Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,770,319 C→G 6.2% P206R (CCG→CGG)  yfjR → CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,770,3190CG 6.2% 402.8 / 15.1 130P206R (CCG→CGG) yfjRCP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (58/63);  new base G (3/5);  total (62/68)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

Only 100 of 134 total aligned reads displayed.

CCGGTTACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGCCATTATCGCC  >  NC_000913/2770170‑2770468
                                                                                                                                                     |                                                                                                                                                     
ccGGTTACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAATTCCATCTGTTTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGAccc                                                                                                                                                       <  1:400859/150‑1 (MQ=255)
ccGGTTACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGAccc                                                                                                                                                       <  1:742538/150‑1 (MQ=255)
ccGGTTACTCATCCACCAGCAGACCTCGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTAGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGAccc                                                                                                                                                       <  2:819937/150‑1 (MQ=255)
 cGGTTACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCg                                                                                                                                                      <  2:1366545/150‑1 (MQ=255)
     tACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCt                                                                                                                                                  <  2:447543/150‑1 (MQ=255)
     tACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCt                                                                                                                                                  <  1:1724190/150‑1 (MQ=255)
     tACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAACGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTTCAGGAGACTTTTCTCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCt                                                                                                                                                  <  1:417836/150‑1 (MQ=255)
      aCTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCtt                                                                                                                                                 <  2:1790463/150‑1 (MQ=255)
      aCTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCtt                                                                                                                                                 <  2:1766234/150‑1 (MQ=255)
       cTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCttc                                                                                                                                                <  2:1675223/150‑1 (MQ=255)
       cTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCttc                                                                                                                                                >  2:480058/1‑150 (MQ=255)
         cATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACCCTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCATTGACAGTCTGTGTCGCCTGGTTGAAGCCCCGGTCttcat                                                                                                                                              >  1:1088217/1‑150 (MQ=255)
         cATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGCCCCGGTCttcat                                                                                                                                              >  2:1761039/1‑150 (MQ=255)
         cATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCttcat                                                                                                                                              <  1:363743/150‑1 (MQ=255)
            ccaccaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCttcattca                                                                                                                                           >  1:483390/1‑150 (MQ=255)
            ccaccaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCttcattca                                                                                                                                           >  1:345536/1‑150 (MQ=255)
            ccaccaGCAGACCTGGAATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCGGGTCttcaacca                                                                                                                                           <  1:1796569/150‑5 (MQ=255)
             caccaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAg                                                                                                                                          <  1:1710146/150‑1 (MQ=255)
              accaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCGGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAgg                                                                                                                                         <  1:1163407/150‑1 (MQ=255)
              accaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAgg                                                                                                                                         <  1:184510/150‑1 (MQ=255)
              accaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCAGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGAATGAAGACCGGGTCTTCAACCAgg                                                                                                                                         <  2:1483593/150‑1 (MQ=255)
               ccaGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGc                                                                                                                                        <  1:1059846/150‑1 (MQ=255)
               ccaGCAGAACTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGc                                                                                                                                        >  2:451460/1‑150 (MQ=255)
                  gcagACCTGGTATCTGGTGGCTGAACACGAAGGGCATTTCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGAATGAAGACCGGGTCTTCAACCAGGcgac                                                                                                                                     <  2:703841/150‑4 (MQ=255)
                  gcagACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCtt                                                                                                                                     >  2:1225086/1‑150 (MQ=255)
                  gcagACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGAATGAAGACCGGGTCTTCAACCAGGcgac                                                                                                                                     <  2:1072940/150‑4 (MQ=255)
                     gACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTAcc                                                                                                                                  <  1:207629/150‑1 (MQ=255)
                      aCCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTAccc                                                                                                                                 <  1:657591/150‑1 (MQ=255)
                      aCCTGGTATCTGGTGGCTGAACACGAAGGGCATATAGCCGTATTCACACTAGATGAAAACCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGAATGAAGACCGGGTCTTCAACCAGGcgacacag                                                                                                                                 <  2:1949104/150‑8 (MQ=38)
                       ccTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTAcccc                                                                                                                                >  1:1475500/1‑150 (MQ=255)
                        cTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGCCCCGGTCTTCATTCAGGCCTTACCCCa                                                                                                                               >  1:398965/1‑150 (MQ=255)
                          ggTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCAtt                                                                                                                             >  1:1768294/1‑150 (MQ=255)
                           gTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCAttt                                                                                                                            <  2:1449001/150‑1 (MQ=255)
                                tggtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACACCATTTTCGCt                                                                                                                       >  1:382462/1‑150 (MQ=255)
                                 ggtggCTGAACACGAAGGGCATATAGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCtt                                                                                                                      <  1:1358515/150‑1 (MQ=255)
                                  gtggCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCttt                                                                                                                     <  2:103293/150‑1 (MQ=255)
                                      cTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAACTCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAATACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTGTATcc                                                                                                                 >  1:284920/1‑150 (MQ=255)
                                      cTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACGCCATTTTCGATTTATcc                                                                                                                 >  1:196988/1‑150 (MQ=255)
                                      cTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATcc                                                                                                                 >  2:1148343/1‑150 (MQ=255)
                                         aaCACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGc                                                                                                              >  1:1236010/1‑150 (MQ=255)
                                         aaCACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGc                                                                                                              <  1:1007946/150‑1 (MQ=255)
                                           cacGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCATTACCCCATTTTCGCTTTATACAGCAt                                                                                                            >  1:1699883/1‑150 (MQ=255)
                                            acGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCAtt                                                                                                           <  2:153691/150‑1 (MQ=255)
                                             cGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTc                                                                                                          >  1:682794/1‑150 (MQ=255)
                                               aaGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCAc                                                                                                        >  1:1852687/1‑150 (MQ=255)
                                                 gggCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTg                                                                                                      >  1:619992/1‑150 (MQ=255)
                                                 gggCATATAGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTg                                                                                                      >  2:661129/1‑150 (MQ=255)
                                                  ggCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGc                                                                                                     <  1:230642/150‑1 (MQ=255)
                                                         cGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGtt                                                                                              <  1:451460/150‑1 (MQ=255)
                                                           ccGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTg                                                                                            <  2:642908/150‑1 (MQ=255)
                                                           ccGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTg                                                                                            <  1:1023378/150‑1 (MQ=255)
                                                            cGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGt                                                                                           <  1:995531/150‑1 (MQ=255)
                                                            cGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGt                                                                                           <  1:709681/150‑1 (MQ=255)
                                                               aTTCACACTTGATGAAATCCATCTGATGCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTcc                                                                                        <  1:579298/150‑1 (MQ=255)
                                                                  cacaCTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGc                                                                                     <  1:913717/150‑1 (MQ=255)
                                                                  cacaCTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGc                                                                                     <  1:29708/150‑1 (MQ=255)
                                                                  cacaCTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAACATTCACTGCTTACGTTTGTTCCGGc                                                                                     >  1:898514/1‑150 (MQ=255)
                                                                    cacTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCg                                                                                   <  1:480058/150‑1 (MQ=255)
                                                                      cTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGAc                                                                                 >  1:1989675/1‑150 (MQ=255)
                                                                      cTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTACGGCCGAc                                                                                 >  2:1735696/1‑150 (MQ=255)
                                                                         gatgaAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGc                                                                              >  1:1415730/1‑150 (MQ=255)
                                                                           tgaAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGccc                                                                            >  2:141767/1‑150 (MQ=255)
                                                                              aaTCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCAcc                                                                         >  2:554534/1‑150 (MQ=255)
                                                                                   aTCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAAt                                                                    >  2:1726956/1‑150 (MQ=255)
                                                                                    tCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATa                                                                   >  1:1274306/1‑150 (MQ=255)
                                                                                     cTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAg                                                                  <  2:1980727/150‑1 (MQ=255)
                                                                                        aTTCAGCCATTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAgcgc                                                               <  2:160739/150‑1 (MQ=255)
                                                                                             gCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCg                                                          <  1:1225086/150‑1 (MQ=255)
                                                                                              ccTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCACATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCgt                                                         >  1:205765/1‑150 (MQ=255)
                                                                                                    cAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCTGCCTAAATCCCACCGTAATAGCGCAGGCGTTGTTAt                                                   >  2:334072/1‑150 (MQ=255)
                                                                                                     aGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGATTAGCGCAGGCGTTGTTATc                                                  <  2:1520123/150‑1 (MQ=255)
                                                                                                     aGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGACGACAGCCCACCGGAATAGCGCAGGCGTTGTTATc                                                  >  2:347032/1‑150 (MQ=255)
                                                                                                      ggAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCa                                                 <  2:397495/150‑1 (MQ=255)
                                                                                                         gaCTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAAcc                                              >  1:1048288/1‑150 (MQ=255)
                                                                                                          aCTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAAccc                                             <  2:1236010/150‑1 (MQ=255)
                                                                                                           cTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCg                                            >  2:33251/1‑150 (MQ=255)
                                                                                                            ttttCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCgg                                           <  2:623746/150‑1 (MQ=255)
                                                                                                                cgccgcAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAACATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAAc                                       <  2:898514/150‑1 (MQ=255)
                                                                                                                    gcAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATgg                                   >  1:1733506/1‑150 (MQ=255)
                                                                                                                    gcAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATgg                                   >  1:1999957/1‑150 (MQ=255)
                                                                                                                    gcAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTAACCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATgg                                   <  2:217143/150‑1 (MQ=255)
                                                                                                                       aTGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTAAGGCATTACCCAATTTTAGCTTTATAAACCATTCACTGCTTACGTTTGTTACGGCCGACAGCACCCCGGAATAGCGCAGGAGTTGTTATCAACCCCTCAACATggacg                                >  2:1736853/1‑148 (MQ=255)
                                                                                                                        tGACAGTCTGTCTCGCCTGGTAGAAGACCCGGTCTTCATTCAGGCCTTACCCCAATTTCGCTTTATCCAGCATTCACTGCTTACGTTAGTTCCGGCCGACCGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATggagga                               >  2:1438816/1‑150 (MQ=255)
                                                                                                                         gACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGATGTAATCAACCCGGCAACATGgaggag                              >  2:1887953/1‑150 (MQ=255)
                                                                                                                         gACAGTCTGTGGCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGgaggag                              <  2:1880119/150‑1 (MQ=255)
                                                                                                                             gTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCa                          <  2:1000241/150‑1 (MQ=255)
                                                                                                                                  tgtCGCCTGGATGAAGACCCGGTCTTCATTCAGGACTTACACCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACTGCCGACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGTAGGAGCCCAGTGcc                     >  1:381281/1‑150 (MQ=255)
                                                                                                                                     cGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCACCATGGAGGAGCCCAGTGCCCGt                  >  2:959623/1‑150 (MQ=255)
                                                                                                                                       cctgaatGAAGACCGGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTa                >  2:1687132/7‑150 (MQ=255)
                                                                                                                                       cctgaatGAAGACCGGGTCTTCAACCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGCCAGCCCACCGGAATAGCGCAGGCGTTGTTATCTACCCGGCACCATGGAGGAGCCCAGTGCGCGttt                >  1:340434/7‑149 (MQ=255)
                                                                                                                                       ccTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTa                <  2:1993256/150‑1 (MQ=255)
                                                                                                                                        cTGGTTGAAGCCCCGGTATTCATTAAGGCCTTACCCCATTTTCGCGTTATCCAGCATTCACGGCTTACGTTTGTTCCGGAAGACATCAAACAGTAATAGCTCAGGCGGTGTGATCAACACGGCAACAGGGAGGAGCCCAGTGCCCGTTAt               >  2:1038260/1‑150 (MQ=255)
                                                                                                                                         tGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTAtt              >  2:875137/1‑150 (MQ=255)
                                                                                                                                         ggtttGAAGACAAGGTCTTCATTCAGGCCTTACCCCATTTTCGATATATCCAGCATTCAATGCATACGTTTGTTCCGGACGACAGCCCTCCGGAATAAAGCAGGCGTTGTTATCAACACGGAAACATGGAGGAGCCACGTGCCCGtttat              >  2:1334544/4‑147 (MQ=255)
                                                                                                                                           gtggAAGACCCGGGCTGCATTCAGGCCTTACCCCATTTTCGCGTTATCCAGCATTCACTGCTTCCGTTTGTGCCGGCCGCCAGCCCACCGGAATAGCGCAGGCGTTGTTAGCACCCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGc            <  1:253196/147‑1 (MQ=255)
                                                                                                                                           gTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGc            <  1:1838749/150‑1 (MQ=255)
                                                                                                                                           aatGAAGACCGGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGGGCCCAGTGCCCGTTATTgg            >  1:713178/3‑149 (MQ=255)
                                                                                                                                              gAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGCCAt         >  2:1393020/1‑150 (MQ=255)
                                                                                                                                                  aCCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTGGTTATAAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGCCATTATc     >  2:800383/1‑150 (MQ=255)
                                                                                                                                                     cGGTCTTCATTCCGGCCGGACCCCATTTTCGCTTTATCCCGCCTTCACTGCGTACGATTGTTCAGGCCGACCGCCCCCCGGAATAGCGCAGGCGTTGTACTCAACCCGGCAACATGGAGGAGCCCCGGGCCCGGTCTTGCCATTATCGcc  <  2:1024593/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGGTTACTCATCCACCAGCAGACCTGGTATCTGGTGGCTGAACACGAAGGGCATATCGCCGTATTCACACTTGATGAAATCCATCTGATTCAGCCTTTGCAGGAGACTTTTCGCCGCAATGACAGTCTGTGTCGCCTGGTTGAAGACCCGGTCTTCATTCAGGCCTTACCCCATTTTCGCTTTATCCAGCATTCACTGCTTACGTTTGTTCCGGCCGACAGCCCACCGGAATAGCGCAGGCGTTGTTATCAACCCGGCAACATGGAGGAGCCCAGTGCCCGTTATTGCCATTATCGCC  >  NC_000913/2770170‑2770468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: