Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,225 T→A 6.1% intergenic (+20/+32) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2250TA 6.1% 307.6 / 4.2 106intergenic (+20/+32)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (52/47);  new base A (4/3);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACC  >  NC_000913/2519081‑2519373
                                                                                                                                                |                                                                                                                                                    
aTCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtctc                                                                                                                                                 >  1:931687/1‑150 (MQ=255)
 tCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACTGCAGGATATTATCTcc                                                                                                                                                >  1:554029/1‑150 (MQ=255)
   gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                              >  2:381550/1‑150 (MQ=255)
     cAAATCATCAACGCGAGAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGcc                                                                                                                                            <  2:104622/150‑1 (MQ=255)
     aaaaTCATCAACGCGATAGATAATATGACAGAAATTGAGCAGTAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCACGTCTCGTTATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGcc                                                                                                                                            <  1:996834/149‑1 (MQ=255)
      aaaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCg                                                                                                                                           <  1:852280/150‑1 (MQ=255)
        atcctcAACGCGATAGATAATATGACAGACATTGAGCTGGAAAAAGTCGCGCAATTTTTGTCACGTGAATGAATTCGCAATTTCCTCGACCTTCTCGATATTGACGAATCAGCATAAATCAAACACGGCAGGAGATAATATCCTGCCGtt                                                                                                                                         <  1:169405/150‑1 (MQ=255)
        atcatcAACGCGATAGATAATATGACATCCATTGACCAGTAAAATGTCGCGCATTTTTTGTAACTTGAAGTAATTCGCAATTTCCTGGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                                                                         <  2:1453005/150‑1 (MQ=255)
        atcatcAACGCGATAGATAATATGACAGACATTGAGCAGTAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGtt                                                                                                                                         <  2:726271/150‑1 (MQ=255)
         tcatcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGttt                                                                                                                                        >  2:1484860/1‑150 (MQ=255)
          aatcaACGCTATATATAATATGACAGACATTTAGCAGGAAAAAGTCGCGAAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTa                                                                                                                                       <  2:1906612/149‑1 (MQ=255)
           atcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                      <  2:1928392/150‑3 (MQ=255)
             caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                    >  2:1553583/1‑150 (MQ=255)
             caACGCGATAGATAATATGACAGACATTGAGCAAGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTAGATATTGAAGAATCATCATAAATAAAAAACGGCAGTATATTATCTCATGACGTAAATCt                                                                                                                                    >  1:202733/1‑150 (MQ=255)
             caACGCGATAGATAAAATGAAAGACATTGAGCAGGACAAAGTCGCGCAATTTTTGTCACTTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGtttttta                                                                                                                                    <  1:1674136/150‑5 (MQ=255)
             caACGAGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAAAGGCAGGATATTATCTCCTGGCGTTTATCt                                                                                                                                    >  2:628196/1‑150 (MQ=255)
               aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                                  >  1:443832/1‑150 (MQ=255)
                  cgATAGATAATATGACAGACATTGATCAGTAAAAAGTCTCTCAATTTTTGTCACTTGAATGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                               <  1:1423418/150‑1 (MQ=255)
                  cgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGAGATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                               >  2:1818146/1‑150 (MQ=255)
                   gatagataTTATGACAGACATTGAGCCGGAAAAAGTCGCGCAATTTTTGACACTTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGGTATTATCTCCTGCCGTTTATCTTTTTac                                                                                                                              <  1:358639/150‑1 (MQ=255)
                    atagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTaca                                                                                                                             >  2:1810243/1‑150 (MQ=255)
                       gataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTTTCTCCTGCCGTTTATCTTTTTACACGc                                                                                                                          >  1:682814/1‑150 (MQ=255)
                       gataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                                                                          <  1:1846343/150‑1 (MQ=255)
                        ataataTGACAGCCATTGAGCAGCAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCt                                                                                                                         <  2:280071/150‑1 (MQ=255)
                        ataataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCt                                                                                                                         <  2:1223293/150‑1 (MQ=255)
                         taataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTa                                                                                                                        >  2:845957/1‑150 (MQ=255)
                           atatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAt                                                                                                                      >  1:1125238/1‑150 (MQ=255)
                               tacagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAcc                                                                                                                  <  1:1065011/149‑2 (MQ=255)
                                 cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgc                                                                                                                >  2:871459/1‑150 (MQ=255)
                                  agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                               <  1:1676826/150‑1 (MQ=255)
                                       ttGAGCAGGAAAAATTCTCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATCTGGCCGACTCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAttt                                                                                                          <  1:1541403/150‑1 (MQ=255)
                                       ttGAGCAGGAAAAAGTAGCGCAATTTTTGTCACGTGAAGGAATTAGCAATTTCCTCGCCCTTCTCGAGATTGACGAATCAGCATAAATAAAAAAACTCAGGATATTATCTCCTGCCGTTAATATTTTTACACCCTAATTACTGCTGAttt                                                                                                          >  2:887522/1‑150 (MQ=255)
                                         taccAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtttta                                                                                                        >  2:898195/4‑149 (MQ=255)
                                         gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                                        >  1:1999228/1‑150 (MQ=255)
                                         gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                                        >  1:499935/1‑150 (MQ=255)
                                           gCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATCTCCTCGCTCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCAGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgc                                                                                                      >  1:1466843/1‑150 (MQ=255)
                                              ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACTGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                                   >  2:813242/1‑150 (MQ=255)
                                                aaaaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                                                                 <  1:648624/150‑1 (MQ=255)
                                                  aaaGTTGCGCAATTTTTGTCACGTGAATGAATTCTCAATTTCATCTCCCGTCTCGATATTGACGAATCAGCATAATTAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAga                                                                                               <  1:183106/150‑2 (MQ=255)
                                                  aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                               <  2:1119414/150‑1 (MQ=255)
                                                    aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCaa                                                                                             <  2:1470368/150‑1 (MQ=255)
                                                       cgcgCATTTTTTGTCAAGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTTACGAATCATCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTAGCGTTCAGCAATaa                                                                                          <  2:485555/150‑1 (MQ=255)
                                                       cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGAATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa                                                                                          <  1:1703882/150‑1 (MQ=255)
                                                         cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                                        >  1:950816/1‑150 (MQ=255)
                                                           cAATTTTTGTCACGGGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTATTACAAGCTAATTAATGCTGATTTTCGCGTTCAGCAATAAAATc                                                                                      >  1:1115432/1‑150 (MQ=255)
                                                            aaTTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc                                                                                     <  2:1167436/150‑1 (MQ=255)
                                                             aTTTTTGTCACGTGATGGAATTCGCAATATACACGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGTCAGTAAATTAACTACTGCAGATTATCTTTTTCCACGATAATTAGTGCTGATTTTCGCGAGCATCAATAAAATcaa                                                                                    >  2:377100/1‑148 (MQ=255)
                                                                 ttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATACAgcgc                                                                                >  1:1927835/1‑150 (MQ=255)
                                                                   gTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtt                                                                              >  2:1746534/1‑150 (MQ=255)
                                                                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATTTTGACGAATCAGCATAAATAAAAAACTGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttg                                                                            <  1:21819/150‑1 (MQ=255)
                                                                     cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGAGTTAAGCAATAAAATCCAGCGCtttt                                                                            >  1:566458/1‑149 (MQ=255)
                                                                       cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                          <  1:1601363/150‑1 (MQ=255)
                                                                       cGTGAAGGAATTCGCAATTTCCTAGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                          >  2:110345/1‑150 (MQ=255)
                                                                          gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGaa                                                                       >  2:1588979/1‑149 (MQ=255)
                                                                          gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                       <  2:1492655/150‑1 (MQ=255)
                                                                                aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                                 <  2:520933/150‑1 (MQ=255)
                                                                                aTTCGCAATTTCCTCGCCAGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                                 >  2:1958214/1‑150 (MQ=255)
                                                                                   cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTTACTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                              >  1:1337753/1‑150 (MQ=255)
                                                                                   cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                              <  1:1039308/150‑1 (MQ=255)
                                                                                   cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                              <  2:1997681/150‑1 (MQ=255)
                                                                                   cGCAATTTCCTCGCCCGTCTCGATATTGAATAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                              <  1:757979/150‑1 (MQ=255)
                                                                                     cAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAAAGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc                                                            >  1:106661/1‑150 (MQ=255)
                                                                                       aTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCTCTTTGTTGATACGCTCGATACTg                                                          >  2:1846357/1‑150 (MQ=255)
                                                                                          tCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTAAACGCTAATTACTGCTGATTTTCGCGTTAAGCAATAAAATCCAGCGCTTTGTTGATACGCACGATACTGCgg                                                       >  1:637253/1‑150 (MQ=255)
                                                                                            cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATACTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                     >  2:619169/1‑150 (MQ=255)
                                                                                            cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATACTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                     >  2:1571055/1‑150 (MQ=255)
                                                                                                 ccGTCTCGATATTGACGAATCATCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc                                                <  1:108951/150‑1 (MQ=255)
                                                                                                 ccGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc                                                <  1:460397/150‑1 (MQ=255)
                                                                                                 ccGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCACGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc                                                <  2:259187/150‑1 (MQ=255)
                                                                                                  cGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAcc                                               <  1:1378926/150‑1 (MQ=255)
                                                                                                  cGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAcc                                               <  2:1464962/150‑1 (MQ=255)
                                                                                                  cGTCTCGATATTGACGAAACAGCATAAATAAAAAACGGCATGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAcc                                               <  1:1240326/150‑1 (MQ=255)
                                                                                                     ctcGATATTGACGAATCGTGATAAATAAAAACCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAAt                                            <  2:125351/150‑1 (MQ=255)
                                                                                                       cGATATTGACGAATCATCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                          <  1:845957/150‑1 (MQ=255)
                                                                                                         atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg                                        <  1:381550/150‑1 (MQ=255)
                                                                                                            ttGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGa                                     >  1:1923014/1‑150 (MQ=255)
                                                                                                             tGACGAATCAGCATAAATAAAAAACGGCAGGATATTTTCTCCTGCCGTTTATCTTGTTACACGCTAATTACTGCTGATTTTCGCGTTGAGCAATAACATCCAGAGCTTTGTTGATACGCTCGATCCTGCGGGTCTTACGAATGGCGTGaa                                    >  2:1228944/1‑150 (MQ=255)
                                                                                                                 gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGt                                >  1:428537/1‑150 (MQ=255)
                                                                                                                      aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCATGAACGGTAACAt                           <  1:515577/150‑1 (MQ=255)
                                                                                                                        cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCATTGTTGATACGCTCTATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATcc                         >  2:1264700/1‑150 (MQ=255)
                                                                                                                         ataaataaAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAGTTGCGTGAACGGTAACATCCa                        >  2:1468259/1‑150 (MQ=255)
                                                                                                                           aaataaaAAACGGCAGGTTATTATCTCCTGCCGTTTATCTTTTTACACTCTAATAACTGCTGATTATCGCGTTCAGCAATAAAATCCAGCGCTTTGATGATACGCTCGAAACTGCGGGTCTTACCAATTGAGTGAACGGTAACATCCatt                      >  1:1430992/1‑148 (MQ=255)
                                                                                                                            aataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTTTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTg                     >  2:370234/1‑150 (MQ=255)
                                                                                                                            aaagataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTg                     >  2:1158084/7‑150 (MQ=255)
                                                                                                                             ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc                    >  2:552211/1‑150 (MQ=255)
                                                                                                                             ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc                    >  2:1144398/1‑150 (MQ=255)
                                                                                                                              taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTAGCGTTCAGCAATAAAATCCAGCGCTTTCTTGATACGATCGAAACTGCGTGTCTTACCAATTTCGTGAACGGTAACATCCAGTGCt                   >  1:793739/1‑150 (MQ=255)
                                                                                                                               aaaaatCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg                  <  1:110345/150‑1 (MQ=255)
                                                                                                                               aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAGTGCTGATTTTGGCGTTCAGCAATAAAATCCATCGGATTGTTAATACCCTCGATACTGCTGTTCTTAAAAATTGCGTGAACTGTAAGATCCAGAGCTg                  >  2:1246108/1‑150 (MQ=255)
                                                                                                                               aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg                  >  2:1127517/1‑150 (MQ=255)
                                                                                                                                aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg                 <  1:1746534/150‑1 (MQ=255)
                                                                                                                                 aaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGga                >  1:1905524/1‑150 (MQ=255)
                                                                                                                                  aaaCTGCAGGATATTATCTACTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAATTCCAGCGCTTTTTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag               <  1:628196/150‑1 (MQ=255)
                                                                                                                                   aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAACTCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga              >  1:1881517/1‑150 (MQ=255)
                                                                                                                                   aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga              >  1:930145/1‑150 (MQ=255)
                                                                                                                                   aaCGGCAGGAGATAATATCCTGCCGTTTATCATTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAAAAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAACAATTGCGTGAACGGTAACATCCAGAGCTGgaga              >  1:1738787/1‑150 (MQ=255)
                                                                                                                                      ggCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAAACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTg           >  2:334025/1‑150 (MQ=255)
                                                                                                                                       gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc          >  2:89963/1‑150 (MQ=255)
                                                                                                                                              attatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCAc   <  2:1798256/150‑1 (MQ=255)
                                                                                                                                              attatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCAc   <  1:1305853/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCACC  >  NC_000913/2519081‑2519373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: