BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,324	G→A	8.3%	intergenic (‑50/+149)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,324	0	G	A	8.3%	294.4 / 11.6	111	intergenic (‑50/+149)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (74/24); new base A (5/4); total (79/32)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATAT  >  NC_000913/2818180‑2818473

gCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCCAACCTCCGACCGAACGGGTCCTAGCCGAGTAGTCTATCCAGCTGAGCTCCGTCTGCAACGGGAAACTTAC‑GTTACTGCAGAGTTTTTGAGACCGGTCGTAACGCCGTTTCa                                                                                                                                                  >  1:794397/1‑150 (MQ=255)

      tCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAag                                                                                                                                            >  1:649610/1‑150 (MQ=255)

      tCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAag                                                                                                                                            >  1:937068/1‑150 (MQ=255)

            cgaataTGGTGCATCCGGGAGGCTTCGAACCTCCGCCCGCGCGGGTCGTAGGCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATgg                                                                                                                                      <  2:305077/145‑1 (MQ=14)

             agagATGGTGCATCCGGGAGGATTCGAACCAAAGACCCAACCCTTCCTAGCCGAGTACTCTATACAGATGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCAGTATCAAGTAAGAGATGGt                                                                                                                                     >  2:450658/1‑150 (MQ=17)

                           cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGAt                                                                                                                       >  2:1043547/1‑150 (MQ=255)

                             ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTa                                                                                                                     >  1:1368829/1‑150 (MQ=255)

                                 gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCg                                                                                                                 <  1:1449567/150‑1 (MQ=255)

                                  aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGc                                                                                                                >  1:2009558/1‑150 (MQ=255)

                                   ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGATACTAACGCCGTATAAAGTAAGAGATGGTGAATCCGGGAGGATTACTCGcc                                                                                                               >  2:970610/1‑150 (MQ=255)

                                        aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAAGGGATAGTTAC‑TATTCTGCAGATTTTTTGATACCGATACTAAAGCGGTATCAAGTTAGAGATGGTGGATCAGGGAGGATTACTCGCCTTGCg                                                                                                          >  2:674958/1‑150 (MQ=17)

                                         ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACGTAC‑TTTACTGCAGATTTTTTGATGCCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                         >  1:1136208/1‑150 (MQ=255)

                                           tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCTGGAAAATAAC‑ATTCCTGAAGATTAAATGATTCCGCTATTAAACCCATATAAAGTAACACTTGGTGAATCCTGGAGGATTACTCGCCAGGCGGCt                                                                                                       >  2:1786858/1‑150 (MQ=9)

                                             cGACCGCTATGTTCGTAGCCGTTTAATCTATCCAGCTTAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGTTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                     <  2:1509843/150‑1 (MQ=17)

                                              gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTAGc                                                                                                    >  2:1036144/1‑150 (MQ=255)

                                                  gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCtt                                                                                                >  2:1000797/1‑150 (MQ=255)

                                                   cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCATc                                                                                               >  2:1745361/1‑150 (MQ=255)

                                                     cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                             >  1:403687/1‑150 (MQ=255)

                                                             tctcGAGTATTCTATCCAGCTGAGCTACCGATTTCTAGGGTAACTTAT‑TTTATTGAAGATTTTTTGATACCGCTACTAAAGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                     <  2:1685105/147‑1 (MQ=17)

                                                                  tgTACTCTATTCTGCTGAGCTACTGTTTTTTAGGGACACTTAC‑TTTAAAGCACATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                                                <  1:529273/149‑1 (MQ=17)

                                                                     aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTGAAGCaa                                                                             >  2:1908518/1‑150 (MQ=255)

                                                                      ctctATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                            >  1:535263/1‑150 (MQ=255)

                                                                          aTCCAGCTGAGCTACGGATGCATCGGGATGGTTGCATTTGATG‑AGATAAATTGATACCGCTTATGAGGGCGTATGAAGTAAGTGATGGTGAATCCGGGAGTTTTAATCGCCTTGCGGCTCGCCCATCGGGAAGTTGATAAAGCAGCGtgg                                                                        >  2:479309/1‑148 (MQ=9)

                                                                            ccAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtc                                                                      >  2:1449749/1‑150 (MQ=255)

                                                                             cAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCAGGGAGGATTACTCGCCTTGCGGCTCGACCTTCGGGCCGTTGCTAAAGCGACGTTAtcc                                                                     >  2:1116318/1‑150 (MQ=255)

                                                                                cTGAGCTACGGATGCATCGGGAAAGTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAACGCCGTATCAAGTAAGAGATGGTGCATCAGGGAGGAATACTCGACTTGCGGCTCGCCCTAAGGGCCGTTCCTAAAGCAACGTTAtcctcc                                                                  >  1:268697/1‑150 (MQ=18)

                                                                                 tGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTccc                                                                 >  1:535466/1‑150 (MQ=255)

                                                                                   aGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTg                                                               >  2:1378871/1‑150 (MQ=255)

                                                                                   aGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTg                                                               >  2:732707/1‑150 (MQ=255)

                                                                                    gCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTgg                                                              >  2:577680/1‑150 (MQ=255)

                                                                                      tACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                            >  1:1019492/1‑150 (MQ=255)

                                                                                      tACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                            >  2:1701441/1‑150 (MQ=255)

                                                                                      tACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                            >  1:1898850/1‑150 (MQ=255)

                                                                                          gctGCACCGCGCAACTTAC‑TTAACTCCCGAGTTTTTCCTACCCCTACTAACGCCTTATCAAGTACGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTg                                                        <  1:1605805/148‑1 (MQ=14)

                                                                                            tGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTTCg                                                      >  1:969731/1‑150 (MQ=255)

                                                                                             gCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                     >  2:1471683/1‑150 (MQ=255)

                                                                                             gCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                     >  2:476868/1‑150 (MQ=255)

                                                                                             gCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                     >  2:849684/1‑150 (MQ=255)

                                                                                              cATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGcc                                                          <  2:236317/144‑1 (MQ=255)

                                                                                              cATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGcc                                                          >  1:236317/1‑144 (MQ=255)

                                                                                              cATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGt                                                    <  1:450658/150‑1 (MQ=255)

                                                                                              cATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGt                                                    >  2:865775/1‑150 (MQ=255)

                                                                                               ttCGGGAAAATTAA‑TTTATTGCTGTTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGtt                                                   <  2:1131255/149‑1 (MQ=17)

                                                                                                tCGGGAAACTTAC‑TTTACTGCAGTTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGtta                                                  <  2:1809263/150‑1 (MQ=255)

                                                                                                 cGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttat                                                 >  1:1865804/1‑150 (MQ=255)

                                                                                                 cGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttat                                                 >  1:83866/1‑150 (MQ=255)

                                                                                                  gggAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatt                                                >  2:58256/1‑150 (MQ=255)

                                                                                                  gggAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatt                                                >  1:587604/1‑150 (MQ=255)

                                                                                                   ggACACTTAC‑TTTACTGCAGATTTTTTGATACCGCAACAAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatta                                               <  2:1485748/150‑1 (MQ=255)

                                                                                                   ggAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatta                                               >  2:884163/1‑150 (MQ=255)

                                                                                                       acttac‑ttTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGc                                           >  1:1675058/1‑150 (MQ=255)

                                                                                                         ttac‑tttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCa                                         >  2:1501865/1‑150 (MQ=255)

                                                                                                           ac‑tttactTCCGCTATTATGATCCCGCAACAAAAGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAcc                                       <  2:794397/150‑1 (MQ=14)

                                                                                                           ac‑tttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAcc                                       >  2:8525/1‑150 (MQ=255)

                                                                                                               ttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACctct                                    >  2:939298/1‑150 (MQ=255)

                                                                                                                tactGCAGATTTTTTGATACCGATACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCCTTCGGGCCGTTGCAAAAGCAACTTTATCCTCCCTCGTGCCTGCGGATATTACCGCCACCTCtt                                   >  2:1529095/1‑150 (MQ=17)

                                                                                                                 tttGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTc                                  <  2:426471/148‑1 (MQ=255)

                                                                                                                  ctGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTcc                                 >  1:155171/1‑150 (MQ=255)

                                                                                                                    gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCaa                               >  2:39751/1‑149 (MQ=255)

                                                                                                                    gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                               >  1:1369509/1‑150 (MQ=255)

                                                                                                                    gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                               >  2:564734/1‑150 (MQ=255)

                                                                                                                    cccgATTTTTTCCTACCGCCCCTACCGCCGGAGCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                               <  1:391427/147‑1 (MQ=17)

                                                                                                                     cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                              >  1:1244671/1‑150 (MQ=255)

                                                                                                                     cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                              >  2:183441/1‑150 (MQ=255)

                                                                                                                     cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                              <  1:1043547/150‑1 (MQ=255)

                                                                                                                      aGATTTTTTGATACCGCTACTAAAGCCGTATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAATAAACGTTATCATCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATAc                             >  1:1203797/1‑150 (MQ=14)

                                                                                                                      aGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATAc                             <  2:658017/150‑1 (MQ=255)

                                                                                                                       gATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATAcc                            >  2:984919/1‑150 (MQ=255)

                                                                                                                         ttttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGATGATTACTCGCCTTGCGGCTCGCCATTCGGGCCGTTGCTAATGCTACGTTATCCTAACTGGTGCCTGCGCTTATTAACGACACCTCTTCCATAGCtt                          >  1:959037/1‑150 (MQ=17)

                                                                                                                             ttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATc                      <  1:970610/150‑1 (MQ=255)

                                                                                                                                aTACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGGTATCCTCCCTGGTGCCTGCGGGAATTACCGCCACCTCGTCAAAACCTTCCTCGcg                   >  1:1199350/1‑149 (MQ=17)

                                                                                                                                 tACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCCCCTCTTCCATACCTTCCTCGCTg                  >  2:1463926/1‑150 (MQ=255)

                                                                                                                                 tACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTg                  <  2:937068/150‑1 (MQ=255)

                                                                                                                                 tACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTg                  <  2:1368829/150‑1 (MQ=255)

                                                                                                                                 tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTcc                                 >  1:987851/1‑135 (MQ=16)

                                                                                                                                  aCCGCTACTAACGCCGTATTAAGTATTAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGa                 <  1:1639883/150‑1 (MQ=255)

                                                                                                                                   ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGTGGTTATTACCGCCACCTCTTACATACCTTCATCGCTGaa                >  1:682875/1‑150 (MQ=255)

                                                                                                                                   ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa                <  1:1000797/150‑1 (MQ=255)

                                                                                                                                   ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa                <  1:415144/150‑1 (MQ=255)

                                                                                                                                    cGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaaa               >  2:964166/1‑150 (MQ=255)

                                                                                                                                      ctactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCATCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGt             <  1:1745361/150‑1 (MQ=255)

                                                                                                                                          taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCTTGCCGTTGATTAAGCAACGTTATCCTCACTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt         >  1:1211562/1‑150 (MQ=17)

                                                                                                                                          taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt         >  2:1769761/1‑150 (MQ=255)

                                                                                                                                          taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt         <  2:535263/150‑1 (MQ=255)

                                                                                                                                           aaaGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGtt        <  2:948724/150‑2 (MQ=255)

                                                                                                                                           aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa        <  1:1131174/150‑1 (MQ=255)

                                                                                                                                           aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa        >  2:1089193/1‑150 (MQ=255)

                                                                                                                                           aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa        >  1:702614/1‑150 (MQ=255)

                                                                                                                                           aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa        >  2:153615/1‑150 (MQ=255)

                                                                                                                                           aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAcc                                             >  1:886936/1‑113 (MQ=19)

                                                                                                                                           aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAcc                                             <  2:886936/113‑1 (MQ=19)

                                                                                                                                            aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCt                           >  2:784350/1‑130 (MQ=255)

                                                                                                                                            aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCt                           <  1:784350/130‑1 (MQ=255)

                                                                                                                                            aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAg       >  2:200974/1‑150 (MQ=255)

                                                                                                                                             aGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGa      <  1:200974/150‑1 (MQ=255)

                                                                                                                                              gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa     >  1:1559239/1‑150 (MQ=255)

                                                                                                                                              gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa     >  1:895924/1‑150 (MQ=255)

                                                                                                                                              gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa     >  1:1603021/1‑150 (MQ=255)

                                                                                                                                              gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa     >  2:1116336/1‑150 (MQ=255)

                                                                                                                                               ccGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAat    <  2:1546791/150‑1 (MQ=255)

GCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAC‑TTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATAT  >  NC_000913/2818180‑2818473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`