Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,856,258 G→C 7.1% E641Q (GAG→CAG)  fhlA → formate hydrogenlyase transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,856,2580GC 7.1% 421.4 / 18.5 139E641Q (GAG→CAG) fhlAformate hydrogenlyase transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (65/63);  new base C (6/4);  total (71/68)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 143 total aligned reads displayed.

ACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCA  >  NC_000913/2856109‑2856407
                                                                                                                                                     |                                                                                                                                                     
aCATGGAGTGGCCGGGTAACGTACGCGAACTGGTAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTgg                                                                                                                                                       >  1:226933/1‑150 (MQ=255)
    ggAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAggg                                                                                                                                                   <  1:629700/150‑1 (MQ=255)
    ggAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAggg                                                                                                                                                   <  2:27029/150‑1 (MQ=255)
    ggAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAggg                                                                                                                                                   <  1:1178895/150‑1 (MQ=255)
     gAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGc                                                                                                                                                  <  2:1585605/150‑1 (MQ=255)
      aGTTGCCGGGTAACGTACGCGTACGGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCg                                                                                                                                                 <  2:1823934/150‑1 (MQ=255)
       gTGGCCGGGGATCGTCCGCGAACTGGAAACCGTCATTGAGCGCGCGGTATTGCTACCACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGa                                                                                                                                                <  1:1542109/150‑1 (MQ=255)
         ggCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAg                                                                                                                                              >  2:841552/1‑150 (MQ=255)
          gCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGa                                                                                                                                             <  1:1951196/150‑1 (MQ=255)
           ccGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTCCAGGGCGacaac                                                                                                                                            <  2:430020/150‑5 (MQ=255)
               gTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAat                                                                                                                                        <  2:542868/150‑1 (MQ=255)
                    gTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAg                                                                                                                                   <  2:1765624/150‑1 (MQ=255)
                     tACGCGAACTGGAAAACGTCATTGAGCGCTCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGt                                                                                                                                  >  1:1910290/1‑150 (MQ=255)
                      aCGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGtt                                                                                                                                 >  2:1149380/1‑150 (MQ=255)
                      aCGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGGTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGtt                                                                                                                                 <  2:289898/150‑1 (MQ=255)
                       cgcgAACTGGTAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTg                                                                                                                                <  2:558986/150‑1 (MQ=255)
                           aaCTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtg                                                                                                                            <  1:1817344/150‑1 (MQ=255)
                             cTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtgtg                                                                                                                          <  2:1718337/150‑1 (MQ=255)
                             cTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATtgtg                                                                                                                          >  1:603180/1‑150 (MQ=255)
                               ggAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTgcg                                                                                                                        >  2:1293671/1‑150 (MQ=255)
                               ggAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTgcc                                                                                                                        >  1:1712167/1‑149 (MQ=255)
                                 aaaaCGTCATTGAGCGCGCTGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCACATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGcgcg                                                                                                                      <  2:1505940/150‑1 (MQ=255)
                                     gggcATTGTGCGCGAGGTATTGCTAACACGCGGGAACGTGCTGCAGCGGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGGCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCt                                                                                                                  <  1:281766/147‑1 (MQ=255)
                                     cggcATTGAGCGCGCGGTATTGCTACCACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATTAATATCAGTTGATTGTGCGCGTGCt                                                                                                                  <  1:1320022/147‑1 (MQ=255)
                                     cGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCGGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCt                                                                                                                  >  1:1274767/1‑150 (MQ=255)
                                       tCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGCTATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGATGATTGTGCGCGTGCTga                                                                                                                >  1:1416755/1‑150 (MQ=255)
                                        cATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaa                                                                                                               >  1:673230/1‑150 (MQ=255)
                                             aGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaaagaaa                                                                                                          <  2:1644756/150‑1 (MQ=255)
                                              gcgcgcGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAAc                                                                                                         <  2:503211/150‑1 (MQ=255)
                                               cgggcgGTATTGCTAACACGCGGAAACGTGCTGCAGCTGACATTGCCAGATATTGTTTTACCGGAACCTGCAAAGCCGCCTGCAGCAACGGATGTCGCCCTGGAGGGCGAAGATGAATATCAGTTCATTGTGAGCGTGCTGAAAGAAAcc                                                                                                        >  1:109678/4‑150 (MQ=255)
                                                   cgGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACg                                                                                                    >  2:83753/1‑150 (MQ=255)
                                                        ttGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTg                                                                                               <  1:1739410/150‑1 (MQ=255)
                                                         tGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGTTTGTGCGCGTGCTGAATGAAACCAACGGCGTgg                                                                                              <  1:1206089/150‑1 (MQ=255)
                                                         tGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTgg                                                                                              >  1:1055177/1‑150 (MQ=255)
                                                         tGCTAACACGCGGTAACGTGCTGCAGCGGGCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTgg                                                                                              <  2:1818744/150‑1 (MQ=255)
                                                          gCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGt                                                                                             >  2:1575618/1‑150 (MQ=255)
                                                          gCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGt                                                                                             >  1:1489934/1‑150 (MQ=255)
                                                            tAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCACCGGCGTGGTTg                                                                                           >  2:651291/1‑150 (MQ=255)
                                                             aaCACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGc                                                                                          >  2:794271/1‑150 (MQ=255)
                                                               cacGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCg                                                                                        >  2:1197863/1‑150 (MQ=255)
                                                                 cgcgGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCggg                                                                                      >  2:676059/1‑150 (MQ=255)
                                                                 cgcgGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGGGCTGAAAGAAACCAACGGCGTGGTTGCCggg                                                                                      <  1:1866509/150‑1 (MQ=255)
                                                                 cgcgGTAACGTGCTGCAGCGGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGGCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCggg                                                                                      <  1:1928303/150‑1 (MQ=255)
                                                                   cgGTTACGTGCTGCAGCTGTCATTGCCTGATATTGATTTACCGGACCCTGCAACGGCGCCTGCCGCAAGGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTTTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGcc                                                                                    <  1:633593/150‑1 (MQ=255)
                                                                    ggTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCt                                                                                   <  2:936141/150‑1 (MQ=255)
                                                                         cGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAgg                                                                              >  2:1388760/1‑150 (MQ=255)
                                                                               gcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCATTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTgc                                                                        >  2:1745482/1‑150 (MQ=255)
                                                                               gcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTgc                                                                        >  1:45553/1‑150 (MQ=255)
                                                                               gcAGCTGTCAGTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTgc                                                                        >  2:1195768/1‑150 (MQ=255)
                                                                                 aGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGAGTGTGCGCGTGCTGAAAGAACCCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTgcgc                                                                      >  1:1827075/1‑150 (MQ=255)
                                                                                  gCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCa                                                                     >  2:513718/1‑150 (MQ=255)
                                                                                  gCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCa                                                                     >  1:1256238/1‑150 (MQ=255)
                                                                                    tGTCATTGCCAGATATTGTTGTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGAGCGCGTGCTGAAAGAAACCAACGGCGTGGGTGCCGGGCCTAAAGGCGCTGCGCAAc                                                                   <  1:107768/150‑1 (MQ=255)
                                                                                     gTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACg                                                                  >  1:701655/1‑150 (MQ=255)
                                                                                     gTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACg                                                                  <  1:841552/150‑1 (MQ=255)
                                                                                      tCATTGACAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTGGTCGCCCTGGAGGGCGAAGATGAATCTCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGt                                                                 <  1:113689/150‑1 (MQ=255)
                                                                                       cATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTc                                                                >  2:1413374/1‑150 (MQ=255)
                                                                                           gCCAGATATTGTTTTCCCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTggg                                                            <  1:190814/150‑1 (MQ=255)
                                                                                           gCCAGATAGTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGTGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGTCCTAAAGGCGCTGCGCAACGTCTggg                                                            <  2:358864/150‑1 (MQ=255)
                                                                                             cAGATATTGTTTTACCGGACCCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGc                                                          <  1:1321767/150‑1 (MQ=255)
                                                                                              aGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGAGCCTAAAGGCGCTGCGCAACGTCTGGGGCt                                                         >  2:1320576/1‑150 (MQ=255)
                                                                                               gATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTg                                                        >  1:1509204/1‑150 (MQ=255)
                                                                                                atatTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGa                                                       <  1:1136782/150‑1 (MQ=255)
                                                                                                     gTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGc                                                  >  2:1307493/1‑150 (MQ=255)
                                                                                                       tttACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCAc                                                >  1:1028044/1‑150 (MQ=255)
                                                                                                         tACCGGAACCTTAAACGCCGCCTGCCGCCACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACgc                                              >  1:496437/1‑149 (MQ=255)
                                                                                                         tACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAACCCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGa                                              >  1:760810/1‑150 (MQ=255)
                                                                                                          aCCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGAc                                             >  1:511657/1‑150 (MQ=255)
                                                                                                            gggAACCTGAAACGCCGCCTGCCTCAACGGTTCTCGCCCTGGAGGGCGATGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGAccc                                           <  1:1668636/149‑1 (MQ=255)
                                                                                                             ggAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGCAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCAAGACCct                                          >  2:1249062/1‑150 (MQ=255)
                                                                                                               aaCCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCATTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCctgc                                        <  1:560605/150‑1 (MQ=255)
                                                                                                                  cTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGt                                     >  2:1637805/1‑150 (MQ=255)
                                                                                                                  cTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGt                                     >  2:1274510/1‑150 (MQ=255)
                                                                                                                   tGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTc                                    <  1:1411446/150‑1 (MQ=255)
                                                                                                                   tGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTc                                    <  2:209998/150‑1 (MQ=255)
                                                                                                                   tGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTc                                    >  1:1235224/1‑150 (MQ=255)
                                                                                                                      aaCGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACg                                 <  2:573596/150‑1 (MQ=255)
                                                                                                                       aCGCCGCCGGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACgg                                <  1:1387524/150‑1 (MQ=255)
                                                                                                                        cgccgcCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGa                               >  1:393104/1‑150 (MQ=255)
                                                                                                                         gccgccTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGAt                              >  2:711932/1‑150 (MQ=255)
                                                                                                                                   gCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTg                    >  2:71961/1‑150 (MQ=255)
                                                                                                                                   gCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTg                    >  1:1845969/1‑150 (MQ=255)
                                                                                                                                   gCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTg                    >  1:33154/1‑150 (MQ=255)
                                                                                                                                    caccGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTgg                   <  1:1114926/147‑1 (MQ=255)
                                                                                                                                    cAACGGTTGTCGCCCTGGAGGGCGATGATGAATATCAGTTGATTGTGCTCGTGCTGAATGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGAGCAACGTCTGGGGCTGAAACTCACGACCCTGCTGTCACGGATGAAGCGGCTgg                   <  2:109678/150‑1 (MQ=255)
                                                                                                                                      aCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGa                 <  2:701655/150‑1 (MQ=255)
                                                                                                                                       cGGGTGTCGCCCTGGAGGGCGATGATGAATATCAGTGGATTGTGCGCGGGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGaa                <  1:202977/150‑1 (MQ=255)
                                                                                                                                        ggTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAAt               <  1:32167/150‑1 (MQ=255)
                                                                                                                                        catcttCGCCCTCCAGGGCGAAGATGAATATCAGTTGATTGTGCGCGAGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAAt               >  2:303027/6‑150 (MQ=255)
                                                                                                                                         gTTGTCGCCCTGGAGGTCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAAtt              <  2:1061470/150‑1 (MQ=255)
                                                                                                                                         atcttCGCCCTCCAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAAtt              >  2:434006/5‑150 (MQ=255)
                                                                                                                                           ggTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGGTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGa            <  1:1910442/149‑1 (MQ=255)
                                                                                                                                           cttCGCCCTCCAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGa            >  2:406310/3‑150 (MQ=255)
                                                                                                                                            gTCGCCCTGTAGGGCGAAGATGAAAATCAGTTGATTGTGCGCGTGCTGAAATAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGAt           <  2:75095/150‑1 (MQ=255)
                                                                                                                                            gTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAACCCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGAt           >  2:1286838/1‑150 (MQ=255)
                                                                                                                                            gTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGAt           >  1:1495174/1‑150 (MQ=255)
                                                                                                                                             tCGCCCTCCAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATa          >  2:846558/1‑150 (MQ=255)
                                                                                                                                                   tGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAACTCgg    >  1:785942/1‑150 (MQ=255)
                                                                                                                                                    ggAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGc   >  2:220229/1‑150 (MQ=255)
                                                                                                                                                     gAGGGCGAAGATGAATTTCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTTAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCa  <  1:550037/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCA  >  NC_000913/2856109‑2856407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: