Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 242,344 C→G 5.5% P320P (CCG→CCC fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913242,3440CG 5.5% 453.2 / 15.5 145P320P (CCG→CCCfadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (70/67);  new base G (4/4);  total (74/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 149 total aligned reads displayed.

GGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCAT  >  NC_000913/242196‑242493
                                                                                                                                                    |                                                                                                                                                     
ggCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGCTGTATTCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCcccgcccg                                                                                                                                                      >  1:1216268/1‑150 (MQ=255)
ggCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTCCGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCg                                                                                                                                                      >  1:1248802/1‑150 (MQ=255)
 gCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCgg                                                                                                                                                     <  2:1162171/150‑1 (MQ=255)
 gCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACCAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCgg                                                                                                                                                     >  1:1159961/1‑150 (MQ=255)
  cACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCACATTCCACGCCCGGc                                                                                                                                                    >  2:420147/1‑150 (MQ=255)
  cACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGAAATCGATCGCCACGAAGACAACTTTACCGCGCGTCGGTACGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGc                                                                                                                                                    >  1:1812784/1‑150 (MQ=255)
    cTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgt                                                                                                                                                  <  1:820138/150‑1 (MQ=255)
      ccaccaGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtgg                                                                                                                                                >  1:746059/1‑150 (MQ=255)
        accaGCATCCGCCAGCCTTGCCCGGCCATATTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCgtggtg                                                                                                                                              <  1:515793/150‑1 (MQ=255)
         ccaGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTGCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGtggtgg                                                                                                                                             <  1:1305551/150‑1 (MQ=255)
           cgCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCGGGCgtggaaatt                                                                                                                                           <  1:688418/149‑6 (MQ=255)
           aGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCGGGCgtggaaatt                                                                                                                                           <  1:1880740/150‑6 (MQ=255)
             cATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCGGGCgtggaaattgg                                                                                                                                         <  2:1144675/150‑8 (MQ=255)
             cATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTgg                                                                                                                                         <  1:1751033/150‑1 (MQ=255)
             cATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTgg                                                                                                                                         <  2:1041757/150‑1 (MQ=255)
              aTCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTggg                                                                                                                                        <  2:1541546/150‑1 (MQ=255)
               tCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGCCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGa                                                                                                                                       >  2:399351/1‑150 (MQ=255)
                 cGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATc                                                                                                                                     <  2:3775/150‑1 (MQ=255)
                 cGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATc                                                                                                                                     <  1:1049053/150‑1 (MQ=255)
                      gCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAgcgc                                                                                                                                >  1:94545/1‑150 (MQ=255)
                        cTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGcac                                                                                                                              <  2:578449/150‑1 (MQ=255)
                          tGCCCGGCCCTTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGGGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAg                                                                                                                            <  2:1532803/150‑1 (MQ=255)
                          agCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAg                                                                                                                            >  1:1521878/2‑150 (MQ=255)
                            cccGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGt                                                                                                                          >  1:680276/1‑150 (MQ=255)
                            cccGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGt                                                                                                                          >  1:1115855/1‑150 (MQ=255)
                            cccGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGt                                                                                                                          >  1:454847/1‑150 (MQ=255)
                             ccGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTa                                                                                                                         >  2:1026847/1‑150 (MQ=255)
                                  cATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGcc                                                                                                                    <  2:148302/150‑1 (MQ=255)
                                  cATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGGCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGcc                                                                                                                    <  1:244705/150‑1 (MQ=255)
                                   aTTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCt                                                                                                                   <  1:1040153/150‑1 (MQ=255)
                                      ttCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTaaa                                                                                                                >  2:1318177/1‑150 (MQ=255)
                                        cGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAAtc                                                                                                              <  1:754077/150‑1 (MQ=255)
                                        cGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAAtc                                                                                                              <  2:1277116/150‑1 (MQ=255)
                                         ggCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGCCCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAAtct                                                                                                             >  2:1899580/1‑150 (MQ=255)
                                            ccgccgATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAAtcttct                                                                                                          <  1:1476075/150‑1 (MQ=255)
                                             cgccgATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTg                                                                                                         <  2:23675/150‑1 (MQ=255)
                                              gccgATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGc                                                                                                        <  2:1080715/150‑1 (MQ=255)
                                                 gatgatGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCAcc                                                                                                     >  2:333180/1‑150 (MQ=255)
                                                   tgatgTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGCTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCAccgc                                                                                                   >  2:550040/1‑150 (MQ=255)
                                                    gatgTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCAccgcc                                                                                                  >  2:196191/1‑150 (MQ=255)
                                                     atgTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCAccgccg                                                                                                 >  2:1191217/1‑150 (MQ=255)
                                                     atgTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCAccgccg                                                                                                 <  2:1350952/150‑1 (MQ=255)
                                                       gTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAg                                                                                               >  2:1681597/1‑150 (MQ=255)
                                                       gTAATCGACCGGCACGAAGCCATGATTACCGCGCGTGGGTCCGGTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGTCCAATTTCCACGCCCGTCGTGGTGGGTGGGATCAGCGCACAGGTAATGGCTAAATCTTCTGCACCGCCGAg                                                                                               <  1:1573525/150‑1 (MQ=255)
                                                        tAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGACCGGTACGTTCAGCGGGAAGTGGCGAAGACCAATATCCACGCCCGGCGAGGTGGTTGGGATCAGCGCACAGGTAATCACTACATAATCTGCACCGCCGAGt                                                                                              >  1:66122/1‑150 (MQ=255)
                                                          atcgatcgGCACGAAGACATCTTTACCGCGCTTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTaa                                                                                            <  2:1458497/150‑1 (MQ=255)
                                                          atcgatcgGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTaa                                                                                            >  1:2000154/1‑150 (MQ=255)
                                                             gatcgGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAAttt                                                                                         >  2:1840974/1‑150 (MQ=255)
                                                              atcgGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAAtttt                                                                                        >  2:1716134/1‑150 (MQ=255)
                                                                  gCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCg                                                                                    <  2:856408/150‑1 (MQ=255)
                                                                   cACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCgg                                                                                   <  2:1377703/150‑1 (MQ=255)
                                                                      gAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCTATTTCGACGCCCGGCGTGGTGGTTGGGTTCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTc                                                                                <  2:210794/150‑1 (MQ=255)
                                                                        aGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCgg                                                                              <  1:276316/150‑1 (MQ=255)
                                                                         gACATCTTTACCGCGCGGCGGTCCGGTCTGGAACGGTACGGTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGga                                                                             <  2:1090346/150‑1 (MQ=255)
                                                                           cATCTTTACCGCGCGTCTGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTATACCGGGACGgaga                                                                           >  2:1098484/1‑150 (MQ=255)
                                                                           cATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGgaga                                                                           >  1:810717/1‑150 (MQ=255)
                                                                           cATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGgaga                                                                           >  1:587097/1‑150 (MQ=255)
                                                                           cATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGgaga                                                                           >  2:795614/1‑150 (MQ=255)
                                                                              cTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGtt                                                                        >  2:527135/1‑150 (MQ=255)
                                                                              cTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGtt                                                                        >  2:1878581/1‑150 (MQ=255)
                                                                              cTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGtt                                                                        <  2:829645/150‑1 (MQ=255)
                                                                              cTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGtt                                                                        >  2:955314/1‑150 (MQ=255)
                                                                              cTTTACCGCGCGGCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGtt                                                                        <  2:471780/150‑1 (MQ=255)
                                                                               tttACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGttt                                                                       <  2:1521878/150‑1 (MQ=255)
                                                                                 tACCGCGCGTCGGTCCGTTCTGGATCGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTCAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTaa                                                                     >  1:94893/1‑150 (MQ=255)
                                                                                  aCCGCGCGTCGGTCCGTGCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTaaa                                                                    <  2:1720049/150‑1 (MQ=255)
                                                                                    cgcgcgTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTAGGGATCAGCGCACAGGTAATGCCTAAAACATCTGCACCGCCGAGTAATTTTTCCGGCTCGGAGAGTTTAAACg                                                                  >  2:1507428/1‑150 (MQ=255)
                                                                                      cgcgTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCTCAGGTATTGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGcc                                                                >  1:1092135/1‑150 (MQ=255)
                                                                                      cgcgTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGcc                                                                >  2:1827207/1‑150 (MQ=255)
                                                                                          tCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGcc                                                            >  1:1430761/1‑150 (MQ=255)
                                                                                               ccGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCCAGc                                                       >  1:29460/1‑150 (MQ=255)
                                                                                                cGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCa                                                      <  1:1000830/150‑1 (MQ=255)
                                                                                                  ttCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCTCCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACg                                                    >  2:865653/1‑150 (MQ=255)
                                                                                                    cTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCCGCGCACAGGTAATGCCTAAGTCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGt                                                  >  2:960158/1‑150 (MQ=255)
                                                                                                      ggAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGAACCGCCCAGTAATTTTTCCGGGTCGGAGAGTTTAAGCGCCAGCCCAAGCACGGTCg                                                >  2:282804/1‑150 (MQ=255)
                                                                                                                gTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTTGGATCATCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGc                                      <  2:878932/150‑1 (MQ=255)
                                                                                                                    aGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCAGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCACGCACGGTCGCAATCGGTGCAAGc                                  >  2:69165/1‑150 (MQ=255)
                                                                                                                      cGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGt                                <  2:706800/150‑1 (MQ=255)
                                                                                                                        ggAAGTGTCTACGAACTATTTCCTCGCCCGGCGTGGTGGGTGGGATCAGAGCTCATGTAATGCCTTAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCATCCCAAGCACGGTCGCAATCGGTGCCAGCGTaa                              <  1:1721831/150‑1 (MQ=255)
                                                                                                                          aaGTGTCGACGACCTATTTCCACGCCCGGCGTGGTTGTTTTGATCAGCGGAGAGGTAATGCCTAAATCTTCTTCACCGGCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGACAGCCCAAGCAAGGTCGCAATCGGTGCCAGAGTAATg                            <  1:368496/150‑1 (MQ=255)
                                                                                                                            gTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTa                          >  1:1679974/1‑150 (MQ=255)
                                                                                                                                    gacCAATTTCCAGGCCCGGGGTGGGGGTGGGTATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGt                  <  1:420147/150‑1 (MQ=255)
                                                                                                                                     acgaaTTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTATATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCTAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGtt                 <  1:352954/147‑1 (MQ=255)
                                                                                                                                     acCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGtt                 >  1:1632830/1‑150 (MQ=255)
                                                                                                                                     acCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGtt                 >  1:1312609/1‑150 (MQ=255)
                                                                                                                                      ccaaccaccACGCCGGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTc                >  2:36452/8‑150 (MQ=255)
                                                                                                                                      ccAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTc                >  1:1661363/1‑150 (MQ=255)
                                                                                                                                      ccAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTc                >  2:800144/1‑150 (MQ=255)
                                                                                                                                      ccAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTc                <  2:454847/150‑1 (MQ=255)
                                                                                                                                      ccAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTc                >  1:1653328/1‑150 (MQ=255)
                                                                                                                                       cAATTTCCACGCCCGGCGGGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTcc               <  1:1132041/150‑1 (MQ=255)
                                                                                                                                        aaTTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCa              <  1:1899580/150‑1 (MQ=255)
                                                                                                                                         accacaaCGCCGGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAg             >  1:1588792/7‑150 (MQ=255)
                                                                                                                                          tttCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAgg            <  1:967314/150‑1 (MQ=255)
                                                                                                                                          tttCCACGCCCGGAGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTTCTCCGCCGAGTAATTTTTCCGGGTCGTAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAgg            <  2:1965466/150‑1 (MQ=255)
                                                                                                                                             ccACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCACTCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCa         >  2:1667080/1‑150 (MQ=255)
                                                                                                                                             ccACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCa         >  2:584646/1‑150 (MQ=255)
                                                                                                                                              cACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAg        >  1:445211/1‑150 (MQ=255)
                                                                                                                                              cACGCCCGGCGTGGTGGTTGGGATCAGAGCACAGGTAAAGCCTATATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGAGTAATGTAGCGTTTGTTCCCGGTctg        >  2:319359/1‑148 (MQ=255)
                                                                                                                                                  cccGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCACGCCCGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGc    >  2:1998364/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACGTTCAGCGGGAAGTGGCGACGACCAATTTCCACGCCCGGCGTGGTGGTTGGGATCAGCGCACAGGTAATGCCTAAATCTTCTGCACCGCCGAGTAATTTTTCCGGGTCGGAGAGTTTAAACGCCAGCCCAAGCACGGTCGCAATCGGTGCCAGCGTAATGTAGCGTTTGTTCCAGGTCAGACGCAT  >  NC_000913/242196‑242493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: