Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,861 C→T 5.5% noncoding (60/78 nt) micA → sRNA antisense regulator of ompA, lamB, ompX, and phoP; Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,8610CT 5.5% 397.3 / 8.0 127noncoding (60/78 nt)micAsRNA antisense regulator of ompA, lamB, ompX, and phoP; Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (58/61);  new base T (3/4);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 127 total aligned reads displayed.

GCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCT  >  NC_000913/2814716‑2815009
                                                                                                                                                 |                                                                                                                                                    
gCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt                                                                                                                                                  >  1:1974011/1‑150 (MQ=255)
      tAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGTGAGTGGccaa                                                                                                                                            <  1:876734/150‑3 (MQ=255)
       aacaacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGTGAGTGGccaaa                                                                                                                                           <  2:1367322/150‑4 (MQ=255)
         caacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                                                                                                         >  1:180223/1‑150 (MQ=255)
         caacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttt                                                                                                                                         >  1:542060/1‑150 (MQ=255)
          aacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCttttta                                                                                                                                        >  1:902305/1‑149 (MQ=255)
          aacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttc                                                                                                                                        >  1:1250820/1‑150 (MQ=255)
          aacGGCATTTAGCCACCTCCGGTAATTTTTTCAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGTGAGTGGccaaaatt                                                                                                                                        <  2:720508/150‑7 (MQ=255)
           acGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttct                                                                                                                                       >  1:328104/1‑150 (MQ=255)
              gCATTTATCCACCTCCGGTATTTTTTTTAAATATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCTTTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttctttt                                                                                                                                    <  2:795116/150‑1 (MQ=255)
               cATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTCTATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                                                                                                                   <  2:1860726/150‑1 (MQ=255)
               cATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                                                                                                                   >  1:1454145/1‑150 (MQ=255)
               cATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCATTCTTCCCATGCGAGTCTGAGTATATGAAAGACGCGCATTTTTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACAAGTGGCCTttttcttttc                                                                                                                                   <  1:603164/150‑1 (MQ=255)
               cATTTAGCCACCTCCGGTAATTTTTTTAAAAATTGTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttttc                                                                                                                                   <  1:1812690/150‑1 (MQ=255)
                 tttAGCCACCTCCGGTATTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCCGGCTAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGATTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTg                                                                                                                                 <  1:168572/150‑1 (MQ=255)
                 tttAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTg                                                                                                                                 >  2:1991957/1‑150 (MQ=255)
                   tAGCCACGTCCGGTAATTTTTTTAAAAATTTTCTGATCTCTTTCTTCCCAGGCGCGGCTGAGTATATGAAAGACGCGGATTTGTTATCATGATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGta                                                                                                                               <  1:129320/150‑2 (MQ=255)
                    aGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                                                                                                              <  2:1258369/150‑1 (MQ=255)
                    aGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                                                                                                              <  1:1049548/150‑1 (MQ=255)
                    aGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                                                                                                              <  1:1430264/150‑1 (MQ=255)
                     gCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGCCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTTGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAg                                                                                                                             <  2:1421908/150‑1 (MQ=255)
                      ccACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                                                                                                            <  1:89235/150‑1 (MQ=255)
                       cACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGc                                                                                                                           >  2:5094/1‑150 (MQ=255)
                          cTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg                                                                                                                        <  1:435664/150‑1 (MQ=255)
                                 aaTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcc                                                                                                                 <  2:888208/150‑1 (MQ=255)
                                   tttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTAAATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAg                                                                                                               <  2:861830/150‑1 (MQ=255)
                                   tttttttAAAAATTTTATGAAATCTATCATTCCAGGCGAGTGTGAGTTTATGAAATACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAg                                                                                                               <  2:910653/150‑1 (MQ=255)
                                    ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGATTTTCCAGc                                                                                                              >  1:1616117/1‑150 (MQ=255)
                                    ttttttAAAAATTTTCTGAACTCTTTCTTCCAAGGCGAGTCTGAGTATATTAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGc                                                                                                              <  1:691370/150‑1 (MQ=255)
                                     tttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGcc                                                                                                             >  1:632667/1‑150 (MQ=255)
                                     tttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGcc                                                                                                             <  1:1383695/150‑1 (MQ=255)
                                       tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac                                                                                                           >  1:1244424/1‑150 (MQ=255)
                                          aaaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCATATTTCTTTTCTGTCAGGCGTGATTTTCCAGCCaaaca                                                                                                        >  1:1870299/1‑149 (MQ=255)
                                          aaaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAAATCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACAcc                                                                                                        >  1:84048/1‑150 (MQ=255)
                                           aaaaTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCg                                                                                                       <  2:593083/150‑1 (MQ=255)
                                             aaTTTTCTGAACTCTTTCTTCCCAGGCGAGGCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCa                                                                                                     <  1:1279965/150‑1 (MQ=255)
                                                   cTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGt                                                                                               >  1:488285/1‑150 (MQ=255)
                                                    tGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCAAGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGtt                                                                                              >  2:575439/1‑150 (MQ=255)
                                                     gAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTc                                                                                             >  2:165513/1‑150 (MQ=255)
                                                       aCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCgg                                                                                           <  1:1382215/150‑1 (MQ=255)
                                                        ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGt                                                                                          >  1:1955864/1‑150 (MQ=255)
                                                         tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTa                                                                                         <  2:875001/150‑1 (MQ=255)
                                                          ctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTAt                                                                                        >  1:1462990/1‑150 (MQ=255)
                                                           tttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTAta                                                                                       >  1:484098/1‑149 (MQ=255)
                                                              cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTGTTTTCTGTCAGGCGTGTTTTTCCCGCCACACCGCAAACGGGTCGGTATCAGc                                                                                    >  2:1762943/1‑150 (MQ=255)
                                                                   cAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCtt                                                                               >  2:1800531/1‑150 (MQ=255)
                                                                   cAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCtt                                                                               >  1:1449763/1‑150 (MQ=255)
                                                                     ggCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTcc                                                                             >  2:481293/1‑150 (MQ=255)
                                                                      gCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCa                                                                            <  2:213783/150‑1 (MQ=255)
                                                                         aGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGAATCAGCGGCTTCCAttt                                                                         <  2:1683458/150‑1 (MQ=255)
                                                                          gTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTc                                                                        >  2:407618/1‑150 (MQ=255)
                                                                           tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTcc                                                                       <  2:182237/150‑1 (MQ=255)
                                                                            cTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCct                                                                      <  2:1011057/150‑1 (MQ=255)
                                                                            cTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTCAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCct                                                                      <  1:1826196/150‑1 (MQ=255)
                                                                                  atatGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGAc                                                                <  2:1250820/150‑1 (MQ=255)
                                                                                   tatGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACg                                                               >  2:33996/1‑150 (MQ=255)
                                                                                   tatGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACg                                                               >  2:662822/1‑150 (MQ=255)
                                                                                    atGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACgg                                                              >  2:1107219/1‑150 (MQ=255)
                                                                                     tGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGc                                                             <  2:328104/150‑1 (MQ=255)
                                                                                     tGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCGGTCAGGCGTGTTTTTCCAGCCCCACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGc                                                             >  2:95530/1‑150 (MQ=255)
                                                                                         agtcgcgCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTCTTTTTCCAGCCTCACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTc                                                         <  2:573675/147‑1 (MQ=255)
                                                                                         aGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTc                                                         <  2:1244424/150‑1 (MQ=255)
                                                                                          gACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCa                                                        <  1:1864649/150‑1 (MQ=255)
                                                                                          gACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCa                                                        >  1:1699716/1‑150 (MQ=255)
                                                                                            cgcgCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTATTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGa                                                      >  2:781670/1‑150 (MQ=255)
                                                                                             gcgcATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGAc                                                     <  2:929603/150‑1 (MQ=255)
                                                                                                cATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCGTGCTGACGGCGTTCAGACGcc                                                  >  1:940696/1‑150 (MQ=255)
                                                                                                      ttATCATCATCCCTGAATTCATAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCgcgc                                            <  2:1663019/150‑1 (MQ=255)
                                                                                                         tcatcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCAACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCg                                         >  1:471310/1‑150 (MQ=255)
                                                                                                          catcatcCCTGAATTCAGAGATGAGATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                        >  2:473724/1‑150 (MQ=255)
                                                                                                          catcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                        >  2:588605/1‑150 (MQ=255)
                                                                                                          catcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                        <  1:979035/150‑1 (MQ=255)
                                                                                                           atcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGGTCAGACGCCTCGCGCTCGGc                                       <  2:474882/150‑1 (MQ=255)
                                                                                                            tcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCt                                      <  2:1368580/150‑1 (MQ=255)
                                                                                                             catcCCTGAATTCCGACATGACATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTa                                     <  2:481437/150‑1 (MQ=255)
                                                                                                              atcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCGTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTAc                                    >  1:1163094/1‑150 (MQ=255)
                                                                                                               tcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACTGCGTTCAGACGCCTCGCGCTCGGCTACa                                   >  2:1798993/1‑150 (MQ=255)
                                                                                                               tcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACa                                   >  1:222798/1‑150 (MQ=255)
                                                                                                                cccTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACaa                                  >  2:534022/1‑150 (MQ=255)
                                                                                                                     aaTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCTGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATcc                             <  2:243080/150‑1 (MQ=255)
                                                                                                                     aaTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATcc                             <  1:794382/150‑1 (MQ=255)
                                                                                                                     aaTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTCTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATcc                             <  2:1411437/150‑1 (MQ=255)
                                                                                                                        tCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCctct                          >  2:1553370/1‑150 (MQ=255)
                                                                                                                         cAGAGATGAAATTTTGGCCACGCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCACACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTTCACTATCCTCtt                         >  1:1177038/1‑150 (MQ=255)
                                                                                                                           gagaTGAAATTTTGGCCACTCACGAGTGGCCATTTTCTTTTCTGTCCGGCGTGTTTTTCCAGCCACCCCGCACACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGCCGGCGTGCAGACGCCTCTCGCGGGGCTACAAAAACCtcgtcg                       >  1:88263/1‑150 (MQ=255)
                                                                                                                            agaTGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTcgc                      <  2:387600/150‑1 (MQ=255)
                                                                                                                              aTGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAATGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCgcgc                    <  2:180223/150‑1 (MQ=255)
                                                                                                                              aTGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCgcgc                    >  1:1856260/1‑150 (MQ=255)
                                                                                                                               tGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCa                   <  1:1800531/150‑1 (MQ=255)
                                                                                                                                gAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAg                  <  2:1454145/150‑1 (MQ=255)
                                                                                                                                 aaaTTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGa                 >  1:104450/1‑150 (MQ=255)
                                                                                                                                     aaaggCCACTCGTGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTTCAAAATCCTCTTCGCGCAGAAttt             >  1:1128112/4‑150 (MQ=255)
                                                                                                                                        ggCCACTCGTGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCa          >  2:941651/1‑150 (MQ=255)
                                                                                                                                         gCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACCCCGCCAACGGTGCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCCGACGCCTCGCGCGGGGCGACGATCTCCTCTTCGCGCAGAATTTCCAg         >  2:92360/1‑150 (MQ=255)
                                                                                                                                         gCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAg         >  2:782426/1‑150 (MQ=255)
                                                                                                                                         gCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGCGTTTTTCAAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACCGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGACTTTCCAg         >  1:1553593/1‑150 (MQ=255)
                                                                                                                                            aCTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCgg      <  2:1362808/150‑1 (MQ=255)
                                                                                                                                             cTCACGAGTGGACTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGCCGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCATCTTCGAGCAGAATTTCCAGCGGc     >  2:1375524/1‑150 (MQ=255)
                                                                                                                                               cACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCGGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGctc   >  1:728371/1‑150 (MQ=255)
                                                                                                                                               cACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGctc   <  2:665879/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCT  >  NC_000913/2814716‑2815009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: