Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,290,448 A→G 5.3% intergenic (‑268/‑52) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,290,4480AG 5.3% 458.5 / 11.2 151intergenic (‑268/‑52)yejO/narPpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
Reads supporting (aligned to +/- strand):  ref base A (73/69);  new base G (3/5);  total (77/74)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 153 total aligned reads displayed.

ATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCT  >  NC_000913/2290299‑2290593
                                                                                                                                                     |                                                                                                                                                 
attgattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTa                                                                                                                                                   <  1:701754/150‑1 (MQ=255)
attgattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTa                                                                                                                                                   >  1:850857/1‑150 (MQ=255)
 ttgattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTAc                                                                                                                                                  >  2:865707/1‑150 (MQ=255)
      tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacg                                                                                                                                             <  1:919795/150‑1 (MQ=255)
       ttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgc                                                                                                                                            <  2:14354/150‑1 (MQ=255)
       ttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgc                                                                                                                                            <  1:231300/150‑1 (MQ=255)
           aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacg                                                                                                                                        <  2:726076/150‑1 (MQ=255)
           aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCCCCTGGTAGCGTTGGTAacgctacc                                                                                                                                        <  2:615432/150‑2 (MQ=255)
            gttgatTTTCTTGATGAGAAGCTGATGCAAAAATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTATCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacca                                                                                                                                       <  1:1573196/150‑3 (MQ=255)
             ttgttTTTCTTGATGAGAATCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccag                                                                                                                                      <  2:1357214/150‑4 (MQ=255)
              tgttTTTCTTGATGAGAAGCTGATGCAAAATTCGGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTACCGTTACCAACGCTACGCTc                                                                                                                                     >  2:1637424/1‑150 (MQ=255)
              tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccagg                                                                                                                                     <  1:1759071/150‑5 (MQ=255)
              tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTc                                                                                                                                     >  1:935277/1‑150 (MQ=255)
               gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCa                                                                                                                                    <  1:553452/150‑1 (MQ=255)
               gttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCAAAAGCGAACGACAGGGTTGTAGTTTTCACTACATGTCCATACATAAAAAGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCa                                                                                                                                    >  1:422678/1‑150 (MQ=255)
                tttttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaa                                                                                                                                   >  1:1726789/1‑150 (MQ=255)
                tttttCTTGATGAGAAGCTGATGCAAAATACCGTCTTTAAAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccaggcg                                                                                                                                   <  2:771395/150‑7 (MQ=255)
                  tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACCCTACGCTCAAAc                                                                                                                                 >  1:873431/1‑150 (MQ=255)
                  ttacTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                 <  1:1018613/147‑1 (MQ=255)
                     cTTGATGAGAAGCTGATTCAAAATTCCGTCTTTATAATGAAATTGATGCCAAAGCGAACGACAAGGTGGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATa                                                                                                                              <  1:295578/150‑1 (MQ=255)
                      ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                             >  1:1909629/1‑150 (MQ=255)
                      ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                             >  1:1134011/1‑150 (MQ=255)
                         atgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGa                                                                                                                          >  2:1814385/1‑150 (MQ=255)
                          tgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGAt                                                                                                                         >  1:340269/1‑150 (MQ=255)
                              aaGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCta                                                                                                                     <  2:484331/150‑1 (MQ=255)
                                  tGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGACTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaataa                                                                                                                 >  1:1372202/1‑150 (MQ=255)
                                    aTGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATaaaa                                                                                                               >  1:239282/1‑150 (MQ=255)
                                     tGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAAc                                                                                                              <  1:824958/150‑1 (MQ=255)
                                     tGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAAc                                                                                                              >  1:802594/1‑150 (MQ=255)
                                       cAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCt                                                                                                            >  1:2016334/1‑150 (MQ=255)
                                       cAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCt                                                                                                            >  2:702610/1‑150 (MQ=255)
                                           aTTCCGTCTTTATAATGGAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAgg                                                                                                        <  2:302720/150‑1 (MQ=255)
                                             tCCGTCTTTATAATGAAAATTATGCAAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACTCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGgag                                                                                                      <  1:101952/150‑1 (MQ=255)
                                             tCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGgag                                                                                                      <  1:1758948/150‑1 (MQ=255)
                                               cGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGTTAACATTCACGCTCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAAAAAAACCTCAGGAGac                                                                                                    >  1:949096/1‑150 (MQ=255)
                                                gTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGact                                                                                                   <  2:63275/150‑1 (MQ=255)
                                                  cTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactac                                                                                                 <  1:1129376/150‑1 (MQ=255)
                                                   aatataATGAAAATGATGCCAACGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTAACCAACGCAACTCACAAACATAAACATTCTAATAAAACCTAAGGAGactact                                                                                                >  2:1875309/3‑150 (MQ=255)
                                                      ataATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCCCTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATg                                                                                             >  1:1842201/1‑150 (MQ=255)
                                                        aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                           >  2:1921042/1‑150 (MQ=255)
                                                         aTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCt                                                                                          <  2:266420/150‑1 (MQ=255)
                                                          tGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGCGGTAACATACACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGAATCTAATAAAACCTCAGGAGACTACAAAGCCTg                                                                                         >  1:273728/1‑150 (MQ=255)
                                                           gAAAATGATGCCAAAGCGAACGACAAGGTTTTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTATCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACATCAGGATACAACTATGCCTGa                                                                                        >  1:380724/1‑150 (MQ=255)
                                                           gAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGa                                                                                        >  2:1661012/1‑150 (MQ=255)
                                                           gAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGa                                                                                        >  2:22509/1‑150 (MQ=255)
                                                            aaaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGaa                                                                                       <  2:629410/150‑1 (MQ=255)
                                                             aaaTGATGCCAAAGCTAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                      <  1:143054/150‑1 (MQ=255)
                                                             aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                      <  2:1427484/150‑1 (MQ=255)
                                                             aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                      <  2:1151161/150‑1 (MQ=255)
                                                              aaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGc                                                                                     <  2:404734/150‑1 (MQ=255)
                                                              aaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTAAAATGCCTGAAGc                                                                                     >  1:1350757/1‑150 (MQ=255)
                                                                tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAACCCTCAGGAGACTACTATGCCTGAAGCaa                                                                                   >  2:1133576/1‑150 (MQ=255)
                                                                tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTAAGATCAAACATAATTATGCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCac                                                                                   >  2:114624/1‑149 (MQ=255)
                                                                 gatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAac                                                                                  <  2:1081911/150‑1 (MQ=255)
                                                                  atgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAaca                                                                                 <  1:1404043/150‑1 (MQ=255)
                                                                   tgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAacac                                                                                >  1:381849/1‑150 (MQ=255)
                                                                     ccAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCt                                                                              >  2:492177/1‑150 (MQ=255)
                                                                     ccAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCt                                                                              >  1:1708718/1‑150 (MQ=255)
                                                                      cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                             <  2:341557/150‑1 (MQ=255)
                                                                           cGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAg                                                                        >  2:424754/1‑150 (MQ=255)
                                                                              aCGTCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtg                                                                     <  1:1646493/150‑1 (MQ=255)
                                                                               cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                    >  1:1690353/1‑150 (MQ=255)
                                                                                gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                   <  2:1503155/150‑1 (MQ=255)
                                                                                 aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGCAGCAACACCTTTTCAGGtgatg                                                                  >  2:1295129/1‑150 (MQ=255)
                                                                                  cAAGGTTGTAGTTTTCACTACATGTCAATACATAAAATGGGGTAAAATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCATGAAGCAACAACTTTTCAGGtgatga                                                                 >  2:1133602/1‑150 (MQ=255)
                                                                                   aaGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCGTTTCAGGTgatgat                                                                >  1:524318/1‑150 (MQ=255)
                                                                                      gTTGTAGTTTTCACTACATGTCCATACATAATATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgt                                                             <  2:792230/150‑1 (MQ=255)
                                                                                      gTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAACCCTAAGGAGACTACTATGACTGAAGCAACACCTTTTCAGGTGATGATtgt                                                             <  2:1599363/150‑1 (MQ=255)
                                                                                           aGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatg                                                        >  2:1919868/1‑150 (MQ=255)
                                                                                            gTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCACCACCTTTTCAGGGGATGATTGTGgatga                                                       >  2:385862/1‑150 (MQ=255)
                                                                                            gTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatga                                                       <  2:802594/150‑1 (MQ=255)
                                                                                             ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                      <  1:264566/150‑1 (MQ=255)
                                                                                             ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                      <  1:2011549/150‑1 (MQ=255)
                                                                                             ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                      <  1:650354/150‑1 (MQ=255)
                                                                                             ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                      <  2:1068129/150‑1 (MQ=255)
                                                                                              tttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGatc                                                     >  1:808263/1‑150 (MQ=255)
                                                                                                tCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTAAGGAGACTACTATGCCTGAAGCAACACATTTTCAGGTCATGATTGTGGATGatcat                                                   >  2:1239383/1‑150 (MQ=255)
                                                                                                    tACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAGCACCTTTTCAGGTGATGATTGTGGATGATCATCCAc                                               <  1:350169/150‑1 (MQ=255)
                                                                                                     aCATGTCCATACAAAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACt                                              <  2:165308/150‑1 (MQ=255)
                                                                                                       aTGTCCATACATAAAATGGGTTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCAcaat                                            <  2:1780900/150‑4 (MQ=255)
                                                                                                        tGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTAt                                           <  2:1581849/150‑1 (MQ=255)
                                                                                                         gTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATg                                          >  1:1569262/1‑150 (MQ=255)
                                                                                                          tCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGc                                         >  1:993917/1‑150 (MQ=255)
                                                                                                               acataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgc                                    <  1:1442987/150‑1 (MQ=255)
                                                                                                                 ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCgg                                  <  1:325766/150‑1 (MQ=255)
                                                                                                                    aaaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgt                               <  2:822716/150‑1 (MQ=255)
                                                                                                                       gggggGAACATTCACGCGCCTGTTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTATCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCg                            <  2:328553/149‑1 (MQ=255)
                                                                                                                           gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAg                        <  1:1351967/150‑1 (MQ=255)
                                                                                                                               cATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTaa                    >  2:2010458/1‑149 (MQ=255)
                                                                                                                                   cACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGa                >  1:278745/1‑150 (MQ=255)
                                                                                                                                       cgcCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGTTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCtt            >  1:335582/1‑150 (MQ=255)
                                                                                                                                        tgagcgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCCTg           >  2:612474/6‑150 (MQ=255)
                                                                                                                                          cTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGAt         >  1:1626341/1‑150 (MQ=255)
                                                                                                                                           gcgTAGCGTTGGTAATGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc        >  2:1550816/3‑150 (MQ=255)
                                                                                                                                            ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc       >  2:1346651/1‑150 (MQ=255)
                                                                                                                                            ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc       <  2:1532359/150‑1 (MQ=255)
                                                                                                                                            cgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc       >  1:604303/2‑150 (MQ=255)
                                                                                                                                                gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc   >  1:256693/1‑150 (MQ=255)
                                                                                                                                                gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc   <  2:1562784/150‑1 (MQ=255)
                                                                                                                                                 cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGGTCGTCAGTTACTGGAGCTTGATCCTGGct  >  2:1784142/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCT  >  NC_000913/2290299‑2290593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: