Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,344 C→T 6.9% intergenic (+16/+249) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,3440CT 6.9% 234.4 / 5.3 87intergenic (+16/+249)lysV/xapRtRNA‑Lys/transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base C (32/48);  new base T (3/3);  total (35/52)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

CACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/2521197‑2521493
                                                                                                                                                   |                                                                                                                                                     
cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTAGGGTCGATAGATCATTAGGTATAGCAGTTGACTTTTAATCAATTGGTCTCAGCTAAGAATCCTGCCCGACACACAAAAGAAAAAAAGCGcccaa                                                                                                                                                     >  2:194143/1‑148 (MQ=14)
              ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                                                                                                       >  1:197266/1‑150 (MQ=255)
              ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                                                                                                       <  2:539421/150‑1 (MQ=255)
              ggaggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                                                                                                       <  1:1259478/150‑1 (MQ=255)
               gaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTAc                                                                                                                                      <  1:1067801/150‑1 (MQ=255)
               gaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTAc                                                                                                                                      <  1:1233966/150‑1 (MQ=255)
                tgggggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCTTTTTTAca                                                                                                                                     <  2:619658/149‑2 (MQ=255)
                    ggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCTTTTTTAcattgg                                                                                                                                 <  1:1042416/150‑6 (MQ=255)
                      tCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGc                                                                                                                               <  1:170709/150‑1 (MQ=255)
                      tCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGc                                                                                                                               <  1:672577/150‑1 (MQ=255)
                       cggcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCg                                                                                                                              <  2:733922/150‑1 (MQ=255)
                       cggcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCg                                                                                                                              <  2:1421211/150‑1 (MQ=255)
                          cggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATa                                                                                                                           >  2:1221772/1‑150 (MQ=255)
                          cggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTATTACTATCTGCGATa                                                                                                                           >  2:1012539/1‑150 (MQ=255)
                              tctaTCACGTCATAACCCACCTCTTCGGGTCGTTTTCTCTGTTGGTAGAGCAGTTGACTTTTAATCAATTGTTCGCAGGTTCGAATCATGCACGAACCACCAATGTAAAAAAGCTCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCa                                                                                                                       <  1:1535268/147‑1 (MQ=14)
                              tCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCa                                                                                                                       <  1:522779/150‑1 (MQ=255)
                               cGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaa                                                                                                                      >  2:1174526/1‑150 (MQ=255)
                                gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                                                                                                     >  1:963011/1‑150 (MQ=255)
                                gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                                                                                                     >  1:1664804/1‑150 (MQ=255)
                                 aTCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAg                                                                                                                    >  1:178584/1‑150 (MQ=255)
                                   cccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAt                                                                                                                  <  1:587894/150‑1 (MQ=255)
                                     cGTCATCACCCACCTCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTc                                                                                                                <  2:66435/150‑1 (MQ=255)
                                       tcatcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGa                                                                                                              <  2:575511/150‑1 (MQ=255)
                                         atcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTATAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAc                                                                                                            <  2:1450925/150‑1 (MQ=255)
                                          tcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAcc                                                                                                           <  1:1458286/150‑1 (MQ=255)
                                             cccaccACTTGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                                                                                                        <  1:809437/150‑1 (MQ=255)
                                             cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                                                                                                        <  1:786474/150‑1 (MQ=40)
                                               caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGATTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCTATACTCAAAGATTCGAACCTgcag                                                                                                      <  1:840836/150‑1 (MQ=255)
                                               caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcag                                                                                                      <  1:214213/150‑1 (MQ=39)
                                                accacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcagc                                                                                                     <  2:1409655/150‑1 (MQ=38)
                                                 ccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcagca                                                                                                    <  2:50245/150‑1 (MQ=38)
                                                    cTTCGGGGCGTTACCGCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTGCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                 <  1:1264031/150‑1 (MQ=11)
                                                      tCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt                                                                                               <  1:127502/150‑1 (MQ=33)
                                                        gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga                                                                                             <  1:469848/150‑1 (MQ=32)
                                                        gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga                                                                                             <  2:790602/150‑1 (MQ=32)
                                                            cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGACCCTGCAGCAGGGttgagttg                                                                                         >  2:53674/1‑150 (MQ=17)
                                                            cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg                                                                                         <  2:536267/150‑1 (MQ=32)
                                                                   tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg                                                                                  >  1:1222029/1‑150 (MQ=32)
                                                                    cAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGc                                                                                 >  2:354584/1‑150 (MQ=17)
                                                                      gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGa                                                                               >  1:1873962/1‑150 (MQ=32)
                                                                        tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa                                                                             <  2:1663062/150‑1 (MQ=32)
                                                                         ggTAGAGCAGTTGACTTTTAATCAATTGCTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaac                                                                            <  1:194143/150‑1 (MQ=17)
                                                                          gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaaca                                                                           >  2:1799498/1‑150 (MQ=32)
                                                                              cgCAGTTGACTTTTACTCACTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTCAAATCGCGCCCTCAAGGCGCTTTTTTACTAGCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTGGAGCGCAGCGAAACAACgg                                                                       <  1:46557/149‑1 (MQ=11)
                                                                               gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGa                                                                      >  1:174082/1‑150 (MQ=32)
                                                                                 aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGCCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAGTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGc                                                                    >  1:1146227/1‑150 (MQ=17)
                                                                                 aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGc                                                                    >  1:1332627/1‑150 (MQ=32)
                                                                                      aCTTTTAATCAATTGGTCGCAGGTTCGAAGCCTGCACGACCCCCCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTc                                                               >  1:1099861/1‑150 (MQ=17)
                                                                                       cTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcg                                                              <  1:773434/150‑1 (MQ=32)
                                                                                        ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                             >  1:1402517/1‑150 (MQ=32)
                                                                                          ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                           <  1:1039987/150‑1 (MQ=32)
                                                                                          ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                           >  1:689757/1‑150 (MQ=32)
                                                                                           tAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                          <  2:595746/150‑1 (MQ=32)
                                                                                           tAATCAATTGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGAAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCTATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                          <  2:1702427/150‑1 (MQ=12)
                                                                                               cAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAGGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACg                                                      >  1:1128011/1‑150 (MQ=17)
                                                                                                 aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTACAACAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCACAGATTCGCACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGc                                                    <  1:292069/150‑1 (MQ=255)
                                                                                                  ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc                                                   <  2:526768/150‑1 (MQ=32)
                                                                                                    ggTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAAGGCCCg                                                 >  2:691657/1‑150 (MQ=17)
                                                                                                     gTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGa                                                >  2:576593/1‑150 (MQ=32)
                                                                                                     gTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCGGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCAGa                                                >  1:946079/1‑150 (MQ=17)
                                                                                                         cAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAggg                                            <  2:19306/150‑1 (MQ=32)
                                                                                                         cAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGAACATGCAGCAGGTTTGAGTTGAGAGCAGCGAAACAACGGAGCCGCTAGCGGCGACGGCCCGAAggg                                            >  2:1107784/1‑150 (MQ=255)
                                                                                                          aGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc                                           <  2:98491/150‑1 (MQ=32)
                                                                                                             ttCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag                                        <  2:1222029/150‑1 (MQ=32)
                                                                                                               cGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgagcg                                      <  2:1861515/150‑1 (MQ=32)
                                                                                                                   tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  >  1:671642/1‑150 (MQ=32)
                                                                                                                   cccTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  <  1:506334/149‑1 (MQ=17)
                                                                                                                   accTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  <  2:342492/149‑1 (MQ=17)
                                                                                                                   accTGCACGACCCACCAATGAAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGAAACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  <  1:743872/149‑1 (MQ=17)
                                                                                                                    ccTGCACGACCCACCAATTTAAAAACGCGCCATAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAAAAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcg                                 <  2:531797/150‑1 (MQ=255)
                                                                                                                      cgCACGACCCACCAATGTAAAAACGCGCCCTACAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAg                               <  1:81144/149‑1 (MQ=17)
                                                                                                                             cccACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt                        >  2:266949/1‑150 (MQ=32)
                                                                                                                              ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACCCAAAGATTCGAACCTGCAGCAGGTTGGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTg                       >  1:758101/1‑150 (MQ=255)
                                                                                                                                cagatagTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCATGCa                     >  2:1738882/7‑150 (MQ=11)
                                                                                                                                accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                     <  2:854651/150‑1 (MQ=32)
                                                                                                                                 aaaaTTTAAAAAAGCGCATTAAAGGCGTTTTTTTATTATTTGCGATACTAAAATTTTGTACGCTGAAGTTTGTTTTTGTTGTGTGATGGTAAAAAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc                    <  2:1760964/148‑1 (MQ=2)
                                                                                                                                    aTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAc                 >  2:1096953/1‑150 (MQ=32)
                                                                                                                                      gTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc               >  1:1417776/1‑150 (MQ=12)
                                                                                                                                      gTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAcccc              >  1:456818/1‑149 (MQ=11)
                                                                                                                                       tAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGTTACTCAACGATTCGACCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca              <  1:688001/150‑1 (MQ=12)
                                                                                                                                       tAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGccccc              >  2:2009747/1‑149 (MQ=17)
                                                                                                                                        aaaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac             >  1:1313404/1‑150 (MQ=32)
                                                                                                                                          aaacGCGCCCTAACGGCGATTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca           <  2:197266/150‑1 (MQ=11)
                                                                                                                                           aaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGTTACTCATAGATTCGAACCTGAAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGTCGCGAAGGGCGAGCGATGCGAGTCATCCTGCCCGGCCCGCCaa          >  1:130483/1‑150 (MQ=11)
                                                                                                                                           aaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa          >  1:1174071/1‑150 (MQ=32)
                                                                                                                                            aaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt         <  1:614686/150‑1 (MQ=32)
                                                                                                                                             cgcgcCCTAAAGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGAACCTGCAGCAGGTTTTAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCTAAGCGAGTCATCCTGCACGACCCACCAATg        <  1:162503/149‑1 (MQ=12)
                                                                                                                                               cgcCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTa      >  2:162138/1‑150 (MQ=32)
                                                                                                                                                 cccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaa    >  2:1027760/1‑150 (MQ=32)
                                                                                                                                                 cccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaa    >  1:498837/1‑150 (MQ=32)
                                                                                                                                                   cTAAACGCGCTTTTTTACTATCTGCGATACTCAAAGCTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa  <  2:560761/150‑1 (MQ=17)
                                                                                                                                                   |                                                                                                                                                     
CACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/2521197‑2521493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: