Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,801,056 A→T 6.0% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,801,0560AT 6.0% 325.3 / 7.4 124N31I (AAT→ATT) nrdINrdEF cluster assembly flavodoxin
Reads supporting (aligned to +/- strand):  ref base A (75/41);  new base T (5/3);  total (80/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 124 total aligned reads displayed.

GGTTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGC  >  NC_000913/2800909‑2801203
                                                                                                                                                   |                                                                                                                                                   
ggtTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCCGCTCGGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATg                                                                                                                                                   >  2:499602/1‑150 (MQ=255)
ggtTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATg                                                                                                                                                   >  1:1783956/1‑150 (MQ=255)
     ccgtccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCgg                                                                                                                                              >  1:1928716/1‑150 (MQ=255)
       gtccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGa                                                                                                                                            >  2:781698/1‑150 (MQ=255)
         ccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAAc                                                                                                                                          >  1:2016032/1‑150 (MQ=255)
         ccgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGGCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAAc                                                                                                                                          <  1:1364413/150‑1 (MQ=255)
          cgGACATGATTACCCGTCTGCATCCAGCGCCACCCGCGGCCAGTGCATGAGCCAGCTCGGCTACTTCTCCAGCAGCTCCGAAACCACGCAGCGGCTTATCGAACGTTTCGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACg                                                                                                                                         <  1:296041/150‑1 (MQ=255)
          cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGatgc                                                                                                                                         <  1:705410/150‑4 (MQ=255)
          cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGatgc                                                                                                                                         <  2:719199/150‑4 (MQ=255)
          cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACg                                                                                                                                         <  1:598586/150‑1 (MQ=255)
          cgGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGACAGCTCGTCTACTTCTCCAGCAGCTACGAAAACACGCAGCGTTTTAGCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCATTGAGCGGGatgc                                                                                                                                         <  1:802477/150‑4 (MQ=255)
           ggACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCCGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACgg                                                                                                                                        >  1:1214533/1‑150 (MQ=255)
           ggACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACgg                                                                                                                                        <  2:649547/150‑1 (MQ=255)
            gACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGa                                                                                                                                       >  2:1641822/1‑150 (MQ=255)
                tGATTAACCGTCTGCATCCAGCGCCCCACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCa                                                                                                                                   >  2:1477959/1‑150 (MQ=255)
                tGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCa                                                                                                                                   <  1:1012767/150‑1 (MQ=255)
                tGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCa                                                                                                                                   <  1:117329/150‑1 (MQ=255)
                tGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCGCAATGAGCGGGAACGGAGTCa                                                                                                                                   >  1:189089/1‑150 (MQ=255)
                   ttAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGt                                                                                                                                <  1:1754902/150‑1 (MQ=255)
                     aaCCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGCACGGATTCAGGTAg                                                                                                                              >  1:157867/1‑150 (MQ=255)
                     aaCCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTATTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAg                                                                                                                              >  1:1146009/1‑150 (MQ=255)
                         gTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGCACGGATTCCGGTAGACgc                                                                                                                          >  1:989527/1‑149 (MQ=255)
                           cTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTGTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCGCAATGAGCGGGAACGGATTCAGGGAGCCGAGc                                                                                                                        >  1:1349035/1‑150 (MQ=255)
                              cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGCACGTTTAGGTCTGCCCGCGGTGAGCATCCCGCTCAATGAGCGGGATCGGATTCAGGTAGACGAGCCtt                                                                                                                     >  1:924760/1‑150 (MQ=255)
                              cATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCAGCGAAAACACGCCGCGTTTTAGCGAACGGTTAGGTCTGCCCGCGGGGCGCATCCCGCGCCATGCGCGGCACCGGACGCAGGTCGCCGCGccgt                                                                                                                     >  1:360501/1‑148 (MQ=255)
                               aTCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTa                                                                                                                    >  1:1303556/1‑150 (MQ=255)
                                tCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTAc                                                                                                                   >  1:616013/1‑150 (MQ=255)
                                 ccAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACa                                                                                                                  >  2:931506/1‑150 (MQ=255)
                                 ccAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGGTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTGGACGAGCCTTACa                                                                                                                  >  1:888906/1‑150 (MQ=255)
                                  cAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACCCGCAGCGTTTAATCGAACGTTTAGGTCTGCACGCGGTGCGCATCCCGATCAATGAGCGGGAACGGATTCAGGTAGCCGCGCCTTACAt                                                                                                                 >  2:963987/1‑150 (MQ=255)
                                  cAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACAt                                                                                                                 >  1:739231/1‑150 (MQ=255)
                                  cAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACAt                                                                                                                 >  1:520562/1‑150 (MQ=255)
                                    gcgcCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATcc                                                                                                               <  2:730304/150‑1 (MQ=255)
                                    gcgcCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATcc                                                                                                               <  2:392122/150‑1 (MQ=255)
                                       ccACACGCGGCCAGTGCATGAGCCAGCTAGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCAGCTCAATGAGGGGGATAGGATTCAGGTATACGCTCCTTACATccaca                                                                                                            >  2:196091/1‑147 (MQ=255)
                                        cacaCGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGAAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCGCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGag                                                                                                           >  1:7860/1‑149 (MQ=255)
                                          cacGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACCCGCCGCGTTTTATCGAACGTGTAGGTCTGCCCGCGGTGCGCATCCCGCGCAAGGAGCGGGAACGGATTCAGGTCGCCGAGCCTTACATCCTGATCg                                                                                                         >  1:139919/1‑150 (MQ=255)
                                          cacGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACCCGCATCGTTTTATCGCACGTTTAGGTCTGCACGCGGTGCGCATCCCGCTCAATGAGCGGGAACGCAGTCAGGTAGACGATCCTTACATCCTGTTCg                                                                                                         >  2:597329/1‑150 (MQ=255)
                                          cacGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCg                                                                                                         <  2:1928716/150‑1 (MQ=255)
                                             gcgGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGc                                                                                                      <  2:1829359/150‑1 (MQ=255)
                                               ggCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCAGCTCAATGAGCGGGAACGGATTCAGGTTGACGAGCCTTACATCCTGATCGTGccc                                                                                                    >  1:1075981/1‑150 (MQ=255)
                                                gCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGAGTCAGGTAGCCGAGCCTTACATCCTGATCGTGCCct                                                                                                   >  1:463370/1‑150 (MQ=255)
                                                  cAGTGCATTAGCAAGCTCTTCAACTAATCCAGCAGCTCCGAAAACAGGCTGCGTTTTTTAGAACGTTTAGGTCTGCCCGCCTAGCGCATCCCGCTAAATGAACGGGAACGGATTCATGTAGAAGAGCCTTAAAAACTCATCGTGACatct                                                                                                 >  2:2014734/1‑150 (MQ=255)
                                                    gTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGAGTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTa                                                                                               >  1:717516/1‑150 (MQ=255)
                                                       cATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcgg                                                                                            >  2:212757/1‑150 (MQ=255)
                                                       cATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcgg                                                                                            >  2:1387169/1‑150 (MQ=255)
                                                        aTGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGCTCGTGCCCTCTTAcggc                                                                                           >  1:1764239/1‑150 (MQ=255)
                                                        aTGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggc                                                                                           >  1:1772066/1‑150 (MQ=255)
                                                            gCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGAATAGGTCTGCCCGCGGAGCGCATCCCGCTCTATGAGAGGGAACGGATTCACGTAGACGAGCCTTACATCCAGCTCGTCCCCTCTGAcggcggcg                                                                                       >  1:280506/1‑150 (MQ=255)
                                                              cAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTAcggcggcggc                                                                                     <  2:748975/150‑1 (MQ=255)
                                                                    gTCTACTTCTCCAGCAGCACCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACg                                                                               <  1:885387/150‑1 (MQ=255)
                                                                         cTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGTGAACGGATTCAGGTAGACGACCCTTACATCCTGATCGTCCCCTCTTACGGCGGCGGCGGTACGGCTgg                                                                          >  2:697410/1‑150 (MQ=255)
                                                                           tctcCCGCAGCTCCGAAAACACGCACCGTGTTATCGACCGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGgcg                                                                        <  2:1261827/150‑1 (MQ=255)
                                                                               cagcagCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGt                                                                    <  1:1488950/150‑1 (MQ=255)
                                                                               cagcagCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGt                                                                    <  2:1287937/150‑1 (MQ=255)
                                                                               cagcagCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGt                                                                    <  1:670258/150‑1 (MQ=255)
                                                                                agcagcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTg                                                                   <  2:1679233/150‑1 (MQ=255)
                                                                                 gcagcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGc                                                                  <  1:1257084/150‑1 (MQ=255)
                                                                                    gcTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCAc                                                               >  2:1847017/1‑150 (MQ=255)
                                                                                            aaCACGCAGCGTTTTATCGAACGTTTATGTCTGCCCGCGGTGCGGATCCCGCTCAATGAGCTGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGTCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTa                                                       <  1:1450585/150‑1 (MQ=255)
                                                                                             acacGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTaa                                                      >  1:194743/1‑150 (MQ=255)
                                                                                              cacGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAAt                                                     >  1:621936/1‑150 (MQ=255)
                                                                                               ccGCAGCGTTTTATCGAACGTTTTGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGTTTTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGTAAtt                                                    <  1:963987/149‑1 (MQ=255)
                                                                                                cGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTc                                                   >  2:1507446/1‑150 (MQ=255)
                                                                                                 gCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCg                                                  >  2:436241/1‑150 (MQ=255)
                                                                                                    gCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCtt                                               <  1:238604/150‑1 (MQ=255)
                                                                                                     cGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCttt                                              <  2:712130/150‑1 (MQ=255)
                                                                                                       ttttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACAGCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCttttt                                            <  2:1214533/150‑1 (MQ=255)
                                                                                                        tttATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTa                                           >  2:1705309/1‑150 (MQ=255)
                                                                                                            tCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGTATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCTCGGTGCCACGACAGGTAATTCGCTTTTTAAacg                                       <  2:924760/150‑1 (MQ=255)
                                                                                                            tCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAacg                                       >  2:1489555/1‑150 (MQ=255)
                                                                                                             cGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTATacga                                      >  2:292706/1‑150 (MQ=255)
                                                                                                              gAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAgacgac                                     >  1:1338816/1‑150 (MQ=255)
                                                                                                              gAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAacgac                                     >  1:394272/1‑150 (MQ=255)
                                                                                                                aCGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAAcgacga                                   >  1:1159458/1‑150 (MQ=255)
                                                                                                                  gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGCGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGc                                 >  2:358184/1‑150 (MQ=255)
                                                                                                                  gTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGc                                 >  1:1643940/1‑150 (MQ=255)
                                                                                                                   tttAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGca                                >  2:200189/1‑150 (MQ=255)
                                                                                                                   tttAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGca                                <  2:1593004/150‑1 (MQ=255)
                                                                                                                         tCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCg                          >  1:1966628/1‑150 (MQ=255)
                                                                                                                          cTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCgg                         <  2:1067402/150‑1 (MQ=255)
                                                                                                                          cTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCgg                         >  1:757824/1‑150 (MQ=255)
                                                                                                                               cgcgGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGtt                    <  1:1582448/150‑1 (MQ=255)
                                                                                                                               cgcgGTGCGCATCCCGCTAAATGAGCGGGAACGGATTCAGGAAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGtt                    <  2:553729/150‑1 (MQ=255)
                                                                                                                                      ccgttCCCGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc             >  1:1488433/5‑150 (MQ=255)
                                                                                                                                      ccgttCCCGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCATCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGATTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc             >  2:54074/5‑150 (MQ=255)
                                                                                                                                       cgttCCCGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgcg            >  1:833661/4‑150 (MQ=255)
                                                                                                                                        gttCCCGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcgg           >  2:586217/3‑150 (MQ=255)
                                                                                                                                         aTCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcggc          <  1:1366682/150‑1 (MQ=255)
                                                                                                                                          tCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCgcggcg         >  1:464342/1‑150 (MQ=255)
                                                                                                                                           cccGCTCATTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGt        >  1:214210/1‑150 (MQ=255)
                                                                                                                                            ccGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGtt       >  1:1908161/1‑150 (MQ=255)
                                                                                                                                            ccGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGtt       <  1:212757/150‑1 (MQ=255)
                                                                                                                                            ccGCTCAATGAGCGGAAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGtt       >  2:1809634/1‑150 (MQ=255)
                                                                                                                                              gCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTGTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTAt     >  1:1486704/1‑150 (MQ=255)
                                                                                                                                                tCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTg   <  1:499602/150‑1 (MQ=255)
                                                                                                                                                ccAATGAGGGGGAACGGCTTCAGGGAGACGAGCCTTTCATCCTGATCGTGCCCTCGTACGGCGGCGGCGGTACGGCTGGGGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTg   <  2:1799677/149‑1 (MQ=255)
                                                                                                                                                 cAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTgg  >  2:875154/1‑149 (MQ=255)
                                                                                                                                                 cAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGc  >  2:1185704/1‑150 (MQ=255)
                                                                                                                                                 cAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGc  >  1:1607667/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTTTCCGTCCGGACATGATTAACCGTCTGCATCCAGCGCCACACGCGGCCAGTGCATGAGCCAGCTCGTCTACTTCTCCAGCAGCTCCGAAAACACGCAGCGTTTTATCGAACGTTTAGGTCTGCCCGCGGTGCGCATCCCGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGC  >  NC_000913/2800909‑2801203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑