Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,566,490 C→T 5.7% G132D (GGT→GAT)  eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,4900CT 5.7% 382.7 / 7.7 122G132D (GGT→GAT) eutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base C (60/55);  new base T (4/3);  total (64/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

Only 100 of 130 total aligned reads displayed.

CCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGC  >  NC_000913/2566341‑2566639
                                                                                                                                                     |                                                                                                                                                     
ccAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGAc                                                                                                                                                       <  2:1053128/150‑1 (MQ=255)
ccAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGAc                                                                                                                                                       >  2:1029243/1‑150 (MQ=255)
 cagcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTGGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACCGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGAcc                                                                                                                                                      >  1:1245694/1‑150 (MQ=255)
  cgcagGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCg                                                                                                                                                     <  1:1805761/149‑1 (MQ=255)
        cGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATc                                                                                                                                               <  2:766332/150‑1 (MQ=255)
        cGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATc                                                                                                                                               >  2:703855/1‑150 (MQ=255)
          accaccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCAc                                                                                                                                             <  1:1066623/150‑1 (MQ=255)
          accaccGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCAc                                                                                                                                             <  1:1928423/150‑1 (MQ=255)
             accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGa                                                                                                                                          >  2:1814748/1‑150 (MQ=255)
             accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGAGACCGGCGATCACGGa                                                                                                                                          >  2:710612/1‑150 (MQ=255)
             accGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGGTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACggg                                                                                                                                          >  2:1873143/1‑149 (MQ=255)
                gACGGTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTAGCGGTGCCACCGCCGATGGCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTc                                                                                                                                       <  2:947616/150‑1 (MQ=255)
                  ggTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGATCGCCGGTCACGGagccgg                                                                                                                                     <  2:1698782/149‑6 (MQ=255)
                  cGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGa                                                                                                                                     >  1:885636/1‑150 (MQ=255)
                   gTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAg                                                                                                                                    <  1:1130889/150‑1 (MQ=255)
                      gAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTg                                                                                                                                 >  2:527098/1‑150 (MQ=255)
                      gAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTg                                                                                                                                 <  1:1110300/150‑1 (MQ=255)
                       tggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGGTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGc                                                                                                                                <  2:287654/149‑1 (MQ=255)
                        ggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCg                                                                                                                               >  2:1704607/1‑150 (MQ=255)
                        ggcaggcaGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCg                                                                                                                               >  1:791219/1‑150 (MQ=255)
                             gcagTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCg                                                                                                                          >  1:1608803/1‑150 (MQ=255)
                                    cGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTgg                                                                                                                   <  1:433975/150‑1 (MQ=255)
                                     gCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGCGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAATGGGGGTGTGCCCCGGCTCCGTGCCCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGc                                                                                                                  <  1:1973199/150‑1 (MQ=255)
                                      ccgATTTTTCCGGCATCGAACAGGGCGTAGTGCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCa                                                                                                                 <  1:1842511/148‑1 (MQ=255)
                                               ccGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGGTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACaaaa                                                                                                        >  1:1216217/1‑150 (MQ=255)
                                                cGGCCTCGAACAGGGCGTAGGTCGCGGTGCCACCGCCGAGGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGGGCGGCCCGGCGCTGGCAACGACAAAAt                                                                                                       <  1:818153/150‑1 (MQ=255)
                                                   cATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGTCGATAACGGATTCGAGGTGCTGCCCGGCGCTGGCAACGACAAAATCGc                                                                                                    >  2:1211179/1‑150 (MQ=255)
                                                   cATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGc                                                                                                    >  2:2015060/1‑150 (MQ=255)
                                                   cATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGc                                                                                                    <  2:460766/150‑1 (MQ=255)
                                                   cATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGc                                                                                                    >  2:483700/1‑150 (MQ=255)
                                                    aTCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGcc                                                                                                   <  1:235918/150‑1 (MQ=255)
                                                            gggCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACt                                                                                           >  1:225467/1‑150 (MQ=255)
                                                              gCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACGCGGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCTTGACCTGCGAGCACGGATGCGAGGTGCGGCCCGGCGCTGGCGCCGACAAATTCGGCCcgcgcatga                                                                                         >  1:1925561/1‑145 (MQ=255)
                                                              gCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGAGTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTga                                                                                         >  1:281658/1‑150 (MQ=255)
                                                              gCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGGACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGAATCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGAcggc                                                                                         >  2:378261/1‑147 (MQ=255)
                                                              gCGTAGTTCGCGGTGCCACCGCCGAAGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgt                                                                                         >  2:1507937/1‑149 (MQ=255)
                                                               cggaGTTCGCGGGGCCAGCGCCGATGTCGATCTTCAGTACCCGACACAGCCGTTGTGCAGAATGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGTATTCGAGGTGCGGCCCGGCGCGGGCAACGACAAAATCGCCCAGCGACTgag                                                                                        <  1:25467/147‑1 (MQ=255)
                                                                 tAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgagag                                                                                      <  2:690393/150‑1 (MQ=255)
                                                                 tAGTTCGCGGTGCCACCGCCGATGGCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTgagag                                                                                      <  1:1522940/150‑1 (MQ=255)
                                                                   gTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGTTTCGAGGTGCGGCCCGGCGCTGGCAACGACACAATCGCCCTGCGACTGagagag                                                                                    >  1:305409/1‑150 (MQ=255)
                                                                   gTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGagagag                                                                                    >  2:833785/1‑150 (MQ=255)
                                                                     tCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGGTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAgcg                                                                                  <  2:782707/150‑1 (MQ=255)
                                                                       gcgGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGCGAGAGCGcc                                                                                >  2:1779822/1‑150 (MQ=255)
                                                                       gcgGTGCCACCGCCGATGGCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGcc                                                                                <  2:1577032/150‑1 (MQ=255)
                                                                        cgGTGCCACCGCCGATGGCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCa                                                                               <  1:1750981/150‑1 (MQ=255)
                                                                         ggTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCAt                                                                              <  2:722772/150‑1 (MQ=255)
                                                                          gTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATc                                                                             <  1:1848300/150‑1 (MQ=255)
                                                                            gCCACCGCCGATGTCGATATTAAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCAc                                                                           <  1:593031/150‑1 (MQ=255)
                                                                              cACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCAccg                                                                         <  1:483406/150‑1 (MQ=255)
                                                                                    cgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAATGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGc                                                                   <  2:1265463/150‑1 (MQ=255)
                                                                                    cgATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGc                                                                   <  2:1057666/150‑1 (MQ=255)
                                                                                     gATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCg                                                                  <  1:774907/150‑1 (MQ=255)
                                                                                       tGTCGATATTCAGTACCCGACACAGCCGTTGGTCAGAAAGGGTGTGTGCCCCGGCTCCGTGACCGTCGATCACGGATTCGAGGTTCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAg                                                                <  2:131437/150‑1 (MQ=255)
                                                                                        gTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCCCCGCCGGGCGAGc                                                               >  2:864230/1‑150 (MQ=255)
                                                                                          cGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCAt                                                             >  1:17876/1‑150 (MQ=255)
                                                                                             tatTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCACTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGc                                                          >  2:1188669/1‑150 (MQ=255)
                                                                                                tccgTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCggg                                                       <  1:1383734/147‑1 (MQ=255)
                                                                                                tCAGTACCCGCCCCAGCCGGTGTTCAGCAAGGGTCTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCggg                                                       <  1:1466331/150‑1 (MQ=255)
                                                                                                tCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCggg                                                       >  2:1383029/1‑150 (MQ=255)
                                                                                                tCAGTACCCGACACAGCCGGTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCggg                                                       <  2:1326447/150‑1 (MQ=255)
                                                                                                 cAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGt                                                      >  1:1182812/1‑150 (MQ=255)
                                                                                                   gTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGttt                                                    <  2:407086/150‑1 (MQ=255)
                                                                                                   gTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGttt                                                    >  1:1215603/1‑150 (MQ=255)
                                                                                                      cccGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTcg                                                 >  2:1586307/1‑150 (MQ=255)
                                                                                                       ccGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTcgc                                                <  2:749486/150‑1 (MQ=255)
                                                                                                           cacaGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCTCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCtt                                            >  2:675633/1‑150 (MQ=255)
                                                                                                             caGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTc                                          >  1:631025/1‑150 (MQ=255)
                                                                                                             caGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTc                                          <  2:891336/150‑1 (MQ=255)
                                                                                                             caGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTc                                          <  1:1029243/150‑1 (MQ=255)
                                                                                                               gCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCAc                                        >  2:1467581/1‑150 (MQ=255)
                                                                                                                 cGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCg                                      >  2:761252/1‑150 (MQ=255)
                                                                                                                     gttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtga                                  >  2:1611190/1‑150 (MQ=255)
                                                                                                                      ttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCCGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgat                                 >  2:1409993/1‑150 (MQ=255)
                                                                                                                      ttCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgat                                 <  2:6012/150‑1 (MQ=255)
                                                                                                                       tCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGCCTGAGAGAGCGCCATCACCGCCGGTCGAGCATTGCGGGGTTTCGAGCTTTCACCGgtggtg                                >  2:1266305/1‑150 (MQ=255)
                                                                                                                       tCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatg                                >  1:1835609/1‑150 (MQ=255)
                                                                                                                       tCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatg                                >  1:34349/1‑150 (MQ=255)
                                                                                                                        cAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGtgatga                               <  1:483700/150‑1 (MQ=255)
                                                                                                                        cAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGAGCTTTCACCGGtgatga                               >  2:1207181/1‑150 (MQ=255)
                                                                                                                         aGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCATGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATAGCGGGTTTTAGCGCTTTCACCGGtgatgat                              >  1:1226613/1‑150 (MQ=255)
                                                                                                                             aGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCCACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCCCCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGaggatgatg                          >  2:1834928/1‑150 (MQ=255)
                                                                                                                             aGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGatgatgatg                          >  1:1161125/1‑150 (MQ=255)
                                                                                                                               ggTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGc                        <  2:216988/150‑1 (MQ=255)
                                                                                                                                gTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCa                       <  2:332814/150‑1 (MQ=255)
                                                                                                                                 tttGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCAc                      >  1:672585/1‑150 (MQ=255)
                                                                                                                                  gtgtcCCCCGGCCCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCAcc                     <  2:179981/149‑1 (MQ=255)
                                                                                                                                     tgCCCCGGCTCCGGGACCGGCGAGCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCTCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGa                  <  2:1708741/150‑1 (MQ=255)
                                                                                                                                      gCCCCGGCTCCGTGACCGGCGCTCACGGAGTCGAGGTGCGGCCCGGCGCGGGCCCCGCCCAAATCGCCCAGCGACTGAGCGAGCGCCATCACCGCCGGGCGAGCATTGCGGGGTGTCGCGCGTTCACCGGTGATGATGATGGCGCCAgtc                 >  2:130429/1‑148 (MQ=255)
                                                                                                                                       cctcgaatCCGTGATCGCCGGTCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAAt                >  2:1494709/8‑150 (MQ=255)
                                                                                                                                       ccccGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTTCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAAt                >  2:1422633/1‑150 (MQ=255)
                                                                                                                                             cTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGc          <  2:432489/150‑1 (MQ=255)
                                                                                                                                             atCCGTGATCGCCGGTCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGTGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGc          >  1:1591665/2‑150 (MQ=255)
                                                                                                                                               ccGTGATCGCCGGTCACGGATACCAGCTGCGGCACGGCGCTGGCAACGACAAAATCGCCAAGCGAAAGAGAGAGAGCCATAAACGCCGATAGAACATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGCAGCAACGCtt        >  2:1387554/1‑150 (MQ=255)
                                                                                                                                               ccGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCtt        >  1:143686/1‑150 (MQ=255)
                                                                                                                                               ccGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCtt        >  1:30700/1‑150 (MQ=255)
                                                                                                                                                 gTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGCGCATTGCGGGTTTTCGCGCTTTCACCGGTGAGGATGATGGCACCAGAATCAACGCTTTc      >  2:1519700/1‑150 (MQ=255)
                                                                                                                                                     ccGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGc  <  2:1591692/150‑1 (MQ=255)
                                                                                                                                                     ccGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGc  >  2:730132/1‑150 (MQ=255)
                                                                                                                                                     ccGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCAATCACCGACGGGCGAGCATTGCGGGTTTTCGAGATTTCACCGTTGATGAAGATGGCACCAGAATCAACGCTTTCCGGc  >  1:1003142/1‑150 (MQ=255)
                                                                                                                                                     ccGGCGAGCACGGATGCGAGGTGCGGCCCGGCGCTGGCAACGTCAAATTCGCCCAGGTACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGc  <  1:1763261/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCGGTGCCACCGCCGATGTCGATATTCAGTACCCGACACAGCCGTTGTTCAGAAAGGGTTTGTGCCCCGGCTCCGTGACCGGCGATCACGGATTCGAGGTGCGGCCCGGCGCTGGCAACGACAAAATCGCCCAGCGACTGAGAGAGCGCCATCACCGCCGGGCGAGCATTGCGGGTTTTCGCGCTTTCACCGGTGATGATGATGGCACCAGAATCAACGCTTTCCGGC  >  NC_000913/2566341‑2566639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑