Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,931,836 T→G 6.0% intergenic (+26/+29) ygdG → / ← fucO Ssb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,8360TG 6.0% 247.8 / 3.2 99intergenic (+26/+29)ygdG/fucOSsb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (47/45);  new base G (3/4);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTA  >  NC_000913/2931687‑2931983
                                                                                                                                                     |                                                                                                                                                     
cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                       >  1:247997/1‑150 (MQ=255)
   aaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcgt                                                                                                                                                    <  1:122540/150‑1 (MQ=255)
     aGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTACCGGCGCGCCGGATACGCCGCAAACGTCGTAt                                                                                                                                                  >  2:77766/1‑150 (MQ=255)
          cgcAAAAATTTAGAAACCCATACAGAGCTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGc                                                                                                                                             <  1:693303/150‑1 (MQ=255)
          cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGc                                                                                                                                             <  2:247997/150‑1 (MQ=255)
          cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGc                                                                                                                                             <  1:474480/150‑1 (MQ=255)
          cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGc                                                                                                                                             <  1:147234/150‑1 (MQ=255)
                     aGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATAAGCCGCAAAAGTCGTATCCGGGCTTATAACATc                                                                                                                                 >  2:1796281/1‑150 (MQ=255)
                      tataCCGATAAAGAGATGGAGTTTCTGTGCCGCGATATTGGCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCATACGTCGTATCCGGCTTTATCACATCa                                                                                                                                <  1:928686/147‑1 (MQ=255)
                      gAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACCTcc                                                                                                                                >  2:1423853/1‑149 (MQ=255)
                      gAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCa                                                                                                                                >  1:1069935/1‑150 (MQ=255)
                      gAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACACCa                                                                                                                                <  2:693836/150‑1 (MQ=255)
                        aaCCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTTTACGGCATTTTGCCATCAgc                                                                                                                              >  2:641494/1‑150 (MQ=255)
                        aaCCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAgc                                                                                                                              >  1:1183580/1‑150 (MQ=255)
                          cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACTTCGTATCCGGCATTATCACATCAgcgc                                                                                                                            >  1:1296731/1‑150 (MQ=255)
                          cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAgcgc                                                                                                                            >  2:1033391/1‑150 (MQ=255)
                          cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTACCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTTGTATCCGGCATTATCACATCAgcgc                                                                                                                            >  1:1360367/1‑150 (MQ=255)
                           ccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGCTAAGCCGCCAACCTCGTATGCGGCGTTTTGACGACAgcgcc                                                                                                                           >  2:1856502/1‑149 (MQ=255)
                            cATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAt                                                                                                                         >  2:1106787/1‑150 (MQ=255)
                             aTAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCCAACCGCGTGTCGGGGCGTTTCACATCACCGCCtt                                                                                                                        >  1:690189/1‑150 (MQ=255)
                              tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGCGCCGGATACGCCGCAAACGTCGTCTGCGGCATTATCACATCAGCGCAttt                                                                                                                       >  1:91606/1‑150 (MQ=255)
                              tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                       <  1:706717/150‑1 (MQ=255)
                               aaaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGACGGATACGCCGCAATAGTCGTATCCGGCATTATGACATAAGCGCATTTa                                                                                                                      >  1:217402/1‑150 (MQ=255)
                                aaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATACCGCATTATCACAACAGCGCATTTAc                                                                                                                     >  1:143997/1‑150 (MQ=255)
                                aaGAGATGGCGTTTCTGTGCCGCGATATTCCCCGCTTACAAACCGATTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAcc                                                                                                                    <  1:1574801/150‑1 (MQ=255)
                                       ggCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCgg                                                                                                              <  1:476427/150‑1 (MQ=255)
                                        gCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGTTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGt                                                                                                             <  1:1531925/150‑1 (MQ=255)
                                         cGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTa                                                                                                            <  1:664695/150‑1 (MQ=255)
                                         cGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGCATAAACCCCAAACGTCGTATACGGCCTTATAACATAAGCGCATTTACCAGGCCGTa                                                                                                            >  2:2000780/1‑150 (MQ=255)
                                            ttCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACCCCGCAAACGGCGGATGCGGGAATATTAAAAGAGCGCAATTTACCCGgggggtgg                                                                                                          >  1:1727094/1‑146 (MQ=255)
                                             tCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGt                                                                                                        <  1:713957/150‑1 (MQ=255)
                                              cTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTa                                                                                                       >  1:1352448/1‑150 (MQ=255)
                                              cTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTCGTACGGTAACGGCGAGCCCGATTCGCCGCAAAAGTCGTATACGGCATTATCACATCAGCCCAATTACCACGCGGTATGGTa                                                                                                       >  2:1915399/1‑150 (MQ=255)
                                                gtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACCTAGTATCCGGCAATATAACCTCAGCGCATTTACCCGGCGGGATGGGaaa                                                                                                     >  1:1254709/1‑150 (MQ=255)
                                                 tgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGGTACGCCGCCAACCTCGGTTACGGGATTATGACATAACCGCATTTAACAGGGGGTATGGTatac                                                                                                    >  1:1773115/1‑147 (MQ=255)
                                                  gCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCCAACGTCGGATCCGGCATTTTAGACACAGCGCAGTTTCCCGGCGGGATGGTTAAGc                                                                                                   >  1:791551/1‑150 (MQ=255)
                                                  gCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACCTCCGCGCATTTACCCGGCGGTATGGTAAAGc                                                                                                   >  2:1376374/1‑150 (MQ=255)
                                                  gCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGc                                                                                                   >  1:1151797/1‑150 (MQ=255)
                                                   ccGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATCCGGCATTATCACAAAAGCGCATTTACCACGCGGTATTGTAAAGct                                                                                                  >  1:156445/1‑150 (MQ=255)
                                                       gATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATACGGCATTATGACATCAGCGCCTTTACCACGCGGTATGGTTAAGCTCTAc                                                                                              >  2:379450/1‑150 (MQ=255)
                                                              cccGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATcc                                                                                       <  1:1608096/150‑1 (MQ=255)
                                                                  cTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGCTTAGGCCCAAACGTACTCTCCGGCATTATACCATCAGCGCCTTTACCACGCGGGATTGTAAATCTCTAAAataaaaaaaa                                                                                   >  2:618896/1‑143 (MQ=255)
                                                                    tACTAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGGATCCGGCATTATCACTTCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGc                                                                                 <  2:1046076/150‑1 (MQ=255)
                                                                     aCAACCCGATTTGCATATCGACGGCAATTTACAGCAAATGCGGTTTGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCTCATTTACCAGGCGGTATGGTAAATCTCTGCAATATCCTCAAGCg                                                                                <  2:1477270/150‑1 (MQ=255)
                                                                      cAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                               <  1:774672/150‑1 (MQ=255)
                                                                       aaaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGtt                                                                              <  2:943192/150‑1 (MQ=255)
                                                                       aaaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGACGGATACGCCGCAAACGTCGTATCAGGCAGTATCACATCAGCGCATTTACCAGGCCGTATGGTAAAGCTATACAATATCCTCAAGCGtg                                                                              >  1:272400/1‑149 (MQ=255)
                                                                        aaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTg                                                                             <  2:1739346/150‑1 (MQ=255)
                                                                        aaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATAAGCCGCAAACGTCGTATACGGGATTTTAACATAAGCGCATTTACCACGCGGGATGGTAAAGCTAGAAAATATCCACAAGCGGTg                                                                             >  2:815546/1‑150 (MQ=255)
                                                                         aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGc                                                                            <  1:1033391/150‑1 (MQ=255)
                                                                           cGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCtt                                                                          <  2:1770855/150‑1 (MQ=255)
                                                                             aTTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATTAGCCGCCAACGTCGTATCCGGCATTATCACATCAGCGCCTTTTACAGGCGGTATGGTAAATCTCTACAATATCCTCAAGCGTTGCttac                                                                        >  1:546240/1‑148 (MQ=255)
                                                                             aTTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTATAGCTCTACAATATACTCAAGCGTTGCTTcc                                                                        >  1:44384/1‑149 (MQ=255)
                                                                                tGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATAACATAAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAAAATACTAAAGCGTTGCTTCAcgc                                                                     >  2:1836929/1‑150 (MQ=255)
                                                                                tGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTAGTATACGGCATTATCAAAACACCGCATTTACCCGGCGGTATGGTAAAGCGCTACAAAATCCAAAAGCGATGcataccgc                                                                     >  2:967620/1‑143 (MQ=255)
                                                                                     atCGACGGCAATTTACAGCAATTGCGGTTTGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGtt                                                                <  2:1344632/150‑1 (MQ=255)
                                                                                      tCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTg                                                               <  2:1158695/150‑1 (MQ=255)
                                                                                      tCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTg                                                               <  2:1181224/150‑1 (MQ=255)
                                                                                         aCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCa                                                            <  2:1171262/150‑1 (MQ=255)
                                                                                         aCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCa                                                            <  1:1106787/150‑1 (MQ=255)
                                                                                             cAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATCCGGCATTATCACATAAGCGCATTTACCAGGCGGGATGGTAAAGCTCTACAATATACTCAAGCGTTGCTTCACGCGGGTTGCCCCCgg                                                        >  1:1892299/1‑150 (MQ=255)
                                                                                              aaTTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTAACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGt                                                       <  2:1088146/150‑1 (MQ=255)
                                                                                              aaTTTACAGCAATTGCGGTGGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGTTTGCCACCGGt                                                       <  1:167203/150‑1 (MQ=255)
                                                                                                  tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGCTACGCCGCAAACGTCGTATCCGGGATTTTAACCTCAGCGCATTTACCCGGCGGTATGGTAAAGCTCTACAATATACACAAGCGTTGCTTCACGCGGGTTGCCACCCGGACaa                                                   >  1:1913126/1‑150 (MQ=255)
                                                                                                  tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACaa                                                   >  2:1026957/1‑150 (MQ=255)
                                                                                                    cAGCAATTGCGGTTTGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAc                                                 <  2:1097911/150‑1 (MQ=255)
                                                                                                    cAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAc                                                 <  2:1991060/150‑1 (MQ=255)
                                                                                                     aGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAca                                                <  2:1061783/150‑1 (MQ=255)
                                                                                                      gCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCACGCGTTGGTTCACGCGGGTTGCCACCGGGACAAAcaa                                               >  2:826067/1‑149 (MQ=255)
                                                                                                      gCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATAACCCGCAAACGTAGTATACGGCATTATAACCAAAGCGCAATTTGCAGGCGGTATGGGTAAACGGTAAAAAAAACACAAACGTTTGTTAACGCGGGTTTGCACCGGTACAAAcaa                                               >  2:1556571/1‑149 (MQ=255)
                                                                                                             cGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAg                                        <  2:896313/150‑1 (MQ=255)
                                                                                                                ttGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgc                                     <  2:1058654/150‑1 (MQ=255)
                                                                                                                     aCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCt                                <  1:1780379/150‑1 (MQ=255)
                                                                                                                       ggTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTgc                              <  2:1042052/150‑1 (MQ=255)
                                                                                                                            cGGCGAGCCGGATACGCCGCAATAGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGTTACAAACATCATCCAGTGCCGCCTGCGCCAg                         <  2:515335/150‑1 (MQ=255)
                                                                                                                            cGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAg                         <  1:1638061/150‑1 (MQ=255)
                                                                                                                             ggCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGt                        >  2:1322423/1‑150 (MQ=255)
                                                                                                                               cGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGc                      >  2:466362/1‑150 (MQ=255)
                                                                                                                                gAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGcc                     >  1:851964/1‑150 (MQ=255)
                                                                                                                                  gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCTGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg                   <  2:143997/150‑1 (MQ=255)
                                                                                                                                  gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAATGCTCTACAATATCCTCAAGAGTTGCTTCACGCGGGTTGCCCCCGGTACATACTTCATCCAGTGCCGCCTGCGCCAGTGCCgt                   >  2:449091/1‑149 (MQ=255)
                                                                                                                                   ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                  >  1:1611546/1‑150 (MQ=255)
                                                                                                                                    cGGATACGCCGCAAAAGTCGTATCCGGCATTATCACATCAGCGCTTTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAAAATCATCCAGTGCCGCCTGCGCCAGTGCCGGaa                 <  2:432213/150‑1 (MQ=255)
                                                                                                                                     ggATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACCTCATCCAGTGCCGCCTGCGCCAGTGCCGGAAt                >  2:810987/1‑150 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg               <  2:1254709/150‑1 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg               <  2:1730938/150‑1 (MQ=255)
                                                                                                                                      gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg               <  1:1486535/150‑1 (MQ=255)
                                                                                                                                        tACGGCGCAAACGGCGGTTCCGGCATTTTGACTTGAGCGGATTTACCATGCGGTATGGTAAAGCTCTACAATATCCTCAAGCCTTGCTTCACGCGGGTTGCCAACGGTACAAACATCATCCATTGCCGCCTTCGCCAGTGGCGGAATGTc             <  2:488958/150‑1 (MQ=255)
                                                                                                                                        tACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTc             <  2:135943/150‑1 (MQ=255)
                                                                                                                                         aCGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTGCCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCt            <  1:1556571/150‑1 (MQ=255)
                                                                                                                                         aCGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCt            <  2:574676/150‑1 (MQ=255)
                                                                                                                                             cgcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCt        >  1:214352/1‑150 (MQ=255)
                                                                                                                                              gcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGATTGTCTTCCtt       >  1:1238564/1‑150 (MQ=255)
                                                                                                                                               cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGATTGTCTTCCTTg      >  1:1226552/1‑150 (MQ=255)
                                                                                                                                               cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg      >  1:722782/1‑150 (MQ=255)
                                                                                                                                                aaaCGTCGTATCCGGCATTATCACATCAGCGGATTATCGAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGc     <  1:1396467/150‑1 (MQ=255)
                                                                                                                                                aaaCGTCGTATCCGGCATTATCACATCAGCGCATTTACCCGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCCCCGGTACAAACATCATCCAGTGCCGCATGCGCCAGTGCCGGAATGTCTTCCTTGc     >  2:1068752/1‑150 (MQ=255)
                                                                                                                                                  aCGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGt   <  1:1360577/150‑1 (MQ=255)
                                                                                                                                                  aCGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGt   <  2:156445/150‑1 (MQ=255)
                                                                                                                                                   cgtcgtATCCGGCATTATCACATCAGCGCATTTAGCAGGCGGTATGGTAAAGCTCTACAAAATCCTCAAGCGTTGCTTCCCGCGGGTTGCCAGCGGTACCAACATCATCCACTGCCGCCTGCTCCAGTGCCGGAATGTCTTCCTTGCGTa  >  1:101736/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCGTA  >  NC_000913/2931687‑2931983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: