Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 191,731 G→A 5.5% intergenic (+23/‑124) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913191,7310GA 5.5% 567.9 / 12.2 182intergenic (+23/‑124)tsf/pyrHtranslation elongation factor EF‑Ts/uridylate kinase
Reads supporting (aligned to +/- strand):  ref base G (104/65);  new base A (3/7);  total (108/74)
Fisher's exact test for biased strand distribution p-value = 9.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 189 total aligned reads displayed.

TGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTA  >  NC_000913/191582‑191880
                                                                                                                                                     |                                                                                                                                                     
tGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAg                                                                                                                                                       <  1:353200/150‑1 (MQ=255)
    cAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGggcg                                                                                                                                                   <  2:2001701/150‑1 (MQ=255)
    cAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGggcg                                                                                                                                                   >  1:1829906/1‑150 (MQ=255)
    cAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGggcg                                                                                                                                                   >  1:1301769/1‑150 (MQ=255)
    cAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGggcg                                                                                                                                                   >  1:1047881/1‑150 (MQ=255)
    cAGCTGCTGAAAGAGCATAACGCTGAAGGGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCTTCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGGCTTAATTATCAAAAAGGAGCCGCCTGAGggcg                                                                                                                                                   <  2:1565017/150‑1 (MQ=255)
      gctgctGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGggcggc                                                                                                                                                 >  1:845278/1‑150 (MQ=255)
          ctgAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGcttct                                                                                                                                             >  1:1377535/1‑150 (MQ=255)
            gAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCttt                                                                                                                                           >  1:1820627/1‑150 (MQ=255)
             aaaGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCCTCAGGCGGCTCCtttt                                                                                                                                          <  1:1543324/150‑1 (MQ=255)
               agagCATAACGCTGAAGTGACTGTCTTCATCCGCTTCGATGTGGGTTAAGGCATCTAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGAGATGTCCAAGCAGTCTTAATTATCAAAAAGAAGCCGCCCTCAGGCGGCTCCTTTTtg                                                                                                                                        <  2:6677/150‑1 (MQ=255)
                  gCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGc                                                                                                                                     >  2:739396/1‑150 (MQ=255)
                   cATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGcc                                                                                                                                    >  1:1175630/1‑150 (MQ=255)
                    aTAACGCTGAAGTGACTGTCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGccc                                                                                                                                   >  1:1150712/1‑150 (MQ=255)
                    aTAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGccc                                                                                                                                   >  1:1816921/1‑150 (MQ=255)
                         gCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCtt                                                                                                                              >  1:274220/1‑150 (MQ=255)
                            gAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTa                                                                                                                           >  2:1829975/1‑150 (MQ=255)
                             aaGTGACTGGCTTTATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTaa                                                                                                                          <  2:1730864/150‑1 (MQ=255)
                                 aaCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTGTGCCCATCTTGTAAATTCa                                                                                                                     >  2:1698756/2‑150 (MQ=255)
                                  aCTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCa                                                                                                                     <  1:502432/150‑1 (MQ=255)
                                   cTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAg                                                                                                                    >  1:173441/1‑150 (MQ=255)
                                   cTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAg                                                                                                                    <  2:575701/150‑1 (MQ=255)
                                    tGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGc                                                                                                                   <  1:523585/150‑1 (MQ=255)
                                         tCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAAcc                                                                                                              <  1:652386/150‑1 (MQ=255)
                                           aTCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCt                                                                                                            >  2:1139409/1‑150 (MQ=255)
                                            tCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTATATGTGCCAATCTTGTAAATTCAGCTAACCCtt                                                                                                           >  1:311004/1‑150 (MQ=255)
                                              cGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTtgt                                                                                                         >  1:106293/1‑150 (MQ=255)
                                                  tCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTgggg                                                                                                     >  1:194247/1‑150 (MQ=255)
                                                     aaGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCCTCTTGTAAATTCAGCTAACCCTTGTGGGGCTg                                                                                                  >  2:239632/1‑150 (MQ=255)
                                                       gTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTgcg                                                                                                <  2:1357740/150‑1 (MQ=255)
                                                        tGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTgcgc                                                                                               <  1:392404/150‑1 (MQ=255)
                                                        tGGCTGCAGGCATCGAGAAAGTTGAGACCGACGTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCATAATTAACAAACAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTATCCCTTGTGGGGCTgcgc                                                                                               <  1:990164/150‑1 (MQ=255)
                                                         gggTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCt                                                                                              <  2:458260/150‑1 (MQ=255)
                                                         gggTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCt                                                                                              >  2:876436/1‑150 (MQ=255)
                                                         gggTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGACCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCt                                                                                              <  1:476574/150‑1 (MQ=255)
                                                          ggTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTg                                                                                             <  2:206042/150‑1 (MQ=255)
                                                             gAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTTATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTTAATTCAGCTAACCCTTGTGGGGCTGCGCTGaaa                                                                                          >  1:209353/1‑150 (MQ=255)
                                                             gAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTACGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGaaa                                                                                          <  1:198992/150‑1 (MQ=255)
                                                              aaGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGaaaa                                                                                         >  2:398861/1‑150 (MQ=255)
                                                                ggCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGc                                                                                       >  1:1573804/1‑150 (MQ=255)
                                                                ggCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGc                                                                                       <  2:1202488/150‑1 (MQ=255)
                                                                ggCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGc                                                                                       >  1:141418/1‑150 (MQ=255)
                                                                cgCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGACGCCTGAGCGCGGCATCTTTTTGTGACCATCTTTTAAAATCAGCTAACCCTTTTGGGGATGCGCTGAAAAGc                                                                                       >  1:1947383/2‑150 (MQ=255)
                                                                  cATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGa                                                                                     >  1:1896052/1‑150 (MQ=255)
                                                                  cATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGa                                                                                     >  1:458274/1‑150 (MQ=255)
                                                                   aTCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGAc                                                                                    >  1:666977/1‑150 (MQ=255)
                                                                     cGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGt                                                                                  >  1:820920/1‑150 (MQ=255)
                                                                     cGAGAAAGTTGAGACTGACTTT‑‑‑GCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACa                                                                               >  2:973998/1‑150 (MQ=255)
                                                                      gagaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTa                                                                                 >  2:231197/1‑150 (MQ=255)
                                                                      gagaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCGTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACACTTGTGGGGCTGGGCTGAAAAGCTACGTa                                                                                 >  2:350157/1‑150 (MQ=255)
                                                                           aGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATg                                                                            >  2:1340312/1‑150 (MQ=255)
                                                                             ttGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTc                                                                          >  2:1232782/1‑150 (MQ=255)
                                                                             ttGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTc                                                                          >  2:913526/1‑150 (MQ=255)
                                                                              ggagaCTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCg                                                                         <  2:1019540/149‑1 (MQ=255)
                                                                                 gagtgactTTGCAGCAGAAGATGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGCAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGAGTGGCTGCGCTGAAAAGCGACGTACAAGGTCGcga                                                                      >  2:291606/4‑148 (MQ=255)
                                                                                 gactgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTa                                                                      >  2:929980/1‑150 (MQ=255)
                                                                                   ctgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGt                                                                    >  2:1637978/1‑150 (MQ=255)
                                                                                     gactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTAt                                                                  <  1:187364/150‑1 (MQ=255)
                                                                                          tGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAAtt                                                             >  1:239260/1‑150 (MQ=255)
                                                                                            cagcagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTTTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCa                                                           <  2:1129049/150‑1 (MQ=255)
                                                                                            cagcagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCa                                                           <  1:1132346/150‑1 (MQ=255)
                                                                                             agcagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCAACTTGTAAATTAAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAt                                                          >  1:1927105/1‑150 (MQ=255)
                                                                                               gataaGTTTCTGGGATTTCCAATCATTCTTATATTTCCAATAGGTTCCGCCTGTTGGCGGCTGCTTTTTGTGCCCATCTTTTATATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAttt                                                        <  1:383602/147‑1 (MQ=255)
                                                                                                        ctgcGATTGCCAATCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTTACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACTATGTCGCTAGTATTAATTCATTTCAATCGTTg                                               <  1:2018633/150‑1 (MQ=255)
                                                                                                        ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTg                                               <  2:1913190/150‑1 (MQ=255)
                                                                                                        ctgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTg                                               <  2:1179148/150‑1 (MQ=255)
                                                                                                        ctgcGATGTACAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTg                                               <  1:724610/150‑1 (MQ=255)
                                                                                                          gcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGAc                                             >  2:606110/1‑150 (MQ=255)
                                                                                                              tGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtc                                         <  1:426722/150‑1 (MQ=255)
                                                                                                                 ccAAGCAGTCTTAATTATAAAAAAGGAGCCGACTGAGGGCGGCTTCTTTTTGTGCCAATCTTGTAAATTCATCTAAACCTTGTGTGGCTGCGCTGAAAAGCGACGTACAATGTCTCTAGTATTAATTCATTTCAATCGATGACACTCTCa                                      >  1:201411/1‑150 (MQ=255)
                                                                                                                  cAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAg                                     <  1:739396/150‑1 (MQ=255)
                                                                                                                        gTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaaga                               >  2:1418258/1‑150 (MQ=255)
                                                                                                                          ctaaaTTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaaa                             >  1:1483169/4‑150 (MQ=255)
                                                                                                                          cTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTAAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTAATTTCAATCGTTGACAGTCTCAGgaaagaaa                             >  1:87140/1‑150 (MQ=255)
                                                                                                                            tAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACa                           >  1:1002789/1‑150 (MQ=255)
                                                                                                                               ttATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATgg                        <  1:1012533/150‑1 (MQ=255)
                                                                                                                                 aTCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGcg                      >  2:1323690/1‑149 (MQ=255)
                                                                                                                                 aTCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGATGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCt                      >  2:700979/1‑150 (MQ=255)
                                                                                                                                  tCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGGACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTa                     >  1:768740/1‑150 (MQ=255)
                                                                                                                                  acAAAAAGAAGCCGCCCTCAGGCGGCTCCTTTTTGTGCCCATATTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATATAATCATTTCAATCGTTGACAGTCTCAGCAAAGAAACATGGCTa                     >  1:1235005/2‑150 (MQ=255)
                                                                                                                                   cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGCAACATGGCTAc                    >  1:1279358/1‑150 (MQ=255)
                                                                                                                                   cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAc                    >  1:632639/1‑150 (MQ=255)
                                                                                                                                   cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAc                    >  2:76421/1‑150 (MQ=255)
                                                                                                                                   cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCAAATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGCAACATGGCTAc                    >  1:8081/1‑150 (MQ=255)
                                                                                                                                    aaaaaGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTAGTAAATTCAGCTAACCCTTGTGGGGCTGCTCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc                   >  1:357731/1‑150 (MQ=255)
                                                                                                                                      aaaGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCaa                 >  2:636315/1‑150 (MQ=255)
                                                                                                                                      aaaGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCGTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCaa                 >  1:939306/1‑150 (MQ=255)
                                                                                                                                        aGGATCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATg               <  2:1116381/150‑1 (MQ=255)
                                                                                                                                         ggAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGc              <  1:1262775/150‑1 (MQ=255)
                                                                                                                                         ggAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGc              >  1:753086/1‑150 (MQ=255)
                                                                                                                                            gccgccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCaaa           <  2:666977/150‑1 (MQ=255)
                                                                                                                                              cgccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAc         >  2:775686/1‑150 (MQ=255)
                                                                                                                                              cgccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAc         <  2:1480584/150‑1 (MQ=255)
                                                                                                                                               gccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGTCAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAcc        >  2:1866872/1‑150 (MQ=255)
                                                                                                                                               gccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAcc        <  1:584827/150‑1 (MQ=255)
                                                                                                                                                 cTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCg      >  1:301598/1‑150 (MQ=255)
                                                                                                                                                  tGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGt     >  1:882407/1‑150 (MQ=255)
                                                                                                                                                  tGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCATTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCTTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGt     >  1:967448/1‑150 (MQ=255)
                                                                                                                                                   gAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTc    <  2:1985255/150‑1 (MQ=255)
                                                                                                                                                    aGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCt   >  1:1618963/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTA  >  NC_000913/191582‑191880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: