Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,550,574 C→G 5.6% intergenic (‑29/+67) yfeX ← / ← yfeY porphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,550,5740CG 5.6% 501.7 / 18.0 160intergenic (‑29/+67)yfeX/yfeYporphyrinogen oxidase, cytoplasmic/RpoE‑regulated lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (77/73);  new base G (5/4);  total (83/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

Only 100 of 161 total aligned reads displayed.

GTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACGCC  >  NC_000913/2550425‑2550720
                                                                                                                                                     |                                                                                                                                                  
gTTAGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTAAACTTCCCCTTTCACGTTTGCTTCGATCAAATTCGCCGCTCGGCAATGTACTGTCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGc                                                                                                                                                    <  1:376247/150‑1 (MQ=255)
    gTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATc                                                                                                                                                >  1:718289/1‑150 (MQ=255)
     tCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATcc                                                                                                                                               >  1:1068749/1‑150 (MQ=255)
        gctaaTGTTTTACTGGCCGCACGCAGGGCGGCAACTTCCCCTTTCACGTTGGCTTCGATCCTAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTg                                                                                                                                            <  2:698699/147‑1 (MQ=255)
         cAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTgg                                                                                                                                           <  1:1668609/150‑1 (MQ=255)
          aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGc                                                                                                                                          <  1:924253/150‑1 (MQ=255)
          aaaTGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGc                                                                                                                                          <  2:243710/150‑1 (MQ=255)
             tGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAtt                                                                                                                                       <  2:1760530/150‑1 (MQ=255)
                tttACTGGCCGCTCGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAACAATGCCACGGATTGCGTGGCattatt                                                                                                                                    <  1:1940553/150‑1 (MQ=255)
                tttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGGATTGCGTGGCattatt                                                                                                                                    <  2:384387/150‑1 (MQ=255)
                 ttACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAgtgttg                                                                                                                                   >  2:1529286/1‑150 (MQ=255)
                  tACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGa                                                                                                                                  <  2:83648/150‑1 (MQ=255)
                   aCTGGCCGCACGCAGGGCGTCAACTTCACCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGAAATGTTATGGCAAAATGCCACTCTGAACCTGAGACCTTGTTCCTCCTGAAAATAATAATGCCACTCAATCCGTGGCATTGTTGAg                                                                                                                                 <  1:1973122/150‑1 (MQ=255)
                    cTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgc                                                                                                                                <  2:16157/150‑1 (MQ=255)
                     tGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgcg                                                                                                                               >  1:1577260/1‑150 (MQ=255)
                      ggCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAACTGCCTCTCTGACCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACCGATTGCGTGGCattattattttc                                                                                                                              <  1:292351/150‑7 (MQ=255)
                           cacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGCGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAttt                                                                                                                         >  1:831695/1‑150 (MQ=255)
                           cacgcaGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAttt                                                                                                                         <  2:486943/150‑1 (MQ=255)
                             cgcaGGGCGTCAACTTCCCCTTTCACGTTGGCATCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTa                                                                                                                       <  2:2014943/150‑1 (MQ=255)
                               caGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACg                                                                                                                     <  2:1090067/150‑1 (MQ=255)
                                   gCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGtttt                                                                                                                 >  1:316898/1‑150 (MQ=255)
                                   gCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtt                                                                                                                 >  2:1187408/1‑150 (MQ=255)
                                   gCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtt                                                                                                                 >  1:1949549/1‑150 (MQ=255)
                                     gTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGtttt                                                                                                               >  2:1889244/1‑150 (MQ=255)
                                          cTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgc                                                                                                          >  2:502993/1‑150 (MQ=255)
                                          cTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgc                                                                                                          >  1:1646397/1‑150 (MQ=255)
                                           ttCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTcgcg                                                                                                         <  2:483110/150‑1 (MQ=255)
                                             ccccTTTCACGTTGGCTTCGATCAAAATAGCCGAGCGGCAATGTTCTGGCAAAATGCCAATCTGAACCTGAGACATTGTTCCTCCTGAAAATAATATTGCCACGCAATCCGTGGCATTGTTGAGAGCTATTTTACGTGTTTTTTCtcggc                                                                                                       >  2:69653/1‑150 (MQ=255)
                                                    cACGGTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGtt                                                                                                <  2:1676423/150‑1 (MQ=255)
                                                      cGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTg                                                                                              >  1:1938141/1‑150 (MQ=255)
                                                      cGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTg                                                                                              >  1:712347/1‑150 (MQ=255)
                                                       gTTGGCTTCGATCCACATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTCCCTCCTCTAAATAATAATTCCACGCAATCCGGGGCTTTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGt                                                                                             <  1:1790481/150‑1 (MQ=255)
                                                       gTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATTCCACTCTGAACCTGAGTCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGt                                                                                             <  1:1530532/150‑1 (MQ=255)
                                                         tGGCTGCGATCCAAATCGCCGCGCGGCAATGTTCTGGAAAAAGGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTc                                                                                           <  2:188441/150‑1 (MQ=255)
                                                          ggCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATATTAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCa                                                                                          >  1:1500943/1‑150 (MQ=255)
                                                           gCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAg                                                                                         <  2:1286721/150‑1 (MQ=255)
                                                           gCTTCGATAGAAATCGCCGAGCGGAAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATGCGGGGCATTGTTGAGCGCTATTTTACGTGGTTTTTCGCGGCGGCGGTTTGTTCAg                                                                                         <  2:1318484/150‑1 (MQ=255)
                                                            cTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGCGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTGCAgc                                                                                        >  2:115403/1‑149 (MQ=255)
                                                             ttCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGAc                                                                                       <  2:1917654/150‑1 (MQ=255)
                                                                gATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGACCCTGAGACATTGTTCATCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa                                                                                    >  2:32685/1‑150 (MQ=255)
                                                                gATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa                                                                                    >  2:275888/1‑150 (MQ=255)
                                                                gATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa                                                                                    >  1:211920/1‑150 (MQ=255)
                                                                gATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCa                                                                                    >  1:1278146/1‑150 (MQ=255)
                                                                  tCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCaaa                                                                                  >  2:898659/1‑150 (MQ=255)
                                                                  tCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCaaa                                                                                  <  1:467769/150‑1 (MQ=255)
                                                                   ccAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAt                                                                                 >  1:454601/1‑150 (MQ=255)
                                                                   ccAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAGTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAt                                                                                 >  2:128767/1‑150 (MQ=255)
                                                                    cAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAtt                                                                                >  1:1128885/1‑150 (MQ=255)
                                                                    cAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAAtt                                                                                >  2:811448/1‑150 (MQ=255)
                                                                      aaTCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTaa                                                                              >  2:741846/1‑150 (MQ=255)
                                                                       aTCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGCACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAc                                                                             >  1:592422/1‑150 (MQ=255)
                                                                        tCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAcg                                                                            <  2:877640/150‑1 (MQ=255)
                                                                          gccgcGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAcgcc                                                                          <  2:1133139/150‑1 (MQ=255)
                                                                           ccgcGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAAcgccg                                                                         <  1:1909409/150‑1 (MQ=255)
                                                                               gcgGCAATGTTCTGGCAAAATGCCACTCTGACCCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCTTTTTTGAGCGCTATTTTACTTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAg                                                                     >  2:1324011/1‑150 (MQ=255)
                                                                                 ggCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGAt                                                                   <  2:1842084/150‑1 (MQ=255)
                                                                                  gCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATa                                                                  >  1:1421075/1‑150 (MQ=255)
                                                                                  gCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCAGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATa                                                                  <  1:567197/150‑1 (MQ=255)
                                                                                  gCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATATTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCAAGATa                                                                  >  1:241626/1‑150 (MQ=255)
                                                                                       gTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTACCGCCGCCAGATAAtttt                                                             >  2:252958/1‑150 (MQ=255)
                                                                                          gtGGCAAAATGCCACTCTGAACCTTAGACATTGTTACTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACt                                                          <  2:1936089/149‑1 (MQ=255)
                                                                                          cTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACt                                                          <  1:1149650/150‑1 (MQ=255)
                                                                                           tGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTg                                                         <  1:1633849/150‑1 (MQ=255)
                                                                                            ggCAAAATGCCACTCTTAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGCGCGCTATTTTACGTGTTTTTTCTCGGCGGCGGTTTGTTCAGACGCAAATTAACGCAGCAAGATAATTTTACTGa                                                        >  1:147244/1‑150 (MQ=255)
                                                                                              cAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACt                                                      >  1:1724538/1‑150 (MQ=255)
                                                                                              cAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACt                                                      >  1:1759207/1‑150 (MQ=255)
                                                                                              cAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACt                                                      >  2:1403707/1‑150 (MQ=255)
                                                                                                        ctctGACCCTGAGCCATTGTTCCTCCTGAATATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTATACTGACTATCCAGttat                                            >  2:53972/1‑148 (MQ=255)
                                                                                                        ctctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGtttt                                            >  2:1203046/1‑150 (MQ=255)
                                                                                                         tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGttttt                                           <  1:1621891/150‑1 (MQ=255)
                                                                                                         tctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGttttt                                           <  2:1444071/150‑1 (MQ=255)
                                                                                                          ctGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTc                                          <  1:809800/150‑1 (MQ=255)
                                                                                                            gAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAg                                        <  1:1056337/150‑1 (MQ=255)
                                                                                                            gAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACATGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAg                                        <  2:1494904/150‑1 (MQ=255)
                                                                                                                 tGAGACATTGTTCCTCCTGAAAATTATAATGCCACGCAATCCGTGGCATTGTTGAGCGGTTTTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTAAGTCGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTAt                                   >  2:1350295/1‑150 (MQ=255)
                                                                                                                 tGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTAt                                   <  2:1184774/150‑1 (MQ=255)
                                                                                                                  gataCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATc                                  >  1:1377293/4‑150 (MQ=255)
                                                                                                                     aCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTc                               <  1:1889244/150‑1 (MQ=255)
                                                                                                                         tGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGaa                           <  2:1562886/150‑1 (MQ=255)
                                                                                                                         tGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGaa                           <  1:660779/150‑1 (MQ=255)
                                                                                                                               tcctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTCACGCCGCCAGATTATTTTACTGACTTTCCAGTTTTTCATGTTATCTTCCGAAGGCAtt                     >  1:338663/1‑150 (MQ=255)
                                                                                                                               tcctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCAtt                     >  1:152415/1‑150 (MQ=255)
                                                                                                                                cctGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTTCGAAGGCATTa                    >  2:1982947/1‑150 (MQ=255)
                                                                                                                                cctGAAAATAATAATGCCACGCAATCCGTAGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTa                    >  2:680203/1‑150 (MQ=255)
                                                                                                                                  tGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACTTGTTTTTTCGCGGAGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGTGTATCGTCCGAAGGCATTAAc                  <  2:790611/150‑1 (MQ=255)
                                                                                                                                  tGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAc                  <  1:132131/150‑1 (MQ=255)
                                                                                                                                  tGAAAATAATAATGCCACGCAATCCGTGGAATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAc                  <  2:1254934/150‑1 (MQ=255)
                                                                                                                                   gAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAcc                 >  2:1690486/1‑150 (MQ=255)
                                                                                                                                   gAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAAcc                 >  2:1545539/1‑150 (MQ=255)
                                                                                                                                     aaaTAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCt               >  2:1278073/1‑150 (MQ=255)
                                                                                                                                     aaaTAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGAGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGTTATCGTCAGAAGGCATTAACCCt               >  2:377032/1‑150 (MQ=255)
                                                                                                                                        taataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCa            <  1:1322406/150‑1 (MQ=255)
                                                                                                                                        taataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCa            <  2:15961/150‑1 (MQ=255)
                                                                                                                                        caacaatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCa            >  1:773899/5‑150 (MQ=255)
                                                                                                                                          ataatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAgg          >  2:177950/1‑150 (MQ=255)
                                                                                                                                            aatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGAc        >  1:961489/1‑150 (MQ=255)
                                                                                                                                            aatGCCACGGATTGCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGAc        >  2:423108/1‑150 (MQ=255)
                                                                                                                                            aatGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGAc        <  2:316898/150‑1 (MQ=255)
                                                                                                                                               gCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCAc     >  2:1945079/1‑150 (MQ=255)
                                                                                                                                                  aCGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACGcc  <  2:198905/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTGTTTTTTCGCGGCGGCGGTTTGTTCAGACGCAAATTAACGCCGCCAGATAATTTTACTGACTTTCCAGTTTTTCAGGGTATCGTCCGAAGGCATTAACCCTTCAGGACCACGCC  >  NC_000913/2550425‑2550720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: