Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,144 G→C 5.5% A32G (GCC→GGC)  purF ← amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,1440GC 5.5% 395.2 / 13.0 128A32G (GCC→GGC) purFamidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (63/58);  new base C (3/4);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 135 total aligned reads displayed.

TGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAA  >  NC_000913/2429995‑2430292
                                                                                                                                                     |                                                                                                                                                    
tGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCcgg                                                                                                                                                      <  2:273750/150‑1 (MQ=255)
tGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCcgg                                                                                                                                                      >  1:1357713/1‑150 (MQ=255)
 ggAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCcggc                                                                                                                                                     >  2:1375575/1‑150 (MQ=255)
   aGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCcggcgg                                                                                                                                                   >  1:1186860/1‑150 (MQ=255)
    gCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCggtggc                                                                                                                                                  >  2:284311/1‑150 (MQ=255)
    gCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCCTACGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCAggcggc                                                                                                                                                  >  1:233190/1‑150 (MQ=255)
     cTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGCCGGCa                                                                                                                                                 <  1:1095009/150‑1 (MQ=255)
      tGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCAt                                                                                                                                                >  2:1312907/1‑150 (MQ=255)
       gCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATc                                                                                                                                               >  2:1697316/1‑150 (MQ=255)
        ccAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATcc                                                                                                                                              <  1:689093/150‑1 (MQ=255)
        ccAGCCGTGGGGTAACGCACATGACAAATGCCCATATTGCCCTGCAAAAGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTGCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATcc                                                                                                                                              <  1:158261/150‑1 (MQ=255)
          aGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTg                                                                                                                                            <  2:19694/150‑1 (MQ=255)
            ccGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGCCGGCATcatcac                                                                                                                                          <  2:1848665/150‑6 (MQ=255)
            ccGTGGGGTAACGCACAAGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGCCGGCATcatcac                                                                                                                                          <  1:392949/150‑6 (MQ=255)
               tGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACcgc                                                                                                                                       >  1:1623492/1‑150 (MQ=255)
                  ggTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGAt                                                                                                                                    >  1:577364/1‑150 (MQ=255)
                     aaCGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGcg                                                                                                                                 >  2:1211108/1‑149 (MQ=255)
                       cGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGa                                                                                                                               >  2:899240/1‑150 (MQ=255)
                         cacaTGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAg                                                                                                                             <  1:1710107/150‑1 (MQ=255)
                         cacaTGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAg                                                                                                                             <  2:519789/150‑1 (MQ=255)
                          acaTGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGc                                                                                                                            <  2:703368/150‑1 (MQ=255)
                                ccAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTGTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGtt                                                                                                                      >  1:1219754/1‑150 (MQ=255)
                                      cccATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGca                                                                                                                >  2:120358/1‑150 (MQ=255)
                                             tGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCCCCAGCCCGTTCGCTTTACGCAAACGGAAGCCGGTATTGGCCGCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATaaa                                                                                                         >  1:224062/1‑150 (MQ=255)
                                              gCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCATGACCGCGATGCTGAAGCACCGTTACGGCATAATAAAt                                                                                                        >  1:1226277/1‑150 (MQ=255)
                                                 cTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGa                                                                                                     >  1:459210/1‑150 (MQ=255)
                                                   gCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACt                                                                                                   >  1:432101/1‑150 (MQ=255)
                                                   gCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACt                                                                                                   <  2:937907/150‑1 (MQ=255)
                                                   gCAAACGCTGCATAGGGCGAGCTTCAAATACATCGCGCACCAGCCCGTTCGCTTTACGCATACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACt                                                                                                   <  1:370423/150‑1 (MQ=255)
                                                     aaaCGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTgg                                                                                                 >  2:145572/1‑150 (MQ=255)
                                                      aaCGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGt                                                                                                >  1:1420799/1‑150 (MQ=255)
                                                      aaCGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGt                                                                                                <  1:2001486/150‑1 (MQ=255)
                                                            gCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCg                                                                                          >  2:1745695/1‑150 (MQ=255)
                                                              atatGGCGAGCTTCAAAAACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGCAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCTCGACGCTGAAGCACCGTTAAGGCATCATAAATCTACTGGTTAACCGGc                                                                                        >  2:1813466/1‑150 (MQ=255)
                                                                atGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGTGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGccg                                                                                      <  1:284311/150‑3 (MQ=255)
                                                                 tGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATa                                                                                     >  1:861154/1‑150 (MQ=255)
                                                                  ggCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATaa                                                                                    >  2:434227/1‑150 (MQ=255)
                                                                   gCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATTCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGAATCATAAATCGACTGGTTAACCGGCATAac                                                                                   >  1:330478/1‑150 (MQ=255)
                                                                   gCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCAGAac                                                                                   >  1:1036157/1‑150 (MQ=255)
                                                                       gCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCg                                                                               <  1:222019/150‑1 (MQ=255)
                                                                       gCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCg                                                                               >  2:854945/1‑150 (MQ=255)
                                                                        cTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCgg                                                                              >  2:40498/1‑150 (MQ=255)
                                                                        cTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGAAGTAATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCgg                                                                              >  2:1195936/1‑150 (MQ=255)
                                                                          tCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCggag                                                                            >  2:1278121/1‑148 (MQ=255)
                                                                           cAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGa                                                                           >  2:642326/1‑150 (MQ=255)
                                                                           cAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGa                                                                           >  1:1188208/1‑150 (MQ=255)
                                                                            aaaTACATCGCTCACCAGCCCGTTCGCTTTACGCAACCGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTATTGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCTAt                                                                          <  2:631068/150‑1 (MQ=255)
                                                                               tACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATAcc                                                                       <  2:2014229/150‑1 (MQ=255)
                                                                                   tCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACa                                                                   <  2:241498/150‑1 (MQ=255)
                                                                                          ccAGCCCGTTCGCTTTACGCAAACGGAAGCATTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGc                                                            <  2:1802546/150‑1 (MQ=255)
                                                                                          ccAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGc                                                            >  2:458657/1‑150 (MQ=255)
                                                                                            cttccGTTTGCGTAAAGCGAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGctc                                                          >  1:38232/4‑148 (MQ=255)
                                                                                            aGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGcac                                                          >  1:958952/1‑150 (MQ=255)
                                                                                             ttccGTTTGCGTAAAGCGAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGcaca                                                         >  1:1920856/3‑150 (MQ=255)
                                                                                               ccGTTTGCGTAAAGCGAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATa                                                       >  1:878613/1‑150 (MQ=255)
                                                                                                cGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGTTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATAc                                                      <  1:342578/150‑1 (MQ=255)
                                                                                                cGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATAc                                                      >  2:966222/1‑150 (MQ=255)
                                                                                                    cGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCt                                                  >  2:729891/1‑150 (MQ=255)
                                                                                                     gCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACAGGCATAACACCGGCGATACCGACAATACCGCACATACGTCtt                                                 >  1:964435/1‑150 (MQ=255)
                                                                                                      cTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCttt                                                >  1:532226/1‑150 (MQ=255)
                                                                                                        ttACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCttttt                                              <  1:1413496/150‑1 (MQ=255)
                                                                                                          aCGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTcc                                            <  2:281873/150‑1 (MQ=255)
                                                                                                           cGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCt                                           >  1:1233008/1‑150 (MQ=255)
                                                                                                           cGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCt                                           >  2:2011297/1‑150 (MQ=255)
                                                                                                            gCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTc                                          >  2:402466/1‑150 (MQ=255)
                                                                                                               aaCGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGtt                                       >  2:456840/1‑150 (MQ=255)
                                                                                                               aaCGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGAGGCATCCTGACCGCGATGCGGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGtt                                       <  2:1871181/150‑1 (MQ=255)
                                                                                                                    aaGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGcc                                  <  1:1312907/150‑1 (MQ=255)
                                                                                                                           tATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACAtctc                           >  1:1804909/1‑150 (MQ=255)
                                                                                                                           tATTGGCAGCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACAtctc                           <  2:637047/150‑1 (MQ=255)
                                                                                                                             ttGGCATCTATGGTGATGATGCCGGCGTCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACATCTTTTTCCTCGTTAAGCCACATCTCag                         <  1:144636/150‑1 (MQ=255)
                                                                                                                             ttGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCag                         <  1:782369/150‑1 (MQ=255)
                                                                                                                             ttGGCAGCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCag                         <  1:1478488/150‑1 (MQ=255)
                                                                                                                                gCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGc                      <  1:899240/150‑1 (MQ=255)
                                                                                                                                     tATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTa                 <  1:609310/150‑1 (MQ=255)
                                                                                                                                      agggTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCTTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATTCGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTAc                <  1:997127/148‑1 (MQ=255)
                                                                                                                                       tGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGTAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACg               >  1:309436/1‑150 (MQ=255)
                                                                                                                                       tGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACg               <  2:233190/150‑1 (MQ=255)
                                                                                                                                        gttgatgaTGCCTGCGTCATCCTGACCGCGATGCTGAATCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGc              <  1:375748/148‑1 (MQ=255)
                                                                                                                                        ggTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGGATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGc              <  2:82934/150‑1 (MQ=255)
                                                                                                                                        ggTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGc              >  1:1528850/1‑150 (MQ=255)
                                                                                                                                          tgatgaTGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGAGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGctc            >  1:1148704/1‑150 (MQ=255)
                                                                                                                                          tcaggatgCCGCCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATACCACCGGCGATACCGACAATACCGCACATACGTCTTTTTACTCGTTAAGCCACATCTCAGAGCACTTACGctc            >  1:1316845/5‑150 (MQ=255)
                                                                                                                                           gatgatGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGctct           >  1:957776/1‑150 (MQ=255)
                                                                                                                                           caggatgCCGCCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGACTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGctct           >  2:745374/4‑150 (MQ=255)
                                                                                                                                            atgatgCCGGCGGCATCCTGACCGCGATGCTGATGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATTCCGACAATACCGCACATGCGTCTTGTTCCTCGTTAAGCCACATCTCAGAGCACTTACGcggtg          >  1:1079656/1‑146 (MQ=255)
                                                                                                                                             tgatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTgg         <  1:1441015/150‑1 (MQ=255)
                                                                                                                                             tgatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTgg         <  1:1786310/150‑1 (MQ=255)
                                                                                                                                             tgatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTgg         <  1:492128/150‑1 (MQ=255)
                                                                                                                                              gatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTggg        >  1:1014136/1‑150 (MQ=255)
                                                                                                                                              gatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTggg        <  2:1017084/150‑1 (MQ=255)
                                                                                                                                              gatgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTggg        >  1:1071382/1‑150 (MQ=255)
                                                                                                                                               atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACTGTTAAGGCTTCATAAATCGACTGGTTAACCGGCATATCACCGGCGATACCGACAATACCGCACATACGTCTTTATCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGc       >  1:345797/1‑150 (MQ=255)
                                                                                                                                               atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCCTAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGc       <  1:1002600/150‑1 (MQ=255)
                                                                                                                                               atgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGCTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGc       <  2:150107/150‑1 (MQ=255)
                                                                                                                                                tgCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGCTACCGACAATACCGCACATCCGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCa      >  1:1818008/1‑150 (MQ=255)
                                                                                                                                                 gCCGGCGGCATCCTGACCGCTATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCaa     >  2:1583703/1‑150 (MQ=255)
                                                                                                                                                 gCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTACCCGGCATAACACCGGCGCTACCGCCAATACCGCACATACGTCGTGTTCCTCGTTAAGCCACAGCTCAGCGCACGTACGCTCGGGGCaa     >  1:748522/1‑150 (MQ=255)
                                                                                                                                                  ccGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAg    <  1:729891/150‑1 (MQ=255)
                                                                                                                                                    ggcggcATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGaa  <  1:185627/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGAGCTGCCAGCCGTGGGGTAACGCACATGACCAATGCCCATATTGCCCTGCAAACGCTGCATATGGCGAGCTTCAAATACATCGCTCACCAGCCCGTTCGCTTTACGCAAACGGAAGCAGTTATTGGCATCTATGGTGATGATGCCGGCGGCATCCTGACCGCGATGCTGAAGCACCGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAA  >  NC_000913/2429995‑2430292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: