Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,898 A→G 5.4% intergenic (+97/+42) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,8980AG 5.4% 402.1 / 8.9 131intergenic (+97/+42)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (61/62);  new base G (2/5);  total (63/68)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

Only 100 of 133 total aligned reads displayed.

AGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTA  >  NC_000913/2282749‑2283046
                                                                                                                                                     |                                                                                                                                                    
aGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATa                                                                                                                                                      <  1:412998/150‑1 (MQ=255)
 gCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa                                                                                                                                                     >  1:1666432/1‑150 (MQ=255)
  cGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAg                                                                                                                                                    <  2:1072009/150‑1 (MQ=255)
       cccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGtt                                                                                                                                               >  1:20703/1‑150 (MQ=255)
       cccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGtt                                                                                                                                               >  2:1761010/1‑150 (MQ=255)
        ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTc                                                                                                                                              <  2:31742/150‑1 (MQ=255)
        ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTc                                                                                                                                              >  2:209844/1‑150 (MQ=255)
         cGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCa                                                                                                                                             <  1:1246344/150‑1 (MQ=255)
          gTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAc                                                                                                                                            >  2:361446/1‑150 (MQ=255)
           tACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGACTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcg                                                                                                                                           >  2:691711/1‑150 (MQ=255)
           tACAAACCGAAGCTGCAGCACATCGACTGCGATCGCGCTAAAATGCTGAATAATTTGTAAAAAACCCCGCTCCGGCGGGTTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcg                                                                                                                                           <  1:1005686/150‑1 (MQ=255)
             cacaCCCAAGCTGCAGCACCTCGTCTTCGTTCGCGCTTAATGGCTGAATAAGTAGTAAAAAACCCCGCGCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                                                                                         <  2:321313/147‑1 (MQ=255)
             cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                                                                                         <  1:1268250/150‑1 (MQ=255)
              aaaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGAAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACTGTATTGCTATTTTTTGCAGGCAAGAAAAGGCGTTCAcgccg                                                                                                                                        >  1:186188/1‑150 (MQ=255)
              aaaCCGAAGCTGCAGCACATCGACTTCGTTCGAGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccg                                                                                                                                        >  2:1024680/1‑150 (MQ=255)
                  cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATc                                                                                                                                    >  2:558925/1‑150 (MQ=255)
                   gAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCt                                                                                                                                   <  2:1303277/150‑1 (MQ=255)
                    aaGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTg                                                                                                                                  <  1:1432331/150‑1 (MQ=255)
                      gCTGCAGCACATCGACTTCGTTCGCGATTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGc                                                                                                                                <  2:1193763/150‑1 (MQ=255)
                          cagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTc                                                                                                                            <  2:1195097/150‑1 (MQ=255)
                          cagcaCATCGACGTCGTTCGCTCTTAATTGCTGAATAAGTTGTAAATAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctgc                                                                                                                            <  1:345835/150‑5 (MQ=255)
                           agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCTGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa                                                                                                                           <  1:1082572/150‑1 (MQ=255)
                           agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa                                                                                                                           <  2:1228572/150‑1 (MQ=255)
                           agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCCATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctgca                                                                                                                           <  1:431386/150‑6 (MQ=255)
                            gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCATTCACGCCGCATCTGGCATTCAg                                                                                                                          >  1:1562713/1‑150 (MQ=255)
                             cacaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGa                                                                                                                         <  1:566680/150‑1 (MQ=255)
                               caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                       <  1:654034/150‑1 (MQ=255)
                               caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                       >  1:1329811/1‑150 (MQ=255)
                               caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                       >  2:345946/1‑150 (MQ=255)
                                aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAaca                                                                                                                      >  2:1543736/1‑150 (MQ=255)
                                 tCGACTTCGGTGGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTTCGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAacac                                                                                                                     <  2:1760336/150‑1 (MQ=255)
                                       tcgttcgCGCTTAATTGCTGATTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                               <  2:233419/150‑1 (MQ=255)
                                       tcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGTGTTTTTTGTATCTGCAGATTATGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                               <  2:369178/150‑1 (MQ=255)
                                       tcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                               >  2:431985/1‑150 (MQ=255)
                                           tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAtt                                                                                                           <  2:1052962/150‑1 (MQ=255)
                                           tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAGTCAGAACACAAAACCGAtt                                                                                                           >  1:1229288/1‑150 (MQ=255)
                                                 ttAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc                                                                                                     >  1:452530/1‑150 (MQ=255)
                                                         tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCGTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                             >  1:1312737/1‑150 (MQ=255)
                                                         tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                             >  2:1386576/1‑150 (MQ=255)
                                                          gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg                                                                                            >  2:1823991/1‑150 (MQ=255)
                                                          gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGCCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg                                                                                            >  2:188955/1‑150 (MQ=255)
                                                            aTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc                                                                                          <  2:1157703/150‑1 (MQ=255)
                                                                gTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgc                                                                                      >  1:539009/1‑150 (MQ=255)
                                                                  tGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcgc                                                                                    >  1:744565/1‑150 (MQ=255)
                                                                   gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAGTATGCCTGATTACGGTATTGCGAGTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCCTTCAGCACACAACACCGAGTAATTGCCGCCAGACGTCCGGCGCt                                                                                   >  1:313106/1‑150 (MQ=255)
                                                                     aaaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc                                                                                 >  2:1960991/1‑150 (MQ=255)
                                                                      aaaaaCCCCGCGCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTGCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa                                                                                <  1:176441/150‑1 (MQ=255)
                                                                          aCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTg                                                                            <  2:692298/150‑1 (MQ=255)
                                                                            cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt                                                                          <  2:403246/150‑1 (MQ=255)
                                                                              cGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcg                                                                        >  1:430434/1‑150 (MQ=255)
                                                                                cTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgcg                                                                      <  1:213631/150‑1 (MQ=255)
                                                                                cTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgcg                                                                      <  2:751537/150‑1 (MQ=255)
                                                                                          gTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                            <  2:1524648/150‑1 (MQ=255)
                                                                                          gTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                            >  2:904909/1‑150 (MQ=255)
                                                                                            tttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggcgg                                                          <  1:1855337/150‑1 (MQ=255)
                                                                                            tttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggcgg                                                          <  2:1699352/150‑1 (MQ=255)
                                                                                             ttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCggcggc                                                         <  2:1713031/150‑1 (MQ=255)
                                                                                               ttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGgcggcgg                                                       >  2:564225/1‑149 (MQ=255)
                                                                                                 gTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGc                                                     >  2:566593/1‑150 (MQ=255)
                                                                                                    tCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCTGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCtt                                                  >  2:1618155/1‑150 (MQ=255)
                                                                                                     cTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCTGCCAGACGTCCGGCGCTGCAATTGGTCGCGAAAATTCGGCGGCGTGCCttt                                                 >  2:1503682/1‑150 (MQ=255)
                                                                                                       gCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTaa                                               <  1:209844/150‑1 (MQ=255)
                                                                                                        cAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAAt                                              <  1:398924/150‑1 (MQ=255)
                                                                                                        cAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAAt                                              <  2:1770934/150‑1 (MQ=255)
                                                                                                         aGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATg                                             <  1:1776759/150‑1 (MQ=255)
                                                                                                          gATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATgg                                            <  1:1927166/150‑1 (MQ=255)
                                                                                                           aTTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGt                                           <  2:1109524/150‑1 (MQ=255)
                                                                                                            ttATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTc                                          <  1:55716/150‑1 (MQ=255)
                                                                                                              aTGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCaa                                        <  1:1024680/150‑1 (MQ=255)
                                                                                                               tGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAACCCGATAAATTGCCGCCAGACGGCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAg                                       <  2:261247/150‑1 (MQ=255)
                                                                                                                gCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGAGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAgg                                      >  2:659750/1‑150 (MQ=255)
                                                                                                                   tGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTAt                                   >  2:1792066/1‑150 (MQ=255)
                                                                                                                    gATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTCGCATTCAGAACACAACACCGATTAATTGCCGCCAGACGACCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGACAAGGTATc                                  >  2:1342687/1‑150 (MQ=255)
                                                                                                                    gATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGGTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTACCTTTAATGGTCAAGGTATc                                  <  1:731431/150‑1 (MQ=255)
                                                                                                                       tACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCTCTTAATTGCCGCCAGACGTCCGGCGATGCAATTGGTCGCGCAAAGTCGGCGGCGTGCCTTTAATGTGCAAGGTATCggg                               >  1:321147/1‑149 (MQ=255)
                                                                                                                         cGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTg                             >  1:630388/1‑150 (MQ=255)
                                                                                                                          ggTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCTGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGc                            <  1:361446/150‑1 (MQ=255)
                                                                                                                            tATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg                          <  1:1548007/150‑1 (MQ=255)
                                                                                                                              ttGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGa                        <  1:345946/150‑1 (MQ=255)
                                                                                                                                gCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATc                      >  1:1735931/1‑150 (MQ=255)
                                                                                                                                 cTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGCCGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATcc                     >  1:1740306/1‑150 (MQ=255)
                                                                                                                                 cTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATcc                     >  1:930290/1‑150 (MQ=255)
                                                                                                                                    ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                  <  2:1103415/150‑1 (MQ=255)
                                                                                                                                    ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCGGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                  <  2:1298861/150‑1 (MQ=255)
                                                                                                                                    tttcttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTAAGAACACACAACCGATTCATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATTGTCAAGGTATCGGTTGCTGGATCCCag                  <  1:108659/150‑1 (MQ=255)
                                                                                                                                     tttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCaga                 >  2:899840/1‑150 (MQ=255)
                                                                                                                                     tttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCaga                 <  2:48711/150‑1 (MQ=255)
                                                                                                                                       tttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCagaag               <  1:63725/150‑1 (MQ=255)
                                                                                                                                        ttGCAGGCCAGATAAGGCGTTCACGCAGCATCTGGAATTCAGAACACAAACCCGATTAATTGCAGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGTATCCAagaaga              >  1:1591152/1‑150 (MQ=255)
                                                                                                                                          gCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATg            <  2:1666432/150‑1 (MQ=255)
                                                                                                                                          gCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATg            >  2:1819995/1‑150 (MQ=255)
                                                                                                                                           cAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGc           >  2:499632/1‑150 (MQ=255)
                                                                                                                                           cAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGc           >  2:74819/1‑150 (MQ=255)
                                                                                                                                            atgCCAGATGCGGCGTGAACGCCTTATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGAGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCTGAAGATGCg          >  1:247635/3‑150 (MQ=255)
                                                                                                                                            aGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCg          <  2:746029/150‑1 (MQ=255)
                                                                                                                                             ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt         >  1:1804455/1‑150 (MQ=255)
                                                                                                                                             ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt         >  2:847968/1‑150 (MQ=255)
                                                                                                                                                 aGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTTCCTCCAGACGTCCTGCGCTTCAATTGGTAGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCAc     <  2:1197980/150‑1 (MQ=255)
                                                                                                                                                  gATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAAGTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCAcc    <  1:413062/150‑1 (MQ=255)
                                                                                                                                                    tAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTa  >  2:1919726/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTA  >  NC_000913/2282749‑2283046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: