Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,311 C→G 7.1% intergenic (+24/+79) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3110CG 7.1% 406.2 / 20.2 142intergenic (+24/+79)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (63/66);  new base G (3/7);  total (68/74)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

Only 100 of 146 total aligned reads displayed.

ATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTG  >  NC_000913/2286162‑2286459
                                                                                                                                                      |                                                                                                                                                     
atCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAAccc                                                                                                                                                       >  1:1729898/1‑150 (MQ=255)
 tCTAACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCt                                                                                                                                                      >  1:695509/1‑150 (MQ=255)
  cTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCtt                                                                                                                                                     >  1:1404772/1‑150 (MQ=255)
    tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                   <  1:1796272/150‑1 (MQ=255)
    tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTAATGGCGTGACGGGGGTCGGAGGTTCAAATACTCTCGGGACGACCAAAAATCACAAGAAAAAACCAAcccaaac                                                                                                                                                   >  2:776901/1‑146 (MQ=255)
          cAATCGGTGTGGAAATCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttgg                                                                                                                                             <  2:1867380/150‑1 (MQ=255)
          cAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttgg                                                                                                                                             >  1:141021/1‑150 (MQ=255)
           aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                            <  2:52917/150‑1 (MQ=255)
            aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggtt                                                                                                                                           <  1:1055598/150‑1 (MQ=255)
               ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCTAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                       >  2:1784666/1‑150 (MQ=255)
               ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                       >  2:1017339/1‑150 (MQ=255)
               ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                       >  2:579332/1‑150 (MQ=255)
               ggTGTGGAAAACGGTAGTATTAGCAGCCACGAGGCGGCACGTAGCGCAGCCTGGGAGCGCACCGTCATGGGGTGTCGGTGGGCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                       <  1:839247/150‑1 (MQ=255)
                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCATGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                      >  2:655525/1‑150 (MQ=255)
                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                      <  1:474874/150‑1 (MQ=255)
                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGGCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                      <  1:490497/150‑1 (MQ=255)
                 tgtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGGCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACTCTTACGGTTGGttttttt                                                                                                                                     <  2:882374/150‑1 (MQ=255)
                  gtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                                                                                    <  2:581210/150‑3 (MQ=255)
                  gtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                                                                                    <  2:1077228/150‑1 (MQ=255)
                   tgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                                                                                   >  1:760613/1‑150 (MQ=255)
                    ggAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttg                                                                                                                                  <  2:797131/150‑5 (MQ=255)
                    ggAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                                                                  >  1:896093/1‑150 (MQ=255)
                    ggAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACAAACCATTACGGTTGGTTTTTTtata                                                                                                                                  >  2:1873267/1‑150 (MQ=255)
                     gAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAAACCAACCGTAAGGGTTGGttttttcttg                                                                                                                                  <  1:1653645/150‑5 (MQ=255)
                         aCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGc                                                                                                                             <  1:1073678/150‑1 (MQ=255)
                          cGGTAGTAGTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCa                                                                                                                            <  1:558569/150‑1 (MQ=255)
                            gtagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                                                                          >  1:1454420/1‑150 (MQ=255)
                              agtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACAAACCCTTACGGTTGGTTTTTTTATATCTGCaatta                                                                                                                        <  2:1941405/150‑1 (MQ=255)
                                   tAGCAGCCACGAGTCGGCACGTAGCGGAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                   <  2:910384/150‑1 (MQ=255)
                                   tAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                   <  1:1702891/150‑1 (MQ=255)
                                   tAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                   <  2:1470900/150‑1 (MQ=255)
                                     gcagcCCCGCGTCGGCACGTATCGCAGCCTGGTAGCGCACCGTCATGGGGGGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGGTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                                 <  1:1270712/150‑1 (MQ=255)
                                     gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCGTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCGTACGGTTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                                 >  1:1966635/1‑150 (MQ=255)
                                     gcagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCAGGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGCCCAAAAATCCCAAGAAATAACCAACCCTTACGGTTGGTTGTTTTATATCTGCAATTAATTCGAt                                                                                                                 >  2:1721560/1‑150 (MQ=255)
                                      cagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGGCGGGGGGCGGAGGTTCAAATCCTCTCGTGCCGCCCAAAAATCCCAAGAAAAAACCTACCCTTACGGTGGGTTTTTTTATATCTGCAATTAATTCGATa                                                                                                                >  2:1350409/1‑150 (MQ=255)
                                        gcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                              >  2:400651/1‑150 (MQ=255)
                                        gcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                              <  1:1540675/150‑1 (MQ=255)
                                         ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                                                                             >  2:1281412/1‑150 (MQ=255)
                                          cACGAGTCGGCACGTAGCGCATCCTGGTATCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                                                                            <  1:1951246/150‑1 (MQ=255)
                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAg                                                                                                           >  2:191488/1‑150 (MQ=255)
                                                  ggCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTGACGGTTGGTTTTTTTATATCAGCAATTAATTCGATAAACAGACCgaga                                                                                                    >  2:357450/1‑150 (MQ=255)
                                                     aCGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACAGTGacac                                                                                                 >  2:495658/1‑150 (MQ=255)
                                                      cGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGCCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGGTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca                                                                                                >  1:1081108/1‑150 (MQ=255)
                                                        tAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTATTTTGATATCTGCAATTAATTCGATAAACAGACCGTGACACAt                                                                                               >  1:1389290/1‑150 (MQ=255)
                                                                 cTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGGTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGGCACATCACAGCCTGt                                                                                     >  2:1974359/1‑150 (MQ=255)
                                                                    gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTa                                                                                  <  1:1577138/150‑1 (MQ=255)
                                                                       gcgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAttt                                                                               >  2:1413745/1‑150 (MQ=255)
                                                                       gcgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCAGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTGTAGATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAttt                                                                               >  1:334314/1‑150 (MQ=255)
                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                                                                           <  2:1473125/150‑1 (MQ=255)
                                                                            ccGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGt                                                                          <  2:497787/150‑1 (MQ=255)
                                                                                cATGGGGTGTCGGTGGTCTGAGGTTCAAATCCTCTCGTGACGACCAAAAATCCCAAGAAAAATCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCAATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                      <  1:1041963/150‑1 (MQ=255)
                                                                                cATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                      <  2:653582/150‑1 (MQ=255)
                                                                                cATGGGGGGTCGGGGGTCTGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGTTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                                                                      <  2:1223460/150‑1 (MQ=255)
                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                                                                     <  1:413561/150‑1 (MQ=255)
                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                                                                     >  2:636924/1‑150 (MQ=255)
                                                                                   ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACAACCCTTACGGTTGGTTTTTTTTTTTATGGAATTTATTACAATAACAGCCCGTGACCCCTCCCAGCCGTGTTATTTTCTGTTTTGAGaa                                                                  >  2:1513205/1‑150 (MQ=255)
                                                                                    gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGaa                                                                  >  1:741137/1‑150 (MQ=255)
                                                                                      gtgtCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg                                                                >  1:1347095/1‑150 (MQ=255)
                                                                                          cGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCa                                                            <  2:994068/150‑1 (MQ=255)
                                                                                           gggggTCGGAGGGTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTAGATAAACAGACCGTGACACCTCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAg                                                           <  2:2004463/150‑1 (MQ=255)
                                                                                              ggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAcc                                                        <  1:612020/150‑1 (MQ=255)
                                                                                               gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACca                                                       <  2:98146/150‑1 (MQ=255)
                                                                                                tCGGAGGTTCAAATCCTCTCGTGCAGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGCCACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACcac                                                      <  1:125663/150‑1 (MQ=255)
                                                                                                  ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac                                                    >  2:2015952/1‑150 (MQ=255)
                                                                                                     ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCg                                                 >  1:421419/1‑150 (MQ=255)
                                                                                                       ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCAAGACCACACCCGcc                                               >  1:621084/1‑150 (MQ=255)
                                                                                                        tCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCACCCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGCCCGTGACACATCACAGCGTGTTTATTTTCTGTTATCAGAACGTCCAGCCCCCACCCGccg                                              >  1:1091751/1‑149 (MQ=255)
                                                                                                        tCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                              >  1:1809130/1‑150 (MQ=255)
                                                                                                          aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCCCACCCGCCTGa                                            >  1:333674/1‑150 (MQ=255)
                                                                                                           aaTCCTCTCGTGCCTACCAAAAATCCGAAGAAAAAACCAAACATTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGTCCACACCCGCCTGAg                                           <  2:1328964/150‑1 (MQ=255)
                                                                                                           aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                           <  1:1440374/150‑1 (MQ=255)
                                                                                                           aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                           <  2:896093/150‑1 (MQ=255)
                                                                                                           aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTAGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAg                                           >  2:871711/1‑150 (MQ=255)
                                                                                                               ctctCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTGCGGTTGGTTTTTTTAGATCTGCATTTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGATCGTCCAGACCACACCCGCCTGAGTAtt                                       >  2:1435252/1‑150 (MQ=255)
                                                                                                               ctctCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCATTACGGTTGGTTTTTTTATATCTGCAATTAAATCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                       >  1:1607385/1‑150 (MQ=255)
                                                                                                                     tGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACgg                                 <  1:1041544/150‑1 (MQ=255)
                                                                                                                           ccAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGCCACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACGCCACCCGCCTGAGTATTCCACGGCGATTc                           >  1:68121/1‑150 (MQ=255)
                                                                                                                            cAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCt                          >  2:236268/1‑150 (MQ=255)
                                                                                                                            cAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCt                          >  2:1563883/1‑150 (MQ=255)
                                                                                                                             aaaaaTCCCAAGAAAAAACCACCCCTTACGGTTGGTTTTTTTATTTCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATAAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTa                         >  2:162910/1‑150 (MQ=255)
                                                                                                                             aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCACCGGCGATTCTa                         >  1:1020993/1‑150 (MQ=255)
                                                                                                                             aaaaaTCCCAAGAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                        >  2:392673/1‑150 (MQ=255)
                                                                                                                              aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                        <  2:1063518/150‑1 (MQ=255)
                                                                                                                              aaaaTACCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGACTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                        <  2:237086/150‑1 (MQ=255)
                                                                                                                               aaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAcc                       <  2:1139811/150‑1 (MQ=255)
                                                                                                                                    ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                  >  1:1514290/1‑150 (MQ=255)
                                                                                                                                    ccAAGAAAAAACCAACCCTTACGGTTGGGTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTTTTTTCTGTTATCAGAACGTCAAGACCACACCCGCCTGAGTATTCGACGGCGATTCTACCCCTgc                  >  2:1247746/1‑150 (MQ=255)
                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcg                 <  2:931065/150‑1 (MQ=255)
                                                                                                                                      aaGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcgc                <  1:579332/150‑1 (MQ=255)
                                                                                                                                        gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa              <  2:1929054/150‑1 (MQ=255)
                                                                                                                                         aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa              >  2:1677112/2‑150 (MQ=255)
                                                                                                                                          aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTATGTTCTCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGAGTCTACCCCTGCGCCAt             >  2:228529/1‑150 (MQ=255)
                                                                                                                                           aaaatCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt            <  1:684923/150‑1 (MQ=255)
                                                                                                                                           aaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt            >  1:880721/1‑150 (MQ=255)
                                                                                                                                             aaaccaaaccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGATAGTCCATACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGc          <  2:1691461/150‑1 (MQ=255)
                                                                                                                                             aaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGc          <  1:1871210/150‑1 (MQ=255)
                                                                                                                                             aaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGc          <  2:554445/150‑1 (MQ=255)
                                                                                                                                                ccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt       >  2:932004/1‑150 (MQ=255)
                                                                                                                                                  aaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTACTGTTATCAGAACGTCCAGACCACACCCGCCGAAGAATTCCACGGCAATTCAACCCCTCCCCAATTGCCaaaa     >  1:779424/1‑147 (MQ=255)
                                                                                                                                                     ccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGGCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctg  <  2:334314/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTG  >  NC_000913/2286162‑2286459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: