Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,870,870 T→A 7.0% E145D (GAA→GAT) ‡ truD ← tRNA(Glu) pseudouridine(13) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,870,8700TA 7.0% 367.7 / 11.9 134E145D (GAA→GAT) ‡truDtRNA(Glu) pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (62/62);  new base A (7/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 139 total aligned reads displayed.

AACCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCT  >  NC_000913/2870721‑2871018
                                                                                                                                                     |                                                                                                                                                    
aaCCAAAACCTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACgttgt                                                                                                                                                      <  1:1670513/150‑1 (MQ=255)
 aCCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACgttgtt                                                                                                                                                     >  1:860529/1‑150 (MQ=255)
     aaaaCTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCTGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAAc                                                                                                                                                 <  1:413528/150‑1 (MQ=255)
         cTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCa                                                                                                                                             >  1:1668247/1‑150 (MQ=255)
          tCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGAACAACGTctg                                                                                                                                            <  2:764679/150‑3 (MQ=255)
             gTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAACCGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGAACAACGTctgatc                                                                                                                                         <  1:1901327/150‑6 (MQ=255)
                 aTTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGAtt                                                                                                                                     >  2:94605/1‑150 (MQ=255)
                 aTTGCGATAGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGAtt                                                                                                                                     <  2:813017/150‑1 (MQ=255)
                    gCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAATCGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCt                                                                                                                                  <  2:1536644/150‑1 (MQ=255)
                     cgctCGGGCACCGGAGTTTTGGTTTGCTACCAGCGCTGCTCCCCCGGCAAGTTGCTAGCGCCAATCCCTAAACGTTGGGCACCGTAGTAGTTCGGTACTCCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTc                                                                                                                                 <  2:373244/147‑1 (MQ=255)
                      gATCGCGCACCGGAGTATTGGTTTGAGCCCAGCGCTGCGCCCCCTGCAAGTAGCTACCGCCAATCCCAAAACCTTGGGAACCGAAGTAGTTCGGTACACCTATTACGCAAATATCGATCAGACGTTGAACAACGTCATCGCGATTGCTCa                                                                                                                                >  2:940285/1‑150 (MQ=255)
                       aTCGCGCACCGGAGTATTGGGTTGCGCCAAGCGCTGCGCCCCCTGCAAGATGCTACCGCCAATCCCAATACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCAc                                                                                                                               <  1:360264/150‑1 (MQ=255)
                            gcACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCATTCCCAAAACGTTGGGCACCGATGTAGTTCGGTACACCTTTTACGCTAATATCGATCAGAGGTGGTTCAACGTCATCGCGATTGCTCAGTTcgc                                                                                                                          >  1:41032/1‑150 (MQ=255)
                             cACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTcgcg                                                                                                                         <  1:1621649/150‑1 (MQ=255)
                              aCCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCgcgc                                                                                                                        <  1:764256/150‑1 (MQ=255)
                              aCCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCgcgc                                                                                                                        <  1:1116710/150‑1 (MQ=255)
                               ccGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCa                                                                                                                       <  1:989264/150‑1 (MQ=255)
                                 ggAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGa                                                                                                                     >  1:803487/1‑150 (MQ=255)
                                  gAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGaa                                                                                                                    <  1:206420/150‑1 (MQ=255)
                                    gTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAAcc                                                                                                                  >  1:1530310/1‑150 (MQ=255)
                                    gTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAAcc                                                                                                                  <  2:1621657/150‑1 (MQ=255)
                                     tATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCa                                                                                                                 >  1:1201329/1‑150 (MQ=255)
                                        tGGATTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAggg                                                                                                              <  1:10925/150‑1 (MQ=255)
                                              gcgcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAgg                                                                                                        <  2:921198/150‑1 (MQ=255)
                                                gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCg                                                                                                      >  2:1359018/1‑150 (MQ=255)
                                                gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCg                                                                                                      <  1:1499464/150‑1 (MQ=255)
                                                gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATAGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAGGCGt                                                                                                     >  1:1460698/1‑150 (MQ=255)
                                                gcCCAGCGCTGATCCCCCAGCAAGTTGCTACCGCCAATCACAAAACCTTGCGCACCGAAGTAGTACGGTACACCTTTTACGCAAATATCGATCCGAAGTTGTTCAACGTCATCGCGCTTGCTCACTTCGCGCATAAACAGGGTAAAGGCg                                                                                                      >  2:1970227/1‑150 (MQ=255)
                                                  ccAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGtt                                                                                                    <  2:322673/150‑1 (MQ=255)
                                                      cgcTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCt                                                                                                >  2:1114813/1‑150 (MQ=255)
                                                       gcTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCtt                                                                                               >  2:1133218/1‑150 (MQ=255)
                                                         tGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTc                                                                                             <  1:1048823/150‑1 (MQ=255)
                                                               cccTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAgcgcg                                                                                       <  1:1888625/150‑1 (MQ=255)
                                                                ccTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAgcgcgc                                                                                      >  1:716226/1‑150 (MQ=255)
                                                                 cTGCAAGTTGCTACCGACAATCCCAAAACGTTGGGCACCGAATTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGcc                                                                                     <  2:469778/150‑1 (MQ=255)
                                                                   gCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTa                                                                                   <  2:1762681/150‑1 (MQ=255)
                                                                     aaGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTaaa                                                                                 <  1:1204501/150‑1 (MQ=255)
                                                                      aGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAAc                                                                                <  1:503824/150‑1 (MQ=255)
                                                                             aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                         >  2:230670/1‑150 (MQ=255)
                                                                             aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                         >  2:39333/1‑150 (MQ=255)
                                                                             aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                         >  1:74465/1‑150 (MQ=255)
                                                                                  cAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCt                                                                    <  1:696358/150‑1 (MQ=255)
                                                                                   aaTCCCAAAACGTTGGGCACCGAAGTAGATCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCtt                                                                   <  1:1992426/150‑1 (MQ=255)
                                                                                    aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                  <  2:1728770/150‑1 (MQ=255)
                                                                                    aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                  >  1:963207/1‑150 (MQ=255)
                                                                                    aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                  <  1:1064343/150‑1 (MQ=255)
                                                                                     tCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                 <  2:763937/150‑1 (MQ=255)
                                                                                     tCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                 >  1:1517980/1‑150 (MQ=255)
                                                                                     tCCCAAAACGTTGGGCACCGAAGTAGTTCGGAACACCTTTTACGCAAATATCGATCAGACGTTGTTCAAAGTCATCGCGATTGCTAACTTCGCGCAGAAACAGGTTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                 >  2:1052221/1‑150 (MQ=255)
                                                                                      cccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgtg                                                                <  2:1622177/150‑1 (MQ=255)
                                                                                       ccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGc                                                               >  2:390484/1‑150 (MQ=255)
                                                                                       ccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGc                                                               >  1:1780380/1‑150 (MQ=255)
                                                                                       ccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAACGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGc                                                               >  2:105996/1‑150 (MQ=255)
                                                                                        cAAAACGGTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCTATCAGACGTTGTTCAACGTCATCGCGATTGCTCTCTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGcc                                                              <  1:375314/150‑1 (MQ=255)
                                                                                          aaaCGTTGGGCACCGAAGTATTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCcgc                                                            <  1:1242953/150‑1 (MQ=255)
                                                                                                   gCACCGAAGTAGATCGTTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGCGTAAAGGCGTTACCTTGCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTcc                                                   <  1:827797/150‑1 (MQ=255)
                                                                                                    cACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGAGCAGAAGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGCTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCa                                                  <  1:1348718/150‑1 (MQ=255)
                                                                                                      ccGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGc                                                <  1:549640/150‑1 (MQ=255)
                                                                                                        gAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGATCCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCAc                                              >  1:112202/1‑150 (MQ=255)
                                                                                                           gtagtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTg                                           <  2:1059211/150‑1 (MQ=255)
                                                                                                             agtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGc                                         <  2:1799986/150‑1 (MQ=255)
                                                                                                              gtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAACCGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGcc                                        >  2:1931811/1‑149 (MQ=255)
                                                                                                               ttCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAg                                       <  1:1575974/150‑1 (MQ=255)
                                                                                                                tCGGTACACCTTTTACGCAAATATCGATCAGACGGTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGc                                      <  1:516806/150‑1 (MQ=255)
                                                                                                                  ggTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCt                                    <  1:905440/150‑1 (MQ=255)
                                                                                                                   gTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCtg                                   >  2:1560394/1‑149 (MQ=255)
                                                                                                                        ccTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGt                              <  1:1966136/150‑1 (MQ=255)
                                                                                                                         cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCCGCACCTGGCAGCCTTCCAGtt                             >  2:632083/1‑150 (MQ=255)
                                                                                                                         cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGtt                             <  1:1122282/150‑1 (MQ=255)
                                                                                                                         cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGtt                             >  1:1291976/1‑150 (MQ=255)
                                                                                                                          ttttACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTg                            >  2:2017496/1‑150 (MQ=255)
                                                                                                                          ttttACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTg                            >  2:1971427/1‑150 (MQ=255)
                                                                                                                             tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                         >  1:1506004/1‑150 (MQ=255)
                                                                                                                             tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                         <  1:526228/150‑1 (MQ=255)
                                                                                                                             tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                         >  1:963869/1‑150 (MQ=255)
                                                                                                                               cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                       >  2:348276/1‑150 (MQ=255)
                                                                                                                               cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                       >  2:835256/1‑150 (MQ=255)
                                                                                                                               cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                       <  1:671299/150‑1 (MQ=255)
                                                                                                                               cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                       >  2:1997200/1‑150 (MQ=255)
                                                                                                                               cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                       >  2:1710294/1‑150 (MQ=255)
                                                                                                                                gCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgtc                      >  1:29600/1‑148 (MQ=255)
                                                                                                                                 cAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCATTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGgcg                     >  1:476988/1‑150 (MQ=255)
                                                                                                                                   aaTATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCt                   <  1:1790661/150‑1 (MQ=255)
                                                                                                                                     tatCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCa                 <  1:1263477/150‑1 (MQ=255)
                                                                                                                                      atCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGACCCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAg                >  2:1085881/1‑150 (MQ=255)
                                                                                                                                      atCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAg                >  1:1617679/1‑150 (MQ=255)
                                                                                                                                         gcgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCGGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc             >  2:478329/6‑150 (MQ=255)
                                                                                                                                         gcgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGATAGGCGATCAGATc             >  1:965942/6‑150 (MQ=255)
                                                                                                                                         gATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc             >  2:1443322/1‑150 (MQ=255)
                                                                                                                                         gATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc             >  1:483254/1‑150 (MQ=255)
                                                                                                                                          cgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGGAAGGCGCTCAGATCg            >  1:444701/5‑150 (MQ=255)
                                                                                                                                          aTCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCg            <  2:1333724/150‑1 (MQ=255)
                                                                                                                                           gatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCgg           >  2:1261249/4‑150 (MQ=255)
                                                                                                                                             tgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAGAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCAGTTGAAAGGCGCTCAGATCGGGCa        >  2:1487343/2‑150 (MQ=255)
                                                                                                                                             aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc         >  1:824755/1‑150 (MQ=255)
                                                                                                                                             aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc         <  1:166/150‑1 (MQ=255)
                                                                                                                                             aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc         <  1:545892/150‑1 (MQ=255)
                                                                                                                                              gACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCa        <  1:1510246/150‑1 (MQ=255)
                                                                                                                                                  ttgttCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTc    >  2:1600581/1‑150 (MQ=255)
                                                                                                                                                    gttCAACGTCATCGCTATTGCTCACATCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCTTTTCCt  <  1:982645/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCT  >  NC_000913/2870721‑2871018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: