Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,807,095 G→A 6.2% intergenic (+21/‑37) proW → / → proX glycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,0950GA 6.2% 401.7 / 8.5 127intergenic (+21/‑37)proW/proXglycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base G (58/61);  new base A (5/3);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCA  >  NC_000913/2806948‑2807243
                                                                                                                                                   |                                                                                                                                                    
tgtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGcc                                                                                                                                                    <  1:449945/150‑1 (MQ=255)
  tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGCCGCCgg                                                                                                                                                  >  1:1889283/1‑150 (MQ=255)
  tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                                                                                                                                                  >  1:1304584/1‑150 (MQ=255)
   gATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCggg                                                                                                                                                 >  2:2219/1‑150 (MQ=255)
    aTCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGc                                                                                                                                                >  1:884191/1‑150 (MQ=255)
     tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGTTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                                                                               <  1:850087/150‑1 (MQ=255)
      ccTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCGCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAt                                                                                                                                              >  1:927847/1‑150 (MQ=255)
       cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTACGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                             <  1:1298977/150‑3 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGGCGTGGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                          <  2:1927786/150‑1 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                          >  1:528116/1‑150 (MQ=255)
          gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgc                                                                                                                                          <  1:1771106/150‑6 (MQ=255)
           ccATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCa                                                                                                                                         <  2:1063428/150‑1 (MQ=255)
              ttATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAccc                                                                                                                                      <  1:1109793/150‑1 (MQ=255)
               tctCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTCACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCt                                                                                                                                     <  1:1146317/148‑1 (MQ=255)
                aTCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTg                                                                                                                                    <  1:675096/150‑1 (MQ=255)
                  ccTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGcc                                                                                                                                  >  2:940269/1‑150 (MQ=255)
                   cTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCAGTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCa                                                                                                                                 >  1:1109714/1‑150 (MQ=255)
                       aTCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaa                                                                                                                             <  1:1877739/150‑1 (MQ=255)
                          gTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAg                                                                                                                          >  1:1624204/1‑150 (MQ=255)
                                  cAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACa                                                                                                                  <  2:822996/150‑1 (MQ=255)
                                   aGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACCCCACTGGCCCTGTTGGTCTGCTGACCCGCCCAGTCTTTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAGCCGGCCAAAAAAAGGAATAACaa                                                                                                                 >  1:2018847/1‑150 (MQ=255)
                                   aGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACaa                                                                                                                 >  2:585056/1‑150 (MQ=255)
                                           gggCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAt                                                                                                         <  2:680808/150‑1 (MQ=255)
                                             gcgcGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCGCCCTGCAAAAAAAAGGAATAAAAATGAGACATAg                                                                                                       >  1:1458425/1‑150 (MQ=255)
                                               gcgACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCg                                                                                                     <  1:1197106/150‑1 (MQ=255)
                                                  aCTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTAc                                                                                                  <  1:1824467/150‑1 (MQ=255)
                                                    tCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGGATTATCCCCCTGCCAAAAAAAGGAATAACAATGCGACAGAGCGTACtt                                                                                                >  1:1849864/1‑150 (MQ=255)
                                                     cccGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGGAACTGTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACCATGCGACAGAGCGTACttt                                                                                               >  2:307210/3‑150 (MQ=255)
                                                     cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttt                                                                                               >  2:1872615/1‑150 (MQ=255)
                                                     cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAGAGCGTACttt                                                                                               >  1:63584/1‑150 (MQ=255)
                                                       cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttttt                                                                                             >  2:406984/1‑150 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                           <  2:718241/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                           <  1:1426740/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                           <  2:38242/150‑1 (MQ=255)
                                                         cAGTCGCGGCAACCGTCGAGGGTACACCACTGGCCCTGTTGGTCTGCTGAACCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                                                                           <  1:1231549/150‑1 (MQ=255)
                                                          aGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCg                                                                                          <  1:1713790/150‑1 (MQ=255)
                                                                ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCg                                                                                    <  2:236109/150‑1 (MQ=255)
                                                                    aCCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTg                                                                                <  2:1633570/150‑1 (MQ=255)
                                                                     ccGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGc                                                                               >  1:640908/1‑150 (MQ=255)
                                                                      cggcGCTGGTACCCCACGGGCCCTGTTGGTCTGCTGACCCGCCAATTCATTCAGTAACTCTGCACTTTCCCGGGGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                              <  2:234479/147‑1 (MQ=255)
                                                                      cGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                              >  1:493245/1‑150 (MQ=255)
                                                                      cGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATCATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                              >  1:1840801/1‑150 (MQ=255)
                                                                       gTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTCCTTTTTGCGACAGCGTTTGCCa                                                                             >  2:1821271/1‑150 (MQ=255)
                                                                        gcGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCAc                                                                            <  1:54447/149‑1 (MQ=255)
                                                                         cGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGCCAGCGTTTGCCACg                                                                           >  1:26759/1‑150 (MQ=255)
                                                                          gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                          >  2:1635083/1‑150 (MQ=255)
                                                                          gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                          >  2:1893262/1‑150 (MQ=255)
                                                                           cTGGTACACCTCTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAGCTCTGCACTTGCCAGGTGCCGCCGGGCATTATCACCCTGACAAAAAAAGGAATAACAATCCGCCATAGCGTACTTTTTGCGACAGCGTTTGCCCCGcc                                                                         >  1:185185/1‑149 (MQ=255)
                                                                           cTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                                                                         <  1:1871197/150‑1 (MQ=255)
                                                                             ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                       >  2:1317082/1‑150 (MQ=255)
                                                                                acacCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtct                                                                    <  2:837017/150‑1 (MQ=255)
                                                                                  accacTGGCCCTGTTGGTCTGCTGACCCGGCGATTCATTAAGTAACTCTGCTCTTGCCCGGTGACGCCGGGCATTATCAGACTGCCATAAAATGGAATAGCAATGCGACTTAGCGTAGTGTTTGCGTCAGCGTTTGCGACGCTTATctct                                                                  >  1:300824/1‑150 (MQ=255)
                                                                                  accacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATctct                                                                  <  1:884282/150‑1 (MQ=255)
                                                                                   ccacTGGCCCTGTTGGTCTGCGGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGTAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTa                                                                 <  2:1765685/150‑1 (MQ=255)
                                                                                     acTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTaca                                                               <  2:1282606/150‑1 (MQ=255)
                                                                                               ttGGTCTGCTGACCCGCTCATTCATTAAGTAACTCTGCACTTGCCCGGGGTCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtg                                                     <  2:927847/150‑1 (MQ=255)
                                                                                               ttGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtg                                                     <  1:1226324/150‑1 (MQ=255)
                                                                                                tGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgc                                                    <  2:1109714/150‑1 (MQ=255)
                                                                                                   tCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGTGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctgc                                                 >  2:818178/1‑150 (MQ=255)
                                                                                                   tCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctgc                                                 <  1:1570477/150‑1 (MQ=255)
                                                                                                   tCTGCTGACACGCCCATTCATTAAGTAACTCTGCACTTGACCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctgc                                                 <  2:1211679/150‑1 (MQ=255)
                                                                                                    ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGcc                                                >  2:1498840/1‑150 (MQ=255)
                                                                                                           cccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAGGGATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGcc                                        >  2:689758/1‑150 (MQ=255)
                                                                                                            ccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGcc                                        <  1:829977/150‑1 (MQ=255)
                                                                                                             cGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTTCCg                                       >  2:1265500/1‑150 (MQ=255)
                                                                                                              gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCAGCCAAAAAAAGGAATAACAATGCGACATAGCGTACATTTTGCGACAGCGTTTGACACGCTTATCACTACACAAACTTTTGCTGCCTATCTGCCgg                                      >  2:1530436/1‑150 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                     <  2:1261527/150‑1 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                     <  2:1835643/150‑1 (MQ=255)
                                                                                                               cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAAATCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                     <  2:1907317/150‑1 (MQ=255)
                                                                                                                 cattcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCTTTATCACCCTGCCAAAAAACGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGTCa                                   <  2:1779607/150‑1 (MQ=255)
                                                                                                                 cattcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCa                                   >  2:1043741/1‑150 (MQ=255)
                                                                                                                   ttcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGATGCCGATCTGCCGGGCaaa                                 >  2:894208/1‑150 (MQ=255)
                                                                                                                    tcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAg                                <  2:1411303/150‑1 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                               <  1:1949789/150‑1 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                               >  2:658584/1‑150 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                               >  2:1005661/1‑150 (MQ=255)
                                                                                                                     cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGAGTTTGCCACGCATATCTCTACACAAACTTTTGCTGCCGATCTGCCGGCCAAAgg                               >  2:569135/1‑150 (MQ=255)
                                                                                                                           gTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTAc                         <  1:14386/150‑1 (MQ=255)
                                                                                                                              aCTCTGCACTTGCCGGGTGCCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGt                      <  2:2018847/150‑1 (MQ=255)
                                                                                                                               ctctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGtt                     >  1:1185311/1‑150 (MQ=255)
                                                                                                                                 ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGTCACGCTTATCTCTACACAGACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa                   >  2:363419/1‑150 (MQ=255)
                                                                                                                                 ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa                   >  1:2010398/1‑150 (MQ=255)
                                                                                                                                  tGCACTTGCCCGGTGACGCCGTGCATTATCACCCTGCCAAAAAAAGGATTAACAATGCGACATAGCGTACTTTTTGCGACAGCGGTTGCCACCCTTATCTCTACACAAACTTTTGCTGCCGATCTGACTGGCATAGGCATTACTGTTAAt                  >  1:335715/1‑150 (MQ=255)
                                                                                                                                  tGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAAt                  <  1:1043968/150‑1 (MQ=255)
                                                                                                                                   gataatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc                 >  1:1597362/6‑150 (MQ=255)
                                                                                                                                   gCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc                 <  1:923165/150‑1 (MQ=255)
                                                                                                                                    cACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACGTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATcc                >  2:1618825/1‑150 (MQ=255)
                                                                                                                                     taatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATTACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCa               >  1:877599/4‑150 (MQ=255)
                                                                                                                                      cTTGCCCGGTGACGCCGGGCATTATCACCCAGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAg              <  2:1458425/150‑1 (MQ=255)
                                                                                                                                        tGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGATGCCGATCTGCCGGGGAAAGGCATTACTTTTAATCCAGtt            >  2:1256293/1‑150 (MQ=255)
                                                                                                                                        tGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTCCTGTTAATCCAGtt            >  1:205660/1‑150 (MQ=255)
                                                                                                                                         gaccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTc           <  2:1849864/148‑1 (MQ=255)
                                                                                                                                          cccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCa          >  2:816262/1‑150 (MQ=255)
                                                                                                                                          cccGGTGACGCCGGGCATTATCACCCTGCAAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCa          >  1:1761693/1‑150 (MQ=255)
                                                                                                                                           ccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCag         >  1:1983256/1‑150 (MQ=255)
                                                                                                                                                gACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCAc    >  2:1319060/1‑150 (MQ=255)
                                                                                                                                                 aCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCAcc   <  1:1317082/150‑1 (MQ=255)
                                                                                                                                                  cGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCa  >  2:686320/1‑150 (MQ=255)
                                                                                                                                                  cGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCa  >  1:1758166/1‑150 (MQ=255)
                                                                                                                                                  cGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGAGTTTGCCACGCTTATCTCTAAACAAAATTTTGCTGCCGATCTGCCTGGCAAAGGAATTACTGTTAATCCAGTTCAGAGCACCa  >  1:1373146/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCACCA  >  NC_000913/2806948‑2807243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: