Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,413 A→T 5.6% intergenic (‑61/+26) yfbS ← / ← yfbT putative transporter/sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,4130AT 5.6% 352.9 / 5.2 125intergenic (‑61/+26)yfbS/yfbTputative transporter/sugar phosphatas
Reads supporting (aligned to +/- strand):  ref base A (61/54);  new base T (3/5);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGA  >  NC_000913/2411268‑2411562
                                                                                                                                                 |                                                                                                                                                     
ccGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                                                                                                                                                   >  2:765675/1‑150 (MQ=255)
   cATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCTCGTCGGGGCt                                                                                                                                                <  1:844435/150‑1 (MQ=255)
   cATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCt                                                                                                                                                >  2:1585401/1‑150 (MQ=255)
     tCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAGCGTTCACAACTTATCCTTGTTAATTGAGGGGGAGGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaa                                                                                                                                              >  2:1858130/1‑150 (MQ=255)
      ccATACGCACTCTGACCGTCTCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGCCTCGTCGGGGCtttt                                                                                                                                             <  2:1639917/150‑4 (MQ=255)
       cATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAt                                                                                                                                            <  2:837052/150‑1 (MQ=255)
          aCGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAt                                                                                                                                         >  2:893038/1‑150 (MQ=255)
           cGCACCCGGCCCGCCCCAAACAACCCGATGCCAACGGCCAGTAATGAACGACCCCAAATCAATTCACCGTCCCCAACTAAACCTTGTAAATTGAGGGGTATGCCTTGATTCGCCCAAAAAAACCCCCCGCCGAGACGGGGCTAAATCATg                                                                                                                                        <  2:1960770/150‑1 (MQ=255)
            gCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa                                                                                                                                       <  2:1610591/150‑1 (MQ=255)
             cacaCTGCCCGTCGAAAACAAGACGATGTCAACGCCCAGTAATGAACGAACCCAAATCAAATCACCGTTCACAACTTATCCTTGTTAATTGAGGGTGATCACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGTGGCTAAATCATGAt                                                                                                                                      <  1:1216365/150‑1 (MQ=255)
             cACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                                                                                                                                      <  2:689511/150‑1 (MQ=255)
              aCTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc                                                                                                                                     <  1:1505466/150‑1 (MQ=255)
               ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGCCTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa                                                                                                                                    >  2:83162/1‑150 (MQ=255)
               ctctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa                                                                                                                                    >  1:729078/1‑150 (MQ=255)
               atctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa                                                                                                                                    <  2:1843693/149‑1 (MQ=255)
                 ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                                                                                                                                  <  1:1068186/150‑1 (MQ=255)
                   gCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtg                                                                                                                                <  1:1513572/150‑1 (MQ=255)
                       gTCGCAAACAAGACGATGTGAACGGCCAGTAATGATATAACCCAAATCAATTCACCGTTCACATCTAATCCTTGTTATTTGAGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGTCGGGGCTAAATCATGTTCAGGTGTTTc                                                                                                                            <  1:87991/150‑1 (MQ=255)
                       gTCGCAAACAAGACGATGGCCACGGCCAGTAATTAAAGACCCCAAATCAATTCACCTTTCACAACTTATCCTTGTTAATTGTGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGCCGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                            <  1:538820/150‑1 (MQ=255)
                       gTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                            <  2:691398/150‑1 (MQ=255)
                         cGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCAc                                                                                                                          >  2:1817055/1‑150 (MQ=255)
                           cAAACAAGACGATGGCAACGGCCAGTAATGAAAGATCCCTAATCAATTCTCCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                        <  2:1047218/150‑1 (MQ=255)
                           cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                        <  2:297272/150‑1 (MQ=255)
                           cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                        >  2:571162/1‑150 (MQ=255)
                           aaaaCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGCTGACTTGATGCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                        <  1:1693602/149‑1 (MQ=255)
                             aaCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTTAATCATGATCAGGTGTTTCACTGaa                                                                                                                      >  2:169376/1‑150 (MQ=255)
                               cAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaata                                                                                                                    >  1:198871/1‑150 (MQ=255)
                                aaGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaataa                                                                                                                   <  1:1402430/150‑1 (MQ=255)
                                 aGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCTCTGaataat                                                                                                                  >  2:655915/1‑150 (MQ=255)
                                  gACCATGGCAACGGGCTGTTATGAAAGAACCCATATCATTTCACCGTTCACTACTTATCGTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCGAGACGGGGCTAATTGATGATCAGGTGTTTCACTGaataatt                                                                                                                 <  1:225131/150‑2 (MQ=255)
                                     gATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACa                                                                                                              <  1:1738367/150‑1 (MQ=255)
                                         gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGc                                                                                                          >  1:1047313/1‑150 (MQ=255)
                                         gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGc                                                                                                          >  2:709049/1‑150 (MQ=255)
                                          cAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGcc                                                                                                         >  2:129253/1‑150 (MQ=255)
                                          cAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAAAAATAACATCGcc                                                                                                         >  2:15476/1‑150 (MQ=255)
                                              ggCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCAttt                                                                                                     >  1:1235292/1‑150 (MQ=255)
                                                   gTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTg                                                                                                <  1:1906326/150‑1 (MQ=255)
                                                         aaaGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGt                                                                                          <  2:2015065/150‑1 (MQ=255)
                                                         aaaGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGt                                                                                          <  2:1084348/150‑1 (MQ=255)
                                                         aaaGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGt                                                                                          <  2:1934441/150‑1 (MQ=255)
                                                           aGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTca                                                                                        >  1:686798/1‑150 (MQ=255)
                                                               cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                                    >  2:349567/1‑150 (MQ=255)
                                                                 cAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTaa                                                                                  >  2:481973/1‑150 (MQ=255)
                                                                       aaTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCt                                                                            >  1:1724570/1‑150 (MQ=255)
                                                                         ttCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTcc                                                                          <  2:7106/150‑1 (MQ=255)
                                                                          tCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCAATAAAAAAGCCCCGACGAGACGGTGCTAAATCATGATCAGGTTTTTCACTGAAAAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCa                                                                         <  1:912410/150‑1 (MQ=255)
                                                                              cGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACt                                                                     >  1:1840969/1‑150 (MQ=255)
                                                                                ttCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACtgt                                                                   <  2:580363/150‑1 (MQ=255)
                                                                                  cacaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                                 >  2:1285735/1‑150 (MQ=255)
                                                                                  cacaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCGCCAGACTGTgg                                                                 >  2:1198282/1‑150 (MQ=255)
                                                                                  cacaACTTATCCTTGTAAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAATTCATGATCAGCTGTATCACTGAATAATAACATCGGCATTTGGCTGTTTGGTCACACTAATTTGCTCCAGACTGTgg                                                                 >  2:462481/1‑150 (MQ=255)
                                                                                  cacaACTTATCATTGTTAATTGAGGGGAATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGTGGCAAAAACATGATCAGGTGTTTCAATGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                                 >  2:938321/1‑150 (MQ=255)
                                                                                   acaACTTATCCTTGTTAATTGAGGGGGATGAATTGATTCTTCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                                <  2:67244/150‑1 (MQ=255)
                                                                                      aCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagga                                                             <  2:1656221/150‑1 (MQ=255)
                                                                                       cTTATCCTTGTTACTTGCGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAc                                                            <  1:655048/150‑1 (MQ=255)
                                                                                       cTTATCCTTGTTAATTGAGGGGGATGACTGGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAc                                                            <  1:663634/150‑1 (MQ=255)
                                                                                        ttATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAcc                                                           <  2:1113352/150‑1 (MQ=255)
                                                                                        ttATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAcc                                                           <  1:1817055/150‑1 (MQ=255)
                                                                                          aTCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCaa                                                         >  1:1934948/1‑150 (MQ=255)
                                                                                            ccTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAAt                                                       >  2:1161312/1‑150 (MQ=255)
                                                                                              ttGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCg                                                     >  1:148798/1‑150 (MQ=255)
                                                                                               tGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGa                                                    <  1:1810794/150‑1 (MQ=255)
                                                                                               tGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGa                                                    <  2:1991304/150‑1 (MQ=255)
                                                                                               tGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGa                                                    >  1:1078712/1‑150 (MQ=255)
                                                                                                gTTAATTGAGGGGGATGACTTGATTCTGCCATAAGAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGAc                                                   >  1:1079741/1‑150 (MQ=255)
                                                                                                gTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTTTGGAGGACCAAATCGAc                                                   >  1:701885/1‑150 (MQ=255)
                                                                                                  tAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCt                                                 >  2:955460/1‑150 (MQ=255)
                                                                                                  tAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCt                                                 >  1:675274/1‑150 (MQ=255)
                                                                                                  tAATTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACGAGACGTGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCt                                                 <  2:1639812/150‑1 (MQ=255)
                                                                                                    aTTGAGGTGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCAAGATCAGGTGTATCACTGAATAATAACATCGCCATTTGGCTGTTTCGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCa                                               <  1:203947/150‑1 (MQ=255)
                                                                                                    aTTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCa                                               <  1:7647/150‑1 (MQ=255)
                                                                                                      tGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCAtt                                             >  1:1062340/1‑150 (MQ=255)
                                                                                                       gAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGCCCGCATTc                                            >  1:798750/1‑150 (MQ=255)
                                                                                                        aGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCa                                           <  1:1054649/150‑1 (MQ=255)
                                                                                                                gACTTGATTCTGCCATAAATAAGCCCCGACGAGACGGGGCTAAATCATGATCCGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCggg                                   <  2:1679806/150‑1 (MQ=255)
                                                                                                                  cTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGCCTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGt                                 >  1:1228813/1‑150 (MQ=255)
                                                                                                                      aTTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCt                             >  1:1131976/1‑150 (MQ=255)
                                                                                                                           gCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                        <  2:771582/150‑1 (MQ=255)
                                                                                                                           gCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                        <  1:1341455/150‑1 (MQ=255)
                                                                                                                           gCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                        >  2:1846864/1‑150 (MQ=255)
                                                                                                                           gCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGATCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                        >  2:1770741/1‑150 (MQ=255)
                                                                                                                            ccATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGa                       >  2:751321/1‑150 (MQ=255)
                                                                                                                              aTAAAAACGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGc                     <  2:1466332/150‑1 (MQ=255)
                                                                                                                              aTAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGc                     >  1:1299813/1‑150 (MQ=255)
                                                                                                                               tAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGCCCAAAGCGCCCTCATTCAGGCGCCGGGTATCTGCCGGAGCg                    >  2:839160/1‑150 (MQ=255)
                                                                                                                                aaaaaaGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGt                   >  2:1024274/1‑150 (MQ=255)
                                                                                                                                aaaaaaGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTACACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGt                   >  2:307392/1‑150 (MQ=255)
                                                                                                                                 atttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGtt                  >  1:635369/5‑150 (MQ=255)
                                                                                                                                 atttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGtt                  >  2:1086708/5‑150 (MQ=255)
                                                                                                                                  aaaaGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTa                 >  2:1400025/1‑150 (MQ=255)
                                                                                                                                     aGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAAcc              <  1:1896339/150‑1 (MQ=255)
                                                                                                                                         ccGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCaa          <  2:1718198/150‑1 (MQ=255)
                                                                                                                                          cgacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTACCCGCAat         >  2:494160/1‑150 (MQ=255)
                                                                                                                                             cgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgac      <  2:1047313/150‑1 (MQ=255)
                                                                                                                                               cgaCGGGGCTAAATCATGATCCGGGGTTTCACTGAATACTAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacat    <  2:1355850/149‑1 (MQ=255)
                                                                                                                                               agaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacat    >  1:467082/1‑150 (MQ=255)
                                                                                                                                                gaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAGTAACATCGCCATTTGGCTGGTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacatg   <  1:571162/150‑1 (MQ=255)
                                                                                                                                                gaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacatg   >  2:1698327/1‑150 (MQ=255)
                                                                                                                                                gaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacatg   >  2:616200/1‑150 (MQ=255)
                                                                                                                                                 aCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacatga  <  1:559707/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACATGA  >  NC_000913/2411268‑2411562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: