Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,822,679 A→C 8.6% intergenic (‑40/+29) recX ← / ← recA regulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,822,6790AC 8.6% 484.6 / 21.4 169intergenic (‑40/+29)recX/recAregulatory protein for RecA/DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Reads supporting (aligned to +/- strand):  ref base A (73/79);  new base C (9/6);  total (84/85)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

GCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCC  >  NC_000913/2822530‑2822828
                                                                                                                                                     |                                                                                                                                                     
gcgAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGa                                                                                                                                                       >  1:1063459/1‑150 (MQ=255)
  gAGTTTACGTCGCAGTTCATGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATg                                                                                                                                                     <  2:1534818/150‑1 (MQ=255)
      ttACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGAc                                                                                                                                                 <  2:1208188/150‑1 (MQ=255)
       tACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCATCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAACGCAAAAGGGCCGCAGATGCGccc                                                                                                                                                >  2:280834/1‑150 (MQ=255)
        aCGTCGCAGTTCTTACTCACTGTGATCGCGCACCGCCAGAATGCGTACCTCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAATGGCCGCAGATGCGAccc                                                                                                                                               <  1:1166144/150‑1 (MQ=255)
         cGTCGCAGTTCTTGCTCACTTTGAGCGCGCACCGCCAGAATGCGAACCGCACGATCCAACAGGCGAGCATATGCCGTGCGACTGGATGTTGATTCTGTCATGGAATATCCTTACAACTTAAAAAAGCAAAAGGGTCACATCTGCGGCCCt                                                                                                                                              <  2:1871469/150‑1 (MQ=255)
           tCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtg                                                                                                                                            >  1:178567/1‑150 (MQ=255)
             gCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTtgtg                                                                                                                                          <  1:1858503/150‑1 (MQ=255)
               aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgct                                                                                                                                        <  1:1306253/150‑3 (MQ=255)
               aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa                                                                                                                                        <  2:859111/150‑1 (MQ=255)
               aGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTa                                                                                                                                        <  2:669765/150‑1 (MQ=255)
                gTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTAt                                                                                                                                       >  1:1157736/1‑150 (MQ=255)
                gTTCATGCTCACTGTGATCGCGCACCTCCAGAATGCGTACCGCACGATCCAACAGGCTAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACCACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgctt                                                                                                                                       <  1:1203004/150‑4 (MQ=255)
                   cTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgcttttt                                                                                                                                    <  1:1660463/150‑7 (MQ=255)
                   cTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGTTGCGACCCTTGTGTATCaa                                                                                                                                    >  2:1231044/1‑150 (MQ=255)
                   cTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCaa                                                                                                                                    <  1:1429064/150‑1 (MQ=255)
                    ttGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCata                                                                                                                                   >  1:1248572/1‑148 (MQ=255)
                    ttGCTCACTGTGATCGCGCACCGCCAGAATGCCTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGTCGCATCTGCGGCCCTtttgctttttt                                                                                                                                   <  2:1092184/150‑8 (MQ=255)
                       cTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACaa                                                                                                                                <  1:401121/150‑1 (MQ=255)
                           cTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACg                                                                                                                            >  1:1716155/1‑150 (MQ=255)
                             gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGAt                                                                                                                          >  2:156532/1‑150 (MQ=255)
                             gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                          <  2:46270/150‑1 (MQ=255)
                             gtgATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGAt                                                                                                                          >  1:907571/1‑150 (MQ=255)
                                aTCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCAAGTGCCGGGCGACGGGAGGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTaa                                                                                                                       >  2:2013559/1‑150 (MQ=255)
                                  cgcgCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTaaaa                                                                                                                     >  2:827214/1‑150 (MQ=255)
                                      cACCGCCAGAATGCGTACCGCACGATCCCACAGGCGAGCATATGCCGGGCGACGGGATGTTGAGTCTGTCATGGCATATCCTGACAACTTAACAAAGCAAAAGGGCCGCAGCTGCGCCCCTTGTGTATCAAACAAGACGAGTAAAAATcg                                                                                                                 >  1:285191/1‑149 (MQ=255)
                                      cACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCCGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCt                                                                                                                 >  2:1225374/1‑150 (MQ=255)
                                        ccGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc                                                                                                               <  2:1343742/150‑1 (MQ=255)
                                        ccGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTc                                                                                                               >  1:1140712/1‑150 (MQ=255)
                                         cGCCAGAATGCGTACCGCCCGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTAAAAAAACAAAAAGGGCCGACGGGGCGACCCTGGGGTTACAAACATGACGATTAAAAATCTTCg                                                                                                              >  2:1730036/1‑150 (MQ=255)
                                            cAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTa                                                                                                           >  2:664320/1‑150 (MQ=255)
                                               aaTGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGtt                                                                                                        <  1:590170/150‑1 (MQ=255)
                                                   cGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGAAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTg                                                                                                    >  2:69829/1‑150 (MQ=255)
                                                    gTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCtac                                                                                                   >  2:1670674/1‑148 (MQ=255)
                                                     tACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCt                                                                                                  >  2:633441/1‑150 (MQ=255)
                                                             gATCCAACAGCCGAGCATATGCCGGGCGACGGGATGTTTATTCTGTCATGGCATATGCTTACAAATTAAAAAAGCAAAATAGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAAAATTCGGTAGTTTATGCTACGCCtac                                                                                          >  2:1438683/1‑148 (MQ=255)
                                                               tCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGc                                                                                        <  1:1399035/150‑1 (MQ=255)
                                                               tCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGc                                                                                        <  1:194897/150‑1 (MQ=255)
                                                               tCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGc                                                                                        <  1:722247/150‑1 (MQ=255)
                                                                 cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                      >  1:146871/1‑150 (MQ=255)
                                                                 cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTTAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                      >  1:1020734/1‑150 (MQ=255)
                                                                 cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTa                                                                                      <  2:1563393/150‑1 (MQ=255)
                                                                 cAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTATATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCtt                                                                                      >  1:1884915/1‑149 (MQ=255)
                                                                  aaCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTat                                                                                     <  1:1197425/150‑1 (MQ=255)
                                                                   aaacgCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCACAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                                    <  2:527017/146‑1 (MQ=255)
                                                                   aCAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatc                                                                                    <  2:884093/150‑1 (MQ=255)
                                                                    cAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatca                                                                                   <  1:1118455/150‑1 (MQ=255)
                                                                      ggCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatcatc                                                                                 >  1:1414746/1‑150 (MQ=255)
                                                                       gctaGCATATGCCGGGCGACGGGATGTTGTTTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAGGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGTCGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCt                                                                                <  1:1902635/147‑1 (MQ=255)
                                                                        cGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTATTCTACGCCTTCGCTATCATCTa                                                                               >  2:1785116/1‑150 (MQ=255)
                                                                        cGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTa                                                                               >  1:893991/1‑150 (MQ=255)
                                                                         gatcATATGCCGGTCGACGGGATGTTGATTCTGTCATGGCTTATCCTTACAACTTAAATAAGCAAAACGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTAc                                                                              <  1:327062/147‑1 (MQ=255)
                                                                          aGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACa                                                                             >  2:1082101/1‑150 (MQ=255)
                                                                           gCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTatcatc                                                                                 >  2:1430491/1‑145 (MQ=255)
                                                                             atatGACGGGCGACGGGAGGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACagag                                                                          <  1:1009665/150‑1 (MQ=255)
                                                                                 gCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAAt                                                                      >  2:1810403/1‑150 (MQ=255)
                                                                                   cGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATcc                                                                    >  1:667029/1‑150 (MQ=255)
                                                                                     ggCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGTCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCgg                                                                  <  2:31510/150‑1 (MQ=255)
                                                                                       cGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCg                                                                >  2:711490/1‑150 (MQ=255)
                                                                                          cGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                             <  2:1063459/150‑1 (MQ=255)
                                                                                          cGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                             <  2:1305613/150‑1 (MQ=255)
                                                                                          cGGGATGTTGATTCTGTCATGGCATATCCTTAAAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTg                                                             >  1:1105623/1‑150 (MQ=255)
                                                                                            ggATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAg                                                           >  1:1089902/1‑150 (MQ=255)
                                                                                                gTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCg                                                       >  1:120962/1‑150 (MQ=255)
                                                                                                  tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCggg                                                     <  1:1764690/150‑1 (MQ=255)
                                                                                                  tGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCggg                                                     >  2:1552021/1‑150 (MQ=255)
                                                                                                   gATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGCTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGt                                                    <  1:198397/150‑1 (MQ=255)
                                                                                                    aTTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGtt                                                   >  2:1968905/1‑150 (MQ=255)
                                                                                                      tCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGc                                                 <  2:1533713/150‑1 (MQ=255)
                                                                                                         gTCATGGCATATCCATACAACTTAAAAAAGCAAAAGGGCCGCAGCTGCGACCCTTGTGTATCAAACAAGACGAATAAAAATCTACGTTAGTTACTGCTACGCCTTCGCTTTCATCTACAGAGAAATCCGGCGTTGAGATCGGGTTGCTca                                              >  1:469539/1‑150 (MQ=255)
                                                                                                           cATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                            <  1:195011/150‑1 (MQ=255)
                                                                                                           cATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTcagc                                            >  2:619946/1‑150 (MQ=255)
                                                                                                             tGGCATATCCTTACAACGTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCGATCATCTACAGAGAAAGCCGGCGGTGAGTTCGGGTTGCTcagcag                                          >  1:339862/1‑150 (MQ=255)
                                                                                                              ggCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCagcagc                                         <  1:1443793/150‑1 (MQ=255)
                                                                                                               gCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACCGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAgcagca                                        <  2:920813/150‑1 (MQ=255)
                                                                                                                    tCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCa                                   <  2:390347/150‑1 (MQ=255)
                                                                                                                     ccTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCAc                                  >  1:1444213/1‑150 (MQ=255)
                                                                                                                      cTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACg                                 >  2:943822/1‑150 (MQ=255)
                                                                                                                      cTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACg                                 >  1:278169/1‑150 (MQ=255)
                                                                                                                       ttACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGt                                <  1:581247/150‑1 (MQ=255)
                                                                                                                          cAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACt                             >  1:1084569/1‑150 (MQ=255)
                                                                                                                             cTTAAAAAAGCAAAAGGGCCGCAGATGCGCCCCTTGTGTATCAAACAAGACGATTAAAACTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttta                          >  2:1312236/1‑149 (MQ=255)
                                                                                                                             cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACttta                          >  1:938199/1‑149 (MQ=255)
                                                                                                                             cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                          <  2:885619/150‑1 (MQ=255)
                                                                                                                             cTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTttc                          <  2:476369/150‑1 (MQ=255)
                                                                                                                                   aaaGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCg                    <  2:813722/150‑1 (MQ=255)
                                                                                                                                    atacacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGa                   >  2:1917521/7‑150 (MQ=255)
                                                                                                                                     tacacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCCCGTACTTTCTTCTCGAt                  >  2:858519/6‑150 (MQ=255)
                                                                                                                                      acacaaGGGTCGCATCTGCGGCCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCGACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGAtg                 >  1:1754438/5‑149 (MQ=255)
                                                                                                                                         aaaGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATctct              <  1:133609/150‑1 (MQ=255)
                                                                                                                                             ggCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcg          <  1:1648342/150‑1 (MQ=255)
                                                                                                                                             ggCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcg          >  1:1561210/1‑150 (MQ=255)
                                                                                                                                              gCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcgc         >  1:550274/1‑150 (MQ=255)
                                                                                                                                              cccccAGAGGCGACCCTTGTTTATAAAACAAGACTATAAAAAATCTTCTTTAGTTTCTGCACCGCCTCCGCTAACATCACCCCACAAACCCGGCGTTTCGTGCGGGTTGCCCAGCACAAACTCACGTACTTTCTTCTCGATCTCTTgcgc         <  2:1716155/146‑1 (MQ=255)
                                                                                                                                               ccGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTcgcg        <  2:1123216/150‑1 (MQ=255)
                                                                                                                                                cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg       >  1:1707811/1‑150 (MQ=255)
                                                                                                                                                cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg       <  2:1414746/150‑1 (MQ=255)
                                                                                                                                                cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg       <  2:1464661/150‑1 (MQ=255)
                                                                                                                                                cGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCgg       <  2:893991/150‑1 (MQ=255)
                                                                                                                                                     aTGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTcg  >  1:1281324/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGAGTTTACGTCGCAGTTCTTGCTCACTGTGATCGCGCACCGCCAGAATGCGTACCGCACGATCCAACAGGCGAGCATATGCCGGGCGACGGGATGTTGATTCTGTCATGGCATATCCTTACAACTTAAAAAAGCAAAAGGGCCGCAGATGCGACCCTTGTGTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCC  >  NC_000913/2822530‑2822828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: