Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,822,678 G→T 6.9% intergenic (‑39/+30) recX ← / ← recA regulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,822,6780GT 6.9% 516.2 / 13.8 170intergenic (‑39/+30)recX/recAregulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Reads supporting (aligned to +/- strand):  ref base G (80/77);  new base T (5/8);  total (85/85)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

CGCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGG  >  NC_000913/2822529‑2822823
                                                                                                                                                     |                                                                                                                                                 
cgcgAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAg                                                                                                                                                   >  1:1667754/1‑150 (MQ=255)
cgcgAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAg                                                                                                                                                   >  1:1324818/1‑150 (MQ=255)
 gcgAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGa                                                                                                                                                  >  1:1063459/1‑150 (MQ=255)
   gAGTTTACGTCGCAGTTCATGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATg                                                                                                                                                <  2:1534818/150‑1 (MQ=255)
     gTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCATCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAACAAGCAAAAGGGCCGCAGATGCg                                                                                                                                              >  1:168648/1‑150 (MQ=255)
         aCGTCGCAGTTCTTACTCACTGTGATCGCGCACCGCCAGAATGCGTACCTCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAATGGCCGCAGATGCGAccc                                                                                                                                          <  1:1166144/150‑1 (MQ=255)
         aCGTCGCAGTTCTTACTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGAccc                                                                                                                                          <  1:1927733/150‑1 (MQ=255)
          cGTCGCAGTTCTTGCTCACTTTGAGCGCGCACCGCCAGAATGCGAACCGCACGATCCAACAGGCGAGCATATGCCGTGCGACTGGATGTTGATTCTGTCATGGAATATCCTTACAACTTAAAAAAGCAAAAGGGTCACATCTGCGGCCCt                                                                                                                                         <  2:1871469/150‑1 (MQ=255)
                aGTTCTTGCTCTCTGTGCTCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACATCTTAAAAAAGCAAAAGGGCCGCATATGCGACCCTTGTGTa                                                                                                                                   <  1:70660/150‑1 (MQ=255)
                aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgct                                                                                                                                   <  1:1306253/150‑3 (MQ=255)
                aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa                                                                                                                                   <  2:859111/150‑1 (MQ=255)
                aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa                                                                                                                                   <  2:669765/150‑1 (MQ=255)
                 gTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTAt                                                                                                                                  >  1:1157736/1‑150 (MQ=255)
                 gTTCATGCTCACTGTGATCGCGCACCTCCAGAATGCGTACCGCACGATCCAACAGGCTAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACCACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgctt                                                                                                                                  <  1:1203004/150‑4 (MQ=255)
                   tCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCa                                                                                                                                <  1:853228/150‑1 (MQ=255)
                    cTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGTTGCGACCCTTGTGTATCaa                                                                                                                               >  2:1231044/1‑150 (MQ=255)
                    cTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCaa                                                                                                                               <  1:1429064/150‑1 (MQ=255)
                     ttGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCata                                                                                                                              >  1:1248572/1‑148 (MQ=255)
                     ttGCTCACTGTGATCGCGCACCGCCAGAATGCCTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgctttttt                                                                                                                              <  2:1092184/150‑8 (MQ=255)
                      tGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGATGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAAc                                                                                                                             >  2:1173188/1‑150 (MQ=255)
                      tGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAAc                                                                                                                             <  1:382471/150‑1 (MQ=255)
                              gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGAt                                                                                                                     >  2:156532/1‑150 (MQ=255)
                              gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                     <  2:46270/150‑1 (MQ=255)
                              gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                     >  2:1723562/1‑150 (MQ=255)
                              gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                     >  1:907571/1‑150 (MQ=255)
                                 aTCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCAAGTGCCGGGCGACGGGAGGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTaa                                                                                                                  >  2:2013559/1‑150 (MQ=255)
                                   cgcgCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTaaaa                                                                                                                >  2:827214/1‑150 (MQ=255)
                                       cACCGCCAGAATGCGTACCGCACGATCCCACAGGCGAGCATATGCCGGGCGACGGGATGTTGAGTCTGTCATGGCATATCCTGACAACTTAACAAAGCAAAAGGGCCGCAGCTGCGCCCCTTGTGTATCAAACAAGACGAGTAAAAATcg                                                                                                            >  1:285191/1‑149 (MQ=255)
                                       cACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCCGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCt                                                                                                            >  2:1225374/1‑150 (MQ=255)
                                         ccGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc                                                                                                          <  2:1343742/150‑1 (MQ=255)
                                             cAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTa                                                                                                      >  2:664320/1‑150 (MQ=255)
                                                aaTGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGtt                                                                                                   <  1:590170/150‑1 (MQ=255)
                                                aaTGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGtt                                                                                                   <  1:1983544/150‑1 (MQ=255)
                                                    cGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGAAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTg                                                                                               >  2:69829/1‑150 (MQ=255)
                                                     gTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCtac                                                                                              >  2:1670674/1‑148 (MQ=255)
                                                     gTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGc                                                                                              >  1:1145743/1‑150 (MQ=255)
                                                     gTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGc                                                                                              <  2:1560140/150‑1 (MQ=255)
                                                     gTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGc                                                                                              <  2:1109877/150‑1 (MQ=255)
                                                              gATCCAACAGCCGAGCATATGCCGGGCGACGGGATGTTTATTCTGTCATGGCATATGCTTACAAATTAAAAAAGCAAAATAGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAAAATTCGGTAGTTTATGCTACGCCtac                                                                                     >  2:1438683/1‑148 (MQ=255)
                                                                tCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGc                                                                                   <  1:1399035/150‑1 (MQ=255)
                                                                  cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                 >  1:146871/1‑150 (MQ=255)
                                                                  cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTTAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                 >  1:1020734/1‑150 (MQ=255)
                                                                  cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                 >  1:1464661/1‑150 (MQ=255)
                                                                  cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTATATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCtt                                                                                 >  1:1884915/1‑149 (MQ=255)
                                                                   aaCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTat                                                                                <  1:1853247/150‑1 (MQ=255)
                                                                   aaCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTat                                                                                <  1:1197425/150‑1 (MQ=255)
                                                                    aaacgCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCACAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                               <  2:527017/146‑1 (MQ=255)
                                                                    aCAGGCGAGCATATGCCGGGCGACGGGATGTTTATTCTGTCATGGCATATCCTTACAACTAAAAAAAGCAAAAGGGCCGCATATGCGACCCTTGTGTATCAAACAAGCCGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                               <  2:1061679/150‑1 (MQ=255)
                                                                    aCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                               <  2:884093/150‑1 (MQ=255)
                                                                    aCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                               >  2:275931/1‑150 (MQ=255)
                                                                     cAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatca                                                                              <  1:1118455/150‑1 (MQ=255)
                                                                       ggCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatcatc                                                                            >  1:1414746/1‑150 (MQ=255)
                                                                         cGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTATTCTACGCCTTCGCTATCATCTa                                                                          >  2:1785116/1‑150 (MQ=255)
                                                                          gatcATATGCCGGTCGACGGGATGTTGATTCTGTCATGGCTTATCCTTACAACTTAAATAAGCAAAACGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTAc                                                                         <  1:327062/147‑1 (MQ=255)
                                                                           aGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACa                                                                        >  2:1082101/1‑150 (MQ=255)
                                                                             cATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAATCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACaga                                                                      <  1:1783609/150‑1 (MQ=255)
                                                                              atatGACGGGCGACGGGAGGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACagag                                                                     <  1:1009665/150‑1 (MQ=255)
                                                                                  gCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAAt                                                                 >  2:1810403/1‑150 (MQ=255)
                                                                                    cGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATcc                                                               >  1:667029/1‑150 (MQ=255)
                                                                                      ggCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGTCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCgg                                                             <  2:31510/150‑1 (MQ=255)
                                                                                        cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCg                                                           >  1:545778/1‑150 (MQ=255)
                                                                                        cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCg                                                           >  1:1993783/1‑150 (MQ=255)
                                                                                        cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCg                                                           >  2:711490/1‑150 (MQ=255)
                                                                                          aCGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTCGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGtt                                                         <  2:629785/150‑1 (MQ=255)
                                                                                           cGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                        <  2:1473268/150‑1 (MQ=255)
                                                                                           cGGGATGTTGATTCTGTCATGGCATATCCTTAAAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                        >  1:1105623/1‑150 (MQ=255)
                                                                                           agggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                        <  1:1890022/149‑1 (MQ=255)
                                                                                             ggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAg                                                      >  1:1089902/1‑150 (MQ=255)
                                                                                                 gTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCg                                                  >  1:120962/1‑150 (MQ=255)
                                                                                                   tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCTGCGTTGAGTTCggg                                                >  2:1394298/1‑150 (MQ=255)
                                                                                                   tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCggg                                                >  2:1552021/1‑150 (MQ=255)
                                                                                                     aTTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt                                              >  2:1968905/1‑150 (MQ=255)
                                                                                                          gTCATGGCATATCCATACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGAATAAAAATCTACGTTAGTTACTGCTACGCCTTCGCTTTCATCTACAGAGAAATCCGGCGTTGAGATCGGGTTGCTca                                         >  1:469539/1‑150 (MQ=255)
                                                                                                            cATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                       <  1:195011/150‑1 (MQ=255)
                                                                                                            cATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                       >  2:619946/1‑150 (MQ=255)
                                                                                                            cATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                       >  2:1416728/1‑150 (MQ=255)
                                                                                                               ggCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCagcagc                                    <  1:1443793/150‑1 (MQ=255)
                                                                                                                gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACCGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAgcagca                                   <  2:920813/150‑1 (MQ=255)
                                                                                                                gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAgcagca                                   >  1:33081/1‑150 (MQ=255)
                                                                                                                     tCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCa                              <  2:390347/150‑1 (MQ=255)
                                                                                                                       cTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACg                            >  2:943822/1‑150 (MQ=255)
                                                                                                                           cAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACt                        >  1:1084569/1‑150 (MQ=255)
                                                                                                                              cTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAACTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttta                     >  2:1312236/1‑149 (MQ=255)
                                                                                                                              cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttta                     >  1:938199/1‑149 (MQ=255)
                                                                                                                              cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                     <  2:476369/150‑1 (MQ=255)
                                                                                                                              cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                     <  2:885619/150‑1 (MQ=255)
                                                                                                                                   aaaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCCGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTtctc                >  2:850861/1‑150 (MQ=255)
                                                                                                                                    aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCg               <  2:813722/150‑1 (MQ=255)
                                                                                                                                     atacacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGa              >  2:1917521/7‑150 (MQ=255)
                                                                                                                                      tacacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAt             >  1:1709908/6‑150 (MQ=255)
                                                                                                                                       acacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCGACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtg            >  1:1754438/5‑149 (MQ=255)
                                                                                                                                        cacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGAGTTAAAATCATCGTTAGTTTCGGCTACGCCTTCGCTATCTTATACAGAGAAATACGCCGATTCGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtct           >  1:1757526/4‑150 (MQ=255)
                                                                                                                                          aaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATctct         <  1:133609/150‑1 (MQ=255)
                                                                                                                                              ggCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcg     <  1:1648342/150‑1 (MQ=255)
                                                                                                                                              ggCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcg     >  1:1561210/1‑150 (MQ=255)
                                                                                                                                               gCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcgc    <  1:550307/150‑1 (MQ=255)
                                                                                                                                               gCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcgc    >  2:1252393/1‑150 (MQ=255)
                                                                                                                                               cccccAGAGGCGACCCTTGTTTATAAAACAAGACTATAAAAAATCTTCTTTAGTTTCTGCACCGCCTCCGCTAACATCACCCCACAAACCCGGCGTTTCGTGCGGGTTGCCCAGCACAAACTCACGTACTTTCTTCTCGATCTCTTgcgc    <  2:1716155/146‑1 (MQ=255)
                                                                                                                                                 cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg  <  2:1464661/150‑1 (MQ=255)
                                                                                                                                                 cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg  <  2:893991/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGG  >  NC_000913/2822529‑2822823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: