Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,320 T→G 5.6% noncoding (66/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3200TG 5.6% 334.4 / 4.8 117noncoding (66/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (58/51);  new base G (4/3);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 117 total aligned reads displayed.

CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976172‑2976467
                                                                                                                                                      |                                                                                                                                                   
ccAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATc                                                                                                                                                    >  1:491355/1‑150 (MQ=255)
   atcaacCCCTCTGGGAGCTTGATTGTGGTCTGCACGACGACTCTGCGCCAGCGAGAAAACGCAAAGGAATGAAGGTAAATTCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGTCAAAAAAAACCTGCGCAGCTgc                                                                                                                                                 <  1:81399/150‑1 (MQ=255)
    taaatACCACTGGTATATTGAAGGTTGACTGCACGACGAATCTTCCCCAGCGAGAAAACGCAAATGAAACAAGGGAAATGCCCCGAGGTGTGTATATACTCTGCTACTGTTACAGAAAGATGACCGGCAAAAAAAACCTGCGCATCTgcg                                                                                                                                                <  2:783782/148‑1 (MQ=255)
     aaatACCTCTGGTAAATTGAATGTGGTCTGCACGAAGACTCTTTGCCAGCGAGTATACGCAAATGAATGATCGGATATGGTTCGAGTTGTGTATATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcgc                                                                                                                                               <  2:314863/149‑1 (MQ=255)
      aatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCTCCAGCTAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                              <  1:1292928/150‑1 (MQ=255)
         aCCTCGGGGATCTTGATTGTGGTCGGCACGACGACTCTTCGCCCGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGc                                                                                                                                           <  1:1298271/150‑1 (MQ=255)
          ccTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                                                                          >  1:1642275/1‑150 (MQ=255)
           ctctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                         <  2:1496168/150‑1 (MQ=255)
           ctctGGGATCTTGATTGTGGGCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                         <  2:1711155/150‑1 (MQ=255)
            tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                        >  2:671395/1‑150 (MQ=255)
            tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                        <  1:1437256/150‑1 (MQ=255)
             ctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGCTGTGTAAATTGTCGGTTACTGTTACAGTTTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCCGGCTGgt                                                                                                                                       >  2:201464/1‑150 (MQ=255)
              tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                      <  1:489824/150‑1 (MQ=255)
              tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                      <  1:1851916/150‑1 (MQ=255)
              tGGGATCTTGATAGTGGTCTGCACGACGCCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                      <  1:803495/150‑1 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                     >  2:1180240/1‑150 (MQ=255)
                 gATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTat                                                                                                                                   >  1:419287/1‑149 (MQ=255)
                  aTCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAt                                                                                                                                  >  1:658852/1‑150 (MQ=255)
                  aTCTTGATTGTGGTCTGAACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAt                                                                                                                                  <  1:1647972/150‑1 (MQ=255)
                    attGATTGTGGTCTGCACGACGACTCTTCTCCAGCGAGAAAACGCAAAGTAATTAAGGGAAATGCAAAGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAATACCTGCGAATCTGCGCAGGCTGGTGTAAtta                                                                                                                                <  1:579832/149‑2 (MQ=255)
                     ttGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCa                                                                                                                               >  1:758905/1‑150 (MQ=255)
                        aTTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgt                                                                                                                            >  2:168892/1‑150 (MQ=255)
                         ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                           <  1:1068031/150‑1 (MQ=255)
                          tgtgGTCTGCACTACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                                                                          <  1:420126/150‑1 (MQ=255)
                          tgtgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                                                                          <  1:1951198/150‑1 (MQ=255)
                           gtgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGc                                                                                                                          <  2:1565235/150‑1 (MQ=255)
                            tgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTc                                                                                                                        <  2:1100393/150‑1 (MQ=255)
                                cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                    <  1:1251888/150‑1 (MQ=255)
                                 tGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAccc                                                                                                                   >  1:1342783/1‑150 (MQ=255)
                                  gCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTTTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCg                                                                                                                  >  1:570600/1‑150 (MQ=255)
                                  gCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGGTGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCg                                                                                                                  >  1:397038/1‑150 (MQ=255)
                                   cACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGa                                                                                                                 <  1:1870379/150‑1 (MQ=255)
                                     cgccgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCGGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAg                                                                                                               >  2:309092/4‑150 (MQ=255)
                                     cgacgcCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACGGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCCTGTGCTCAACCCGAAg                                                                                                               >  2:620688/1‑150 (MQ=255)
                                     cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAg                                                                                                               <  1:1915690/150‑1 (MQ=255)
                                     cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAg                                                                                                               >  2:1720966/1‑150 (MQ=255)
                                     cgacgaCTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAg                                                                                                               <  1:1001313/150‑1 (MQ=255)
                                      gacgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGt                                                                                                              >  1:394391/1‑150 (MQ=255)
                                      gacgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGt                                                                                                              >  1:705767/1‑150 (MQ=255)
                                        cgacTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAATGGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTg                                                                                                            <  1:103185/150‑1 (MQ=255)
                                          acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                          >  2:1926060/1‑150 (MQ=255)
                                            tctTCGCCAGCTAGAAAACGCAAAGGAAGGATGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGGTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACtt                                                                                                        <  2:36806/150‑1 (MQ=255)
                                                cGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                                                                    <  1:520629/150‑1 (MQ=255)
                                                 cccAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGGGTAAATTGGCGGTGACTGTTACAGATTGATGACCTGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                   <  2:949785/149‑1 (MQ=255)
                                                    aGCGAGAAAACGCAAGGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGAACGGCAAAAAAAACCTCCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCAGAAGTTCACTTCACATATc                                                                                                >  1:282616/1‑150 (MQ=255)
                                                    aGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATc                                                                                                >  1:1533519/1‑150 (MQ=255)
                                                       gagaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTTATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAt                                                                                             <  2:1255113/150‑1 (MQ=255)
                                                         gaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTTCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGATCAACCAGAAGTTGACTTCACCTATCAATAc                                                                                           >  2:1770686/1‑150 (MQ=255)
                                                           aaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATTACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATACATGTGCTCAACCCTAAGTTGACTTCACCTATCAATACct                                                                                         >  2:332880/1‑150 (MQ=255)
                                                           aaaCGCAAAGGAATGAAGGGAAATGAAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACct                                                                                         <  1:1327832/150‑1 (MQ=255)
                                                             aCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACctct                                                                                       >  1:1889605/1‑150 (MQ=255)
                                                               gCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTgg                                                                                     <  2:1348889/150‑1 (MQ=255)
                                                                cAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                    >  1:1155459/1‑150 (MQ=255)
                                                                  aaGGAATGAAGTGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGAt                                                                                  <  1:137654/150‑1 (MQ=255)
                                                                      aaTGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATcacc                                                                              >  2:678521/1‑150 (MQ=255)
                                                                      aaTGAAGGGAAATGCAACGAGGGGTGTATATTGACGGGTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCGCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATcacc                                                                              <  1:1461826/150‑1 (MQ=255)
                                                                          aaGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGAGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCACTGCGATCACCACtt                                                                          >  2:1151767/1‑150 (MQ=255)
                                                                           aGGGAAATGTACCGTCGTGATTTAATTGTCGGTTACTGTAACATTTTGATTACCTTCAAAAAAAACCTGCGCTCCGGCGCATGCTTGTGTAATTCATGTTCTCCACCCGATTTTGACTTCACCTATCAATACCTCTGGGATCACCACttt                                                                         <  2:126682/150‑1 (MQ=255)
                                                                            gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTTTa                                                                        >  2:487274/1‑150 (MQ=255)
                                                                            gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTa                                                                        >  2:226763/1‑150 (MQ=255)
                                                                              gAAATGCAACGAGGAGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCTAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAACAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGc                                                                      >  1:358786/1‑150 (MQ=255)
                                                                               aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCa                                                                     <  1:397897/150‑1 (MQ=255)
                                                                                  tGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAcc                                                                  >  2:1659444/1‑150 (MQ=255)
                                                                                   gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                                                                 >  2:1330670/1‑150 (MQ=255)
                                                                                   gCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                                                                 >  1:1665904/1‑150 (MQ=255)
                                                                                     aaCGAGGTGTGTAAATTGTCGGTTACTGTTACATATTGATGACCTTCAAAAACAACCTGCGCATCTGCTCAGGCTGGTGTAATTCATGTGATCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGa                                                               <  2:559151/150‑1 (MQ=255)
                                                                                       cGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTATAGCATCCAGaac                                                             >  1:1610906/1‑149 (MQ=255)
                                                                                        gAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGTCAAAAAAAACCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTTCTAAACCCGATGTTTACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGc                                                            <  1:432696/150‑1 (MQ=255)
                                                                                           gtgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaa                                                         >  1:1323026/1‑150 (MQ=255)
                                                                                             gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCACCCTGAAGCCAAAc                                                       >  2:1143105/1‑150 (MQ=255)
                                                                                               ttAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTTCTCATCTGAGCAGGCTTGTGTAATTAATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAActc                                                     >  1:384063/2‑148 (MQ=255)
                                                                                                 aaaTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGcca                                                   >  1:1105722/1‑150 (MQ=255)
                                                                                                  aaTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGccac                                                  >  2:710215/1‑150 (MQ=255)
                                                                                                        cGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCgg                                            <  2:1374331/150‑1 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCgg                                            >  2:717906/1‑150 (MQ=255)
                                                                                                          gTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                          <  2:635755/150‑1 (MQ=255)
                                                                                                               tGTTAAAGATTGTTGACCTGCAAAAATAACATGCGCTTATGGTCTGGCTGGTGAAAGTGATGGTCGAAACCCGAATTTTACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                     <  2:1277617/150‑1 (MQ=255)
                                                                                                                gTTACAGATTTATTACCTGCAAAAAAAATCCTGCGCATCTGCTCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                     <  2:879030/150‑1 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    >  1:1483517/1‑150 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGc                                    >  1:603286/1‑150 (MQ=255)
                                                                                                                   aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAAc                                 >  1:543413/1‑150 (MQ=255)
                                                                                                                      gattgattACCGGCAAAAAAAACCTGCGCATCTTCGCAGGCTGGTCTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACATTAGCAACCTGAAGCCAAACGCCACCAGCGCTCAATCCCAACAGc                              >  2:406305/1‑150 (MQ=255)
                                                                                                                           atgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTc                         >  1:760211/1‑150 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGTATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                        <  2:398546/150‑1 (MQ=255)
                                                                                                                            tgaCCGGAAAAAATCACCTGAGCTGCTGATCTGGCTGGTGTAATTCATGTTCTAAACACGACGTTGACTTCACCTATCAATACCTCTGGTATCACCACTTTAGCAACCTGAAGCCAATCGCCACCAGCGGTCAATCGCAACAGCGTTTCg                        <  2:776101/150‑1 (MQ=255)
                                                                                                                             taCCGGCAAAACAAACCTGCGCATCTGCGCAGGCTGGTGTGATTCATGTTCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                       <  2:595667/149‑1 (MQ=255)
                                                                                                                             gaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                       <  2:397038/150‑1 (MQ=255)
                                                                                                                                cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAAGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCaaa                    >  2:1092102/1‑150 (MQ=255)
                                                                                                                                 ggCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTGTCGCAAAg                   >  2:1027409/1‑150 (MQ=255)
                                                                                                                                     aaaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAgtgt                <  1:1383742/150‑1 (MQ=255)
                                                                                                                                      aaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGCCTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCACCCGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTa               >  1:354159/1‑150 (MQ=255)
                                                                                                                                        aaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAc             <  1:1697485/150‑1 (MQ=255)
                                                                                                                                         aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAAACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGAAACAGCCTTTCGCAAAGTGTAAcc            >  1:1048068/1‑150 (MQ=255)
                                                                                                                                            agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCAACAGCGGTCAATCGCAAAAGCGTTTCGCAAAGTGTAACCaa          >  2:1518966/3‑150 (MQ=255)
                                                                                                                                            agccTGCGCAGATGCGCAGGCTGGTCTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACATTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGATTCGCAAACTGTAACCaa          >  1:498912/3‑150 (MQ=255)
                                                                                                                                            aCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCaaa         <  2:1533519/150‑1 (MQ=255)
                                                                                                                                               cTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAgg       >  2:1443357/1‑150 (MQ=255)
                                                                                                                                                   gcATCTTCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGTATCACCACTTTAGCAACCTGAAGCCATAAGCCACCTGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAATGTTTg   <  1:168892/150‑1 (MQ=255)
                                                                                                                                                    cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc  >  2:1564721/1‑150 (MQ=255)
                                                                                                                                                    cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACACGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCCCAAAGTGTAACCAAAGGTTTGc  >  2:768239/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGC  >  NC_000913/2976172‑2976467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: