Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,146 T→C 5.8% intergenic (+13/‑125) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1460TC 5.8% 355.4 / 9.3 120intergenic (+13/‑125)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (46/66);  new base C (4/3);  total (50/70)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 121 total aligned reads displayed.

GTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATT  >  NC_000913/2971005‑2971294
                                                                                                                                             |                                                                                                                                                    
gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                              >  2:1281904/1‑150 (MQ=255)
 ggggCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                                                             <  2:1659855/149‑1 (MQ=255)
  gggCTTTACCCTGATTATGGAACGTTTCTACATCCACGTACCGAAAGGATACATCTAAATCGCGATGTTCTTCTCTATTGAGGGTCAAAGCCTCTAGATGTTTCGCATCAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCggg                                                                                                                                            <  2:1551444/150‑1 (MQ=255)
    gCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACTTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCAGCTCTGATACTCCGTACGCTCTCCTGCGGGAg                                                                                                                                          >  1:919566/1‑150 (MQ=255)
      tttACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                                                                        <  1:1104159/150‑1 (MQ=255)
        tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGTTTACATCTACTTCGCGATGTTCTTCTCTTTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                      <  1:453149/150‑1 (MQ=255)
          cccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                                                                    >  1:1653945/1‑150 (MQ=255)
           ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                                                                   >  1:1042306/1‑150 (MQ=255)
           ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                                                                   <  1:1156159/150‑1 (MQ=255)
            cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                                                                  <  1:6249/150‑1 (MQ=255)
            cTGATTCTGGAAAGTTGCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGGTGAAAGCCGCAACTTGATTCGCAACAAAAAGAATCCGCGCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                                                                  <  2:687586/150‑2 (MQ=255)
              gATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGTTGTTCTTCTCTATTGCGGTTGAAAGCGTCAACTTGATTCGCAACAAAAAGAATCCGCTATGATACTCCGTACGCTCTCATGCGGGAGGGCGTactca                                                                                                                                >  1:1351626/1‑150 (MQ=255)
              gATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                                                                                <  2:936472/150‑4 (MQ=255)
               aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                                                               <  2:81408/150‑1 (MQ=255)
                 tCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcactc                                                                                                                             >  1:431145/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                            >  2:1820027/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                            >  1:38955/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCATACTCACTcc                                                                                                                            >  1:1587273/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGGACCGAAAGGTTACATCTACTTCGCGCTGTTCTTCTCTATTGCGGTTGACAGCCTCAACTTGATTCGCACCAAACAGAATCCGCGCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                            <  1:777091/150‑1 (MQ=255)
                        aGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                                                                      <  1:1345729/150‑1 (MQ=255)
                          tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAGGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAccc                                                                                                                    <  2:615911/150‑1 (MQ=255)
                             cGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                 >  2:772608/1‑150 (MQ=255)
                              gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcac                                                                                                                >  2:904755/1‑150 (MQ=255)
                                cATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                              >  1:1215443/1‑150 (MQ=255)
                                cATCCACGTACCCAAAGGTTACATCTACTTCGCGATTTTCTTCTCTATTGCGGATGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTACGTACGCTCTCCGGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                              <  1:56062/150‑1 (MQ=255)
                                   ccACGTACCGAAAGTTTACATCTACTTCGAGATGTTCTTCTCTATTGCGTTTTAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                                                           <  1:1934280/150‑1 (MQ=255)
                                   ccACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                                                           >  2:1475226/1‑150 (MQ=255)
                                     aCGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                         <  1:1739247/150‑1 (MQ=255)
                                      cGTACCGAAAGGTTTCATCTACTTCGCGATGTTATTCTCTATTGCGGTTGAAAGCCGCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCGACGGCGGGAGGGCGGACTCACTCCTTTTTCCCTCACAGTTTaat                                                                                                        >  2:821029/1‑149 (MQ=255)
                                         aCCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                                                     <  1:990385/150‑1 (MQ=255)
                                           cGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                                                                   >  1:800201/1‑150 (MQ=255)
                                            gAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                  >  2:1961904/1‑150 (MQ=255)
                                            gAAAGGTTACATCTACTTCGCGATGATCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                  <  1:62451/150‑1 (MQ=255)
                                             aaaGGTTACATCTACTACGCGATGTTCTTCTCTAATGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATTCTCCGTAAGCTCTCCGGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                                                                 <  1:1017377/150‑1 (MQ=255)
                                               aGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGACTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                                                               <  1:101370/150‑1 (MQ=255)
                                               aGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                                                               <  2:1684903/150‑1 (MQ=255)
                                                ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTTCTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                              <  2:1384293/150‑1 (MQ=255)
                                                    aCATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTAAGCCCTCCTTTTACCCTCACAGTTTAAGAGTTTCCTGCTTTc                                                                                          >  1:156793/1‑150 (MQ=255)
                                                     cATCTACATCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAACAAGAATCCGCACTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                                                         <  2:159642/150‑1 (MQ=255)
                                                       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                       <  2:1620565/150‑1 (MQ=255)
                                                       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                       <  2:1947092/150‑1 (MQ=255)
                                                       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                       <  1:210918/150‑1 (MQ=255)
                                                        cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                      >  1:1572104/1‑150 (MQ=255)
                                                        cTACTTCGCCTTGTTCTTCTCTATTGCGGTTGAAAGCCTCTACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTGCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                      <  2:331622/150‑1 (MQ=255)
                                                         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                                                                     <  1:437646/150‑1 (MQ=255)
                                                          aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                                                    <  2:809305/150‑1 (MQ=255)
                                                          aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                                                    <  2:1505579/150‑1 (MQ=255)
                                                             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGttatat                                                                                 <  2:137966/150‑1 (MQ=255)
                                                             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatatat                                                                                 <  2:2000716/150‑1 (MQ=255)
                                                              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                                                                >  2:319115/1‑150 (MQ=255)
                                                              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                                                                <  1:680852/150‑1 (MQ=255)
                                                                 gATGTTCTTCTCTATTGCGGTTGAAAGCCCCAACTTGATTCGCAACAAAAAGACTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTACTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACg                                                                             <  2:381295/150‑1 (MQ=255)
                                                                    gTTCTTCTCTATTCCGGTTGAAAGCCTCAACTAGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                                                                          >  2:697189/1‑150 (MQ=255)
                                                                     ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCgg                                                                         <  1:1281904/150‑1 (MQ=255)
                                                                      tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                        <  2:238701/150‑1 (MQ=255)
                                                                       cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                                                       <  1:1362630/150‑1 (MQ=255)
                                                                        ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                      >  1:1890095/1‑150 (MQ=255)
                                                                        ttctCTATTGCGGTTGAAAGCCACAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                      >  1:1002629/1‑150 (MQ=255)
                                                                          ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                                                                    >  2:1683734/1‑150 (MQ=255)
                                                                            ctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                                                                  <  1:1465004/150‑1 (MQ=255)
                                                                               ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                                                               <  1:772371/150‑1 (MQ=255)
                                                                                 gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                                                             >  1:436788/1‑150 (MQ=255)
                                                                                 gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACGCCTTTTACCCTCACAGTTTAAGATTTTCCTGCGTTCAAGATATATAACGTCGGTTTATAAACAGa                                                             >  1:1169269/1‑150 (MQ=255)
                                                                                     ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                                                         <  2:646111/150‑1 (MQ=255)
                                                                                      tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatt                                                        >  2:340667/1‑150 (MQ=255)
                                                                                         aaGCCTCAACTTGATTCTCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc                                                     <  2:834132/150‑1 (MQ=255)
                                                                                         aaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTAGGTTTATAAACAGACTATTATc                                                     >  1:1829667/1‑150 (MQ=255)
                                                                                          aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAAATATATAACGTCGGTTTATAAACAGACTATTATCa                                                    <  1:1678596/150‑1 (MQ=255)
                                                                                                  cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg                                            <  2:1446909/150‑1 (MQ=255)
                                                                                                   ttGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCCCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGc                                           >  2:1265409/1‑150 (MQ=255)
                                                                                                    tGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGcc                                          >  1:1019915/1‑150 (MQ=255)
                                                                                                     gATTCGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCa                                         <  1:1645386/150‑1 (MQ=255)
                                                                                                       ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCaaa                                       <  2:1052874/150‑1 (MQ=255)
                                                                                                           caacatAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGac                                   <  1:1421565/150‑2 (MQ=255)
                                                                                                            aacaaAAAGAATCCTCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTACTGCTTTAAAGATATATAACGTCGGTTTATAAACGGACTATTATCATAGGTAGGCCAAATGAgg                                  <  1:944365/150‑1 (MQ=255)
                                                                                                            aacaaAAAGAATCCGCGCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAgg                                  <  2:642506/150‑1 (MQ=255)
                                                                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGAt                                >  2:1698102/1‑150 (MQ=255)
                                                                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGAt                                >  1:1716633/1‑150 (MQ=255)
                                                                                                               aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                               <  2:876216/150‑1 (MQ=255)
                                                                                                               aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                               <  2:868578/150‑1 (MQ=255)
                                                                                                                 aaaGAATCCGCTCTGATACTCCGTATGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAAc                             >  2:1248950/1‑150 (MQ=255)
                                                                                                                  aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAAcc                            <  2:1947526/150‑1 (MQ=255)
                                                                                                                  aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAAcc                            >  2:1077466/1‑150 (MQ=255)
                                                                                                                    gAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTAAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCgg                          <  1:772695/150‑1 (MQ=255)
                                                                                                                      aTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAAATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGAt                        <  2:1106596/150‑1 (MQ=255)
                                                                                                                      aTCCGATCAGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTATGATTATCCTGCTTTCAAGATATATAACGTCGGTTTATAACCAGACTATTATCATATGTAGGCCAAATGAGGATAACCGGAt                        <  2:702904/150‑1 (MQ=255)
                                                                                                                           ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                   <  1:904755/150‑1 (MQ=255)
                                                                                                                             ctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaaaa                 >  2:931004/1‑150 (MQ=255)
                                                                                                                               gATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAATATATATAACGTCGGTTTATAAACAGACTATTATCATCGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg               <  1:969683/150‑1 (MQ=255)
                                                                                                                                 tACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCTTAGGTAGGCCAAATGAGGATAACCGGTTGAAAAAATggg             <  1:697189/150‑1 (MQ=255)
                                                                                                                                  aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGc            <  2:1007065/150‑1 (MQ=255)
                                                                                                                                   cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCa           >  2:1064434/1‑150 (MQ=255)
                                                                                                                                    tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAg          >  2:905772/4‑150 (MQ=255)
                                                                                                                                    tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAg          >  1:221/1‑150 (MQ=255)
                                                                                                                                       gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTGCTGGTTTCAAGATATATAACGTCGGGTTATAAACATACTATTATCATAGGTAGGCGAATTGAGGTTAACCGGATGAAAAAATGGGCAGtaa       >  1:1236516/1‑150 (MQ=255)
                                                                                                                                          cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAATTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaataa    <  2:1550465/150‑1 (MQ=255)
                                                                                                                                          cGCTCTCCAGCGGGAGGGCGTACTAACTCCTTTTACCCTCACAGTATAAGATTTTCCTGCTTTCAAGATATATAACGACGGTTTATAAACAGACTATTATCATAGGTAGGCCTAATGAGGATAACCGGAGGAATAAATCGGCAGtaataa    >  2:1736877/1‑150 (MQ=255)
                                                                                                                                          cGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTTTAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaataa    >  2:510475/1‑150 (MQ=255)
                                                                                                                                           gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTGAGATTTTCCTGCTTTCAAGATATATTACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat   <  2:1490033/150‑1 (MQ=255)
                                                                                                                                            ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACTGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAAtt  >  2:508697/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATT  >  NC_000913/2971005‑2971294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: