Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,807,093 A→T 6.9% intergenic (+19/‑39) proW → / → proX glycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,0930AT 6.9% 331.4 / 10.2 123intergenic (+19/‑39)proW/proXglycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base A (52/58);  new base T (5/4);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 124 total aligned reads displayed.

TGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACC  >  NC_000913/2806948‑2807242
                                                                                                                                                 |                                                                                                                                                     
tgtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGcc                                                                                                                                                   <  1:449945/150‑1 (MQ=255)
  tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGCCGCCgg                                                                                                                                                 >  1:1889283/1‑150 (MQ=255)
  tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                                                                                                                                                 >  1:1304584/1‑150 (MQ=255)
    aTCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGc                                                                                                                                               >  1:884191/1‑150 (MQ=255)
     tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGTTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                                                                              <  1:850087/150‑1 (MQ=255)
     tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCa                                                                                                                                              <  2:54050/150‑1 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGGCGTGGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                         <  2:1927786/150‑1 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                         >  1:528116/1‑150 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgc                                                                                                                                         <  1:1771106/150‑6 (MQ=255)
           ccATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCa                                                                                                                                        <  2:1063428/150‑1 (MQ=255)
             aTTATCCTCGATCGTCTGACGCAGGCCGTGGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGCCCCTGTTGGTCTGCTGACCCGCCCATTCATAAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAcc                                                                                                                                      <  1:603310/150‑1 (MQ=255)
              ttATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAccc                                                                                                                                     <  1:1109793/150‑1 (MQ=255)
               tctCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTCACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCt                                                                                                                                    <  1:1146317/148‑1 (MQ=255)
                aTCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTg                                                                                                                                   <  1:675096/150‑1 (MQ=255)
                  ccTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGcc                                                                                                                                 >  2:940269/1‑150 (MQ=255)
                   cTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCAGTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCa                                                                                                                                >  1:1109714/1‑150 (MQ=255)
                       aTCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaa                                                                                                                            <  1:1257447/150‑1 (MQ=255)
                       aTCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaa                                                                                                                            <  1:1877739/150‑1 (MQ=255)
                             tGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGaa                                                                                                                      >  2:247529/1‑150 (MQ=255)
                                  cAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACa                                                                                                                 <  2:822996/150‑1 (MQ=255)
                                   aGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACCCCACTGGCCCTGTTGGTCTGCTGACCCGCCCAGTCTTTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAGCCGGCCAAAAAAAGGAATAACaa                                                                                                                >  1:2018847/1‑150 (MQ=255)
                                           gggCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAt                                                                                                        <  2:680808/150‑1 (MQ=255)
                                             gcgcGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCGCCCTGCAAAAAAAAGGAATAAAAATGAGACATAg                                                                                                      >  1:1458425/1‑150 (MQ=255)
                                               gcgACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCg                                                                                                    <  1:1197106/150‑1 (MQ=255)
                                                    tCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGGATTATCCCCCTGCCAAAAAAAGGAATAACAATGCGACAGAGCGTACtt                                                                                               >  1:1849864/1‑150 (MQ=255)
                                                     cccGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGGAACTGTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACCATGCGACAGAGCGTACttt                                                                                              >  2:307210/3‑150 (MQ=255)
                                                     cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttt                                                                                              >  2:1872615/1‑150 (MQ=255)
                                                     cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAGAGCGTACttt                                                                                              >  1:63584/1‑150 (MQ=255)
                                                       cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttttt                                                                                            >  2:406984/1‑150 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                          <  2:1031604/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                          <  2:38242/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                          <  1:1426740/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGAGGGTACACCACTGGCCCTGTTGGTCTGCTGAACCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                          <  1:1231549/150‑1 (MQ=255)
                                                          aGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCg                                                                                         <  1:1713790/150‑1 (MQ=255)
                                                                ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCg                                                                                   <  2:236109/150‑1 (MQ=255)
                                                                    aCCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTg                                                                               <  2:1633570/150‑1 (MQ=255)
                                                                      cggcGCTGGTACCCCACGGGCCCTGTTGGTCTGCTGACCCGCCAATTCATTCAGTAACTCTGCACTTTCCCGGGGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                             <  2:234479/147‑1 (MQ=255)
                                                                      cGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATCATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                             >  1:1840801/1‑150 (MQ=255)
                                                                       gTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTCCTTTTTGCGACAGCGTTTGCCa                                                                            >  2:1821271/1‑150 (MQ=255)
                                                                        gcGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCAc                                                                           <  1:54447/149‑1 (MQ=255)
                                                                          gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                         >  2:1893262/1‑150 (MQ=255)
                                                                          gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                         <  1:845557/150‑1 (MQ=255)
                                                                          gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                         >  2:1635083/1‑150 (MQ=255)
                                                                           cTGGTACACCTCTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAGCTCTGCACTTGCCAGGTGCCGCCGGGCATTATCACCCTGACAAAAAAAGGAATAACAATCCGCCATAGCGTACTTTTTGCGACAGCGTTTGCCCCGcc                                                                        >  1:185185/1‑149 (MQ=255)
                                                                           cTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                                                                        <  1:1871197/150‑1 (MQ=255)
                                                                             ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                      >  2:1317082/1‑150 (MQ=255)
                                                                               tACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                                                                    <  1:1978830/150‑1 (MQ=255)
                                                                                  accacTGGCCCTGTTGGTCTGCTGACCCGGCGATTCATTAAGTAACTCTGCTCTTGCCCGGTGACGCCGGGCATTATCAGACTGCCATAAAATGGAATAGCAATGCGACTTAGCGTAGTGTTTGCGTCAGCGTTTGCGACGCTTATctct                                                                 >  1:300824/1‑150 (MQ=255)
                                                                                  accacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATctct                                                                 <  1:884282/150‑1 (MQ=255)
                                                                                   ccacTGGCCCTGTTGGTCTGCGGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGTAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTa                                                                <  2:1765685/150‑1 (MQ=255)
                                                                                      cTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTacac                                                             <  2:679705/150‑1 (MQ=255)
                                                                                        ggCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATACCAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACaa                                                           >  2:795620/1‑150 (MQ=255)
                                                                                           ccTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACt                                                        <  1:1397138/150‑1 (MQ=255)
                                                                                           ccTGTTGGTCTGCTGACCCGACCATTCATTAAGTAACTCTGCACTTGCCCGGTGCCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGAGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACt                                                        >  1:152689/1‑150 (MQ=255)
                                                                                               ttGGTCTGCTGACCCGCTCATTCATTAAGTAACTCTGCACTTGCCCGGGGTCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtg                                                    <  2:927847/150‑1 (MQ=255)
                                                                                               ttGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtg                                                    <  1:1226324/150‑1 (MQ=255)
                                                                                                tGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgc                                                   <  2:1109714/150‑1 (MQ=255)
                                                                                                tGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgc                                                   <  2:1304584/150‑1 (MQ=255)
                                                                                                   tCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGTGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctgc                                                >  2:818178/1‑150 (MQ=255)
                                                                                                   tCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctgc                                                <  1:1570477/150‑1 (MQ=255)
                                                                                                    ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGcc                                               >  2:1498840/1‑150 (MQ=255)
                                                                                                         gACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCt                                          >  1:465058/1‑150 (MQ=255)
                                                                                                           cccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAGGGATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGcc                                       >  2:689758/1‑150 (MQ=255)
                                                                                                            ccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGcc                                       <  1:829977/150‑1 (MQ=255)
                                                                                                             cGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTTCCg                                      >  2:1265500/1‑150 (MQ=255)
                                                                                                              gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCAGCCAAAAAAAGGAATAACAATGCGACATAGCGTACATTTTGCGACAGCGTTTGACACGCTTATCACTACACAAACTTTTGCTGCCTATCTGCCgg                                     >  2:1530436/1‑150 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                    <  2:1835643/150‑1 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                    <  2:1261527/150‑1 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAAATCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                    <  2:1907317/150‑1 (MQ=255)
                                                                                                                 cattcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCTTTATCACCCTGCCAAAAAACGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGTCa                                  <  2:1779607/150‑1 (MQ=255)
                                                                                                                 cattcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCa                                  >  2:1043741/1‑150 (MQ=255)
                                                                                                                   ttcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGATGCCGATCTGCCGGGCaaa                                >  2:894208/1‑150 (MQ=255)
                                                                                                                    tcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAg                               <  2:1411303/150‑1 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                              <  1:1949789/150‑1 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                              >  2:1005661/1‑150 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                              >  2:658584/1‑150 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                              >  2:1968177/1‑150 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGAGTTTGCCACGCATATCTCTACACAAACTTTTGCTGCCGATCTGCCGGCCAAAgg                              >  2:569135/1‑150 (MQ=255)
                                                                                                                       ttAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCa                            <  1:919181/150‑1 (MQ=255)
                                                                                                                              aCTCTGCACTTGCCGGGTGCCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGt                     <  2:2018847/150‑1 (MQ=255)
                                                                                                                                 gtgataatGCCCGGCGTCACCGTGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAACCTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa                  >  2:1226465/8‑150 (MQ=255)
                                                                                                                                 ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa                  >  1:2010398/1‑150 (MQ=255)
                                                                                                                                  tGCACTTGCCCGGTGACGCCGTGCATTATCACCCTGCCAAAAAAAGGATTAACAATGCGACATAGCGTACTTTTTGCGACAGCGGTTGCCACCCTTATCTCTACACAAACTTTTGCTGCCGATCTGACTGGCATAGGCATTACTGTTAAt                 >  1:335715/1‑150 (MQ=255)
                                                                                                                                  tGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAAt                 <  1:1178002/150‑1 (MQ=255)
                                                                                                                                  tGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAAt                 <  1:1043968/150‑1 (MQ=255)
                                                                                                                                   gataatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc                >  1:1597362/6‑150 (MQ=255)
                                                                                                                                   gCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc                <  1:923165/150‑1 (MQ=255)
                                                                                                                                    cACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATcc               <  2:884191/150‑1 (MQ=255)
                                                                                                                                    cACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACGTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATcc               >  2:1618825/1‑150 (MQ=255)
                                                                                                                                     taatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACCGCGTTTGCCACGCTTATCTCTACCCAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCa              >  1:1740557/4‑150 (MQ=255)
                                                                                                                                      cTTGCCCGGTGACGCCGGGCATTATCACCCAGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAg             <  2:1458425/150‑1 (MQ=255)
                                                                                                                                        tGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGATGCCGATCTGCCGGGGAAAGGCATTACTTTTAATCCAGtt           >  2:1256293/1‑150 (MQ=255)
                                                                                                                                        tGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTCCTGTTAATCCAGtt           >  1:205660/1‑150 (MQ=255)
                                                                                                                                         gaccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTc          <  2:1849864/148‑1 (MQ=255)
                                                                                                                                         gCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTc          <  1:247529/150‑1 (MQ=255)
                                                                                                                                          cccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCa         >  2:816262/1‑150 (MQ=255)
                                                                                                                                          cccGGTGACGCCGGGCATTATCACCCTGCAAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCa         >  1:1761693/1‑150 (MQ=255)
                                                                                                                                           ccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCag        <  1:18313/150‑1 (MQ=255)
                                                                                                                                                gACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCAc   >  2:1319060/1‑150 (MQ=255)
                                                                                                                                                 aCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCAcc  <  1:1317082/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACC  >  NC_000913/2806948‑2807242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: