Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,870,871 T→A 6.4% E145V (GAA→GTA) ‡ truD ← tRNA(Glu) pseudouridine(13) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,870,8710TA 6.4% 382.3 / 10.2 134E145V (GAA→GTA) ‡truDtRNA(Glu) pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (62/63);  new base A (7/2);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 139 total aligned reads displayed.

ACCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTG  >  NC_000913/2870722‑2871020
                                                                                                                                                     |                                                                                                                                                     
aCCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACgttgtt                                                                                                                                                       >  1:860529/1‑150 (MQ=255)
   aaaaaCTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGTCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCaa                                                                                                                                                    <  1:1815633/150‑1 (MQ=255)
    aaaaCTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCTGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAAc                                                                                                                                                   <  1:413528/150‑1 (MQ=255)
        cTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCa                                                                                                                                               >  1:1668247/1‑150 (MQ=255)
         tCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGAACAACGTctg                                                                                                                                              <  2:764679/150‑3 (MQ=255)
            gTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAACCGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGAACAACGTctgatc                                                                                                                                           <  1:1901327/150‑6 (MQ=255)
                aTTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGAtt                                                                                                                                       >  2:94605/1‑150 (MQ=255)
                aTTGCGATAGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGAtt                                                                                                                                       <  2:813017/150‑1 (MQ=255)
                   gCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAATCGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCt                                                                                                                                    <  2:1536644/150‑1 (MQ=255)
                   gCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCt                                                                                                                                    <  1:1968472/150‑1 (MQ=255)
                    cgctCGGGCACCGGAGTTTTGGTTTGCTACCAGCGCTGCTCCCCCGGCAAGTTGCTAGCGCCAATCCCTAAACGTTGGGCACCGTAGTAGTTCGGTACTCCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTc                                                                                                                                   <  2:373244/147‑1 (MQ=255)
                     gATCGCGCACCGGAGTATTGGTTTGAGCCCAGCGCTGCGCCCCCTGCAAGTAGCTACCGCCAATCCCAAAACCTTGGGAACCGAAGTAGTTCGGTACACCTATTACGCAAATATCGATCAGACGTTGAACAACGTCATCGCGATTGCTCa                                                                                                                                  >  2:940285/1‑150 (MQ=255)
                      aTCGCGCACCGGAGTATTGGGTTGCGCCAAGCGCTGCGCCCCCTGCAAGATGCTACCGCCAATCCCAATACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCAc                                                                                                                                 <  1:360264/150‑1 (MQ=255)
                           gcACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCATTCCCAAAACGTTGGGCACCGATGTAGTTCGGTACACCTTTTACGCTAATATCGATCAGAGGTGGTTCAACGTCATCGCGATTGCTCAGTTcgc                                                                                                                            >  1:41032/1‑150 (MQ=255)
                            cACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTcgcg                                                                                                                           <  1:1621649/150‑1 (MQ=255)
                             aCCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCgcgc                                                                                                                          <  1:1116710/150‑1 (MQ=255)
                             aCCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCgcgc                                                                                                                          <  1:764256/150‑1 (MQ=255)
                              ccGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCa                                                                                                                         <  1:989264/150‑1 (MQ=255)
                                ggAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGa                                                                                                                       >  1:803487/1‑150 (MQ=255)
                                 gAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGaa                                                                                                                      <  1:206420/150‑1 (MQ=255)
                                   gTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAAcc                                                                                                                    <  2:1621657/150‑1 (MQ=255)
                                   gTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAAcc                                                                                                                    >  1:1530310/1‑150 (MQ=255)
                                    tATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCa                                                                                                                   >  1:1201329/1‑150 (MQ=255)
                                       tGGATTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAggg                                                                                                                <  1:10925/150‑1 (MQ=255)
                                             gcgcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAgg                                                                                                          <  2:921198/150‑1 (MQ=255)
                                               gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCg                                                                                                        <  1:1499464/150‑1 (MQ=255)
                                               gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCg                                                                                                        >  2:1359018/1‑150 (MQ=255)
                                               gcCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATAGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAGGCGt                                                                                                       >  1:1460698/1‑150 (MQ=255)
                                               gcCCAGCGCTGATCCCCCAGCAAGTTGCTACCGCCAATCACAAAACCTTGCGCACCGAAGTAGTACGGTACACCTTTTACGCAAATATCGATCCGAAGTTGTTCAACGTCATCGCGCTTGCTCACTTCGCGCATAAACAGGGTAAAGGCg                                                                                                        >  2:1970227/1‑150 (MQ=255)
                                                 ccAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGtt                                                                                                      <  2:322673/150‑1 (MQ=255)
                                                     ggctgctCACCATGCAAGTTGCTACCGCCAATCCCAATACGTTGGTCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCTGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCt                                                                                                  <  1:223676/149‑1 (MQ=255)
                                                     cgcTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCt                                                                                                  >  2:1114813/1‑150 (MQ=255)
                                                      gcTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCtt                                                                                                 >  2:1133218/1‑150 (MQ=255)
                                                        tGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTc                                                                                               <  1:1048823/150‑1 (MQ=255)
                                                               ccTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAgcgcgc                                                                                        >  1:716226/1‑150 (MQ=255)
                                                                cTGCAAGTTGCTACCGACAATCCCAAAACGTTGGGCACCGAATTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGcc                                                                                       <  2:469778/150‑1 (MQ=255)
                                                                  gCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTa                                                                                     <  2:1762681/150‑1 (MQ=255)
                                                                    aaGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTaaa                                                                                   <  1:1204501/150‑1 (MQ=255)
                                                                     aGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAAc                                                                                  <  1:503824/150‑1 (MQ=255)
                                                                            aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                           >  2:230670/1‑150 (MQ=255)
                                                                            aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                           >  2:39333/1‑150 (MQ=255)
                                                                            aCCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCtt                                                                           >  1:74465/1‑150 (MQ=255)
                                                                                 cAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCt                                                                      <  1:696358/150‑1 (MQ=255)
                                                                                   aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                    >  1:963207/1‑150 (MQ=255)
                                                                                   aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                    <  2:1728770/150‑1 (MQ=255)
                                                                                   aTCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtg                                                                    <  1:1064343/150‑1 (MQ=255)
                                                                                    tCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                   <  2:763937/150‑1 (MQ=255)
                                                                                    tCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                   >  1:1517980/1‑150 (MQ=255)
                                                                                    tCCCAAAACGTTGGGCACCGAAGTAGTTCGGAACACCTTTTACGCAAATATCGATCAGACGTTGTTCAAAGTCATCGCGATTGCTAACTTCGCGCAGAAACAGGTTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgt                                                                   >  2:1052221/1‑150 (MQ=255)
                                                                                     cccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTtgtg                                                                  <  2:1622177/150‑1 (MQ=255)
                                                                                      ccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGc                                                                 >  2:390484/1‑150 (MQ=255)
                                                                                      ccAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAACGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGc                                                                 >  2:105996/1‑150 (MQ=255)
                                                                                       cAAAACGGTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCTATCAGACGTTGTTCAACGTCATCGCGATTGCTCTCTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGcc                                                                <  1:375314/150‑1 (MQ=255)
                                                                                         aaaCGTTGGGCACCGAAGTATTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCcgc                                                              <  1:1242953/150‑1 (MQ=255)
                                                                                                  gCACCGAAGTAGATCGTTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGCGTAAAGGCGTTACCTTGCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTcc                                                     <  1:827797/150‑1 (MQ=255)
                                                                                                   cACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGAGCAGAAGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGCTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCa                                                    <  1:1348718/150‑1 (MQ=255)
                                                                                                     ccGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGc                                                  <  1:549640/150‑1 (MQ=255)
                                                                                                       gAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGATCCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCAc                                                >  1:112202/1‑150 (MQ=255)
                                                                                                          gtagtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTg                                             <  2:1059211/150‑1 (MQ=255)
                                                                                                            agtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGc                                           <  2:1799986/150‑1 (MQ=255)
                                                                                                             gtTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAACCGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGcc                                          >  2:1931811/1‑149 (MQ=255)
                                                                                                              ttCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAg                                         <  1:1575974/150‑1 (MQ=255)
                                                                                                               tCGGTACACCTTTTACGCAAATATCGATCAGACGGTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGc                                        <  1:516806/150‑1 (MQ=255)
                                                                                                                 ggTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCt                                      <  1:905440/150‑1 (MQ=255)
                                                                                                                  gTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCtg                                     >  2:1560394/1‑149 (MQ=255)
                                                                                                                       ccTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGt                                <  1:1966136/150‑1 (MQ=255)
                                                                                                                        cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCCGCACCTGGCAGCCTTCCAGtt                               >  2:632083/1‑150 (MQ=255)
                                                                                                                        cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGtt                               <  1:1122282/150‑1 (MQ=255)
                                                                                                                        cTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGtt                               >  1:1291976/1‑150 (MQ=255)
                                                                                                                         ttttACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTg                              >  2:2017496/1‑150 (MQ=255)
                                                                                                                         ttttACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTg                              >  2:1971427/1‑150 (MQ=255)
                                                                                                                            tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                           >  1:1506004/1‑150 (MQ=255)
                                                                                                                            tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                           >  1:963869/1‑150 (MQ=255)
                                                                                                                            tACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGaaa                           <  1:526228/150‑1 (MQ=255)
                                                                                                                              cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                         >  2:348276/1‑150 (MQ=255)
                                                                                                                              cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                         >  2:1997200/1‑150 (MQ=255)
                                                                                                                              cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                         >  2:835256/1‑150 (MQ=255)
                                                                                                                              cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                         <  1:671299/150‑1 (MQ=255)
                                                                                                                              cGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAgg                         >  2:1710294/1‑150 (MQ=255)
                                                                                                                                cAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCATTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGgcg                       >  1:476988/1‑150 (MQ=255)
                                                                                                                                  aaTATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCt                     <  1:1790661/150‑1 (MQ=255)
                                                                                                                                    tatCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCa                   <  1:1263477/150‑1 (MQ=255)
                                                                                                                                     atCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGACCCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAg                  >  2:1085881/1‑150 (MQ=255)
                                                                                                                                     atCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAg                  >  1:1617679/1‑150 (MQ=255)
                                                                                                                                        gcgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCGGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc               >  2:478329/6‑150 (MQ=255)
                                                                                                                                        gcgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGATAGGCGATCAGATc               >  1:965942/6‑150 (MQ=255)
                                                                                                                                        gcgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc               >  1:1674557/6‑150 (MQ=255)
                                                                                                                                        gATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc               >  2:1443322/1‑150 (MQ=255)
                                                                                                                                        gATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATc               >  1:483254/1‑150 (MQ=255)
                                                                                                                                         cgatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGGAAGGCGCTCAGATCg              >  1:444701/5‑150 (MQ=255)
                                                                                                                                         aTCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCg              <  2:1333724/150‑1 (MQ=255)
                                                                                                                                          gatgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCgg             >  2:1261249/4‑150 (MQ=255)
                                                                                                                                            tgACGTTGAACAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAGAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCAGTTGAAAGGCGCTCAGATCGGGCa          >  2:1487343/2‑150 (MQ=255)
                                                                                                                                            aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc           >  1:824755/1‑150 (MQ=255)
                                                                                                                                            aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc           <  1:545892/150‑1 (MQ=255)
                                                                                                                                            aGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGc           <  1:166/150‑1 (MQ=255)
                                                                                                                                             gACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCa          <  1:1510246/150‑1 (MQ=255)
                                                                                                                                                 ttgttCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTc      >  2:1600581/1‑150 (MQ=255)
                                                                                                                                                   gttCAACGTCATCGCTATTGCTCACATCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCTTTTCCt    <  1:982645/150‑1 (MQ=255)
                                                                                                                                                     tCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTTAAAGGCGCTCAGATCGGGCATTTCCtag  <  1:312634/150‑3 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCAAAAACTCCGTTTATTGCGATCGCGCACCGGAGTATTGGTTTGCGCCCAGCGCTGCGCCCCCTGCAAGTTGCTACCGCCAATCCCAAAACGTTGGGCACCGAAGTAGTTCGGTACACCTTTTACGCAAATATCGATCAGACGTTGTTCAACGTCATCGCGATTGCTCACTTCGCGCAGAACCAGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTG  >  NC_000913/2870722‑2871020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: