Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,790 G→T 5.4% intergenic (+22/+26) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7900GT 5.4% 440.1 / 8.4 145intergenic (+22/+26)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base G (74/62);  new base T (5/4);  total (79/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

ACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACA  >  NC_000913/2673641‑2673938
                                                                                                                                                     |                                                                                                                                                    
aCTGGGATATGACCGGCGATGTGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTg                                                                                                                                                      <  2:1279583/150‑1 (MQ=255)
 cTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                                                                                     >  1:1174639/1‑150 (MQ=255)
      atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                                                                <  1:554204/150‑1 (MQ=255)
         tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             >  1:1113525/1‑150 (MQ=255)
         tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             >  2:1876550/1‑150 (MQ=255)
         tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCAGGGAGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             >  2:574720/1‑150 (MQ=255)
         ggACCGGCGATGCGGTAGGGCCAAATATGATTAATTTCTGGTAGAATGTCAGTATCGCTGGCTCGTTCTTTCTATTCGCAATTACGGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                             <  1:119208/149‑1 (MQ=255)
          gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                            >  1:1313989/1‑150 (MQ=255)
           aCCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                           <  2:1841599/150‑1 (MQ=255)
           aCCGGCGATGCGGGTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                           <  2:162718/150‑1 (MQ=255)
             cGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                         >  2:1379638/1‑150 (MQ=255)
              ggCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                        >  1:2013822/1‑150 (MQ=255)
                 gaggCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTTGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                     <  2:273355/147‑1 (MQ=255)
                 gATGCGGTTTGGCCAAATATGATTAATTTCAGGAAGAATGTCAGTATCGCTGGCGCGATCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                     <  1:1049545/150‑1 (MQ=255)
                 gATGCGGTTGGGCCATATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCTGCAt                                                                                                                                     >  2:708680/1‑150 (MQ=255)
                   tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                                   <  1:836529/150‑1 (MQ=255)
                   tGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAttt                                                                                                                                   <  2:1713520/150‑1 (MQ=255)
                   tGCGGCTGGGCCAAATATGATTAATTTCTGGAAGACTGTCAGTATCGCTGGCGCGTTCTAGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAtct                                                                                                                                   <  1:1808920/150‑3 (MQ=38)
                     cGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                                                                                 <  1:933186/150‑1 (MQ=255)
                       gTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaata                                                                                                                               >  2:154215/1‑150 (MQ=255)
                        gtGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGGTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaataa                                                                                                                              <  2:1356280/149‑1 (MQ=255)
                         tGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATaaa                                                                                                                             <  1:1021436/150‑1 (MQ=255)
                         ggggACAAATATGATTAATTTCTGGAAGAATTTCAGTCTCGCGGGCTCGTTCTTGATATTGGCAATTCCCGGGCAGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCAGGATGCGGCTTTAACGACTTATCCGGCATTTAATaaa                                                                                                                             <  1:785174/149‑1 (MQ=255)
                            gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTa                                                                                                                          <  2:55558/150‑1 (MQ=255)
                              cAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACa                                                                                                                        <  2:538551/150‑1 (MQ=255)
                               aaaTATGCTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAg                                                                                                                       <  2:1946546/150‑1 (MQ=255)
                                 atatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGcc                                                                                                                     >  2:247014/1‑150 (MQ=255)
                                  tatGATTAATTTCTGGAAGAATGTCAGTATCGCAGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCa                                                                                                                    >  1:179351/1‑150 (MQ=255)
                                    tGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                                  <  1:580358/150‑1 (MQ=255)
                                      aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActca                                                                                                                >  2:1956880/1‑149 (MQ=255)
                                           tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCg                                                                                                           >  1:39129/1‑150 (MQ=255)
                                            ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGc                                                                                                          <  1:663090/150‑1 (MQ=255)
                                              cTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCaa                                                                                                        >  1:1885375/1‑150 (MQ=255)
                                              cTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCaa                                                                                                        >  1:965908/1‑150 (MQ=255)
                                                   agaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACa                                                                                                   >  2:378341/1‑150 (MQ=255)
                                                   agaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACa                                                                                                   >  2:794098/1‑150 (MQ=255)
                                                      aTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtct                                                                                                >  1:1472210/1‑150 (MQ=255)
                                                        gTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCCCTCTACCCGCAACGACATctct                                                                                              >  2:242398/1‑150 (MQ=255)
                                                        gTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATctct                                                                                              >  1:1995923/1‑150 (MQ=255)
                                                        gTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCCTGTACTCCTTTTCCGGCATTTAAAAAATTAAAGCCACTCAACCCGCAACGACATctct                                                                                              >  1:1826360/1‑150 (MQ=255)
                                                         tCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTg                                                                                             <  1:804782/150‑1 (MQ=255)
                                                          cAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGa                                                                                            <  1:448891/150‑1 (MQ=255)
                                                           aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                           >  2:1927755/1‑150 (MQ=255)
                                                           aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                           >  1:614996/1‑150 (MQ=255)
                                                            gTATCGCTGCCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                                                          <  1:1300328/150‑1 (MQ=255)
                                                              aTCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgtt                                                                                        >  2:1300784/1‑150 (MQ=255)
                                                                  gtGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGttgttg                                                                                    <  2:2004783/149‑1 (MQ=255)
                                                                  cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGttgttg                                                                                    <  2:797071/150‑1 (MQ=255)
                                                                  cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGttgttg                                                                                    <  2:1118465/150‑1 (MQ=255)
                                                                       gcgGTCTTGCTATTGTCAATTACCGGGCCGGGGGCAATGTCTCTCGATCGGCGTAAGTAAAGATGCCGGATGCGGCGTGAACGCCTTATCCTGCATTTAATAAATTACGGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcc                                                                               <  1:130112/150‑1 (MQ=255)
                                                                       gcgGTCTTGCTATTGGCAATTACCGTGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCTTATGCGGCGTGAACGCCTTATCCGGCTTTTAATAAATTACAGCCACTCAACCCGCAACGACATCTCTGAAGTTGTTGATTcc                                                                               <  2:847110/150‑1 (MQ=255)
                                                                           tCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTg                                                                           <  1:511748/150‑1 (MQ=255)
                                                                            cTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTgg                                                                          >  1:795270/1‑150 (MQ=255)
                                                                              tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                        >  1:105743/1‑150 (MQ=255)
                                                                              tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                        <  1:154215/150‑1 (MQ=255)
                                                                              tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg                                                                        <  1:1404662/150‑1 (MQ=255)
                                                                                  aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                    <  1:1829448/150‑1 (MQ=255)
                                                                                  aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAGCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                    >  2:1383583/1‑150 (MQ=255)
                                                                                  aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGACTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                    >  1:1129103/1‑150 (MQ=255)
                                                                                     ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTAATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc                                                                 >  2:1234832/1‑150 (MQ=255)
                                                                                       cAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                               >  1:1685480/1‑150 (MQ=255)
                                                                                          ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATga                                                            <  1:1267219/150‑1 (MQ=255)
                                                                                          ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATga                                                            >  1:1924989/1‑150 (MQ=255)
                                                                                            aCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTATGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATgaga                                                          <  1:708680/150‑1 (MQ=255)
                                                                                            aCCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTGCAGCCACTCAACCCGCAACGCCATCTCAGAAGTTGTTGATTACCCTGGTGCTAGCGCAATgaga                                                          >  1:990455/1‑150 (MQ=255)
                                                                                             ccgggccgtgGGCAATTTCTCTAGATCGGCGTTATGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAGGACATCTCTGAAGTTGTGGATTCCCCTGGTGCTAGCGCAATGAGAt                                                         <  2:304246/150‑1 (MQ=255)
                                                                                             ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACCCCGTAGCCGGCATTTAATTAAATACAGCCACTCTACCCGCCCCGCGAGGTCTTAAGTTGTTGATTCCCCTGGTGCTAGCGCAAttacat                                                         >  1:1934362/1‑145 (MQ=255)
                                                                                                 gccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACACTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcc                                                     >  2:711840/1‑150 (MQ=255)
                                                                                                  ccgggGGCAATTTCTCTCGATCGTCGATAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATAACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAccc                                                    <  2:1491343/150‑1 (MQ=255)
                                                                                                         cAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCg                                             >  2:641448/1‑150 (MQ=255)
                                                                                                           aTTTCTCTGGCTCTGAGTTAGGAAAGCTGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTATTAAATTACCGCCACTCTCCCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCggg                                           <  2:988335/150‑1 (MQ=255)
                                                                                                            tttCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGc                                          <  1:1232399/150‑1 (MQ=255)
                                                                                                               ctctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                       <  1:1379638/150‑1 (MQ=255)
                                                                                                                tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatg                                      >  1:633373/1‑150 (MQ=255)
                                                                                                                tctcGATCGGCGTTAGGAAAGATGCAGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatg                                      <  2:104234/150‑1 (MQ=255)
                                                                                                                   cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCTCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                   >  2:1739273/1‑150 (MQ=255)
                                                                                                                   cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                   <  1:558996/150‑1 (MQ=255)
                                                                                                                   cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                   <  2:347092/150‑1 (MQ=255)
                                                                                                                    gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                                  >  2:83411/1‑150 (MQ=255)
                                                                                                                    gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                                  >  2:693304/1‑150 (MQ=255)
                                                                                                                     aTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatgat                                 <  2:1693745/150‑1 (MQ=255)
                                                                                                                            ttAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCgg                          >  1:1564870/1‑150 (MQ=255)
                                                                                                                            ttAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATAACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCgg                          <  2:954311/150‑1 (MQ=255)
                                                                                                                              aGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAGCGCTGAAGTTGTTGAGTCCCCTGGTGCTAGCGCAATGAGATCACACCCTTCCGGGCGATGATGATCATCCGgcg                        >  2:1566613/1‑150 (MQ=255)
                                                                                                                               ggAAAGATGCCGGTTGCGGCGTGAACGCCTTATCCGGGATTTAATAAATTACAGCCACGCTACCCGCAACGCCATCTCTGAAGTTGGTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                       >  1:198272/1‑150 (MQ=255)
                                                                                                                               ggAAAGATGCCGGATGCGGCGTGAACGCCTTATACGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCAGAAGTTGATGATTCCCCTGGTGCTAGCCCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcga                       >  2:1609192/1‑149 (MQ=255)
                                                                                                                                gAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCa                      <  1:247014/150‑1 (MQ=255)
                                                                                                                                   aGATTCCTGATGCGGCGTGAACGCCGTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACTACATCTCTGAAGTGGTTGATTCCCCTCGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATgg                   <  2:1450346/150‑1 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa               >  2:1598951/1‑150 (MQ=255)
                                                                                                                                        ccGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAATCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTcct              >  1:1401976/1‑148 (MQ=255)
                                                                                                                                         cGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc             <  1:258433/150‑1 (MQ=255)
                                                                                                                                         cGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATc             <  2:1751093/150‑1 (MQ=255)
                                                                                                                                           gATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCgg           >  1:502180/1‑150 (MQ=255)
                                                                                                                                           gATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCgg           <  2:1995923/150‑1 (MQ=255)
                                                                                                                                              gcggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCCAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTc        <  2:1562194/150‑1 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcag     >  1:314599/1‑150 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcag     >  1:1658916/1‑150 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcag     <  2:1491576/150‑1 (MQ=255)
                                                                                                                                                   gTGAACGCCTTATCAGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagac   <  2:1220118/150‑1 (MQ=255)
                                                                                                                                                    tGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagaca  >  2:1285762/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACA  >  NC_000913/2673641‑2673938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: