Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,290,449 C→G 5.4% intergenic (‑269/‑51) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,290,4490CG 5.4% 468.7 / 13.9 148intergenic (‑269/‑51)yejO/narPpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
Reads supporting (aligned to +/- strand):  ref base C (72/68);  new base G (3/5);  total (75/73)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 152 total aligned reads displayed.

TTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAA  >  NC_000913/2290300‑2290598
                                                                                                                                                     |                                                                                                                                                     
ttgattTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTAc                                                                                                                                                       >  2:865707/1‑150 (MQ=255)
     tttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacg                                                                                                                                                  <  1:919795/150‑1 (MQ=255)
      ttttAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgc                                                                                                                                                 <  2:14354/150‑1 (MQ=255)
          aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAacgctacg                                                                                                                                             <  2:726076/150‑1 (MQ=255)
          aGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCCCCTGGTAGCGTTGGTAacgctacc                                                                                                                                             <  2:615432/150‑2 (MQ=255)
           gttgatTTTCTTGATGAGAAGCTGATGCAAAAATCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTATCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctacca                                                                                                                                            <  1:1573196/150‑3 (MQ=255)
            ttgttTTTCTTGATGAGAATCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccag                                                                                                                                           <  2:1357214/150‑4 (MQ=255)
             tgttTTTCTTGATGAGAAGCTGATGCAAAATTCGGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTACCGTTACCAACGCTACGCTc                                                                                                                                          >  2:1637424/1‑150 (MQ=255)
             tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTGGTAacgctaccagg                                                                                                                                          <  1:1759071/150‑5 (MQ=255)
             tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTc                                                                                                                                          >  1:935277/1‑150 (MQ=255)
             tgttTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTc                                                                                                                                          >  2:1592117/1‑150 (MQ=255)
               tttttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCaa                                                                                                                                        >  1:1726789/1‑150 (MQ=255)
                 tttCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACCCTACGCTCAAAc                                                                                                                                      >  1:873431/1‑150 (MQ=255)
                 ttacTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAAc                                                                                                                                      <  1:1018613/147‑1 (MQ=255)
                    cTTGATGAGAAGCTGATTCAAAATTCCGTCTTTATAATGAAATTGATGCCAAAGCGAACGACAAGGTGGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATa                                                                                                                                   <  1:295578/150‑1 (MQ=255)
                     ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCATACATaa                                                                                                                                  >  1:394659/1‑150 (MQ=255)
                     ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                  >  1:1134011/1‑150 (MQ=255)
                     ttGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATaa                                                                                                                                  >  1:1909629/1‑150 (MQ=255)
                         tgaGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGAt                                                                                                                              >  1:340269/1‑150 (MQ=255)
                            gaAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCt                                                                                                                           <  2:1810252/150‑1 (MQ=255)
                             aaGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCta                                                                                                                          <  2:163840/150‑1 (MQ=255)
                               gCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaat                                                                                                                        >  1:538845/1‑150 (MQ=255)
                                 tGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGACTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCtaataa                                                                                                                      >  1:1372202/1‑150 (MQ=255)
                                   aTGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATaaaa                                                                                                                    >  1:210091/1‑150 (MQ=255)
                                   aTGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATaaaa                                                                                                                    >  1:239282/1‑150 (MQ=255)
                                    tGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAAc                                                                                                                   <  1:824958/150‑1 (MQ=255)
                                    tGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAAc                                                                                                                   >  1:802594/1‑150 (MQ=255)
                                    tGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAAc                                                                                                                   <  1:366555/150‑1 (MQ=255)
                                      cAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCt                                                                                                                 >  1:2016334/1‑150 (MQ=255)
                                      cAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCt                                                                                                                 >  2:623190/1‑150 (MQ=255)
                                      cAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACAAACGCTACGCTCAAACATAATGATTCTAATAAAAccg                                                                                                                 >  2:695469/1‑149 (MQ=255)
                                          aTTCCGTCTTTATAATGGAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAgg                                                                                                             <  2:302720/150‑1 (MQ=255)
                                            tCCGTCTTTATAATGAAAATTATGCAAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACTCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGgag                                                                                                           <  1:101952/150‑1 (MQ=255)
                                              cGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGTTAACATTCACGCTCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAAAAAAACCTCAGGAGac                                                                                                         >  1:949096/1‑150 (MQ=255)
                                               gTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGact                                                                                                        <  2:63275/150‑1 (MQ=255)
                                                 ttttATAATGAAAATGATGCCAAAGCGAACGACAAGTTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactac                                                                                                      <  1:1761228/149‑1 (MQ=255)
                                                 cTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGactac                                                                                                      <  1:1129376/150‑1 (MQ=255)
                                                  aatataATGAAAATGATGCCAACGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTAACCAACGCAACTCACAAACATAAACATTCTAATAAAACCTAAGGAGactact                                                                                                     >  2:1875309/3‑150 (MQ=255)
                                                       aaTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGcc                                                                                                >  2:1921042/1‑150 (MQ=255)
                                                        aTGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCt                                                                                               <  2:266420/150‑1 (MQ=255)
                                                         tGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGCGGTAACATACACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGAATCTAATAAAACCTCAGGAGACTACAAAGCCTg                                                                                              >  1:273728/1‑150 (MQ=255)
                                                          gAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGa                                                                                             >  2:1661012/1‑150 (MQ=255)
                                                          gAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGa                                                                                             >  2:22509/1‑150 (MQ=255)
                                                            aaaTGATGCCAAAGCTAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                           <  1:143054/150‑1 (MQ=255)
                                                            aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                           <  2:1151161/150‑1 (MQ=255)
                                                            aaaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAg                                                                                           <  2:1427484/150‑1 (MQ=255)
                                                             aaTGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTAAAATGCCTGAAGc                                                                                          >  1:1350757/1‑150 (MQ=255)
                                                               tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAACCCTCAGGAGACTACTATGCCTGAAGCaa                                                                                        >  2:1133576/1‑150 (MQ=255)
                                                               tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCaa                                                                                        <  2:749489/150‑1 (MQ=255)
                                                               tgatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTAAGATCAAACATAATTATGCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCac                                                                                        >  2:114624/1‑149 (MQ=255)
                                                                gatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAac                                                                                       <  2:1749771/150‑1 (MQ=255)
                                                                gatgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAac                                                                                       <  2:1081911/150‑1 (MQ=255)
                                                                 atgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAaca                                                                                      <  1:1404043/150‑1 (MQ=255)
                                                                  tgCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAacac                                                                                     >  1:381849/1‑150 (MQ=255)
                                                                    ccAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCt                                                                                   <  1:817327/150‑1 (MQ=255)
                                                                    ccAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCt                                                                                   >  1:1708718/1‑150 (MQ=255)
                                                                    ccAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCt                                                                                   >  2:492177/1‑150 (MQ=255)
                                                                     cAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCtt                                                                                  <  2:341557/150‑1 (MQ=255)
                                                                      aaaGCGAACGACAAGGTTGTAGTTTTCACTACATCTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCATTACCAACGCTACGATCAATCATAATGATTCTAATAAAACCACAGGAGACTACTAAGCATGAAGCAacacaaat                                                                                 >  1:617936/1‑146 (MQ=255)
                                                                          cGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAg                                                                             >  2:424754/1‑150 (MQ=255)
                                                                             aCGTCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtg                                                                          <  1:1646493/150‑1 (MQ=255)
                                                                              cGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtga                                                                         >  1:1690353/1‑150 (MQ=255)
                                                                               gACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGtgat                                                                        <  2:1503155/150‑1 (MQ=255)
                                                                                aCAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGCAGCAACACCTTTTCAGGtgatg                                                                       >  2:1295129/1‑150 (MQ=255)
                                                                                 cAAGGTTGTAGTTTTCACTACATGTCAATACATAAAATGGGGTAAAATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCATGAAGCAACAACTTTTCAGGtgatga                                                                      >  2:1133602/1‑150 (MQ=255)
                                                                                  aaGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCGTTTCAGGTgatgat                                                                     >  1:524318/1‑150 (MQ=255)
                                                                                     gTTGTAGTTTTCACTACATGTCCATACATAATATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATtgt                                                                  <  2:792230/150‑1 (MQ=255)
                                                                                          aGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatg                                                             >  2:1919868/1‑150 (MQ=255)
                                                                                           gTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCACCACCTTTTCAGGGGATGATTGTGgatga                                                            >  2:385862/1‑150 (MQ=255)
                                                                                           gTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatga                                                            <  2:802594/150‑1 (MQ=255)
                                                                                            ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                           <  1:264566/150‑1 (MQ=255)
                                                                                            ttttCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGgatgat                                                           <  2:1068129/150‑1 (MQ=255)
                                                                                               tCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTAAGGAGACTACTATGCCTGAAGCAACACATTTTCAGGTCATGATTGTGGATGatcat                                                        >  2:1239383/1‑150 (MQ=255)
                                                                                                   tACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAGCACCTTTTCAGGTGATGATTGTGGATGATCATCCAc                                                    <  1:350169/150‑1 (MQ=255)
                                                                                                       tGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTAt                                                <  2:1581849/150‑1 (MQ=255)
                                                                                                        gTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATg                                               >  1:1569262/1‑150 (MQ=255)
                                                                                                         tCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGc                                              >  1:993917/1‑150 (MQ=255)
                                                                                                             tacataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcg                                          >  1:1929425/1‑150 (MQ=255)
                                                                                                              acataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGAcgc                                         <  1:1442987/150‑1 (MQ=255)
                                                                                                              acataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGATACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTAAGGTGATGATTGTGGAAGATCATCCACTTATTCGAcgc                                         >  2:427330/1‑150 (MQ=255)
                                                                                                                ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCgg                                       <  1:730001/150‑1 (MQ=255)
                                                                                                                ataAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCgg                                       <  1:325766/150‑1 (MQ=255)
                                                                                                                   aaaTGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGgtgt                                    <  2:822716/150‑1 (MQ=255)
                                                                                                                      gggggGAACATTCACGCGCCTGTTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTATCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCg                                 <  2:328553/149‑1 (MQ=255)
                                                                                                                        gggTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTc                               <  1:1746873/150‑1 (MQ=255)
                                                                                                                         ggTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAg                             <  2:391676/150‑1 (MQ=255)
                                                                                                                          gTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAg                             <  1:1351967/150‑1 (MQ=255)
                                                                                                                              cATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTaa                         >  2:2010458/1‑149 (MQ=255)
                                                                                                                                  cACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGa                     >  1:278745/1‑150 (MQ=255)
                                                                                                                                      cgcCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGTTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCtt                 >  1:335582/1‑150 (MQ=255)
                                                                                                                                         cTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGAt              >  1:1626341/1‑150 (MQ=255)
                                                                                                                                          gcgTAGCGTTGGTAATGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATc             >  2:1550816/3‑150 (MQ=255)
                                                                                                                                           ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc            >  2:1346651/1‑150 (MQ=255)
                                                                                                                                           ggTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc            <  2:1532359/150‑1 (MQ=255)
                                                                                                                                           cgTAGCGTTGGTAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATcc            >  1:604303/2‑150 (MQ=255)
                                                                                                                                             tAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTg          <  2:523136/150‑1 (MQ=255)
                                                                                                                                               gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc        >  1:256693/1‑150 (MQ=255)
                                                                                                                                               gCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGc        <  2:1562784/150‑1 (MQ=255)
                                                                                                                                                cGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGGTCGTCAGTTACTGGAGCTTGATCCTGGct       >  2:1784142/1‑150 (MQ=255)
                                                                                                                                                     ccAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGaa  >  2:777604/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGATTTTTTAGTTGTTTTTCTTGATGAGAAGCTGATGCAAAATTCCGTCTTTATAATGAAAATGATGCCAAAGCGAACGACAAGGTTGTAGTTTTCACTACATGTCCATACATAAAATGGGGTAACATTCACGCGCCTGGTAGCGTTACCAACGCTACGCTCAAACATAATGATTCTAATAAAACCTCAGGAGACTACTATGCCTGAAGCAACACCTTTTCAGGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAA  >  NC_000913/2290300‑2290598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: