BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,331	G→T	6.1%	intergenic (‑57/+142)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,331	0	G	T	6.1%	333.9 / 6.0	118	intergenic (‑57/+142)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (78/31); new base T (5/4); total (83/35)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

ATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCAT  >  NC_000913/2818183‑2818480

atatCAAGTAAGAGATGGTGCATCCGGGAGGATTACAACCTACGACCGCACGCATACTAGCCGAGTACTCAAACCAGCTGAGCTACGGATTCATCGGGAAACTTACATAACTCCAGATTTTTTGATACAGCTACTAAATCCGTATCAAGt                                                                                                                                                      >  2:1758432/1‑150 (MQ=255)

   tCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAag                                                                                                                                                   >  1:937068/1‑150 (MQ=255)

   tCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAag                                                                                                                                                   >  1:649610/1‑150 (MQ=255)

          agagATGGTGCATCCGGGAGGATTCGAACCAAAGACCCAACCCTTCCTAGCCGAGTACTCTATACAGATGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCAGTATCAAGTAAGAGATGGt                                                                                                                                            >  2:450658/1‑150 (MQ=17)

                          ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTa                                                                                                                            >  1:1368829/1‑150 (MQ=255)

                              gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCg                                                                                                                        <  1:1449567/150‑1 (MQ=255)

                                ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGATACTAACGCCGTATAAAGTAAGAGATGGTGAATCCGGGAGGATTACTCGcc                                                                                                                      >  2:970610/1‑150 (MQ=255)

                                  cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCtt                                                                                                                    >  1:655663/1‑150 (MQ=255)

                                     aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAAGGGATAGTTACTATTCTGCAGATTTTTTGATACCGATACTAAAGCGGTATCAAGTTAGAGATGGTGGATCAGGGAGGATTACTCGCCTTGCg                                                                                                                 >  2:674958/1‑150 (MQ=17)

                                      ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACGTACTTTACTGCAGATTTTTTGATGCCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                >  1:1136208/1‑150 (MQ=255)

                                        tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCTGGAAAATAACATTCCTGAAGATTAAATGATTCCGCTATTAAACCCATATAAAGTAACACTTGGTGAATCCTGGAGGATTACTCGCCAGGCGGCt                                                                                                              >  2:1786858/1‑150 (MQ=9)

                                          cGACCGCTATGTTCGTAGCCGTTTAATCTATCCAGCTTAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGTTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  2:1509843/150‑1 (MQ=17)

                                           gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTAGc                                                                                                           >  2:1036144/1‑150 (MQ=255)

                                               gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCtt                                                                                                       >  2:1000797/1‑150 (MQ=255)

                                                cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCATc                                                                                                      >  2:1745361/1‑150 (MQ=255)

                                                   ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                                                                   >  2:1097488/1‑150 (MQ=255)

                                                          tctcGAGTATTCTATCCAGCTGAGCTACCGATTTCTAGGGTAACTTATTTTATTGAAGATTTTTTGATACCGCTACTAAAGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                                                            <  2:1685105/147‑1 (MQ=17)

                                                               tgTACTCTATTCTGCTGAGCTACTGTTTTTTAGGGACACTTACTTTAAAGCACATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                                                       <  1:529273/149‑1 (MQ=17)

                                                                  aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTGAAGCaa                                                                                    >  2:1908518/1‑150 (MQ=255)

                                                                   ctctATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   >  1:535263/1‑150 (MQ=255)

                                                                   ctctATCCAGCTGAGCTACGGATGCATCGGGAAACGTACATTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATGCAGTAAGAGATGGTGCATCCGCGAGGAATACTCGCCTTGCCCCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   >  1:1663529/1‑150 (MQ=17)

                                                                         ccAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtc                                                                             >  2:1449749/1‑150 (MQ=255)

                                                                          cAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCAGGGAGGATTACTCGCCTTGCGGCTCGACCTTCGGGCCGTTGCTAAAGCGACGTTAtcc                                                                            >  2:1116318/1‑150 (MQ=255)

                                                                           aGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAtcct                                                                           >  2:871301/1‑150 (MQ=255)

                                                                             cTGAGCTACGGATGCATCGGGAAAGTTACTTTACTGCAGATTTTTTGATACCGCTACTAACGCCGTATCAAGTAAGAGATGGTGCATCAGGGAGGAATACTCGACTTGCGGCTCGCCCTAAGGGCCGTTCCTAAAGCAACGTTAtcctcc                                                                         >  1:268697/1‑150 (MQ=18)

                                                                              tGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTccc                                                                        >  1:535466/1‑150 (MQ=255)

                                                                                aGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTg                                                                      >  2:1378871/1‑150 (MQ=255)

                                                                                aGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTg                                                                      >  2:732707/1‑150 (MQ=255)

                                                                                 gCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTgg                                                                     >  2:577680/1‑150 (MQ=255)

                                                                                 gCTACGGATGCATCGGGAAACTTAATTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTgg                                                                     >  2:346202/1‑150 (MQ=255)

                                                                                   tACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                                   >  1:1019492/1‑150 (MQ=255)

                                                                                   tACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                                   >  2:1701441/1‑150 (MQ=255)

                                                                                   tACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTg                                                                   >  1:1898850/1‑150 (MQ=255)

                                                                                       gctGCACCGCGCAACTTACTTAACTCCCGAGTTTTTCCTACCCCTACTAACGCCTTATCAAGTACGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTg                                                               <  1:1605805/148‑1 (MQ=14)

                                                                                          gCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                            >  2:476868/1‑150 (MQ=255)

                                                                                          gCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCgg                                                            >  2:1471683/1‑150 (MQ=255)

                                                                                           cATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGcc                                                                 <  2:236317/144‑1 (MQ=255)

                                                                                           cATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGcc                                                                 >  1:236317/1‑144 (MQ=255)

                                                                                           cATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGt                                                           <  1:450658/150‑1 (MQ=255)

                                                                                           cATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGt                                                           >  2:865775/1‑150 (MQ=255)

                                                                                            ttCGGGAAAATTAATTTATTGCTGTTTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGtt                                                          <  2:1131255/149‑1 (MQ=17)

                                                                                             tCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGtta                                                         <  2:655663/150‑1 (MQ=255)

                                                                                              cGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttat                                                        >  1:1865804/1‑150 (MQ=255)

                                                                                               gggAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatt                                                       >  2:58256/1‑150 (MQ=255)

                                                                                                ggACACTTACTTTACTGCAGATTTTTTGATACCGCAACAAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatta                                                      <  2:1485748/150‑1 (MQ=255)

                                                                                                ggAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGttatta                                                      >  2:884163/1‑150 (MQ=255)

                                                                                                    acttacttTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGc                                                  >  1:1675058/1‑150 (MQ=255)

                                                                                                      ttactttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCa                                                >  2:1501865/1‑150 (MQ=255)

                                                                                                        actttactTCCGCTATTATGATCCCGCAACAAAAGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAcc                                              <  2:794397/150‑1 (MQ=14)

                                                                                                        actttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCAcc                                              >  2:8525/1‑150 (MQ=255)

                                                                                                           ttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACctct                                           >  2:939298/1‑150 (MQ=255)

                                                                                                            tactGCAGATTTTTTGATACCGATACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCCTTCGGGCCGTTGCAAAAGCAACTTTATCCTCCCTCGTGCCTGCGGATATTACCGCCACCTCtt                                          >  2:1529095/1‑150 (MQ=17)

                                                                                                             tttGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTc                                         <  2:426471/148‑1 (MQ=255)

                                                                                                             actGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTc                                         >  1:325055/1‑150 (MQ=255)

                                                                                                              ctGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTcc                                        >  1:155171/1‑150 (MQ=255)

                                                                                                                gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCaa                                      >  2:39751/1‑149 (MQ=255)

                                                                                                                gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                                      >  2:564734/1‑150 (MQ=255)

                                                                                                                gCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCGTCCAt                                      >  1:1354717/1‑150 (MQ=255)

                                                                                                                cccgATTTTTTCCTACCGCCCCTACCGCCGGAGCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCAt                                      <  1:391427/147‑1 (MQ=17)

                                                                                                                 cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                                     <  1:1043547/150‑1 (MQ=255)

                                                                                                                 cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                                     >  1:1244671/1‑150 (MQ=255)

                                                                                                                 cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATa                                     >  2:183441/1‑150 (MQ=255)

                                                                                                                   gATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATAcc                                   >  2:984919/1‑150 (MQ=255)

                                                                                                                     ttttttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGATGATTACTCGCCTTGCGGCTCGCCATTCGGGCCGTTGCTAATGCTACGTTATCCTAACTGGTGCCTGCGCTTATTAACGACACCTCTTCCATAGCtt                                 >  1:959037/1‑150 (MQ=17)

                                                                                                                         ttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATc                             <  1:970610/150‑1 (MQ=255)

                                                                                                                            aTACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGGTATCCTCCCTGGTGCCTGCGGGAATTACCGCCACCTCGTCAAAACCTTCCTCGcg                          >  1:1199350/1‑149 (MQ=17)

                                                                                                                             tACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCCCCTCTTCCATACCTTCCTCGCTg                         >  2:1463926/1‑150 (MQ=255)

                                                                                                                             tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTcc                                        >  1:987851/1‑135 (MQ=16)

                                                                                                                               ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGTGGTTATTACCGCCACCTCTTACATACCTTCATCGCTGaa                       >  1:682875/1‑150 (MQ=255)

                                                                                                                               ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa                       <  1:415144/150‑1 (MQ=255)

                                                                                                                               ccGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa                       <  1:1000797/150‑1 (MQ=255)

                                                                                                                                cGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaaa                      >  2:964166/1‑150 (MQ=255)

                                                                                                                                  ctactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCATCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGt                    <  1:1745361/150‑1 (MQ=255)

                                                                                                                                      taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCTTGCCGTTGATTAAGCAACGTTATCCTCACTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt                >  1:1211562/1‑150 (MQ=17)

                                                                                                                                      taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt                >  2:1769761/1‑150 (MQ=255)

                                                                                                                                      taAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGt                <  2:535263/150‑1 (MQ=255)

                                                                                                                                       aaaGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGtt               <  2:948724/150‑2 (MQ=255)

                                                                                                                                       aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa               >  1:702614/1‑150 (MQ=255)

                                                                                                                                       aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa               >  2:1089193/1‑150 (MQ=255)

                                                                                                                                       aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa               <  1:1131174/150‑1 (MQ=255)

                                                                                                                                       aaaGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTa               >  2:153615/1‑150 (MQ=255)

                                                                                                                                       aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAcc                                                    >  1:886936/1‑113 (MQ=19)

                                                                                                                                       aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTAcc                                                    <  2:886936/113‑1 (MQ=19)

                                                                                                                                        aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCt                                  >  2:784350/1‑130 (MQ=255)

                                                                                                                                        aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCt                                  <  1:784350/130‑1 (MQ=255)

                                                                                                                                        aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAg              >  2:200974/1‑150 (MQ=255)

                                                                                                                                         aGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGa             <  1:200974/150‑1 (MQ=255)

                                                                                                                                          gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa            >  1:1603021/1‑150 (MQ=255)

                                                                                                                                          gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa            >  1:1559239/1‑150 (MQ=255)

                                                                                                                                          gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa            >  1:895924/1‑150 (MQ=255)

                                                                                                                                          gCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGaa            >  2:1116336/1‑150 (MQ=255)

                                                                                                                                           ccGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAat           <  2:1546791/150‑1 (MQ=255)

                                                                                                                                             gTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAatat         <  1:940801/150‑1 (MQ=255)

                                                                                                                                              tATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACATCTTCCATACCTTCATCGCTGAAAGGACGTAGAATATg        >  2:264863/1‑150 (MQ=255)

                                                                                                                                               aTCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATgg       <  1:1758432/150‑1 (MQ=255)

                                                                                                                                                tCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGt      <  2:649610/150‑1 (MQ=255)

                                                                                                                                                 cAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGtt     >  1:938304/1‑149 (MQ=255)

                                                                                                                                                 cAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTTAAGCAACGTTCTCCGCCCTGGTGCCTGCGGTGATTACCGGCACCACTGCCGAACCTTCCTCGCGGAAAGTCGGTAGAATATGGTg     >  1:1237883/1‑150 (MQ=17)

                                                                                                                                                  aaGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATCCCTTCATCGCTGAAAGTACGTAGAATATGGTGc    >  1:986246/1‑150 (MQ=255)

                                                                                                                                                    gTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCAt  >  2:695584/1‑150 (MQ=255)

ATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCAT  >  NC_000913/2818183‑2818480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`