Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,327 A→C 6.5% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3270AC 6.5% 415.5 / 15.1 139intergenic (‑54/‑265)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (57/73);  new base C (4/5);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 154 total aligned reads displayed.

CTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACAC  >  NC_000913/2900178‑2900476
                                                                                                                                                       |                                                                                                                                                     
cTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCga                                                                                                                                                       >  1:1743099/1‑150 (MQ=255)
cTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCga                                                                                                                                                       <  1:566715/150‑1 (MQ=255)
 tGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCgag                                                                                                                                                      >  1:815946/1‑150 (MQ=255)
   gCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAGTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCgaggg                                                                                                                                                    >  1:1903866/1‑148 (MQ=255)
   gCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAAGAAAGGTAACTTCCCCCGCgag                                                                                                                                                      <  1:947835/150‑1 (MQ=255)
    ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAGAAAGGTAACTTCCCCCGCGagag                                                                                                                                                    >  1:1186936/1‑150 (MQ=255)
    ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGCGAGAAATAATCCATTGAGAACGCATTGAGAGTTTCGTTTGACATAGTTAGCTCCAATTTCCGAAACGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                                   <  2:71239/150‑1 (MQ=255)
      tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGa                                                                                                                                                 <  2:1012834/150‑1 (MQ=255)
      tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGa                                                                                                                                                 >  1:1244340/1‑150 (MQ=255)
       aaaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                                                                                                                <  1:274660/150‑1 (MQ=255)
        aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGt                                                                                                                                              >  1:897432/1‑150 (MQ=255)
            gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAc                                                                                                                                           >  1:1726126/1‑150 (MQ=255)
             cTATTCCCAACGGTAACAATTGCGGTTTTACCTTTCAGGGATAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                                                                                                          <  1:1224228/150‑1 (MQ=255)
              taatCCCACAGTTAACAATTGCGGTTTTACCTTTCATGGAGAAAAACACCATTTAGAACGCATTGATAGATTCGCTTGACATAGTTAGCTCCAATTTCCGTAAGGA‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCTGGGGAAGTTACCt                                                                                                                                         <  1:990242/147‑1 (MQ=255)
              tATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                         <  1:1722654/150‑1 (MQ=255)
               aTTCCCACCGGTAACAATTTCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCGCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCtt                                                                                                                                        <  1:1577475/150‑1 (MQ=39)
                 tCCCACCGGTAACAATTGGGTTTTTACCTTTCAGGGAGAAAAAATCCATTGAGTACGCATTGAGAGATTCGATTGACATATTTAGCTCCAATTTCCTTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGATGGAAGTTACCTTtc                                                                                                                                      <  2:1707755/150‑1 (MQ=255)
                 tCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtc                                                                                                                                      >  1:1514752/1‑150 (MQ=255)
                   ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                    <  1:691999/150‑1 (MQ=255)
                      ccGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAtt                                                                                                                                 <  2:338400/150‑1 (MQ=255)
                            aCAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATGGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAACAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATctct                                                                                                                           <  1:1511498/150‑1 (MQ=255)
                             cAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                                                                                                          >  1:606439/1‑150 (MQ=255)
                             cAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                                                                                                          <  1:1419253/150‑1 (MQ=255)
                              aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                         >  1:417551/1‑150 (MQ=255)
                                ttGCGGTTTTACCTTTCAGGTAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAAATTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                       <  2:229063/150‑1 (MQ=255)
                                ttGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTa                                                                                                                       >  1:1793781/1‑150 (MQ=255)
                                  gCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAc                                                                                                                     >  2:1328394/1‑150 (MQ=255)
                                    ggTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTg                                                                                                                   <  1:266890/150‑1 (MQ=255)
                                    ggTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATATCTGGTAACTg                                                                                                                   >  1:183733/1‑150 (MQ=255)
                                      ttttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTgg                                                                                                                  <  2:695076/150‑1 (MQ=255)
                                            cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                           >  2:22571/1‑150 (MQ=255)
                                              ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                         >  1:1981757/1‑150 (MQ=255)
                                              ttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATAAtga                                                                                                         <  2:1235462/150‑3 (MQ=255)
                                                cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt                                                                                                       <  2:525551/150‑1 (MQ=255)
                                                  gtgagaAAAAATACATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGTATGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                     <  1:43722/148‑1 (MQ=255)
                                                  gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCCAGTTACCAGAGATAAtgagaaa                                                                                                     <  1:185904/150‑7 (MQ=255)
                                                  gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAAAGGGAAGTTACCTTTCTCAATATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                     >  2:305872/1‑150 (MQ=255)
                                                   ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGc                                                                                                    <  2:1449082/150‑1 (MQ=255)
                                                   ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGAAATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                     <  1:1592457/150‑1 (MQ=255)
                                                    gagaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAAATTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCa                                                                                                   <  1:633331/150‑1 (MQ=255)
                                                          aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                            <  2:897432/150‑1 (MQ=255)
                                                             tCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                          <  2:215683/150‑1 (MQ=255)
                                                              ccATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATa                                                                                         <  2:1163066/150‑1 (MQ=255)
                                                               cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                        >  2:1392322/1‑150 (MQ=255)
                                                               cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAc                                                                                       >  1:1693987/1‑150 (MQ=255)
                                                                     gaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTa                                                                                  <  2:182925/150‑1 (MQ=255)
                                                                        cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtt                                                                               >  1:1498661/1‑149 (MQ=255)
                                                                        cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                               <  2:1861768/150‑1 (MQ=255)
                                                                        cGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                               <  2:860308/150‑1 (MQ=255)
                                                                        cGCATTGAGAGATTCGATTGACATAGTTACCTCCAATTTCCGAAACGC‑‑AAAAAAAAAGAAAGGGCACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATACGGGCATAACTGCTAAtg                                                                               <  2:281230/150‑1 (MQ=255)
                                                                         gCATTGAGAGATTCGATTGACATAGTTAGGTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAGTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgt                                                                              >  1:319292/1‑150 (MQ=255)
                                                                           aTTGAGAGATTCGATTGACATAGTTAGATCCAATTTCCGTAAGGC‑‑AAATAAAAAGAAAGGTAACTTCACCCGCTAGAGGGAAGTTACCTTTCTCATTATATATGGTTACTGGAGATAATTTTTAGAAGAAGGGCATAAATGATAATTTGa                                                                            >  1:129205/1‑150 (MQ=255)
                                                                            ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                                                                           <  2:209468/150‑1 (MQ=255)
                                                                             tGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                          <  2:770245/150‑1 (MQ=255)
                                                                              tagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                         <  2:1608142/149‑1 (MQ=255)
                                                                              gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCg                                                                         >  2:870786/1‑150 (MQ=255)
                                                                                gagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTa                                                                       >  1:848573/1‑150 (MQ=255)
                                                                                  taTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                     <  2:1829662/149‑1 (MQ=255)
                                                                                      cGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAg                                                                <  2:568164/150‑1 (MQ=255)
                                                                                           gACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                            <  2:1723025/150‑1 (MQ=255)
                                                                                            aCATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa                                                           <  2:68922/150‑1 (MQ=255)
                                                                                            aCATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa                                                           <  2:417551/150‑1 (MQ=255)
                                                                                             cATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGat                                                          >  1:909765/1‑150 (MQ=255)
                                                                                               tAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGatat                                                        >  1:1923191/1‑150 (MQ=255)
                                                                                                    aGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTTTCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa                                                   >  2:1750093/1‑150 (MQ=255)
                                                                                                       tCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTg                                                >  1:1012560/1‑150 (MQ=255)
                                                                                                        ccAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                               >  1:1683442/1‑150 (MQ=255)
                                                                                                         cAATTTCCGTAAGGC‑‑AAAAAAAAAGAAATGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                              <  2:1752195/150‑1 (MQ=255)
                                                                                                         cAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTc                                              <  2:1983942/150‑1 (MQ=255)
                                                                                                          aaTTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                             >  2:366302/1‑150 (MQ=255)
                                                                                                            tttCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCAAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCaaa                                           <  1:869625/150‑1 (MQ=255)
                                                                                                              tCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                         >  1:330811/1‑150 (MQ=255)
                                                                                                               ccGTAAGGCAAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaata                                         >  2:112609/1‑150 (MQ=255)
                                                                                                                   aaGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGGGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtttt                                    <  2:1028538/150‑1 (MQ=255)
                                                                                                                    aGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTa                                   <  1:1368400/150‑1 (MQ=255)
                                                                                                                      gC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                                 >  1:1366611/1‑150 (MQ=255)
                                                                                                                      gC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                                 >  2:535750/1‑150 (MQ=255)
                                                                                                                      gC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                                 <  1:1278792/150‑1 (MQ=255)
                                                                                                                      gC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                                 <  1:1198410/150‑1 (MQ=255)
                                                                                                                           aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                              >  2:1964132/1‑150 (MQ=255)
                                                                                                                            aaaaaaaGAAAGGTATCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAACACACTGTGATATTAGTATAGACAAATAATTTTAAAAAGc                             >  1:158772/1‑150 (MQ=255)
                                                                                                                            aaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAGCTTTCTCATTATCTCTGGTAACTGGAGATAATTTATAGCATAAGGGCATAAGAGCTAATGAGATCGTAATCACAGTGTGATATTCGTATTCTCAAATAATTTTAAAAAGc                             >  1:819863/1‑150 (MQ=255)
                                                                                                                                aaagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGaa                         >  1:1489402/1‑150 (MQ=255)
                                                                                                                                     aagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg                    >  2:488144/1‑150 (MQ=255)
                                                                                                                                      agGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGa                   >  2:1663228/1‑150 (MQ=255)
                                                                                                                                       ggTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt                  >  2:1478946/1‑150 (MQ=255)
                                                                                                                                        gTAACTTCCCTCTCGCGGGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAtt                 >  1:15317/1‑150 (MQ=255)
                                                                                                                                        gTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTAATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATAGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAtt                 <  1:1244912/150‑1 (MQ=255)
                                                                                                                                         tAACTTCCCCCGCGAGAGTGAAGTTAACTTTCTCATTATCTTTGGTAACTTGATATAATTTTTAGAATAAGGTCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTa                <  1:1240864/150‑1 (MQ=255)
                                                                                                                                         tAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGTTAACTGGAGATAAGTTTTAGCAGAAGGGCATAACTGATAATGTGATCGTAATCACAGTGTGAGATTCGTATTGTCAAATAATGTTAAAAAGCAGAAGCGTGATTa                >  1:1974122/1‑150 (MQ=255)
                                                                                                                                           aCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTAct              >  2:1343508/1‑150 (MQ=255)
                                                                                                                                             ttCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActct            <  2:606439/150‑1 (MQ=255)
                                                                                                                                              tcaccagcgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTTGAGATAATTTTTTGCATAATGGCATAACTGATAATGTGATCGTAATCACAGTGTGATATTAGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc           <  2:419187/144‑1 (MQ=255)
                                                                                                                                              tCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACtctc           <  1:1820812/150‑1 (MQ=255)
                                                                                                                                               cccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTca          >  1:1943988/1‑150 (MQ=255)
                                                                                                                                                ccccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcac         <  1:535255/150‑1 (MQ=255)
                                                                                                                                                   cgagagaGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcacaca      <  2:1398925/147‑1 (MQ=255)
                                                                                                                                                      gagaGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTAAAAAGCAGAAGCGTGATTACTCTCACACAacac  <  1:218476/150‑1 (MQ=255)
                                                                                                                                                      gagaGGGAAGTTACCTTTCTCATTATCTCTGGTAAATGGATATAATTTTTAGCATAAGGTCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACacaaca   <  1:359030/150‑1 (MQ=255)
                                                                                                                                                       agagGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAacac  <  1:1083634/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGC‑‑AAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACAC  >  NC_000913/2900178‑2900476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: