑2266206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: