Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,993,889 T→A 7.1% intergenic (+33/‑50) ygeI → / → pbl uncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8890TA 7.1% 441.2 / 13.6 154intergenic (+33/‑50)ygeI/pbluncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family
Reads supporting (aligned to +/- strand):  ref base T (70/72);  new base A (7/5);  total (77/77)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 160 total aligned reads displayed.

TACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAA  >  NC_000913/2993742‑2994038
                                                                                                                                                   |                                                                                                                                                     
tACTGATACAGATCAAATACAAGCATTTATATTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                                                                     <  1:1663749/150‑1 (MQ=255)
tACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                                                                     <  2:1903667/150‑1 (MQ=255)
 aCTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                                                                    <  2:422743/150‑1 (MQ=255)
 aCTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                                                                    <  2:343817/150‑1 (MQ=255)
  cTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTtat                                                                                                                                                   >  2:562654/1‑150 (MQ=255)
    gATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTataa                                                                                                                                                 >  2:1519939/1‑150 (MQ=255)
        cAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                                                                             <  2:1275296/150‑1 (MQ=255)
         aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                            >  1:1884584/1‑150 (MQ=255)
         aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                            >  2:959079/1‑150 (MQ=255)
         aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                            <  1:548806/150‑1 (MQ=255)
          gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTataa                                                                                                                                                 >  2:293089/1‑144 (MQ=255)
          gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTataa                                                                                                                                                 <  1:293089/144‑1 (MQ=255)
            tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAtct                                                                                                                                         <  2:1675504/150‑1 (MQ=255)
              aaaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                                                                       >  2:1521015/1‑150 (MQ=255)
               aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGt                                                                                                                                      >  2:409595/1‑150 (MQ=255)
                 tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                    <  2:763289/150‑1 (MQ=255)
                 tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                    <  1:1463772/150‑1 (MQ=255)
                   cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                  >  1:815828/1‑150 (MQ=255)
                   cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                  >  1:1279319/1‑150 (MQ=255)
                    aaGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttatga                                                                                                                                 <  2:1018125/150‑5 (MQ=255)
                       cATTTATAGTTTCAACATGGATGGCGACTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                                                                              <  1:289914/150‑1 (MQ=255)
                         tttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                                                                            <  2:583557/150‑1 (MQ=255)
                           tataGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                                                                          <  2:1281925/150‑1 (MQ=255)
                           tataGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                                                                          >  2:1180987/1‑150 (MQ=255)
                               ttttCAAAATGGATGGCGACTTTTCAAAATGATATGTATTCAGAAGATAAACCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                                                                      <  1:159074/149‑1 (MQ=255)
                               gTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACATTACTAAAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTACTTTCATTCAATcc                                                                                                                      >  1:1925254/1‑150 (MQ=255)
                                 ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATACTATCTCACCTTACTATAAAATTGAGTGGTAGTCGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCtg                                                                                                                    <  1:273468/150‑2 (MQ=255)
                                  tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCAACTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTca                                                                                                                   <  1:345197/150‑1 (MQ=255)
                                   cAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcac                                                                                                                  >  2:1986577/1‑150 (MQ=255)
                                   cAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcac                                                                                                                  >  1:1938443/1‑150 (MQ=255)
                                    aaCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCTTTCCTTTCATTCAATCCTcaca                                                                                                                 >  2:390877/1‑150 (MQ=255)
                                      cATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGAACAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcacaca                                                                                                               >  2:840988/1‑150 (MQ=255)
                                         ggatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTGCCTTTCATTCAATCCTCACACATGa                                                                                                            >  1:1030579/1‑150 (MQ=255)
                                          gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaa                                                                                                           <  1:1843996/150‑1 (MQ=255)
                                            tggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaaaa                                                                                                         >  2:915880/1‑150 (MQ=255)
                                               cgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGATATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAat                                                                                                      <  1:1683588/150‑1 (MQ=255)
                                               cgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAat                                                                                                      <  1:849574/150‑1 (MQ=255)
                                                gcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAata                                                                                                     >  2:1062149/1‑150 (MQ=255)
                                                 ccTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAatat                                                                                                    >  2:946782/1‑150 (MQ=255)
                                                  cTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATg                                                                                                   >  2:1756582/1‑150 (MQ=255)
                                                  attttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATg                                                                                                   <  1:431483/149‑1 (MQ=255)
                                                   ttttCAAAATGATATGTATTCAGAATATAATCCTATCTCACCTTACTAAAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATgc                                                                                                  <  1:1037705/150‑1 (MQ=255)
                                                       cAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAATAGTATCTCGTTCCTTTCATTCAATACTCACACATGAAAAAATATGCGCTc                                                                                              >  1:800186/1‑150 (MQ=255)
                                                       cAAAATGATATGTAATCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAAAAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                              >  1:1004444/1‑150 (MQ=255)
                                                       aaaaaTGTTATGTATTCAGAAGTTAATCATATCTCACCTTACTATATAATTGATTGGTAGACGATTGACCAAATAAATCATTACGAGATACTTTTATAATAGTATCTCGTTCCTTTAATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                              <  1:158598/149‑1 (MQ=255)
                                                       aaaaaTGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                              <  1:1366029/149‑1 (MQ=255)
                                                         aaaTGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCAc                                                                                            >  1:1635292/1‑150 (MQ=255)
                                                                 tGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                                                                                    <  2:1166345/150‑1 (MQ=255)
                                                                  ttATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                   <  1:1505646/149‑1 (MQ=255)
                                                                      tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAtt                                                                               >  1:711451/1‑150 (MQ=255)
                                                                           agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGAGCTAACCAAAAGTTAAATTCTTaa                                                                          >  1:381555/1‑150 (MQ=255)
                                                                              tAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAGAAGTTATATTCTTGACCa                                                                       >  1:1330981/1‑150 (MQ=255)
                                                                                aTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAACTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATc                                                                     >  1:769130/1‑150 (MQ=255)
                                                                                 tCCTATCTCACCTTACTTTAAAATTGAGTGGTAGTCGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTGCTTTCATTCTATCCTCTCACATGAAATAATATGCGCTCACCAAAAGTTAAATTCTTATGCATCt                                                                    <  2:365049/150‑1 (MQ=255)
                                                                                 tCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCt                                                                    >  1:1778938/1‑150 (MQ=255)
                                                                                   cTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTc                                                                  <  2:861642/150‑1 (MQ=255)
                                                                                         cACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGttt                                                            >  2:290369/1‑150 (MQ=255)
                                                                                         cACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGttt                                                            >  2:981498/1‑150 (MQ=255)
                                                                                         cACCTTACTAAAAAATTGAGTGGAAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTACCTTTCATTCAATCCTCACACATCAAAAAAAAAGCCCTCACCAAAAGTAAAATACTAAACAAACTACACGttt                                                            >  2:745837/1‑150 (MQ=255)
                                                                                          aCCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtttt                                                           <  2:1854627/150‑1 (MQ=255)
                                                                                          aCCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtttt                                                           >  1:919198/1‑150 (MQ=255)
                                                                                          aCCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGttta                                                           <  1:1180987/150‑2 (MQ=255)
                                                                                             ttACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGta                                                        <  2:1609801/150‑1 (MQ=255)
                                                                                               aCTATAAAATTGAGTGGTATACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtata                                                      <  1:833175/150‑1 (MQ=255)
                                                                                               aCTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtata                                                      <  1:1329995/150‑1 (MQ=255)
                                                                                                cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                     >  1:1239151/1‑150 (MQ=255)
                                                                                                atataAAATTGAGTGGTAGACGTTTGACCAAATAAATCTTAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                     <  2:1334988/149‑1 (MQ=255)
                                                                                                  ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt                                                   >  1:1763408/1‑150 (MQ=255)
                                                                                                  ataAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt                                                   <  1:1986577/150‑1 (MQ=255)
                                                                                                    aaaaATGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTGCTTTCATTCAATCCACACACATGAAAAAATAACCGCTCCCCAAAAGATAAATTCTTAAACATCTTCAGGTTTTGtaatttttt                                                 >  2:124265/1‑143 (MQ=255)
                                                                                                      aaTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATttatta                                               <  1:1824810/150‑1 (MQ=255)
                                                                                                       aTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTAc                                              <  2:733301/150‑1 (MQ=255)
                                                                                                        ttGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACa                                             >  2:1361936/1‑150 (MQ=255)
                                                                                                             tGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAAt                                        <  2:1991803/150‑1 (MQ=255)
                                                                                                              tgTAGACGATTGACCAAATAAATCATAACGAGATAATTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAATTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATg                                       <  1:1285232/149‑1 (MQ=255)
                                                                                                               gTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGt                                      >  1:534278/1‑150 (MQ=255)
                                                                                                                tAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTc                                     >  1:1505769/1‑150 (MQ=255)
                                                                                                                     taTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCg                                <  1:673131/149‑1 (MQ=255)
                                                                                                                      aTTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTATTACAAAAATGTCTTTCGcc                              <  2:1710884/150‑1 (MQ=255)
                                                                                                                       ttGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGcc                              >  2:101509/1‑150 (MQ=255)
                                                                                                                        tGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCa                             <  2:1559013/150‑1 (MQ=255)
                                                                                                                            cAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCaa                         >  2:1302096/1‑150 (MQ=255)
                                                                                                                             aaataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAc                        <  1:948978/150‑1 (MQ=255)
                                                                                                                              aataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAct                       >  1:238884/1‑150 (MQ=255)
                                                                                                                                  aatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTg                   <  1:1062149/150‑1 (MQ=255)
                                                                                                                                   atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                  >  2:1467156/1‑150 (MQ=255)
                                                                                                                                     aaTAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctc                <  2:79790/149‑1 (MQ=255)
                                                                                                                                      ggaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACTCATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctct               >  2:1251477/3‑150 (MQ=255)
                                                                                                                                      aTAACGAGATACTTTTATAATAGTATCTAGGTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATAATCGCGTATTGAATATTTATTAAAAAAATGTATTTCGCCAGCAACTCTTGctat               >  1:1366883/1‑148 (MQ=255)
                                                                                                                                         aCGAGATACTTTTATAATAGTTTCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAAt            <  2:836866/150‑1 (MQ=255)
                                                                                                                                          cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg           <  2:1127404/150‑1 (MQ=255)
                                                                                                                                          cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg           <  1:1157517/150‑1 (MQ=255)
                                                                                                                                             gaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAg        <  1:840988/150‑1 (MQ=255)
                                                                                                                                              aTACTTTTATAATAGTATCTCGTTCATTTCATTCAATCCTAACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGc       <  1:1297273/150‑1 (MQ=255)
                                                                                                                                               tACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTGTTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCa      <  2:680744/150‑1 (MQ=255)
                                                                                                                                               tACATTAATAATAGTATCTCGTTCCATTCATTCAATCCTCACACATGAAAAATTATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTAGCCAGCAAATCTTGCTCTAATGAACCa      >  2:1073206/1‑150 (MQ=255)
                                                                                                                                                aCTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTGACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAg     >  2:912404/1‑150 (MQ=255)
                                                                                                                                                 cTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGc       <  1:909981/147‑1 (MQ=255)
                                                                                                                                                 attttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAgg    <  2:1657190/149‑1 (MQ=255)
                                                                                                                                                   tttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGaa  >  2:821984/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAA  >  NC_000913/2993742‑2994038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: