Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 254,223 A→T 5.4% intergenic (+21/+36) prfH → / ← pepD pseudogene, RF‑1 domain family; probable peptide chain release factor/aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913254,2230AT 5.4% 457.1 / 9.0 155intergenic (+21/+36)prfH/pepDpseudogene, RF‑1 domain family; probable peptide chain release factor/aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (75/69);  new base T (6/3);  total (82/73)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

Only 100 of 156 total aligned reads displayed.

GGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGA  >  NC_000913/254075‑254371
                                                                                                                                                    |                                                                                                                                                    
ggAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAg                                                                                                                                                     >  1:1405467/1‑150 (MQ=255)
 gAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAgg                                                                                                                                                    <  1:780888/150‑1 (MQ=255)
   aGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCg                                                                                                                                                  >  1:627793/1‑150 (MQ=255)
    gCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACTTTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGa                                                                                                                                                 <  1:343792/150‑1 (MQ=255)
    gCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGa                                                                                                                                                 >  1:723656/1‑150 (MQ=255)
    gCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGa                                                                                                                                                 <  1:226714/150‑1 (MQ=255)
    gCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGa                                                                                                                                                 <  1:1329506/150‑1 (MQ=255)
     cTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGAt                                                                                                                                                <  2:1405834/150‑1 (MQ=255)
      tGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGAtt                                                                                                                                               >  2:1527865/1‑150 (MQ=255)
       ggAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGTACATTTACAGGGATGGCTTTTATCGAAGGAAAATGAAGGATGAAACTGCCGGAAGGAGATTa                                                                                                                                              >  2:459670/1‑150 (MQ=255)
       ggAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTa                                                                                                                                              >  2:49864/1‑150 (MQ=255)
          gcaacagcaacaGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAAc                                                                                                                                           >  2:1630629/1‑150 (MQ=255)
           caacagcaacagGAAAATAGCGCGGCGCTGAACTCGCAGCGGCGAATGTTCCATCCCCAGATTGAAGGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATGGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACg                                                                                                                                          <  2:774186/150‑1 (MQ=255)
           caacagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACg                                                                                                                                          <  2:473296/150‑1 (MQ=255)
            aacagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGCTTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGc                                                                                                                                         <  2:1241659/150‑1 (MQ=255)
              cagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCCCCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGACa                                                                                                                                       >  1:987033/1‑150 (MQ=255)
              cagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCa                                                                                                                                       >  2:1658663/1‑150 (MQ=255)
              cagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCa                                                                                                                                       >  2:1536860/1‑150 (MQ=255)
              cagcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCa                                                                                                                                       >  1:269308/1‑150 (MQ=255)
               agcaacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCACCCCGCGACGAACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCAt                                                                                                                                      >  2:1491573/1‑150 (MQ=255)
                 caacagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATcc                                                                                                                                    <  1:1140168/150‑1 (MQ=255)
                   acagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCgg                                                                                                                                  >  2:60818/1‑150 (MQ=255)
                   acagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCgg                                                                                                                                  >  2:633847/1‑150 (MQ=255)
                    cagGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGGATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgc                                                                                                                                 <  2:1461863/150‑1 (MQ=255)
                     agGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgca                                                                                                                                <  2:225140/150‑1 (MQ=255)
                     agGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgca                                                                                                                                <  1:1060415/150‑1 (MQ=255)
                     agGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgca                                                                                                                                <  2:1966118/150‑1 (MQ=255)
                      ggAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGCATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGgcac                                                                                                                               >  2:926742/1‑149 (MQ=255)
                      ggAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCAGCGACGGACATTTTCACGGATGGCTTTTAAAGAAGGATAATGAAGGATGAAACTGCCTGAAGGCGAATAAACGCCATCCCgcac                                                                                                                               >  2:344704/1‑149 (MQ=255)
                             aGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGTTTAATCGCCTTCCGgcagtttcatc                                                                                                                        <  1:596785/150‑8 (MQ=39)
                               cgcgGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCACCCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAACCTGCCGGAAGGCGATTAAACGCAATCAGGCAGcaaatcaaa                                                                                                                      >  1:345857/1‑150 (MQ=255)
                                  ggcgCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataa                                                                                                                   <  2:710314/150‑1 (MQ=255)
                                   gcgcTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAaataat                                                                                                                  >  2:344341/1‑150 (MQ=255)
                                      cTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGATGGACATTTACAGGGATGGCTTTTATCGAAGGATATGGATGGATGAAACTGCCGGACGGCGATTAAACGCCATCCGGCAGCAAATCAAATAAGTag                                                                                                               >  1:329717/1‑149 (MQ=255)
                                      cTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTAc                                                                                                               <  2:436926/150‑1 (MQ=255)
                                       tGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACt                                                                                                              >  2:857859/1‑150 (MQ=255)
                                       tGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACt                                                                                                              >  1:239988/1‑150 (MQ=255)
                                         aaaTCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTc                                                                                                            >  2:1303493/1‑150 (MQ=255)
                                                     cgAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttct                                                                                                <  1:930703/150‑1 (MQ=255)
                                                        aTGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAAtttctttc                                                                                             <  2:1734521/150‑1 (MQ=255)
                                                            tCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagca                                                                                         >  1:442682/1‑150 (MQ=255)
                                                             ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGATGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                                                                        >  1:112155/1‑150 (MQ=255)
                                                             ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                                                                        <  1:1376158/150‑1 (MQ=255)
                                                             ccATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTcagcag                                                                                        >  1:1702968/1‑150 (MQ=255)
                                                              cATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGt                                                                                       >  2:1507227/1‑150 (MQ=255)
                                                               aTCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGtt                                                                                      >  2:1728355/1‑150 (MQ=255)
                                                               aTCACCAGATTGAACGTGGCAACCAGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGtt                                                                                      <  1:1202170/150‑1 (MQ=255)
                                                                tCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtc                                                                                     <  2:1846631/150‑1 (MQ=255)
                                                                   ccAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTtcagt                                                                                  >  1:2004271/1‑150 (MQ=255)
                                                                       aTTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagc                                                                              >  2:411142/1‑150 (MQ=255)
                                                                       aTTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagc                                                                              <  2:269308/150‑1 (MQ=255)
                                                                       aTTGAACGGGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagc                                                                              <  2:1441444/150‑1 (MQ=255)
                                                                        ttGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTcagca                                                                             <  2:687767/150‑1 (MQ=255)
                                                                           aaCGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAgtg                                                                          <  1:1773392/150‑1 (MQ=255)
                                                                             cGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCGGTGTc                                                                        >  1:1997503/1‑150 (MQ=255)
                                                                              gTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTcc                                                                       <  1:1176823/150‑1 (MQ=255)
                                                                               tGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCa                                                                      <  2:1294710/150‑1 (MQ=255)
                                                                                ggCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAg                                                                     >  2:744653/1‑150 (MQ=255)
                                                                                     cccGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATg                                                                <  2:1405467/150‑1 (MQ=255)
                                                                                     cccGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATg                                                                >  1:1996876/1‑150 (MQ=255)
                                                                                           aCGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGCATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTAc                                                          >  1:1049668/1‑150 (MQ=255)
                                                                                            cGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACg                                                         <  2:850202/150‑1 (MQ=255)
                                                                                            cGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACg                                                         >  1:748479/1‑150 (MQ=255)
                                                                                             ggACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc                                                        >  2:1277333/1‑150 (MQ=255)
                                                                                             ggACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc                                                        <  2:252865/150‑1 (MQ=255)
                                                                                             ggACATTTACAGGGATGGCTGTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCTGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGc                                                        <  2:1688034/150‑1 (MQ=255)
                                                                                              gACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCt                                                       <  2:1622652/150‑1 (MQ=255)
                                                                                               aCATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCtt                                                      <  1:1658663/150‑1 (MQ=255)
                                                                                                cATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCttt                                                     <  1:1630629/150‑1 (MQ=255)
                                                                                                  tttACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCACTCAGCAGTGTCCAGTAATGACCTACGCTTTCg                                                   <  2:1035780/150‑1 (MQ=255)
                                                                                                      cAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCCGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAtgg                                               >  2:1332467/1‑149 (MQ=255)
                                                                                                        gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTATTTCAGCAGTTCAGTCAGCAGTGTCCAGGAATGACCTACGCTTTCGATGTGa                                             >  2:554535/1‑150 (MQ=255)
                                                                                                        gggATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAAATACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGa                                             >  2:1094599/1‑150 (MQ=255)
                                                                                                            tGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACtt                                         >  1:980246/1‑150 (MQ=255)
                                                                                                              gCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGc                                       >  2:1261297/1‑150 (MQ=255)
                                                                                                                      cGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCgg                               <  2:1589009/150‑1 (MQ=255)
                                                                                                                       gAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGGTCAGTCCGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGga                              >  1:1279599/1‑150 (MQ=255)
                                                                                                                          ggATAATGAAGGATGAAACTTCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCACTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGagag                           >  1:1500372/1‑150 (MQ=255)
                                                                                                                             tAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAgtgt                        <  1:447607/150‑1 (MQ=255)
                                                                                                                              aaTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGtgtg                       <  1:411142/150‑1 (MQ=255)
                                                                                                                               aTGAAGGATGCAACTGCCGGACGGCGAGTAAACGCCATCCGGCAGCAAATCAAATCCTTCCGTCGCCGGCATTGCTTTCAGGAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGCTGTGAACTTGCTCATCCGGAGAGGGTgg                      <  1:344707/150‑1 (MQ=255)
                                                                                                                               aTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTgg                      <  1:241762/150‑1 (MQ=255)
                                                                                                                               aTGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTAAGCAGTTCAGACAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTATACCGGAGAGTGTgg                      >  2:352545/1‑150 (MQ=255)
                                                                                                                                tGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGa                     <  1:633847/150‑1 (MQ=255)
                                                                                                                                 gAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGAGGTGAACTTGATCATCCGGAGAGTTTGGAc                    >  2:1051402/1‑150 (MQ=255)
                                                                                                                                     gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGt                <  2:1622297/150‑1 (MQ=255)
                                                                                                                                     gATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGt                <  1:690000/150‑1 (MQ=255)
                                                                                                                                         aaaCTGCCGCAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATAACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCTGAGAGTGTGGACCGCTGATa            <  2:1002813/150‑1 (MQ=255)
                                                                                                                                          aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAg           <  2:1777923/150‑1 (MQ=255)
                                                                                                                                          aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAg           <  2:627793/150‑1 (MQ=255)
                                                                                                                                          aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAg           >  1:940423/1‑150 (MQ=255)
                                                                                                                                             tGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTg        >  1:1972546/1‑150 (MQ=255)
                                                                                                                                              gCCGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGTTGATAGTTgg       >  2:1052340/1‑150 (MQ=255)
                                                                                                                                              gCCGGAAGGCGATTAATCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTgg       <  2:1037302/150‑1 (MQ=255)
                                                                                                                                              gCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTgg       >  1:392952/1‑150 (MQ=255)
                                                                                                                                               ccGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATTATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGGCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGATCTTGCTAATCCGGAGAGTGTGGACCGGTGATAGTTGGc      >  1:1777042/1‑150 (MQ=255)
                                                                                                                                               ccGGATGGCGTTTAATCGCCTTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGc      >  2:179664/1‑150 (MQ=255)
                                                                                                                                               ccGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTAAGTCAGCAGTGTCCAGTAATGA‑CTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGcc     <  1:1689356/150‑1 (MQ=255)
                                                                                                                                                cGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGcc     >  2:762269/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGATTAAACGCCATCCGGCAGCAAATCAACTAATTACTTCGTCGGAATTTCTTTCAGCAGGTCAGGCAGCATTGTCCCGTACTGACCTACGCTTTCGCTGTGAACTTGCTCATCCGGAGAGTGTGGCCCGGTGATAGTTGGCCCGa  <  2:345857/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGAAGCTGGAGCAACAGCAACAGGAAAATAGCGCGGCGCTGAAATCGCAGCGGCGAATGTTCCATCACCAGATTGAACGTGGCAACCCGCGACGGACATTTACAGGGATGGCTTTTATCGAAGGATAATGAAGGATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGA  >  NC_000913/254075‑254371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑