Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,913 C→T 6.6% intergenic (+112/+27) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,9130CT 6.6% 381.1 / 10.7 120intergenic (+112/+27)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (56/56);  new base T (3/5);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 126 total aligned reads displayed.

AACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATC  >  NC_000913/2282764‑2283059
                                                                                                                                                     |                                                                                                                                                  
aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCCGGGTTTTTTGTATCTGCAGATTATCCCTGATAACTGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTAAcgccgc                                                                                                                                                    >  2:1728877/1‑150 (MQ=255)
   cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATc                                                                                                                                                 >  2:558925/1‑150 (MQ=255)
    gAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCt                                                                                                                                                <  2:1303277/150‑1 (MQ=255)
     aaGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTg                                                                                                                                               <  1:1432331/150‑1 (MQ=255)
       gCTGCAGCACATCGACTTCGTTCGCGATTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGc                                                                                                                                             <  2:1193763/150‑1 (MQ=255)
           cagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTc                                                                                                                                         <  2:1195097/150‑1 (MQ=255)
           cagcaCATCGACGTCGTTCGCTCTTAATTGCTGAATAAGTTGTAAATAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctgc                                                                                                                                         <  1:345835/150‑5 (MQ=255)
            agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCTGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa                                                                                                                                        <  1:1082572/150‑1 (MQ=255)
            agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCa                                                                                                                                        <  2:1228572/150‑1 (MQ=255)
             gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCATTCACGCCGCATCTGGCATTCAg                                                                                                                                       >  1:1562713/1‑150 (MQ=255)
              cacaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGa                                                                                                                                      <  1:566680/150‑1 (MQ=255)
                caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATTCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                    >  1:48711/1‑150 (MQ=255)
                caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                    <  2:1772853/150‑1 (MQ=255)
                caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                    >  1:1329811/1‑150 (MQ=255)
                caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                                                                                                    >  2:345946/1‑150 (MQ=255)
                 aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAaca                                                                                                                                   >  2:1543736/1‑150 (MQ=255)
                  tCGACTTCGGTGGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTTCGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAacac                                                                                                                                  <  2:1760336/150‑1 (MQ=255)
                        tcgttcgCGCTTAATTGCTGATTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                                            <  2:233419/150‑1 (MQ=255)
                        tcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGTGTTTTTTGTATCTGCAGATTATGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                                            <  2:369178/150‑1 (MQ=255)
                        tcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                                                                                            >  2:431985/1‑150 (MQ=255)
                            tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAtt                                                                                                                        <  2:1052962/150‑1 (MQ=255)
                            tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAtt                                                                                                                        >  1:62720/1‑150 (MQ=255)
                                  ttAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc                                                                                                                  >  1:452530/1‑150 (MQ=255)
                                    tatagCTGTATTAGTTGTAAAAAAACTCGCTCAGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATTAGGCGTTCACGCCGCATCTGGCATTCAGTACACAAAACCGATTAATTgccg                                                                                                                <  2:352963/146‑1 (MQ=255)
                                         cTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGa                                                                                                           <  2:1164262/150‑1 (MQ=255)
                                          tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCGTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                                          >  1:1312737/1‑150 (MQ=255)
                                          tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                                                                                                          >  2:1386576/1‑150 (MQ=255)
                                           gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg                                                                                                         >  2:1823991/1‑150 (MQ=255)
                                             aTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTc                                                                                                       <  2:1157703/150‑1 (MQ=255)
                                                 gTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgc                                                                                                   >  1:539009/1‑150 (MQ=255)
                                                   tGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcgc                                                                                                 >  1:744565/1‑150 (MQ=255)
                                                    gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAGTATGCCTGATTACGGTATTGCGAGTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCCTTCAGCACACAACACCGAGTAATTGCCGCCAGACGTCCGGCGCt                                                                                                >  1:313106/1‑150 (MQ=255)
                                                      aaaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc                                                                                              >  2:1960991/1‑150 (MQ=255)
                                                       aaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCCGGCCAGATAAGGCGTTCACGCCGCTTCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa                                                                                             >  1:1830979/1‑150 (MQ=255)
                                                          aaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGACGCCAGACGTCCGGCGCTGCAAtt                                                                                          >  2:612535/1‑150 (MQ=255)
                                                           aCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTg                                                                                         <  2:692298/150‑1 (MQ=255)
                                                            ccccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTgg                                                                                        >  1:215165/1‑150 (MQ=255)
                                                             cccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt                                                                                       <  2:403246/150‑1 (MQ=255)
                                                               cGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcg                                                                                     >  1:430434/1‑150 (MQ=255)
                                                                 cTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgcg                                                                                   <  2:751537/150‑1 (MQ=255)
                                                                 cTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgcg                                                                                   <  1:213631/150‑1 (MQ=255)
                                                                         gggTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcg                                                                           <  1:715750/150‑1 (MQ=255)
                                                                           gTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                                         >  2:904909/1‑150 (MQ=255)
                                                                           gTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggc                                                                         <  2:1524648/150‑1 (MQ=255)
                                                                             tttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggcgg                                                                       <  2:1699352/150‑1 (MQ=255)
                                                                             tttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTcggcgg                                                                       <  1:1855337/150‑1 (MQ=255)
                                                                              ttttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCggcggc                                                                      <  2:1713031/150‑1 (MQ=255)
                                                                                ttGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGgcggcgg                                                                    >  2:564225/1‑149 (MQ=255)
                                                                                  gTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGc                                                                  >  2:566593/1‑150 (MQ=255)
                                                                                   tATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGcc                                                                 <  1:130239/150‑1 (MQ=255)
                                                                                     tCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCTGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCtt                                                               >  2:1618155/1‑150 (MQ=255)
                                                                                      cTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCTGCCAGACGTCCGGCGCTGCAATTGGTCGCGAAAATTCGGCGGCGTGCCttt                                                              >  2:1503682/1‑150 (MQ=255)
                                                                                        gCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTaa                                                            <  1:209844/150‑1 (MQ=255)
                                                                                         cAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAAt                                                           <  1:398924/150‑1 (MQ=255)
                                                                                         cAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAAt                                                           <  2:1770934/150‑1 (MQ=255)
                                                                                          aGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATg                                                          <  1:1776759/150‑1 (MQ=255)
                                                                                           gATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATgg                                                         >  2:1212459/1‑150 (MQ=255)
                                                                                             ttATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTc                                                       <  1:55716/150‑1 (MQ=255)
                                                                                               aTGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCaa                                                     <  1:1024680/150‑1 (MQ=255)
                                                                                                tGCCTGATTACGGAATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAACCCGATAAATTGCCGCCAGACGGCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAg                                                    <  2:261247/150‑1 (MQ=255)
                                                                                                    tGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTAt                                                >  2:1792066/1‑150 (MQ=255)
                                                                                                     gATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTCGCATTCAGAACACAACACCGATTAATTGCCGCCAGACGACCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGACAAGGTATc                                               >  2:1342687/1‑150 (MQ=255)
                                                                                                     gATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGGTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTACCTTTAATGGTCAAGGTATc                                               <  1:731431/150‑1 (MQ=255)
                                                                                                          cGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTg                                          >  1:630388/1‑150 (MQ=255)
                                                                                                           ggTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCTGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGc                                         <  1:361446/150‑1 (MQ=255)
                                                                                                             tATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg                                       <  1:1548007/150‑1 (MQ=255)
                                                                                                             tATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg                                       <  1:1761010/150‑1 (MQ=255)
                                                                                                               ttGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGa                                     <  1:345946/150‑1 (MQ=255)
                                                                                                                 gCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATc                                   >  1:1735931/1‑150 (MQ=255)
                                                                                                                  cTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGCCGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATcc                                  >  1:1740306/1‑150 (MQ=255)
                                                                                                                  cTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATcc                                  >  1:930290/1‑150 (MQ=255)
                                                                                                                     ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                               <  1:336674/150‑1 (MQ=255)
                                                                                                                     ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                               >  1:192726/1‑150 (MQ=255)
                                                                                                                     ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                               <  2:1103415/150‑1 (MQ=255)
                                                                                                                     ttttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCGGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCag                               <  2:1298861/150‑1 (MQ=255)
                                                                                                                     tttcttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTAAGAACACACAACCGATTCATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATTGTCAAGGTATCGGTTGCTGGATCCCag                               <  1:108659/150‑1 (MQ=255)
                                                                                                                      tttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCaga                              >  2:899840/1‑150 (MQ=255)
                                                                                                                      tttttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCaga                              <  2:48711/150‑1 (MQ=255)
                                                                                                                        tttGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCagaag                            <  1:63725/150‑1 (MQ=255)
                                                                                                                         ttGCAGGCCAGATAAGGCGTTCACGCAGCATCTGGAATTCAGAACACAAACCCGATTAATTGCAGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGTATCCAagaaga                           >  1:1591152/1‑150 (MQ=255)
                                                                                                                          tGCAGGCCAGATAAGGCGTTAACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGCCGTCCGGCGATGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGAt                          <  2:186188/150‑1 (MQ=255)
                                                                                                                           gCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATg                         <  2:1666432/150‑1 (MQ=255)
                                                                                                                            cAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGc                        >  2:499632/1‑150 (MQ=255)
                                                                                                                            cAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGc                        >  2:74819/1‑150 (MQ=255)
                                                                                                                             atgCCAGATGCGGCGTGAACGCCTTATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGAGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCTGAAGATGCg                       >  1:247635/3‑150 (MQ=255)
                                                                                                                             aGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCg                       <  2:746029/150‑1 (MQ=255)
                                                                                                                              ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt                      >  1:1804455/1‑150 (MQ=255)
                                                                                                                              ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt                      >  2:847968/1‑150 (MQ=255)
                                                                                                                              ggCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGt                      >  1:421803/1‑150 (MQ=255)
                                                                                                                                 cAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGGTCCCAGAAGATGCGTTCa                   >  2:1073029/1‑150 (MQ=255)
                                                                                                                                   gATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTcccc                 >  1:117673/1‑150 (MQ=255)
                                                                                                                                   gATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAAGTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCAcc                 <  1:413062/150‑1 (MQ=255)
                                                                                                                                     tAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTa               >  2:1919726/1‑150 (MQ=255)
                                                                                                                                      gcggCGTGAACGCCTTATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGACGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTaa              >  2:1882350/3‑150 (MQ=255)
                                                                                                                                           gTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGGTGCTGGATCCCAGAAGAGGCGTTCACCTAACAgca         >  2:1331215/1‑150 (MQ=255)
                                                                                                                                             tCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTAGGCGGCGTGCCTTTAATGGTCAATGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatc       <  1:274532/150‑1 (MQ=255)
                                                                                                                                              cACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcg      >  1:1672439/1‑150 (MQ=255)
                                                                                                                                                cgccgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca    <  2:1562713/150‑1 (MQ=255)
                                                                                                                                                 gccgcATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgcatcgcat   >  2:457971/1‑150 (MQ=255)
                                                                                                                                                  ccgcATCTGGCATTCAGACCACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGAAAAGTCGGCGGCGTGCCGTTAAGGGGCAAGGTATCGGTGGCTGGATCCCAGAAGAGGCGTTCACCTAACAgcatcgcagc  <  1:1533141/150‑3 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATC  >  NC_000913/2282764‑2283059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: