Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,154 G→A 5.7% intergenic (+21/‑117) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1540GA 5.7% 377.4 / 6.7 123intergenic (+21/‑117)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (48/67);  new base A (4/3);  total (53/70)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 127 total aligned reads displayed.

GTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGT  >  NC_000913/2971005‑2971302
                                                                                                                                                     |                                                                                                                                                    
gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                                      <  1:316653/150‑1 (MQ=255)
gtggGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                                      >  2:1281904/1‑150 (MQ=255)
 ggggCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                                                                     <  2:1659855/149‑1 (MQ=255)
  gggCTTTACCCTGATTATGGAACGTTTCTACATCCACGTACCGAAAGGATACATCTAAATCGCGATGTTCTTCTCTATTGAGGGTCAAAGCCTCTAGATGTTTCGCATCAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCggg                                                                                                                                                    <  2:1551444/150‑1 (MQ=255)
    gCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACTTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCAGCTCTGATACTCCGTACGCTCTCCTGCGGGAg                                                                                                                                                  >  1:919566/1‑150 (MQ=255)
      tttACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                                                                                <  1:1104159/150‑1 (MQ=255)
      tttACACTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGAGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCTGGAggg                                                                                                                                                >  2:1521433/1‑150 (MQ=255)
        tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGTTTACATCTACTTCGCGATGTTCTTCTCTTTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                                              <  1:453149/150‑1 (MQ=255)
          cccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                                                                            >  1:1653945/1‑150 (MQ=255)
           ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTac                                                                                                                                           <  1:1156159/150‑1 (MQ=255)
            cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                                                                          <  1:6249/150‑1 (MQ=255)
            cTGATTCTGGAAAGTTGCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGGTGAAAGCCGCAACTTGATTCGCAACAAAAAGAATCCGCGCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                                                                          <  2:687586/150‑2 (MQ=255)
              gATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGTTGTTCTTCTCTATTGCGGTTGAAAGCGTCAACTTGATTCGCAACAAAAAGAATCCGCTATGATACTCCGTACGCTCTCATGCGGGAGGGCGTactca                                                                                                                                        >  1:1351626/1‑150 (MQ=255)
              gATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgga                                                                                                                                        <  2:936472/150‑4 (MQ=255)
               aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                                                                       <  2:81408/150‑1 (MQ=255)
                 tCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcactc                                                                                                                                     >  1:431145/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                                    >  2:1820027/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                                    >  1:38955/1‑150 (MQ=255)
                  cTGGAAAGTTTCGACATCCACGGACCGAAAGGTTACATCTACTTCGCGCTGTTCTTCTCTATTGCGGTTGACAGCCTCAACTTGATTCGCACCAAACAGAATCCGCGCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                                    <  1:777091/150‑1 (MQ=255)
                   tGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                                   >  2:1678596/1‑150 (MQ=255)
                       aaGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTa                                                                                                                               <  2:67546/150‑1 (MQ=255)
                          tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAGGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAccc                                                                                                                            <  2:615911/150‑1 (MQ=255)
                             cGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                                         >  2:772608/1‑150 (MQ=255)
                                cATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                                      >  1:1215443/1‑150 (MQ=255)
                                cATCCACGTACCCAAAGGTTACATCTACTTCGCGATTTTCTTCTCTATTGCGGATGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTACGTACGCTCTCCGGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                                                                      <  1:56062/150‑1 (MQ=255)
                                   ccACGTACCGAAAGTTTACATCTACTTCGAGATGTTCTTCTCTATTGCGTTTTAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                                                                   <  1:1934280/150‑1 (MQ=255)
                                   ccACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                                                                   >  2:1475226/1‑150 (MQ=255)
                                     aCGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                                 <  1:1739247/150‑1 (MQ=255)
                                           cGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                                                                           >  1:800201/1‑150 (MQ=255)
                                            gAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                                          >  2:1961904/1‑150 (MQ=255)
                                            gAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAGTTTc                                                                                                          >  1:1085130/1‑150 (MQ=255)
                                             aaaGGTTACATCTACTACGCGATGTTCTTCTCTAATGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATTCTCCGTAAGCTCTCCGGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                                                                         <  1:1017377/150‑1 (MQ=255)
                                              aaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCt                                                                                                        <  2:1874833/150‑1 (MQ=255)
                                               aGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGACTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                                                                       <  1:101370/150‑1 (MQ=255)
                                               aGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                                                                       <  2:1684903/150‑1 (MQ=255)
                                                ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTTCTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                                      <  2:1384293/150‑1 (MQ=255)
                                                   tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCttt                                                                                                   <  2:812810/150‑1 (MQ=255)
                                                    aCATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTAAGCCCTCCTTTTACCCTCACAGTTTAAGAGTTTCCTGCTTTc                                                                                                  >  1:156793/1‑150 (MQ=255)
                                                     cATCTACATCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAACAAGAATCCGCACTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                                                                 <  2:159642/150‑1 (MQ=255)
                                                       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                               <  1:210918/150‑1 (MQ=255)
                                                       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAg                                                                                               <  2:1947092/150‑1 (MQ=255)
                                                        cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                              >  1:1572104/1‑150 (MQ=255)
                                                        cTACTTCGCCTTGTTCTTCTCTATTGCGGTTGAAAGCCTCTACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTGCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                                              <  2:331622/150‑1 (MQ=255)
                                                         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                                                                             <  1:437646/150‑1 (MQ=255)
                                                          aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                                                            <  2:809305/150‑1 (MQ=255)
                                                             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGttatat                                                                                         <  2:137966/150‑1 (MQ=255)
                                                             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatatat                                                                                         <  2:2000716/150‑1 (MQ=255)
                                                              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                                                                        >  2:319115/1‑150 (MQ=255)
                                                              cgcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAtatata                                                                                        <  1:680852/150‑1 (MQ=255)
                                                                 gATGTTCTTCTCTATTGCGGTTGAAAGCCCCAACTTGATTCGCAACAAAAAGACTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTACTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACg                                                                                     <  2:381295/150‑1 (MQ=255)
                                                                    gTTCTTCTCTATTCCGGTTGAAAGCCTCAACTAGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCg                                                                                  >  2:697189/1‑150 (MQ=255)
                                                                     ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCgg                                                                                 <  1:1281904/150‑1 (MQ=255)
                                                                      tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                                                <  2:238701/150‑1 (MQ=255)
                                                                       cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                                                               <  1:1362630/150‑1 (MQ=255)
                                                                        ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                              >  1:1890095/1‑150 (MQ=255)
                                                                        ttctCTATTGCGGTTGAAAGCCACAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                                              >  1:1002629/1‑150 (MQ=255)
                                                                          ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtat                                                                            >  2:1683734/1‑150 (MQ=255)
                                                                            ctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                                                                          <  1:1465004/150‑1 (MQ=255)
                                                                               ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                                                                       <  1:772371/150‑1 (MQ=255)
                                                                                      tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatt                                                                >  2:340667/1‑150 (MQ=255)
                                                                                        aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat                                                              >  2:1203280/1‑150 (MQ=255)
                                                                                         aaGCCTCAACTTGATTCTCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc                                                             <  2:834132/150‑1 (MQ=255)
                                                                                             cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtag                                                         <  2:1004081/150‑1 (MQ=255)
                                                                                                  cTTGATTCGCAACAAAAAGAATCCTCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg                                                    <  1:1767896/150‑1 (MQ=255)
                                                                                                  cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg                                                    <  2:1446909/150‑1 (MQ=255)
                                                                                                   ttGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCCCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGc                                                   >  2:1265409/1‑150 (MQ=255)
                                                                                                    tGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGcc                                                  >  1:1019915/1‑150 (MQ=255)
                                                                                                     gATTCGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCa                                                 <  1:1645386/150‑1 (MQ=255)
                                                                                                           caacatAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGac                                           <  1:1421565/150‑2 (MQ=255)
                                                                                                           caacaaATAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTATTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAg                                           <  1:1652022/150‑1 (MQ=255)
                                                                                                            aacaaAAAGAATCCTCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTACTGCTTTAAAGATATATAACGTCGGTTTATAAACGGACTATTATCATAGGTAGGCCAAATGAgg                                          <  1:944365/150‑1 (MQ=255)
                                                                                                            aacaaAAAGAATCCGCGCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAgg                                          <  2:642506/150‑1 (MQ=255)
                                                                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGAt                                        >  1:1716633/1‑150 (MQ=255)
                                                                                                               aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                                       <  2:868578/150‑1 (MQ=255)
                                                                                                               aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                                       <  2:876216/150‑1 (MQ=255)
                                                                                                                  aaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAAcc                                    >  2:1077466/1‑150 (MQ=255)
                                                                                                                    gAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTAAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCgg                                  <  1:772695/150‑1 (MQ=255)
                                                                                                                     aaTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGa                                 <  2:896447/150‑1 (MQ=255)
                                                                                                                      aTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAAATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGAt                                <  2:1106596/150‑1 (MQ=255)
                                                                                                                           ctctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa                           <  1:904755/150‑1 (MQ=255)
                                                                                                                             ctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaaaa                         >  2:931004/1‑150 (MQ=255)
                                                                                                                               gATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAATATATATAACGTCGGTTTATAAACAGACTATTATCATCGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg                       <  1:969683/150‑1 (MQ=255)
                                                                                                                                ggagtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATgg                      >  1:511111/8‑150 (MQ=255)
                                                                                                                                 tACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCTTAGGTAGGCCAAATGAGGATAACCGGTTGAAAAAATggg                     <  1:697189/150‑1 (MQ=255)
                                                                                                                                  aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGc                    <  2:1007065/150‑1 (MQ=255)
                                                                                                                                   cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCa                   >  2:1064434/1‑150 (MQ=255)
                                                                                                                                    tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAg                  >  2:905772/4‑150 (MQ=255)
                                                                                                                                       gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTGCTGGTTTCAAGATATATAACGTCGGGTTATAAACATACTATTATCATAGGTAGGCGAATTGAGGTTAACCGGATGAAAAAATGGGCAGtaa               >  1:1236516/1‑150 (MQ=255)
                                                                                                                                         aCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTATCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGACAAATGAGGATAACCGGATGAAAAAATGGGCAGtaata             >  1:1260614/1‑150 (MQ=255)
                                                                                                                                          cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAATTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaataa            <  2:1550465/150‑1 (MQ=255)
                                                                                                                                          cGCTCTCCAGCGGGAGGGCGTACTAACTCCTTTTACCCTCACAGTATAAGATTTTCCTGCTTTCAAGATATATAACGACGGTTTATAAACAGACTATTATCATAGGTAGGCCTAATGAGGATAACCGGAGGAATAAATCGGCAGtaataa            >  2:1736877/1‑150 (MQ=255)
                                                                                                                                          cGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTTTAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGtaataa            >  2:510475/1‑150 (MQ=255)
                                                                                                                                           gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTGAGATTTTCCTGCTTTCAAGATATATTACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTaataat           <  2:1490033/150‑1 (MQ=255)
                                                                                                                                            ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACTGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAAtt          >  2:508697/1‑150 (MQ=255)
                                                                                                                                              ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTc        >  1:1629284/1‑150 (MQ=255)
                                                                                                                                                ccTGCGGGTGGGCGTAATCACTCCTTTTACACTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTg      <  1:1066076/150‑1 (MQ=255)
                                                                                                                                                 cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGc     <  1:288855/150‑1 (MQ=255)
                                                                                                                                                  tGCGGGAGGGCGTACTGACTCCTTTTACCCTCAGAGTTTAAGATATTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCATATGAGGATTACCGGATGAAAAAATGGGCAGTAATAATTTCTGCa    >  1:215065/1‑150 (MQ=255)
                                                                                                                                                  tGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAACTGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCa    <  1:1458289/150‑1 (MQ=255)
                                                                                                                                                    cGGGATGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATAAAACGTCGGTTTATAAACATACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGt  >  2:1052671/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGT  >  NC_000913/2971005‑2971302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: