Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,993,896 T→A 7.3% intergenic (+40/‑43) ygeI → / → pbl uncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,993,8960TA 7.3% 380.1 / 16.5 151intergenic (+40/‑43)ygeI/pbluncharacterized protein/pseudogene, peptidoglycan‑binding enzyme family
Reads supporting (aligned to +/- strand):  ref base T (70/70);  new base A (6/5);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 158 total aligned reads displayed.

ATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993747‑2994045
                                                                                                                                                     |                                                                                                                                                     
   cAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                                                                                    >  2:1674906/1‑150 (MQ=255)
    aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   <  1:628178/150‑1 (MQ=255)
    aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   >  2:959079/1‑150 (MQ=255)
    aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   <  1:548806/150‑1 (MQ=255)
    aGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   >  1:1884584/1‑150 (MQ=255)
    aGATAAAATACAATCATTTATATTTTCATCATGGATGTCGCCTTTTCAAAATGATATTTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGTTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTa                                                                                                                                                   <  1:183216/150‑1 (MQ=255)
     gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                                                                                  >  2:1748981/1‑150 (MQ=255)
       tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTTtct                                                                                                                                                >  1:836866/1‑150 (MQ=255)
       tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAtct                                                                                                                                                <  2:1675504/150‑1 (MQ=255)
          aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGt                                                                                                                                             <  1:1813035/150‑1 (MQ=255)
            tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                           <  2:763289/150‑1 (MQ=255)
            tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTc                                                                                                                                           <  1:1463772/150‑1 (MQ=255)
             aCAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttat                                                                                                                                          <  2:1033136/150‑3 (MQ=255)
             aCAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttat                                                                                                                                          <  2:753360/150‑3 (MQ=255)
              cAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCt                                                                                                                                         >  1:1279319/1‑150 (MQ=255)
               aaGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAAAAGTATCTCGttatga                                                                                                                                        <  2:1018125/150‑5 (MQ=255)
                  cATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                                                                                     >  2:538170/1‑150 (MQ=255)
                  cATTTATAGTTTCAACATGGATGGCGACTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                                                                                     <  1:289914/150‑1 (MQ=255)
                    tttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                                                                                   <  2:583557/150‑1 (MQ=255)
                      tataGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                                                                                 >  2:1180987/1‑150 (MQ=255)
                      tataGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattca                                                                                                                                 <  2:1281925/150‑1 (MQ=255)
                            ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATACTATCTCACCTTACTATAAAATTGAGTGGTAGTCGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCtg                                                                                                                           <  1:273468/150‑2 (MQ=255)
                              cAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcac                                                                                                                         >  1:1938443/1‑150 (MQ=255)
                              cAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcac                                                                                                                         >  2:1986577/1‑150 (MQ=255)
                                 cATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGAACAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcacaca                                                                                                                      >  2:840988/1‑150 (MQ=255)
                                    ggatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTGCCTTTCATTCAATCCTCACACATGa                                                                                                                   >  1:1030579/1‑150 (MQ=255)
                                       tggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaaaa                                                                                                                >  2:915880/1‑150 (MQ=255)
                                        ggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGaaaaa                                                                                                               >  2:1646702/1‑150 (MQ=255)
                                          cgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGATATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAat                                                                                                             <  1:1683588/150‑1 (MQ=255)
                                            ccTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAatat                                                                                                           >  2:946782/1‑150 (MQ=255)
                                             cTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATg                                                                                                          >  2:1756582/1‑150 (MQ=255)
                                              ttttCAAAATGATATGTATTCAGAATATAATCCTATCTCACCTTACTAAAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATgc                                                                                                         <  1:1037705/150‑1 (MQ=255)
                                                  cAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTATTATAATAGTATCTCGTTCCTTTCATTCAATACTCACACATGAAAAAATATGCGCTc                                                                                                     >  1:800186/1‑150 (MQ=255)
                                                  cAAAATGATATGTAATCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAAAAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                                     >  1:1004444/1‑150 (MQ=255)
                                                  aaaaaTGTTATGTATTCAGAAGTTAATCATATCTCACCTTACTATATAATTGATTGGTAGACGATTGACCAAATAAATCATTACGAGATACTTTTATAATAGTATCTCGTTCCTTTAATTCAATCCTCACACATGAAAAAATATGCGCTc                                                                                                     <  1:158598/149‑1 (MQ=255)
                                                    aaaTGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCAc                                                                                                   >  1:1635292/1‑150 (MQ=255)
                                                             ttATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                          <  1:1505646/149‑1 (MQ=255)
                                                             ttATTCAGAAGATAATCCTATATAAACTTAATATAAAATTGAGTGGTAGACGATTGAACAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                          <  1:1957474/149‑1 (MQ=255)
                                                             gTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                                                                          >  1:832157/1‑150 (MQ=255)
                                                               aTTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTaaa                                                                                        >  1:680084/1‑150 (MQ=255)
                                                                 tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAAtt                                                                                      >  1:711451/1‑150 (MQ=255)
                                                                  cAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTc                                                                                     <  2:403340/150‑1 (MQ=255)
                                                                      agaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGAGCTAACCAAAAGTTAAATTCTTaa                                                                                 >  1:381555/1‑150 (MQ=255)
                                                                         tAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAGAAGTTATATTCTTGACCa                                                                              >  1:1330981/1‑150 (MQ=255)
                                                                           aTCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATc                                                                            >  1:879925/1‑150 (MQ=255)
                                                                            tCCTATCTCACCTTACTTTAAAATTGAGTGGTAGTCGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTGCTTTCATTCTATCCTCTCACATGAAATAATATGCGCTCACCAAAAGTTAAATTCTTATGCATCt                                                                           <  2:365049/150‑1 (MQ=255)
                                                                            tCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCt                                                                           >  1:1778938/1‑150 (MQ=255)
                                                                              cTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTc                                                                         <  2:861642/150‑1 (MQ=255)
                                                                              cTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTc                                                                         <  2:1266723/150‑1 (MQ=255)
                                                                                   tcACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTAACCAAAAGTTAAATTCTTAACCATCTTCACGtt                                                                    >  1:1716563/1‑150 (MQ=255)
                                                                                    cACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGttt                                                                   >  2:290369/1‑150 (MQ=255)
                                                                                    cACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGttt                                                                   >  2:981498/1‑150 (MQ=255)
                                                                                      ccTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg                                                                 >  1:304708/1‑150 (MQ=255)
                                                                                       cTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGt                                                                <  1:1990104/150‑1 (MQ=255)
                                                                                        ttACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGta                                                               <  2:1609801/150‑1 (MQ=255)
                                                                                         tACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtat                                                              <  1:959079/150‑1 (MQ=255)
                                                                                          aCTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtata                                                             <  1:1329995/150‑1 (MQ=255)
                                                                                           cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            >  1:1594905/1‑150 (MQ=255)
                                                                                           cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            >  1:1239151/1‑150 (MQ=255)
                                                                                           cTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            >  1:1169215/1‑150 (MQ=255)
                                                                                           atataAAATTGAGTGGTAGACGTTTGACCAAATAAATCTTAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat                                                            <  2:1334988/149‑1 (MQ=255)
                                                                                                  aTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACTTGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTAc                                                     <  2:352480/150‑1 (MQ=255)
                                                                                                  aTTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTAc                                                     <  2:733301/150‑1 (MQ=255)
                                                                                                   ttGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACa                                                    >  2:1361936/1‑150 (MQ=255)
                                                                                                        tGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAAt                                               <  2:1991803/150‑1 (MQ=255)
                                                                                                         tgTAGACGATTGACCAAATAAATCATAACGAGATAATTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAATTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATg                                              <  1:1285232/149‑1 (MQ=255)
                                                                                                          gTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGt                                             >  2:1232895/1‑150 (MQ=255)
                                                                                                          gTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGt                                             >  2:1554293/1‑150 (MQ=255)
                                                                                                           tAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTc                                            <  2:1279319/150‑1 (MQ=255)
                                                                                                           tAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTc                                            <  1:797178/150‑1 (MQ=255)
                                                                                                           tAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTc                                            >  1:1505769/1‑150 (MQ=255)
                                                                                                                    gACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAg                                   >  2:1103666/1‑150 (MQ=255)
                                                                                                                    gACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAg                                   >  1:363474/1‑150 (MQ=255)
                                                                                                                     aCCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGc                                  <  1:1319784/150‑1 (MQ=255)
                                                                                                                         aataaatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAAct                              >  1:238884/1‑150 (MQ=255)
                                                                                                                             aatCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTg                          <  1:1062149/150‑1 (MQ=255)
                                                                                                                              atCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGc                         >  2:1467156/1‑150 (MQ=255)
                                                                                                                                aaTAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctc                       <  2:79790/149‑1 (MQ=255)
                                                                                                                                 ggaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACTCATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctct                      >  2:1251477/3‑150 (MQ=255)
                                                                                                                                 ggaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctct                      >  1:474852/3‑150 (MQ=255)
                                                                                                                                 aTAACGAGATACTTTTATAATAGTATCTAGGTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATAATCGCGTATTGAATATTTATTAAAAAAATGTATTTCGCCAGCAACTCTTGctat                      >  1:1366883/1‑148 (MQ=255)
                                                                                                                                  tAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTa                     <  2:1141196/150‑1 (MQ=255)
                                                                                                                                  gaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTa                     >  2:1901194/2‑150 (MQ=255)
                                                                                                                                  gaaCGAGATACTATTATAAAAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTACTCTa                     >  1:1910837/2‑150 (MQ=255)
                                                                                                                                    aCGAGATACTTTTATAATAGTTTCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAAt                   <  2:836866/150‑1 (MQ=255)
                                                                                                                                     cGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATg                  <  1:1157517/150‑1 (MQ=255)
                                                                                                                                      gagaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAAGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGa                 >  1:948166/1‑150 (MQ=255)
                                                                                                                                        gaTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAg               <  1:840988/150‑1 (MQ=255)
                                                                                                                                         aTACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGc              <  2:572230/150‑1 (MQ=255)
                                                                                                                                         aTACTTTTATAATAGTATCTCGTTCATTTCATTCAATCCTAACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGc              <  1:1297273/150‑1 (MQ=255)
                                                                                                                                          tACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTGTTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCa             <  2:680744/150‑1 (MQ=255)
                                                                                                                                          tACATTAATAATAGTATCTCGTTCCATTCATTCAATCCTCACACATGAAAAATTATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTAGCCAGCAAATCTTGCTCTAATGAACCa             >  2:1073206/1‑150 (MQ=255)
                                                                                                                                           aCTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTGACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAg            >  2:912404/1‑150 (MQ=255)
                                                                                                                                            cTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGc              <  1:909981/147‑1 (MQ=255)
                                                                                                                                             ttttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGa          <  2:1505769/150‑1 (MQ=255)
                                                                                                                                              tttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGaa         >  2:821984/1‑150 (MQ=255)
                                                                                                                                               ttATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAAc        >  2:1000688/1‑150 (MQ=255)
                                                                                                                                                tataATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACa       <  2:268649/150‑1 (MQ=255)
                                                                                                                                                  taataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGctc                       <  2:1322756/132‑1 (MQ=255)
                                                                                                                                                   aataGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATg    >  1:1403854/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTTATTACAAAAATGTCTTTCGCCAGCAACTCTTGCTCTAATGAAGCAGGAACAATGTT  >  NC_000913/2993747‑2994045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: