Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,731 T→G 5.6% intergenic (‑25/‑311) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7310TG 5.6% 376.9 / 9.7 124intergenic (‑25/‑311)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (71/46);  new base G (5/2);  total (76/48)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 124 total aligned reads displayed.

TTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512585‑2512877
                                                                                                                                                  |                                                                                                                                                  
ttGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCt                                                                                                                                                 <  1:872454/150‑1 (MQ=255)
 tGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATATTTCGTCATCTTGTTCCTCTAAAACATAGCCTTTGCTa                                                                                                                                                >  1:1320982/1‑150 (MQ=255)
 tGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTa                                                                                                                                                <  1:930522/150‑1 (MQ=255)
  ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                               >  1:52854/1‑150 (MQ=255)
     cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                            >  1:14201/1‑150 (MQ=255)
        aaaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCa                                                                                                                                         <  1:1141248/150‑1 (MQ=255)
         aaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCAt                                                                                                                                        >  2:1603835/1‑150 (MQ=255)
         aaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGttttag                                                                                                                                        <  2:1246131/150‑4 (MQ=255)
         aaGTTACAGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCAt                                                                                                                                        <  2:628216/150‑1 (MQ=255)
           gTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGc                                                                                                                                      <  1:1158613/150‑1 (MQ=255)
               ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTTTGTTTCATGCTATg                                                                                                                                  >  2:568641/1‑150 (MQ=255)
               ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                  <  1:1978905/150‑1 (MQ=255)
               ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                  <  2:183426/150‑1 (MQ=255)
               ccGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATg                                                                                                                                  >  2:498181/1‑150 (MQ=255)
                 gggATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGcc                                                                                                                                <  2:867828/150‑1 (MQ=255)
                      cGATATAACCAATCGCCTCAATGAACGCAGGTCCCAATAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAAAGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACg                                                                                                                           <  2:1242637/150‑1 (MQ=255)
                       gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                          >  1:1090129/1‑150 (MQ=255)
                          ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGACATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCACACGAGaa                                                                                                                       >  1:1576585/1‑150 (MQ=255)
                           taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCACACGAGAAt                                                                                                                      >  2:891822/1‑150 (MQ=255)
                           taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                      >  1:1558507/1‑150 (MQ=255)
                            aaCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATg                                                                                                                     >  2:72755/1‑150 (MQ=255)
                             aCCAATCGCCGCAATGACCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGa                                                                                                                    >  2:365081/1‑150 (MQ=255)
                              ccAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCCTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCCATGCCAAACGAGAATGAt                                                                                                                   >  1:1031917/1‑150 (MQ=255)
                                aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                 <  2:653930/150‑1 (MQ=255)
                                aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                 <  1:1159874/150‑1 (MQ=255)
                                aaTCGCCGCAATGAACGCAGGTACCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                 >  2:1805342/1‑150 (MQ=255)
                                 aTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTAt                                                                                                                >  1:454625/1‑150 (MQ=255)
                                  tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                               <  1:1301847/150‑1 (MQ=255)
                                    gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                             >  2:1917398/1‑150 (MQ=255)
                                    gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                             >  2:576042/1‑150 (MQ=255)
                                     ccgcAATGACCGCAGGTCCCATTAATGCGAGCCTAATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTAGTGCCTCTAAACCATAGCCTTTGCTATGTTTCCTGCTATGCCAAACGAGAATGATTATCaaa                                                                                                            >  2:1420102/1‑150 (MQ=255)
                                     ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCACCGCGATAGTTCGTCCTCTTGTGCCTCTAAAACATAGCCATTGCTATGTTTCATGCTATGCCAAACGAGAAAGCTTATCaaa                                                                                                            >  2:1236408/1‑150 (MQ=255)
                                     ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAATCGAGAATGATTATCaaa                                                                                                            >  2:1336271/1‑150 (MQ=255)
                                      cgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAt                                                                                                           <  2:1909251/150‑1 (MQ=255)
                                       gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCTCCCGTCCGCGGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATCTCCTTTGCTATGTTTCATGCGATGCCAAACGAGAATGATTATCAAAtt                                                                                                          >  1:317171/1‑150 (MQ=255)
                                         aaTGAACGCAGGTCCCATTTATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCTTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                        <  1:1665790/150‑1 (MQ=255)
                                           tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      >  2:532611/1‑150 (MQ=255)
                                            gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAttt                                                                                                     <  2:741557/150‑1 (MQ=255)
                                                gCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                 <  1:769920/150‑1 (MQ=255)
                                                 cAGGTCCCATTAATGCTAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGAGCCTCTAAAACATAGCCTATGCTATGTTTCATGATATTCCAAACGAGAATGATTATCAAATTCATTTAATTg                                                                                                >  2:1813581/1‑150 (MQ=255)
                                                 cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                >  2:1487961/1‑150 (MQ=255)
                                                  aGGTCCGATTAATGCGAGCCTGATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATgg                                                                                               <  1:619372/150‑1 (MQ=255)
                                                     tCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGAtt                                                                                            <  2:1840935/150‑1 (MQ=255)
                                                           tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                      >  2:824960/1‑150 (MQ=255)
                                                            aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTACTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                     <  1:406696/150‑1 (MQ=255)
                                                              tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                   <  2:942574/150‑1 (MQ=255)
                                                              tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                   >  2:1169237/1‑150 (MQ=255)
                                                              tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                   >  1:1829282/1‑150 (MQ=255)
                                                                  aGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                               <  2:1627611/150‑1 (MQ=255)
                                                                   gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTAAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTTCTATGTTTCATGCTATGCCAATCGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                              >  1:123712/1‑150 (MQ=255)
                                                                    ccTCATCTTGCGCTCCTCCCGTCATCTGCTAATCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATTTTTCATGCTATCCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTg                                                                             <  2:189726/150‑1 (MQ=255)
                                                                     ctaaTCTTGAGCGCAGCCCGTCCGCTGCTACGCTCAACGCGATAGTTCGTCAACTTGTGCCTATAAAACATAGCCTTTGCTATGTATCATGCTATGCCAAACGTGAATGATTTTCAAATTGATTTAAATGGATTGTGGTGATTTATCTGa                                                                            >  2:1055587/4‑150 (MQ=255)
                                                                     cTCATCTTGCGCGCAGCCCGTCCGCTGCTACTCTCAACGCGATAGGTCGTCATCTTGTGCCTCTAAAACATAGGCTTTGATATGTTTCATGCTATGCCAAAGGAGACTGATTATCAAATTCATTTAAATGGAATGTGGTGATTTCTCTGa                                                                            >  2:283829/1‑150 (MQ=255)
                                                                      tCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGAt                                                                           >  2:2002564/1‑150 (MQ=255)
                                                                       cATCTTTCTCGCAGCCCGTCCGCTGCTACTCTCAAAGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTAAAATGGATTGTGGTGATTTCTCTGATa                                                                          <  1:326314/150‑1 (MQ=255)
                                                                          cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                       >  2:1563071/1‑150 (MQ=255)
                                                                           ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTGATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTTTCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                      <  2:394378/150‑1 (MQ=255)
                                                                           ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGTTAGAcc                                                                      >  1:464064/1‑150 (MQ=255)
                                                                            tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                     >  2:7449/1‑150 (MQ=255)
                                                                                cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAACCCTCGCCTTTGCTATGTTTCATGCTATGCCAATCGAGACTGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                 >  1:95096/1‑150 (MQ=255)
                                                                                cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                 >  1:1728823/1‑150 (MQ=255)
                                                                                  ccgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGACTGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTa                                                               <  1:251243/150‑1 (MQ=255)
                                                                                    gccCTTCAGCTGCTACTCTCAACTCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTTCTATGTTTCATGCTATGACAAACGAGAATGATTATCAAATTCATTTAAATTGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             <  1:125954/150‑1 (MQ=255)
                                                                                    gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             >  1:1628357/1‑150 (MQ=255)
                                                                                    gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             <  2:690978/150‑1 (MQ=255)
                                                                                    gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             >  1:1654122/1‑150 (MQ=255)
                                                                                        gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTTATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         <  2:1674512/150‑1 (MQ=255)
                                                                                        gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         >  2:710335/1‑150 (MQ=255)
                                                                                        gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         >  2:1539587/1‑150 (MQ=255)
                                                                                             gtgctACTCTCTAAGCGATAGTTCGTCATCTTGAGACTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaa                                                    <  1:110280/149‑1 (MQ=255)
                                                                                                ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGa                                                 >  1:838325/1‑150 (MQ=255)
                                                                                                  aCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAtt                                               <  1:1806877/150‑1 (MQ=255)
                                                                                                  aCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAtt                                               >  2:650432/1‑150 (MQ=255)
                                                                                                   ctctCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtg                                              >  2:119991/1‑150 (MQ=255)
                                                                                                    tctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGGATTATGACTACGAAAAGATtga                                             >  2:1432776/1‑150 (MQ=255)
                                                                                                     ctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGTTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtgat                                            >  1:589256/1‑150 (MQ=255)
                                                                                                        aaCGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGAc                                         >  1:1962815/1‑150 (MQ=255)
                                                                                                          cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACtt                                       >  2:1045954/1‑150 (MQ=255)
                                                                                                            cgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGa                                     >  2:1645234/1‑150 (MQ=255)
                                                                                                               tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATTCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                  <  2:1226948/150‑1 (MQ=255)
                                                                                                                   tCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATCCTATGCCAAACGAGAATAATTTACAAATTCATTTAAATGGATTGTGGTGATTACTCTGAAAGACAAGAATTATAACTACGAAAACATTAATCACTTGAATtttat                              >  2:1481074/1‑150 (MQ=255)
                                                                                                                    cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                             >  2:1312461/1‑150 (MQ=255)
                                                                                                                     gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                            <  1:1168859/150‑1 (MQ=255)
                                                                                                                         tCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttat                        >  1:1555993/1‑150 (MQ=255)
                                                                                                                          cTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttatg                       >  1:657957/1‑150 (MQ=255)
                                                                                                                          attGTGCCTATAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAAGGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttatg                       <  1:47876/149‑1 (MQ=255)
                                                                                                                            tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                     >  1:1046750/1‑150 (MQ=255)
                                                                                                                             gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                    >  1:1980416/1‑150 (MQ=255)
                                                                                                                               gCCTCTATAACATAGCCTTTGCTATGTTTCTTGCTATGCCAAAAGATAATGTTTATCAAATTCTTTTAAATGGATTGTGGTGATTTCTCTTATAGACCAGTATTATGACTACGAAAAGATTGATGACTTTAATGTTATGGTTATGTTTaa                  <  1:182253/150‑1 (MQ=255)
                                                                                                                               gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                  >  1:402451/1‑150 (MQ=255)
                                                                                                                                 gcatgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                >  1:459545/6‑150 (MQ=255)
                                                                                                                                  catgaaaCATAGCAAAGGCTATGTTTCCTGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTTTGGTTATGTTTAATGt               >  1:1020537/5‑150 (MQ=255)
                                                                                                                                  catgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACGTGAATGTTATGGTTATGTTTAATGt               >  1:133979/5‑150 (MQ=255)
                                                                                                                                   ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              >  1:139046/1‑150 (MQ=255)
                                                                                                                                   ctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATgct              >  2:389939/1‑148 (MQ=255)
                                                                                                                                    tAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa             >  1:1796242/1‑150 (MQ=255)
                                                                                                                                     gaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg            >  1:1776480/2‑150 (MQ=255)
                                                                                                                                     aaaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg            <  1:1469731/150‑1 (MQ=255)
                                                                                                                                              ccTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAc   <  2:557196/150‑1 (MQ=255)
                                                                                                                                               cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa  >  1:1585846/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512585‑2512877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: