Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 256,449 T→C 7.8% intergenic (+14/‑78) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913256,4490TC 7.8% 519.2 / 23.5 179intergenic (+14/‑78)gpt/frsAxanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base T (77/86);  new base C (6/8);  total (84/95)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

Only 100 of 180 total aligned reads displayed.

TTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTC  >  NC_000913/256302‑256598
                                                                                                                                                   |                                                                                                                                                     
ttGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTATTCAACGCCTgg                                                                                                                                                     >  2:351190/1‑150 (MQ=255)
 tGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTTGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGc                                                                                                                                                    <  1:385572/150‑1 (MQ=255)
    cACCATCTTCGCAAAACCGGATGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCACAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACt                                                                                                                                                 >  2:438139/1‑150 (MQ=255)
          cTTCGCAAAACCGGCTTGTCGGCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                                                                           <  2:509489/150‑1 (MQ=255)
          cTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                                                                           <  1:866413/150‑1 (MQ=255)
             cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgt                                                                                                                                        >  2:1764590/1‑150 (MQ=255)
             cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgt                                                                                                                                        <  2:1045816/150‑1 (MQ=255)
              gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGTGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCggt                                                                                                                                       >  2:1689162/1‑148 (MQ=255)
              gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCctt                                                                                                                                       >  1:1085128/1‑150 (MQ=255)
                aaaaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttgt                                                                                                                                     >  1:1829792/1‑150 (MQ=255)
                aaaaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttga                                                                                                                                     <  1:1429763/150‑2 (MQ=255)
                aaaaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCACTGCCGGGCgttgt                                                                                                                                     >  2:469972/1‑150 (MQ=255)
                 aaaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgaa                                                                                                                                    <  2:496673/150‑3 (MQ=255)
                  aaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTc                                                                                                                                   >  1:1029029/1‑150 (MQ=255)
                   aCCTGCTGGTCGTCCGCTGGTTGATGACTATTTTGTTGATATCCCGCAAGATACCTGGAATGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCt                                                                                                                                  <  2:178524/150‑1 (MQ=255)
                   aCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttgaaaa                                                                                                                                  <  2:922155/150‑5 (MQ=255)
                    ccGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt                                                                                                                                 <  1:1582185/150‑1 (MQ=255)
                    ccGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATATCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGTGCGTTGTTCtt                                                                                                                                 >  2:231994/1‑150 (MQ=255)
                      gggctgtcgtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtttt                                                                                                                               <  2:652839/145‑1 (MQ=255)
                      ggCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGAAACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCGGGCTCTGCCGGGCGTTGTTCttgt                                                                                                                               >  2:142757/1‑148 (MQ=255)
                        cTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTa                                                                                                                             <  2:950883/150‑1 (MQ=255)
                         tGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa                                                                                                                            <  1:664597/150‑1 (MQ=255)
                          ggTCGTCCGCTGGTTGATGACTTTGTTGTTGATAGCCCGCAAGTTACCTGGATTGTACAGCCGTGGGATATGGGCGTCGTATTAGTCCAGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTatc                                                                                                                           <  2:2013477/150‑3 (MQ=255)
                          ggTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                                                                           <  1:603221/150‑1 (MQ=255)
                          ggTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                                                                           <  1:1656510/150‑1 (MQ=255)
                           gtcgtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACt                                                                                                                          >  1:1517442/1‑150 (MQ=255)
                             cgtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTATTTTTAACtta                                                                                                                        >  2:1712367/1‑149 (MQ=255)
                              gtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCTATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                                                                       >  1:247645/1‑150 (MQ=255)
                              gtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCa                                                                                                                       >  1:769368/1‑150 (MQ=255)
                                  gCTGGTTGATGACTTTGTTGTTGATATCCCGCAAGATACCTGGATTGACCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAAGCTCCGGTCGCTAATCTTTTCAACGCCTGGCCCTGCCGGGCGTTGGTCTTTGTAACTTCAGGCg                                                                                                                   >  2:119639/1‑150 (MQ=255)
                                  gCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCg                                                                                                                   >  2:477672/1‑150 (MQ=255)
                                    tGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCggg                                                                                                                 <  1:1644602/150‑1 (MQ=255)
                                     gtttGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGGGGGATATGGGCTGCGTTTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTATCTTCAGGCGGGt                                                                                                                <  2:1393121/148‑1 (MQ=255)
                                     ggTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGt                                                                                                                <  1:568315/150‑1 (MQ=255)
                                         gatgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACa                                                                                                            <  1:1850694/150‑1 (MQ=255)
                                         gatgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACa                                                                                                            <  1:1858384/150‑1 (MQ=255)
                                           tgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAAt                                                                                                          >  1:461550/1‑150 (MQ=255)
                                           tgaCTATGTTGTGGCTATCCCGCAAGATACCGGGATTGAACTGCCGTTGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAAAGCCTGGCACTGCCGTGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAAt                                                                                                          <  1:92119/150‑1 (MQ=255)
                                                ttgttgtTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCTGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGttt                                                                                                     <  1:7176/149‑1 (MQ=255)
                                                aTGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTTGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGttt                                                                                                     <  1:1812680/150‑1 (MQ=255)
                                                  gttgttGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTcc                                                                                                   <  2:762974/150‑1 (MQ=255)
                                                              ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCACCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAttat                                                                                       >  2:624389/1‑148 (MQ=255)
                                                               cGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                                                                                      >  1:1567942/1‑150 (MQ=255)
                                                               cGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                                                                                      <  1:2014327/150‑1 (MQ=255)
                                                               cGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGGCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTg                                                                                      <  1:358305/150‑1 (MQ=255)
                                                                gctaGATACCTGGATTGAAGAGCCGTGGGATATGGCCGTCGTATTCGTCACGCCAATCTCCGGTCTCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGTGTTACAATAGTTTCCAGTAAGTATTCttg                                                                                     <  2:277350/147‑3 (MQ=255)
                                                                   aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAgg                                                                                  <  2:1943198/150‑1 (MQ=255)
                                                                   aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAgg                                                                                  >  2:1226194/1‑150 (MQ=255)
                                                                   aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAgg                                                                                  <  2:530002/150‑1 (MQ=255)
                                                                      tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTg                                                                               >  1:814994/1‑150 (MQ=255)
                                                                           ggATTGAACAGCCGGGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTGCACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATcc                                                                          <  2:1232762/150‑1 (MQ=255)
                                                                           ggAGTGAACAGCCGTGGGATATGGGCGGCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCATTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCTGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATcc                                                                          <  2:1598137/150‑1 (MQ=255)
                                                                            gATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCa                                                                         <  1:1615455/150‑1 (MQ=255)
                                                                               tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGa                                                                      >  2:1105628/1‑150 (MQ=255)
                                                                                gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGGCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGac                                                                     >  2:364690/1‑150 (MQ=255)
                                                                                gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTAAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGac                                                                     >  2:1034036/1‑150 (MQ=255)
                                                                                gAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGTATCTTTTCAACGCCTGGCACTGCCGGGCGTTGGTCTTTTTAAATTACGTGGGGTTACTATTGTTTACAGTAAGTATTCTGGGGGCTTCGTTCATGac                                                                     >  2:1777277/1‑150 (MQ=255)
                                                                                     gCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTATGGAGGCTGCATCCATGACACAgg                                                                >  2:712953/1‑150 (MQ=255)
                                                                                      ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGc                                                               >  2:458399/1‑150 (MQ=255)
                                                                                       cGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTGTTTTTAACTTCAGGCGGGTTACAATTGTTTCCAGTAAGTATTATGGTGGCTGCATCCATGACACAGGCa                                                              >  1:1659758/1‑150 (MQ=255)
                                                                                        gTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaa                                                             <  2:769368/150‑1 (MQ=255)
                                                                                         ggggATAGGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaaa                                                            <  2:1056072/149‑1 (MQ=255)
                                                                                               atGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAg                                                      <  1:1546350/150‑1 (MQ=255)
                                                                                                  ggCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTTACGTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATGCATGCCACAGGCAAACCTGAGCGa                                                   >  2:850281/1‑150 (MQ=255)
                                                                                                    cgtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa                                                 <  1:1153459/150‑1 (MQ=255)
                                                                                                       cgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTATCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAccc                                              <  1:970806/150‑1 (MQ=255)
                                                                                                         tATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTg                                            >  2:1167039/1‑150 (MQ=255)
                                                                                                            tCGTCCCGCCAATCTCCGGGCGCTAATCTTTTCAACGCCGGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACGGGCAAACCTGAGCGAAACCCTGTTc                                         <  1:1214294/150‑1 (MQ=255)
                                                                                                              gTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCaa                                       <  1:1286428/150‑1 (MQ=255)
                                                                                                                cccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAAc                                     >  1:1456115/1‑150 (MQ=255)
                                                                                                                cccGCCAAGCTGGGGTCCGTATTGTTTTAAACGCGTGGCCCTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTTCAATTGTTCCATTATGTATTCGGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAATCCCTGTTCAAAcc                                    <  1:1422681/150‑1 (MQ=255)
                                                                                                                 ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGCCAGAGGCAAACCGGAGCGAAACCCTGTTCAAAcc                                    >  1:57988/1‑150 (MQ=255)
                                                                                                                          tcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTaa                           <  2:1050436/150‑1 (MQ=255)
                                                                                                                          tcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGATCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTaa                           >  1:369834/1‑150 (MQ=255)
                                                                                                                           ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTaaa                          <  1:1723597/150‑1 (MQ=255)
                                                                                                                            ctgtagCTAATCTTTTCAACTCCTGGCACTGCCGGGAGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAAc                         <  1:1333976/145‑1 (MQ=255)
                                                                                                                            cGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAAc                         <  1:477672/150‑1 (MQ=255)
                                                                                                                              gTCGCTAATCTTTTCAACGCCTTGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACAt                       <  1:67380/150‑1 (MQ=255)
                                                                                                                              gTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACAt                       <  1:846175/150‑1 (MQ=255)
                                                                                                                                cGCTAATCTTTTCAACTCCTGGCACTGCCGGGAGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATcc                     <  1:119639/150‑1 (MQ=255)
                                                                                                                                cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATcc                     <  2:554458/150‑1 (MQ=255)
                                                                                                                                  cTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTg                   >  1:1395988/1‑150 (MQ=255)
                                                                                                                                    aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGaa                 <  1:231419/150‑1 (MQ=255)
                                                                                                                                    aaTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGaa                 <  1:609492/150‑1 (MQ=255)
                                                                                                                                     aaaagaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGCCACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTGTAAACATCCTGaaa                >  1:1001162/8‑150 (MQ=255)
                                                                                                                                       gttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAAcc              <  2:1517442/149‑1 (MQ=255)
                                                                                                                                       cTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAAcc              >  1:944331/1‑150 (MQ=255)
                                                                                                                                       aagaacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAAcc              >  2:422961/6‑150 (MQ=255)
                                                                                                                                        ttttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCGGCTTTAAACATCCTGAAACCt             >  2:1812245/1‑150 (MQ=255)
                                                                                                                                         tttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGATTTAAACATCCTGAAACCTc            >  2:1803969/1‑150 (MQ=255)
                                                                                                                                           acAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGa          >  2:1610483/2‑150 (MQ=255)
                                                                                                                                            cAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGAc         <  1:438139/150‑1 (MQ=255)
                                                                                                                                             aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGAATTCTGGCGGCTGCATCCATGCCACAGGCAAACCTGAGCGAACCCCTGTTAAAACCCCGCTTTAAACATCCTGCAACCTCGAcc        >  1:456506/1‑149 (MQ=255)
                                                                                                                                              aCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGc       >  2:1815864/1‑150 (MQ=255)
                                                                                                                                              aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCGTTAAACATCCTGAAACCTCGACGc       >  2:1942715/1‑150 (MQ=255)
                                                                                                                                               cGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCt      <  2:1081201/150‑1 (MQ=255)
                                                                                                                                                gCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTa     <  1:494040/150‑1 (MQ=255)
                                                                                                                                                 ccTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAACCCCCGCTTTAAACATCCTGAAACCTCGACGCTAg    >  2:574619/1‑150 (MQ=255)
                                                                                                                                                 ccTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAg    <  1:1910871/150‑1 (MQ=255)
                                                                                                                                                  cTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGt   >  1:1426686/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTC  >  NC_000913/256302‑256598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: