Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,890,619 T→A 5.2% D427V (GAC→GTC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,890,6190TA 5.2% 338.7 / 6.5 126D427V (GAC→GTC) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (68/50);  new base A (3/4);  total (71/55)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 127 total aligned reads displayed.

CGGCGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGA  >  NC_000913/2890470‑2890765
                                                                                                                                                     |                                                                                                                                                  
cggcggCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCAGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGt                                                                                                                                                    <  2:1527357/150‑1 (MQ=255)
   cggcgCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAg                                                                                                                                                 <  2:674115/150‑1 (MQ=255)
     gcgcGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCg                                                                                                                                               >  1:1511956/1‑150 (MQ=255)
       gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAg                                                                                                                                             <  1:1055874/150‑1 (MQ=255)
            gctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGCCTTCGCCCTCTTCTTCCCCGCGGTCAGCGAGGAAGc                                                                                                                                        >  1:498764/1‑150 (MQ=255)
            gctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAga                                                                                                                                        <  1:1574710/150‑2 (MQ=255)
            gctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGc                                                                                                                                        >  2:92940/1‑150 (MQ=255)
             ctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGCGGTATCTGGATTGGCTGGCAGGCGGAAGTAAACGTTATCTTCGCTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGCTCAGCGAGGAAGCt                                                                                                                                       >  1:1249303/1‑150 (MQ=255)
              tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTg                                                                                                                                      >  1:1958677/1‑150 (MQ=255)
               gcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCCCGCGGTCAGCGAGGAAGCTgg                                                                                                                                     >  2:1204870/1‑150 (MQ=255)
                cATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTTTCGTGAGGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                    >  1:1255466/1‑150 (MQ=255)
                cATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaagag                                                                                                                                    <  1:754218/150‑6 (MQ=255)
                cATAAAGGCGCGGAACGGCGCAATACCGGTGCCAGGGCCAATCATAAAAACCGGGTTTTCTGTATTGGCTTGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGAGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGTAAGCTGGa                                                                                                                                    >  2:183740/1‑150 (MQ=255)
                aaTAAAGGCGCGGAACTGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaagag                                                                                                                                    <  2:1341281/149‑6 (MQ=255)
                 atatAGGCGCGGAACGGCGCAATACCGGTGCCTGTGCCAATCATAATCACCGTGTTTTCTGGCTTGGCTGGCAGGAGTAAGTTATCTTTATGTTCTATAAATACGAGGACTTCGCCATCTTCTTCCACGCGTTCAGCGAGGAAGCTGGAg                                                                                                                                   <  1:1091705/150‑1 (MQ=255)
                 aaaaaGGCGCGGAACTGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                   <  1:1893939/148‑1 (MQ=255)
                 aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                   <  2:537120/150‑1 (MQ=255)
                 aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                   >  1:1651305/1‑150 (MQ=255)
                  tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaagaggg                                                                                                                                  <  2:1837386/150‑8 (MQ=255)
                  tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAgg                                                                                                                                  >  2:675813/1‑150 (MQ=255)
                  tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAgg                                                                                                                                  <  2:1705134/150‑1 (MQ=255)
                   aaaGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTGCTGGATTGGCTGGCATGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGc                                                                                                                                 <  2:290889/150‑1 (MQ=255)
                    aaGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGca                                                                                                                                <  2:1418188/150‑1 (MQ=255)
                      ggCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcacc                                                                                                                              <  1:1005335/150‑1 (MQ=255)
                        cgcgGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcaccac                                                                                                                            >  2:1130866/1‑150 (MQ=255)
                        cgcgGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGcaccac                                                                                                                            >  1:26595/1‑150 (MQ=255)
                           ggAACGGCGCAATACCGGTGCCTGGGCCAATCATAAGCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCgg                                                                                                                         <  1:1044286/150‑1 (MQ=255)
                             aaCGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCa                                                                                                                       <  2:4821/150‑1 (MQ=255)
                                ggCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACgg                                                                                                                    >  1:735081/1‑150 (MQ=255)
                                 gcgcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACggg                                                                                                                   >  1:1707931/1‑150 (MQ=255)
                                 gcgcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACggg                                                                                                                   >  1:1908688/1‑150 (MQ=255)
                                  cgcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCCCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgc                                                                                                                  >  1:1802468/1‑150 (MQ=255)
                                     aaTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGcgcg                                                                                                               >  2:445125/1‑150 (MQ=255)
                                      aTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCgg                                                                                                              <  2:696097/150‑1 (MQ=255)
                                      aTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCgg                                                                                                              <  1:1618313/150‑1 (MQ=255)
                                         ccGGTGCCGGGGCCAATCATAATCACCTGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCTATAAATACGCGGACTTCGCCCTCTTCATCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCt                                                                                                           <  2:478603/150‑1 (MQ=255)
                                          cgctGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCtt                                                                                                          <  1:1533854/147‑1 (MQ=255)
                                              gCCTGGGCCACTCAACATCACCGGGGTTTCTGGCTTGGCTGGCAGGCGGAAGATATCGTTATGTTCGATAAATCCGCGGACTTCGCCCTCTTCTTCCACGCGCTCTGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAc                                                                                                      <  1:78776/150‑1 (MQ=255)
                                                    gCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGCGGCCCCCCCGGCCCGGGCGCGGCCTTCCACGTCGTa                                                                                                >  1:1434756/1‑150 (MQ=255)
                                                    gCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTa                                                                                                >  1:1130361/1‑150 (MQ=255)
                                                      cAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                              <  1:497483/150‑1 (MQ=255)
                                                      cAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGGTCGATAAATACGCGGACTTCGCCCGCTTCTTCCACGCGGTCACCGAGGAAGCTGGAGGCACCCCCGGCCCCGGCGCGGCCTTCCACGTCGTAAc                                                                                              >  2:1264165/1‑150 (MQ=255)
                                                        aTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTTGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGc                                                                                            >  2:1990069/1‑150 (MQ=255)
                                                          cATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcac                                                                                          >  1:1497428/1‑150 (MQ=255)
                                                           aTAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcacc                                                                                         >  1:877673/1‑150 (MQ=255)
                                                             aaTCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccac                                                                                       <  1:1764582/150‑1 (MQ=255)
                                                                cACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccacacc                                                                                    <  2:1059794/150‑1 (MQ=255)
                                                                    ggggTTTCTGGATTGGTTGGCAGGGGGAAGTTATCGTTATGTTCTATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGGACCACACCAACg                                                                                <  2:1249303/150‑1 (MQ=255)
                                                                                   gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCg                                                                 >  1:1782667/1‑150 (MQ=255)
                                                                                   gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCg                                                                 <  2:1760031/150‑1 (MQ=255)
                                                                                      ggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtc                                                              >  1:1850932/1‑150 (MQ=255)
                                                                                      ggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGGCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtc                                                              <  2:172461/150‑1 (MQ=255)
                                                                                        cAGGCGGAAGATTTCGTTATGTTCGATAAATACGCGTACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCAGCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCTCACCAACGGTGACGTGTACTTCGTtctc                                                            <  1:281966/150‑1 (MQ=255)
                                                                                         aGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGGGTACTTCGTTCTCg                                                           <  2:1319268/150‑1 (MQ=255)
                                                                                         aGGCGGAAGGTATCGTTATGATCGATAAATACGCTGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCg                                                           <  2:330459/150‑1 (MQ=255)
                                                                                          ggCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCgc                                                          >  1:871509/1‑149 (MQ=255)
                                                                                           gCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGAc                                                         <  2:1731857/150‑1 (MQ=255)
                                                                                                 gTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGc                                                   >  2:1762795/1‑150 (MQ=255)
                                                                                                  ttATCGTTATGTTCGATAAATACGCGGTCTTCGCCCTCTTCTTCCACTCGGTCATCTAGGAATATGGAGTCACAACCGTCACGGGCGCGGCCTTCCACGTCGTAACGCAGCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGcc                                                  >  1:1393931/1‑150 (MQ=255)
                                                                                                    aTCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCCCGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTg                                                >  2:1118525/1‑150 (MQ=255)
                                                                                                     tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGc                                               >  2:1245332/1‑150 (MQ=255)
                                                                                                       gTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGATGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGAGACGTGTACTTCGTTCTCGACTTCCGCCTGCgc                                             >  1:454214/1‑149 (MQ=255)
                                                                                                           tGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgaggag                                         >  2:768897/1‑150 (MQ=255)
                                                                                                            gtgcGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTACCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                        <  1:1010738/147‑1 (MQ=255)
                                                                                                            gatCGATAAATACGCGGACTACGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCTCGGCCTTACACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                        >  1:1386764/3‑150 (MQ=255)
                                                                                                            gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                        <  2:1506962/150‑1 (MQ=255)
                                                                                                            gTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                        >  1:485872/1‑150 (MQ=255)
                                                                                                               cGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                     >  2:926679/1‑150 (MQ=255)
                                                                                                                    aaTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGCCTTCCGCCTGCGAGGAGGCGATGGaa                                >  1:117422/1‑150 (MQ=255)
                                                                                                                      tACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                              >  1:1495122/1‑150 (MQ=255)
                                                                                                                       aCGCGGACTTCGCTCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCCCCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATAc                             >  2:772719/1‑150 (MQ=255)
                                                                                                                       aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATAc                             <  1:1747846/150‑1 (MQ=255)
                                                                                                                        cgcgGCATTCGCCCTCTTCTTCCACTCGGTCAGCGATGAAGCTGGACGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACTCACCACACCAACGGTGACGTGTACTTCGTTCTCGTCTTCCGCCTGCGAGGAGGCGATGGAATACa                            <  2:309716/150‑1 (MQ=255)
                                                                                                                        cgcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCCCCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                            <  2:1511956/150‑1 (MQ=255)
                                                                                                                        cgcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCCCGTCGTACCGCACCACACCACCGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATAcc                            >  1:140546/1‑149 (MQ=255)
                                                                                                                        cgcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                            <  2:1156716/150‑1 (MQ=255)
                                                                                                                        cgcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                            >  1:1914948/1‑150 (MQ=255)
                                                                                                                        cgcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                            >  1:638442/1‑150 (MQ=255)
                                                                                                                         gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAg                           >  1:1073136/1‑150 (MQ=255)
                                                                                                                          cgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGa                          <  2:1908688/150‑1 (MQ=255)
                                                                                                                             aCTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTGTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                       >  1:1106455/1‑150 (MQ=255)
                                                                                                                              cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCCCCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTACGCCTGCGAGGAGGCCTAGGAATACAGAcgcg                      >  2:195125/1‑150 (MQ=255)
                                                                                                                              cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                      <  2:1889981/150‑1 (MQ=255)
                                                                                                                                tCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGCTGGAAGACAGACgcggc                    >  1:317387/1‑150 (MQ=255)
                                                                                                                                tCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                    <  2:960487/150‑1 (MQ=255)
                                                                                                                                tCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                    >  2:74439/1‑150 (MQ=255)
                                                                                                                                  gCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTACCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGCCGCGGCGt                  >  2:392065/1‑150 (MQ=255)
                                                                                                                                   cccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCCCCGGCACGGGCGCGGCCTTCCACGTCGTACCGCACCACACCCACTGTGACGGGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTc                 <  2:1255466/150‑1 (MQ=255)
                                                                                                                                    ctccagcttcCTCGCTGACCGCGTGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCCCGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTcc                >  2:1017639/7‑149 (MQ=255)
                                                                                                                                    ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCa                <  2:26595/150‑1 (MQ=255)
                                                                                                                                      tcttctTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGc              <  2:1713979/150‑1 (MQ=255)
                                                                                                                                        ttcttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCgg            >  2:764058/1‑150 (MQ=255)
                                                                                                                                        agcttcCTCGCTGACCGCGTGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTACCGCACCACACCAACGGGGTCGTGTCCTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAAGTCCGACGCGGCGTCAGCgg            >  2:274750/3‑150 (MQ=255)
                                                                                                                                         tcttcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGACTGCGTGGAGGCGATGGTATACAGACGCGGCGTCAGCggg           >  1:1263165/1‑150 (MQ=255)
                                                                                                                                            tcCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGgcgc        >  1:1503052/1‑150 (MQ=255)
                                                                                                                                              cACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag      >  1:666287/1‑150 (MQ=255)
                                                                                                                                                cgcgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcagca    >  2:654203/1‑150 (MQ=255)
                                                                                                                                                 gcgGTCAGCGAGGAAGCTGGAGGCACCTCCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGcagcag   <  1:636418/150‑1 (MQ=255)
                                                                                                                                                 gcgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGAGAGGAGGCGATGGAATACAGACGCGGCGTCATCGGGCGcagcag   <  2:1620266/150‑1 (MQ=255)
                                                                                                                                                  cgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa  >  2:1068641/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGCGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGA  >  NC_000913/2890470‑2890765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: